Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,139,480 A→C 6.3% A119A (GCT→GCG deoD ← purine‑nucleoside phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,139,4800AC 6.3% 1103.0 / 11.5 425A119A (GCT→GCGdeoDpurine‑nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (256/123);  new base C (2/44);  total (258/167)
Fisher's exact test for biased strand distribution p-value = 1.35e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 428 total aligned reads displayed.

GCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATT  >  NZ_CP034943/2139344‑2139617
                                                                                                                                         |                                                                                                                                         
 cGATTCGTCGTTGTAGAACTGATCAGCTGTAAATAGGGTTCCTACCGTCACCGGAACGCCTTTTTCCTTTGGGGCATCTTAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAAACAATAGTTCCGAACGCGa                                                                                                                                       <  2:1187497/140‑1 (MQ=255)
   aaTCGTCTTTGTACACCTGATCAGCGTTAAATACGCTTCCTCCCGCCCCCGGCACGCCTTTATCCTTTGCGGCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATAAATACTTCCCAAAGCGAct                                                                                                                                     <  1:4078889/140‑1 (MQ=255)
   aaTCGTCGTTGTAGAACTGATCAGCTTTAAATACGCTTCCTACCGTCACCGGCCCGCCTTTATCCTTTGCGGCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATAAATACTTCCGAAAGCGAct                                                                                                                                     <  2:3730379/140‑1 (MQ=255)
   aaTCGTCGTTGTACACCTGATCAGCTGTAAAAACGCTTCCTACCGCCACCGGCACGACTTTATCCTTTGCGCCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGAACGGCGAAAAATAAATACTTCCGAAAGCGAct                                                                                                                                     <  2:3885146/140‑1 (MQ=255)
        gggtTGTAGAACTCATCAGCTTTAAATACGCTACCTACCGACACCGCCACCCCTTTATCCTTGGCGCCATCATAGCCATTTTTTAAAAGCTCGAAATCTGCCCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGtt                                                                                                                                <  2:4448612/138‑1 (MQ=255)
            tGTATAACTGATCAGCTTTAAATCCGCTTCCTCCCGTCACCGGCACGCCTTTATCCTTTGCGGAATCATAGGAATTTTTAAAAAGCTCTAAATCGGCGCACGGCGAAAAATCAATACTACCCAACGCGACTCTGTTCAtt                                                                                                                            <  1:3942890/140‑1 (MQ=255)
                   cttctCAGCTGTAAATACGCTTCCTACGTGCCCGGCCACGCCTTTAGCCTGTGCGGAATCAGCGCCTTTTTTTAAAACCTCCAAATCGGCGCACGGCCCAAAATCAATCCGACCCAACGCGACTCTGTTCATTTGTGAAt                                                                                                                     <  1:568747/136‑1 (MQ=255)
                   cTGCTCAGGTGTAAATCCGCTTCCTCCCGTCCCCGGCACGCCTTTATCCTTTGCGGCATCATAGCCTTTTTTTAAACGCTCCAAATCTGCCAAAGGCGCAAAATCAATACTGCCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                     <  1:4984222/140‑1 (MQ=255)
                   cTGATCAGCTTTAAATAGGGTTCATACCGTCACGGGAACGCCTTTATCCTTTGCGGCATAATAGTCATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                     <  2:1877233/140‑1 (MQ=255)
                      aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:4508400/1‑140 (MQ=255)
                      aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  2:109374/1‑140 (MQ=255)
                      aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  2:599518/1‑140 (MQ=255)
                      aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:5198341/1‑140 (MQ=255)
                      aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  1:4002061/1‑140 (MQ=255)
                      aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                  >  2:3500519/1‑140 (MQ=255)
                           cTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAg                                                                                                             >  1:2070401/1‑140 (MQ=255)
                             gTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGa                                                                                                           >  2:3664627/1‑140 (MQ=255)
                                aaTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGt                                                                                                        >  2:2802187/1‑140 (MQ=255)
                                    cGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATa                                                                                                    >  1:4753774/1‑140 (MQ=255)
                                    cGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATa                                                                                                    >  1:342843/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCCACATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                   >  1:1146779/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAATTCAATACTTCCGAAAGCGACTCTGTTCATTTGTGACTCAGTTGTGGATGTCATAg                                                                                                   >  2:4724134/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGCGGAGGTCATAg                                                                                                   >  1:4285372/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCTACTCTGTTCATTTGTGAATAAGTTGAGGATGTCATAg                                                                                                   >  1:307182/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                   >  1:1084280/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                   >  1:2579924/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                   >  2:4842826/1‑140 (MQ=255)
                                     gCTTCCTACCGTCACCGGCACGCCGTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGACAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                   >  1:2323522/1‑140 (MQ=255)
                                      cTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATCGGATTTTTTTAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATCCTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGc                                                                                                  <  2:2480339/140‑1 (MQ=255)
                                       ttCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGcc                                                                                                 >  1:1780692/1‑140 (MQ=255)
                                        tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                >  1:1001599/1‑140 (MQ=255)
                                        tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                >  1:1232002/1‑140 (MQ=255)
                                        tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                >  1:888790/1‑140 (MQ=255)
                                        tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                >  1:439494/1‑140 (MQ=255)
                                                tCACCGGCACGCCTTTATCCGTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACGTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATCGCCAAAAGGACg                                                                                        >  1:3776241/1‑140 (MQ=255)
                                                 cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                       >  1:3124031/1‑140 (MQ=255)
                                                 cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                       >  1:5075131/1‑140 (MQ=255)
                                                 cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                       >  2:4016079/1‑140 (MQ=255)
                                                   ccGGCACGCCTTTCCCCTTGGCGGCAAAATCGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATCCTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                     <  2:5158636/140‑1 (MQ=255)
                                                   ccGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                     <  2:4223722/140‑1 (MQ=255)
                                                     ggCACGCCTTTATCCTTTGCGGCATCATTGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCg                                                                                   <  2:3809333/140‑1 (MQ=255)
                                                      gCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGCAAAATCAATCCTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGc                                                                                  <  2:2640487/140‑1 (MQ=255)
                                                        cACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTGCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCa                                                                                 >  2:2767568/1‑140 (MQ=255)
                                                           gCCTTTATCCTTGGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGCCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCAcgt                                                                              >  2:779824/1‑138 (MQ=255)
                                                              tttATCCTTTGCGGAATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCTAAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGa                                                                           <  2:1141115/140‑1 (MQ=255)
                                                              tttATCCTCCGCGTCATCATAGTCATTGTTCAAAAGCTCCCAATCGGCCCAAGCCGCAAAATAAAACCTCCCGAACCCGACTCTGTTCATTTGTGAATCAGTGTAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGa                                                                           <  2:1748262/140‑1 (MQ=255)
                                                               ttATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGAc                                                                          >  1:4828427/1‑140 (MQ=255)
                                                               ttATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGAc                                                                          >  1:954365/1‑140 (MQ=255)
                                                               ttATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAAAACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGAc                                                                          >  2:2044101/1‑140 (MQ=255)
                                                                tagccTTTGCCGCATCATATGAATTTTTTAAAAGCTCGAAATCTCCCCAAGGCGCAAAATCATTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                         <  2:2928218/137‑1 (MQ=255)
                                                                tATCCTTTGGGGCATCATCGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                         <  2:2235793/140‑1 (MQ=255)
                                                                tATCCTTTGCGGCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                         <  1:4877023/140‑1 (MQ=255)
                                                                tATCCTTTGCGGCATAATAGTAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                         <  2:4099662/140‑1 (MQ=255)
                                                                 aTCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACAt                                                                        >  2:1789318/1‑140 (MQ=255)
                                                                      ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTg                                                                   >  1:4216536/1‑140 (MQ=255)
                                                                      ttGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTg                                                                   >  1:4583435/1‑140 (MQ=255)
                                                                       ttcGGCATCATAGGCATTTTTTAAAATATCGAAATCTGCTCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACTTCCAGCACTTTGAAATCTTTGc                                                                  >  2:1924547/3‑140 (MQ=255)
                                                                       tgaggCATCATAGGGATTTTTTAAAAGCTCGAAATCTGCGCAATGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  2:1531461/4‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGGGCAAAAACAAAACTTACAAAACCTACTCTGTTAAAATGTGAATCAGTTGAGGATGTCATAGCCAAAATGAAGTCACTCACTTTGACATCATTga                                                                  >  2:5019539/1‑139 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGTATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCCGTTTGACATCTTTGc                                                                  >  1:1256921/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:2429852/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:1125883/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:3657007/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:284657/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  2:4918589/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:1887877/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:1655261/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:1438322/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  1:691581/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                  >  2:161406/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTATGACATCTTTGc                                                                  >  1:4331901/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCAGCTCTTTGACATCTTTGc                                                                  >  2:3118027/1‑140 (MQ=255)
                                                                               catAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                          >  1:4688186/1‑140 (MQ=255)
                                                                               catAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                          >  1:3177068/1‑140 (MQ=255)
                                                                                  aGGCATTTTTTAAAAGCACGAAAGAGGCGCACGGCGCAAAATCAATACTCCCGACAGCGACTCTGGTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGcgccg                                                       <  2:2093439/140‑1 (MQ=255)
                                                                                  aGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATCCTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGcgccg                                                       <  2:3349459/140‑1 (MQ=255)
                                                                                    gatttttttAAAAGCTCTAAATCTGCGCAAGGCGAAAAATAAATACTGCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:2908659/137‑1 (MQ=255)
                                                                                    gaaTTTTTTAAAAGCTCGAAATCTGCGAATGGCGCAAAATCACTACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  2:1001599/138‑1 (MQ=255)
                                                                                    gCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  2:3137550/140‑1 (MQ=255)
                                                                                    gCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:3500519/140‑1 (MQ=255)
                                                                                    gCATTTTTTAAAAGCTCGAAATCTGCGAAAGGCGCAAAATCAATCCTACCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  1:2149247/140‑1 (MQ=255)
                                                                                    ccATTTTTTAAAAGATCGAAATCTGCGCAAGGCGAAAAATCACTACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                     <  2:3238902/139‑1 (MQ=255)
                                                                                      aTTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATTTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTTCGGATAGCGCCGCAAg                                                   >  2:359322/1‑140 (MQ=255)
                                                                                        tttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGaa                                                 >  1:2388435/1‑140 (MQ=255)
                                                                                             aaacGCTCGAAATCTGCGCACGGCGAAAAATCAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTAc                                            <  2:683294/140‑1 (MQ=255)
                                                                                                    cGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                                     >  1:1411761/1‑140 (MQ=255)
                                                                                                    agAAATCTTCGAAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                                     <  2:1384574/139‑1 (MQ=255)
                                                                                                              gcACGGCGCAAAATCAATCCTGCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                           <  2:2579924/140‑1 (MQ=255)
                                                                                                              gcAAGGCGCAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                           <  1:1943023/140‑1 (MQ=255)
                                                                                                              gcAAGGCGAAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                           <  2:2397831/140‑1 (MQ=255)
                                                                                                              gaaaGGCGCAAAATCAATAATTCCTAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                           <  2:307182/138‑1 (MQ=255)
                                                                                                                        aactCAATACTCCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCt                 <  1:1737311/137‑1 (MQ=255)
                                                                                                                        aaaaCAACACTCCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCt                 <  2:1250778/140‑1 (MQ=255)
                                                                                                                        aaaTCACCACTCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCt                 <  1:2687149/140‑1 (MQ=255)
                                                                                                                        aaaTCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCt                 <  2:1688044/140‑1 (MQ=255)
                                                                                                                         aataaataCTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCtt                <  2:4540646/140‑1 (MQ=255)
                                                                                                                             aaTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaa            <  1:4438823/140‑1 (MQ=255)
                                                                                                                              aTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaat           >  2:1410426/1‑140 (MQ=255)
                                                                                                                                    ccGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattc     >  1:3852275/1‑140 (MQ=255)
                                                                                                                                       aaaGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattcatt  <  2:4643348/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATT  >  NZ_CP034943/2139344‑2139617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: