Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,179,645 G→T 5.8% A215S (GCC→TCC)  EO946_RS11020 → queuosine precursor transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,179,6450GT 5.8% 698.6 / 10.1 264A215S (GCC→TCC) EO946_RS11020queuosine precursor transporter
Reads supporting (aligned to +/- strand):  ref base G (119/123);  new base T (19/3);  total (138/126)
Fisher's exact test for biased strand distribution p-value = 6.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

GCAGTCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCAGA  >  NZ_CP034943/2179507‑2179783
                                                                                                                                          |                                                                                                                                          
gcagTCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGc                                                                                                                                           >  2:2088063/1‑140 (MQ=255)
   gTCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGGTTGATATTTTTATTTCTACAAATTTGATTAAGTTTGCGGTATCTTTTATTTCATTGCCTTATGCCTa                                                                                                                                        >  1:3180275/1‑140 (MQ=255)
   gTCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGGTTCATATTTTTATTTGTAAATATTTGATTAATTTTGCGGTATCTTTGATTTAATTGCCTTATGCCTa                                                                                                                                        >  1:2232666/1‑140 (MQ=255)
   gTCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTAGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTTATTAAGTTTGCGGTATCTTTGATTTCATTTCCTTATGCCTa                                                                                                                                        >  1:4979768/1‑140 (MQ=255)
    tCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAATTTTGCGGTATCTTTGATTTAATTTCCTTATGACTAt                                                                                                                                       >  2:3830561/1‑140 (MQ=255)
    tCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTAAATATTTGATTAAGTTTGCGGTATCTTTTATTTCATTGCCTTATGCCTAt                                                                                                                                       >  1:2663833/1‑140 (MQ=255)
    tCAGCCAGCTGTTTGATACATTTAGATTTACTGGTGTCGCTTTTTTCGGCCTTTATCCTGCAGGCGGTTGGCGTCATAATTTTATTTCTACATATTTGAGTAATTTTGCGGTATCTTTGATTTCATTGCGTTATGCCTAt                                                                                                                                       >  1:5225969/1‑140 (MQ=255)
      agccagcTGTTTGATACATTTCTATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGAGGTATCTTTGATTTAATTGCCTTATGCCTATgc                                                                                                                                     >  2:2702264/1‑140 (MQ=255)
      agccagcTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTGGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTTTTTGTAAATATTTTATTAAGTTTTCGGTATCTTTTTTTTCATTGCCTTATTGCTAttc                                                                                                                                     >  1:2689458/1‑138 (MQ=255)
      agccagcTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATgc                                                                                                                                     >  2:3611356/1‑140 (MQ=255)
      agccagcTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATgc                                                                                                                                     >  2:2535018/1‑140 (MQ=255)
      agccagcTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATgc                                                                                                                                     >  2:2502103/1‑140 (MQ=255)
      agccagcTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTGCATATTTTTATTTCTAAATATTTGATTAATTTTGCGGTATCGTTGATTTCATTGCCTTATGCCTATgc                                                                                                                                     >  1:1985288/1‑140 (MQ=255)
      agccagcTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCGTCAGATTTTTATTTCTACATATTTGATTAAGTTTGGGGTATCTGTGATTTAATGGCCTTATGCCTATgc                                                                                                                                     >  2:3396982/1‑140 (MQ=255)
      agccagcTGTTTGATACAGTTATATTAACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGTCGTTAGGCTTCATATTTTTATTTCTACATATTTGATTAACTTTGCGGTAGATTTGATTTCATTGACTAATTCCTAttc                                                                                                                                     >  2:5234184/1‑138 (MQ=255)
         cagcTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATgcagc                                                                                                                                  <  2:2542877/140‑1 (MQ=255)
            cTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCaaa                                                                                                                               >  2:3069487/1‑140 (MQ=255)
                 tGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGAGTAAGTTTGCGGTATCTTTGATTTAATTGCCTTATGCCTATGCAGCaaaaaaaa                                                                                                                          >  1:2482521/1‑140 (MQ=255)
                  gATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAATTTTGCGGTATCTTTGATTTAATTGCCTTATGCCTATGCAGCAAAAAAAAt                                                                                                                         >  1:2537157/1‑140 (MQ=255)
                      cATTTATATTTACTGGTGTCGCTTTTTTCTGCATTTAACCTGCAGGCGTTTGGCTTCAAATTTTTATTTATACATAATTGATTAACTTAGCGGTAACTTTGATATCATTGCCTTATGCGTATGCAGCaaaaaataatata                                                                                                                     >  2:5129426/1‑133 (MQ=255)
                      cATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATa                                                                                                                     >  2:3437608/1‑140 (MQ=255)
                      cATTTATATTTACTGGTGTCGCTTTTTTAGGCATTTATCCTGCACGCGTTTGGCTTCATATTTTTATTTCTACATATTTTATTAATGTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATa                                                                                                                     >  2:152057/1‑140 (MQ=255)
                         ttATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATTATACCg                                                                                                                  >  2:4725217/1‑140 (MQ=255)
                         ttATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCg                                                                                                                  >  2:225703/1‑140 (MQ=255)
                         ttATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTAATTTCCTTATGCCTATGCAGCAAAAAAAATGATAACg                                                                                                                  >  2:4433672/1‑140 (MQ=255)
                          tataTTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGa                                                                                                                 <  2:2961046/140‑1 (MQ=255)
                          tataTTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGa                                                                                                                 <  2:4742035/140‑1 (MQ=255)
                          tataTTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTAATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGa                                                                                                                 <  2:3895320/140‑1 (MQ=255)
                                tACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATTCCTATGCAGCAAAAAAAAATATACCGAatgatg                                                                                                           >  1:5188096/1‑140 (MQ=255)
                                 aCTGGTGTCGCTTTTTTCGGCATTTATCCTTCAGGCGGTTGGCTTGATATTTTTATTTCGACATATTTTAGTAAGTTTGCGGTATCGTTGATTTGATTTCCTTATTCGAATGCAGCAAAAAAAAAGATACCCaaataata                                                                                                          >  1:4775018/1‑134 (MQ=255)
                                 aCTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGGGTCATATTTTTATTTCTACATATTTTATTAAGTTTTCGGTATATTTTATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATTATAACGAAttatga                                                                                                          >  1:829723/1‑140 (MQ=255)
                                 aCTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAAtgatga                                                                                                          >  2:1545115/1‑140 (MQ=255)
                                 aCTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTAAATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTTCCTTATGCCTATGCAGCAAAAAAAATGATACCGAAtgatga                                                                                                          >  2:1392413/1‑140 (MQ=255)
                                 aCTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCGTCATATTTTTATTTCTACATAATTGATTAATTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAAATATACCGAAtgatga                                                                                                          >  1:541564/1‑140 (MQ=255)
                                 aCTGGTGTCGCTTTTTTCGGCATTTATACTGCAGGCGTTTGGCTTGATATTTTTATTTATACATATTTTATTAAGTTTTCGGTATATTTTATTTAATTTCCTTATTCCTATTGAGCAAAAAAAAATATAACGAattatta                                                                                                          >  1:4620492/1‑138 (MQ=255)
                                   tGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGaaa                                                                                                        <  1:1543033/140‑1 (MQ=255)
                                   tGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGaaa                                                                                                        <  1:4404524/140‑1 (MQ=255)
                                     gtgtCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTATACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAgg                                                                                                      >  2:4345371/1‑140 (MQ=255)
                                      tgtCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGa                                                                                                     >  2:1043833/1‑140 (MQ=255)
                                      tgtCGCTTTTTTCGGCATTTAGCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTTCGGTATCTTTGATTTCATTGCCTTATGCCTAGGCATCAAAAAAAATGATACCGAATGATGAAAGGa                                                                                                     >  2:2168485/1‑140 (MQ=255)
                                                 tCGGCATTTATCCTTCAGGCGTTTGTCTTAATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATATTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGc                                                                                          <  1:4289234/140‑1 (MQ=255)
                                                    gCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTa                                                                                       <  1:1811036/140‑1 (MQ=255)
                                                      aTTTATCATGCAGTCGTTTGTCTTCATATTTTTATTTCTACATATTTTATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACa                                                                                     <  1:3911548/140‑1 (MQ=255)
                                                       tttATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAg                                                                                    <  1:3968411/140‑1 (MQ=255)
                                                        ttATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGa                                                                                   <  2:749442/140‑1 (MQ=255)
                                                        ttATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGa                                                                                   <  1:2365/140‑1 (MQ=255)
                                                          ttCCTGCAGGAGTTTGTCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGTTTTCATTGCCTTATGCCTATGCAGCAAAAAAAATTATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  1:3498415/139‑1 (MQ=255)
                                                          ctCCTGCAGGCGCTTGGATTCACATATTGATTTCTACATATTTGATTAAGTTGGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  2:743975/139‑1 (MQ=255)
                                                          aTCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  2:2676742/140‑1 (MQ=255)
                                                          aTCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  2:200985/140‑1 (MQ=255)
                                                          aTCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  2:573582/140‑1 (MQ=255)
                                                          aTCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  2:470649/140‑1 (MQ=255)
                                                          aTCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  1:990201/140‑1 (MQ=255)
                                                          aTCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  1:4665037/140‑1 (MQ=255)
                                                          aTCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATAGTTGATTAAGTTTGCGGTATCTTTGAGTTCAGTGCCTAATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  2:1698115/140‑1 (MQ=255)
                                                              tGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTCTGCAGCAAACAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAataga                                                                             >  2:2704778/1‑138 (MQ=255)
                                                               gCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACATAAatatat                                                                            >  2:4835450/1‑140 (MQ=255)
                                                               gCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAatatct                                                                            >  2:4939895/1‑138 (MQ=255)
                                                               gCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAatatat                                                                            >  2:1939908/1‑140 (MQ=255)
                                                               gCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAatatat                                                                            >  2:273733/1‑140 (MQ=255)
                                                                   gCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAg                                                                        <  2:1752014/140‑1 (MQ=255)
                                                                   gCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAg                                                                        <  2:2224434/140‑1 (MQ=255)
                                                                     gTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGGAAGGAGTTAATAATGCCTACAGACATATAGGTAGAc                                                                      >  2:4572669/1‑140 (MQ=255)
                                                                     gTTGGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGAc                                                                      <  1:4229311/140‑1 (MQ=255)
                                                                        tGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGgc                                                                   >  2:861221/1‑140 (MQ=255)
                                                                              cATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAgc                                                             <  1:3830561/140‑1 (MQ=255)
                                                                              cATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAgc                                                             <  1:3443029/140‑1 (MQ=255)
                                                                              cATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAgc                                                             >  1:2733994/1‑140 (MQ=255)
                                                                               atatTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAgcg                                                            >  1:2046867/1‑140 (MQ=255)
                                                                                  tttttATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCgg                                                        <  1:662355/140‑1 (MQ=255)
                                                                                   ttttATTTCTACATATTTGATTAATTTTGCGGTATATTTGATTTCATTGCCTTATGAATATGCAGCAAAAAAAATTATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCgg                                                        <  2:3361751/140‑1 (MQ=255)
                                                                                   ttttATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCgg                                                        <  1:2088063/140‑1 (MQ=255)
                                                                                   ttttATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCgg                                                        <  2:2482521/140‑1 (MQ=255)
                                                                                   ttttATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCgg                                                        <  2:1961566/140‑1 (MQ=255)
                                                                                   ttttATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCgg                                                        <  2:3192460/140‑1 (MQ=255)
                                                                                   ttttATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCgg                                                        <  2:5166094/140‑1 (MQ=255)
                                                                                     ttATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCATATGCAGCAAAAAAAATGATAACTAATGATGAAAGGAGTTCATAATGACTACAGAAATATATGTAGACGGCGCAAGCGCAGGTa                                                      >  2:2886787/1‑140 (MQ=255)
                                                                                        tttCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATc                                                   <  1:1043833/140‑1 (MQ=255)
                                                                                         ttCTACTTATTTGATTAAGGTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAATATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATcc                                                  <  1:1282162/140‑1 (MQ=255)
                                                                                         ttCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATcc                                                  <  2:4520118/140‑1 (MQ=255)
                                                                                            tACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCgg                                               >  1:3796923/1‑140 (MQ=255)
                                                                                             aCATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGa                                              <  2:325187/140‑1 (MQ=255)
                                                                                             aCATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGa                                              <  1:2502103/140‑1 (MQ=255)
                                                                                                  tttGATTAATTTTGCGTTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATc                                         <  1:5146245/140‑1 (MQ=255)
                                                                                                               gCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCAtt                            <  1:3069487/140‑1 (MQ=255)
                                                                                                                     tCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATc                      <  2:1652382/140‑1 (MQ=255)
                                                                                                                     tCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATc                      >  1:4452181/1‑140 (MQ=255)
                                                                                                                     tCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATc                      >  1:3532863/1‑140 (MQ=255)
                                                                                                                     tCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATc                      >  1:156945/1‑140 (MQ=255)
                                                                                                                          gATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACa                 >  2:1472170/1‑140 (MQ=255)
                                                                                                                               cATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 >  1:3189319/1‑71 (MQ=255)
                                                                                                                               cATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGaaa                                                                                 <  2:3189247/71‑1 (MQ=255)
                                                                                                                               cATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAg            >  2:1774056/1‑140 (MQ=255)
                                                                                                                               cATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAg            >  1:2418941/1‑140 (MQ=255)
                                                                                                                                       tATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCa    <  2:3496216/140‑1 (MQ=255)
                                                                                                                                       tATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCa    >  2:401353/1‑140 (MQ=255)
                                                                                                                                       tATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCa    <  1:3699524/140‑1 (MQ=255)
                                                                                                                                       tATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCa    <  1:2323924/140‑1 (MQ=255)
                                                                                                                                       tATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAACCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCa    <  2:115795/140‑1 (MQ=255)
                                                                                                                                         tGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACATAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCAGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCAGa  >  2:422506/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GCAGTCAGCCAGCTGTTTGATACATTTATATTTACTGGTGTCGCTTTTTTCGGCATTTATCCTGCAGGCGTTTGGCTTCATATTTTTATTTCTACATATTTGATTAAGTTTGCGGTATCTTTGATTTCATTGCCTTATGCCTATGCAGCAAAAAAAATGATACCGAATGATGAAAGGAGTTCATAATGCCTACAGAAATATATGTAGACGGCGCAAGCGCCGGTAATCCCGGACTATCCGGGATCGGCATTTTTATCAAACATGAAGGAAAAGCAGA  >  NZ_CP034943/2179507‑2179783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: