Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,034,564 C→A 9.5% intergenic (‑230/‑164) EO946_RS10175 ← / → EO946_RS10180 UPF0715 family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,034,5640CA 9.5% 348.3 / 2.9 146intergenic (‑230/‑164)EO946_RS10175/EO946_RS10180UPF0715 family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (103/21);  new base A (2/18);  total (107/39)
Fisher's exact test for biased strand distribution p-value = 2.30e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

ATTTTGTTCATTTTTTCACCTCCTAATTATTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAA  >  NZ_CP034943/2034425‑2034702
                                                                                                                                            |                                                                                                                                           
aTTTTGTTCATTTTTTCACCTCCTAATTATTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGATTATTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTATAGTCAGCttcttc                                                                                                                                             >  2:2682216/1‑140 (MQ=255)
 ttttGTTCATTTTTTCACCTCCTAATTATTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACATTGTTTCCCAGCTTTAGTCAGCTTCTTCa                                                                                                                                            >  2:5201970/1‑140 (MQ=255)
 ttttGTTCATTTTTTCACCTCCTAATTATTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCa                                                                                                                                            >  1:3272974/1‑140 (MQ=255)
         aTTTTTTCACCTCCTAATTATTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGaa                                                                                                                                    <  1:3320099/140‑1 (MQ=255)
         aTTTTTTCACCTCCTAATTATTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGaa                                                                                                                                    <  1:917755/140‑1 (MQ=255)
                             tttAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTc                                                                                                                >  1:3826973/1‑140 (MQ=255)
                                aaTGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTATTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCaaa                                                                                                             >  2:3615741/1‑140 (MQ=255)
                                aaTGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACTAAGTGTGCTAATAAAATGTTTCaaa                                                                                                             >  2:4658043/1‑140 (MQ=255)
                                aaTGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCaaa                                                                                                             >  2:792275/1‑140 (MQ=255)
                                      gggtGTTATGTTTACATGCAGAAGCGTCCCCTCCCCTGTCCTTATCTCCGTTTCTTTTACAAACGTTTTGTACAGTGTTTCCCAGCTATCGTCAGCTTCTTAAAATACTATGTGTGCTAATAAATTGTTTAAAAAACGAc                                                                                                      <  2:2224041/137‑1 (MQ=255)
                                      ggCTTTTTTTTTTAATTGCATATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACTTTTTGTACTGTTTTTCCCAGCTTTAGTCAGCTTCTTAATATACGAAGTGTGATAATAAAATTTTTAAAAAACGAc                                                                                                      <  2:5062049/140‑1 (MQ=255)
                                                 ttACTTGCAGTTTCGTTCCCTCCCGTGTCTTTAACCCCGATTATTTTAAAACCTTTTTGAACAGTGTTACCCCGCTTTAGTCATTTTCTAAATATACGAAGTGTGAAAATAAATTGTATCAAAAACGACCGGGGTAAAGc                                                                                           <  2:166704/140‑1 (MQ=255)
                                                   aCATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTg                                                                                         >  2:5095748/1‑140 (MQ=255)
                                                   aCATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAAAGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACTACCGGGGTAAAGCTg                                                                                         >  2:3911609/1‑140 (MQ=255)
                                                    cttGCAGATTCCGTCCCAACCCGGCCCTGCGCGCCGCTTTTTTTTAAATGGTTTTGTACTGTTTTCCCCAGCTTTAGTCAGCTTCTTAATATAGGATGTGTTCTAATAAATTTTTACAAAAACGACCGGGGTAAAGCTGa                                                                                        <  2:830896/138‑1 (MQ=255)
                                                    attGCAGATTCTTTCCCTAACGGGTATTTAGCTCGGTTTCTTTTTAAAACTTTTTGAACTGTTTTTCCCATGTTTAGTCAGATTCTTATTATACTATGTGTGTTATTAAATTTTTACAAACACGACCGGGGTAAAGCTGa                                                                                        <  2:1112367/138‑1 (MQ=255)
                                                     aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGACTTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGAc                                                                                       >  1:4991644/1‑140 (MQ=255)
                                                     aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATCAAATGTTTCAAAAACGACCGGGGTAAAGCTGAc                                                                                       >  1:4806166/1‑140 (MQ=255)
                                                     aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGGGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGAc                                                                                       >  1:3366726/1‑140 (MQ=255)
                                                     aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGATTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTACTAAATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGAc                                                                                       >  1:2181034/1‑140 (MQ=255)
                                                          gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                  >  2:709486/1‑140 (MQ=255)
                                                             tctttccATAACCTTTCATTATCTCCGCTTCTTTTTAAAACTTTTTGTACTGTTTTTCCCAGCTTTAGTCAGCTTCTTAATATACTAATTGTGATAATAAAATGTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGt                                                                               <  2:2733074/137‑1 (MQ=255)
                                                               cttccCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTGTTGTACAGTGTGTCCCAGCTTTAGTCCGCTTCTTCATATACGCAGTGGGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGtgt                                                                             >  1:4181517/1‑138 (MQ=255)
                                                                  cccTAAGGTTTCCTTAGATCCTATTCTTTTTCAAAGTTTTTGTACTTTGTTTCCCTGCTTTTGTCTGGTTTTTAATTTACCATGTGTGCTAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTaa                                                                          <  2:3826973/140‑1 (MQ=255)
                                                                  cccTAACGTCTCCTTATCACCTCTTATTTTTCAAACTTTTTTTACAGTTTTTCCCAGATTTATTCAGATTTTTCATAAACCAATTGTGATAATAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTaa                                                                          <  2:3617963/140‑1 (MQ=255)
                                                                  cccTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATTTTTCAAAAACGACCGGGGTAAATCTGACTCTCTTGTCTTaa                                                                          >  1:3640553/1‑140 (MQ=255)
                                                                       cccggTCCTTATCGCCGCTTTTTTTACAAACGTTTGGTACAGTGTTTCCCCGCTTGAGACAGCTTCTAAATATACGACTTGGGCTAATAAAATGTTACAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGa                                                                     <  2:1035551/136‑1 (MQ=255)
                                                                       aCCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGa                                                                     >  1:1076906/1‑140 (MQ=255)
                                                                       aCCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTATTGTCTTAATGGGa                                                                     >  2:4550711/1‑140 (MQ=255)
                                                                       aCCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGAATCTCTTGTCTTAATGGGa                                                                     >  1:20734/1‑140 (MQ=255)
                                                                           gTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAaga                                                                 >  1:781127/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTTTTTCCCAGCTTTAGTCAGCTTCTTCATATAAGAAGTGGGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  1:610017/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGTGAAagag                                                                >  2:21305/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAcgag                                                                >  1:1370434/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  2:4241470/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  1:1035551/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  2:251771/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  1:2496035/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  1:584480/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  2:4242622/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCGTGTCTTAATGGGACagag                                                                >  1:3488457/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGGACAGTGTTTACCAGCTTTAGTCAGCTTATTCATATACCAAGTGTGCGAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                >  1:498139/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTGTTTCAAACGTTTTGTACAGTGTTTCCCCGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACAGGGGTAAACCTGACTCTCTTGTCTTAATCGGAAagag                                                                >  2:2982888/1‑140 (MQ=255)
                                                                            tCCTTATCTCCGCTTCTATTTCAAACGATTTGTACAGTGTTTCCCAGCTTTACTCAGCTTCTTCATATACGAAGTTTGCGAATAAAATTTTTCAAAAAACACAGGGGTAAATCTGACTCTCTTGTCGTCATGGGACagag                                                                >  1:2691513/1‑140 (MQ=255)
                                                                                 aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAACGAGTCAAc                                                           >  2:1053932/1‑140 (MQ=255)
                                                                                 aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAAc                                                           >  2:3319729/1‑140 (MQ=255)
                                                                                 aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAAc                                                           >  1:2396530/1‑140 (MQ=255)
                                                                                 aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAAc                                                           >  1:1086/1‑140 (MQ=255)
                                                                                 aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCGGACTCTCTGGTCTGAATGGGAAAGAGTCAAc                                                           >  1:3677504/1‑140 (MQ=255)
                                                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTGTTAATGGGAAAGAGTCAACGaaaa                                                      >  1:2439572/1‑140 (MQ=255)
                                                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAACGAGTCAACGaaaa                                                      >  2:3538473/1‑140 (MQ=255)
                                                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                      >  1:5180933/1‑140 (MQ=255)
                                                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                      >  1:2396572/1‑140 (MQ=255)
                                                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                      >  1:4275918/1‑140 (MQ=255)
                                                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAACGGGGTAAAGCTGACTCTATTTTGTTAATGGGAAAGATTAAACGaaaa                                                      >  2:1492525/1‑140 (MQ=255)
                                                                                      cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCGAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTGTTGGCTTAATGGGAAAGAGTCAACGgaaa                                                      >  1:2926902/1‑140 (MQ=255)
                                                                                        cttcttTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaaaa                                                    >  2:5100332/1‑140 (MQ=255)
                                                                                          tttttttCAACCTTTTTGTACCGTGTTTCCAAGCTTTCGTCATCTTCTGCATATCCGAGGTGTGATAAAAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAg                                                  <  2:2396572/138‑1 (MQ=255)
                                                                                          tttttttAAAAGTTTTTGTAGTGTGTTACCCTGTTTTAGCAAGTTTCTTATTTTAGTAGTTTTGCTAAAAAATTTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAg                                                  <  1:5017443/138‑1 (MQ=255)
                                                                                           ttttttCAAACGTTTTTTAATGTGTTTCCCAGATTTTGTCAGTTTATTCATATACCACGTGTGATATTAAAATTTTTCAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGc                                                 <  2:3289040/139‑1 (MQ=255)
                                                                                           tattttAAAACCGTTTTTCACTGTGTTTCCCACCTTTCGACAGATTCTAAATATACGAAGTGTCATAAAAAAATTTAACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAg                                                  <  1:3150155/138‑1 (MQ=255)
                                                                                           cttTTCCAAACGTTTTTTACTGTGTTTCCCAGATTTAGTCAGATTATACATATACGAAGTGTGCTAATAATATTTTTCAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGc                                                 <  2:3281564/140‑1 (MQ=255)
                                                                                             ttttCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCga                                               >  1:825187/1‑140 (MQ=255)
                                                                                                           tGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGcc                                  >  2:4659509/1‑140 (MQ=255)
                                                                                                                 gtgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacag                            >  1:972010/1‑140 (MQ=255)
                                                                                                                 gtgtTTCCCAGCTTTAGTCAGCTTCTCAATATACGATGTTTGGTAATAAAATGTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacag                            <  2:1074470/140‑1 (MQ=255)
                                                                                                                  tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                           >  1:2373834/1‑140 (MQ=255)
                                                                                                                  tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                           >  2:351797/1‑140 (MQ=255)
                                                                                                                  tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                           >  1:2007018/1‑140 (MQ=255)
                                                                                                                   ttttCCCAGTTTTCGTCAGTTTTTTATTATCCGAAGTGTGATAATAAATTTTTCAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                          <  2:1824730/139‑1 (MQ=255)
                                                                                                                   ttttCCCAGTTTTATTAAGATACTTAAAATCCTAATTTTGAAAAAAAAATTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                          <  1:5107268/139‑1 (MQ=255)
                                                                                                                    tttCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagaca                         >  2:110873/1‑140 (MQ=255)
                                                                                                                       cccAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAACGCGAATGAATACCTGCCTAACAGACAGaa                      >  1:4584097/1‑140 (MQ=255)
                                                                                                                       cccAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGACTACCTGCCTATCAGTCAGaa                      >  1:3978081/1‑140 (MQ=255)
                                                                                                                       cccAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGaa                      >  1:3200230/1‑140 (MQ=255)
                                                                                                                       cccAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGaa                      >  2:3467536/1‑140 (MQ=255)
                                                                                                                        ccATCTTTAGTTCTCTTCTTCTTATACGCTGTGTGATAATAAATTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAAt                     <  2:3178989/140‑1 (MQ=255)
                                                                                                                        acctgtttACTAAGCTTCTTATTATACTATGGGTGCTAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAAt                     <  2:406507/135‑1 (MQ=255)
                                                                                                                           gattttGTCCGCTTATTCATAAACTACGTGTTAAAATAAATTTTTAAAACAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaag                  <  1:3528279/138‑1 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATCCGAAGTGTGCGAATAACATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTGTTGTCTTAATGGGAAAGAGTCAACGAAAACTAGCGCATGAATACCTGCGTAACAGACAGAATaaga                 >  2:3943363/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAATACGAGCGGGGTAAAGCTGACTCTCTTGTCTTAATGTGAAAGAGTCAACGAAAAAAAGCGAATGAAAACCTGCCTAACAGACAGAATaaga                 >  1:1211392/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGACAGAGTCAACGAAAAAAAGCGAATGAATACCTGCGTACCAGACAGAATaaga                 >  1:2884710/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGACTACCTGCCTATCAGACAGAATaaga                 >  2:5187196/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGACTaaga                 >  2:3773742/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                 >  2:2130471/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                 >  2:4058748/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                 >  2:85230/1‑140 (MQ=255)
                                                                                                                            cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                 >  1:4834815/1‑140 (MQ=255)
                                                                                                                             tttAGACAGTTTCTCAATATACCATTTGTCATAAAAAAATTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaagaa                <  1:1602503/140‑1 (MQ=255)
                                                                                                                                aGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGACTAAGAagag             >  1:2956275/1‑140 (MQ=255)
                                                                                                                                aGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAagag             >  1:3670937/1‑140 (MQ=255)
                                                                                                                                aGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAagag             >  1:2770104/1‑140 (MQ=255)
                                                                                                                                aGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAagag             >  1:4953563/1‑140 (MQ=255)
                                                                                                                                 gTCAGCTTCTTCATATACGAAGAGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGATGACTCTCTTGTCTTAAGGGGAAAGCGTCAACGAAAAAAAGCGAATGAATACCTGCCTACCCGACCTAATAAGAAGAGt            >  2:4974857/1‑140 (MQ=255)
                                                                                                                                 gTCAGATTCTTCATATCCGAAGTGTGATAATAAAATTTTACAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGt            <  2:2520800/140‑1 (MQ=255)
                                                                                                                                 ctCACCTTCTTAATATACGACGTTTCAAAATAAATTTTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGt            <  1:1604395/139‑1 (MQ=255)
                                                                                                                                   cAGATTCTTAATATACGATTTGTGATAATAAAATGTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTg          <  2:3301359/140‑1 (MQ=255)
                                                                                                                                      cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAATAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGata       >  2:1198970/1‑140 (MQ=255)
                                                                                                                                      cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGata       >  2:4492385/1‑140 (MQ=255)
                                                                                                                                           acATATCCGATGTGTTATAATAAAATGTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCaa  <  1:2589793/139‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
ATTTTGTTCATTTTTTCACCTCCTAATTATTTAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAA  >  NZ_CP034943/2034425‑2034702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: