BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,528	G→T	6.6%	Q57K (CAA→AAA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,528	0	G	T	6.6%	893.7 / 11.4	363	Q57K (CAA→AAA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base G (70/252); new base T (29/9); total (99/264)
Fisher's exact test for biased strand distribution p-value = 3.71e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

CCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCT  >  NZ_CP034943/2214392‑2214664

  gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                                     <  1:3495373/140‑1 (MQ=255)

    aaTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaataat                                                                                                                                   <  2:3687201/140‑1 (MQ=255)

     aTCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATg                                                                                                                                  >  2:2401379/1‑140 (MQ=255)

        ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGttt                                                                                                                               >  1:448017/1‑140 (MQ=255)

        ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGttt                                                                                                                               >  1:837804/1‑140 (MQ=255)

         tCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGtttt                                                                                                                              <  2:5196570/140‑1 (MQ=255)

             taatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTc                                                                                                                          <  2:5025416/140‑1 (MQ=255)

              aatCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCa                                                                                                                         <  1:5046362/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGGTTTATTACGATACAAACCGATTGATTTAAGATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCAct                                                                                                                       <  2:1833588/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATTGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  1:1626557/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  2:325252/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  1:2164581/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  1:62066/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  1:2401379/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  1:2512100/140‑1 (MQ=255)

                tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  1:1693115/140‑1 (MQ=255)

                tCAGTTTGCTCAGTTTGTGGATGTATTACGCTTTAGTACGATACAAACCGATTGATTTAATATCCTGCTGAATCTCTTCCAGCGAAACAGCCAAATAGTCGTCCGGCCGTTTATATTTGTCAAATAATGTTTTGGTCAct                                                                                                                       <  1:4410325/140‑1 (MQ=255)

                acATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCAct                                                                                                                       <  2:1231859/139‑1 (MQ=255)

                  aTTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACACACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGACGTTGATATTTTTGAAATAATGTTTTGGTAActct                                                                                                                     >  2:1129657/1‑140 (MQ=255)

                  aTTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGGCGTTTATATTTGTGAAATAATGTTTTTGTCActct                                                                                                                     >  2:1095794/1‑140 (MQ=255)

                  aTTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTTGTCActct                                                                                                                     >  2:3127482/1‑140 (MQ=255)

                        cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAc                                                                                                               <  1:1008514/140‑1 (MQ=255)

                           cgTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  2:2296807/139‑1 (MQ=255)

                           cgTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  2:4605238/139‑1 (MQ=255)

                           aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  1:1468407/140‑1 (MQ=255)

                           aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  1:3855204/140‑1 (MQ=255)

                           aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  2:2475306/140‑1 (MQ=255)

                           aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  1:456465/140‑1 (MQ=255)

                           aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAACCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  1:4766978/140‑1 (MQ=255)

                           aGTGTTTGTATGTTTGTCGCTGTCTTACGATACAAACCGATTGATTTCATATCCTGCGGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATCTGTTTGAACTAATGTTTTGGTCACTCTGTTTACAAg                                                                                                            <  2:3793218/140‑1 (MQ=255)

                             tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                          <  1:3754238/140‑1 (MQ=255)

                             tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                          <  1:40246/140‑1 (MQ=255)

                             tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                          <  1:4064138/140‑1 (MQ=255)

                             tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCGGTTTACAAGTg                                                                                                          <  2:402841/140‑1 (MQ=255)

                             tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                          <  2:2837026/140‑1 (MQ=255)

                             attttGGATGTTTGTCGCTTTATTACGATACAAAACGATTGATTTAATATCCTGATGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCTGACGTTTATAATTTTGAAATAATGTTTTGCTCACTCTGTTTACAAGTg                                                                                                          <  2:3862775/139‑1 (MQ=255)

                                 tGGATGTTTTTCGCTTTAGTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATc                                                                                                      <  2:4461912/140‑1 (MQ=255)

                                      gTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTAc                                                                                                 <  1:3947094/140‑1 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTTCATATGTaaa                                                                                                >  1:4122552/1‑138 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAAAAATGTTTTTGTCACACTGTTTAAAAATTAATCTGTaaa                                                                                                >  1:3161128/1‑138 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                <  2:3094477/140‑1 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                <  1:4332032/140‑1 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACa                                                                                                >  2:3533352/1‑140 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTAAATTTTTGAAATAATGTTTTTGTCACTCTGTTTACAAGTGCAtatttaaa                                                                                                >  2:2440944/1‑133 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTTAAAAATTTTTTGGTCACTATGTTTAAAAGTTGATCTTTACa                                                                                                >  1:310344/1‑140 (MQ=255)

                                       tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACACTGTTTAAAAATGCATATGTaaa                                                                                                >  1:5235699/1‑138 (MQ=255)

                                            cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTGTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                           <  1:359044/140‑1 (MQ=255)

                                            cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGATGAATCTCTTTCATCGAAACAGCCAAATAGTGTTCCGGACGTTTATATTTTTGAAATAATGTTTTGGTCCCTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                           <  1:3007654/140‑1 (MQ=255)

                                            cGCTGTAGTACGATACAAACCGATTGATTTAATATCCTGCGGAAGCTCTTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGAGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                           <  1:3301712/140‑1 (MQ=255)

                                                         tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTATTTTTTTAATAATTTTTTTGTGACTCTGTTTTAAATTTCATCTGTACATTGCGCAGATAAAGCGAc                                                                              >  1:98269/1‑140 (MQ=255)

                                                         tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAAAAATTTTTTTGTGAATCTTTTTTAAAGTGGATCTGTACATTTCGCAGATAAAACGAc                                                                              >  1:2267017/1‑140 (MQ=255)

                                                         tACAAACCGAGTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCACATAGTCTTCCGGCCGTGTATATTTTTTAAATTAGGTTTTGGTGACTCTGTTTACAATTGCATCTGTACAATGCGCAGATAAAGCGAc                                                                              >  1:4773102/1‑140 (MQ=255)

                                                              aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTa                                                                         >  2:2350777/1‑140 (MQ=255)

                                                              aCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTATGTTTAAAAGTGCATCTGTACATTTCGCAGATAAAACGACTGCTa                                                                         >  2:4860211/1‑140 (MQ=255)

                                                                 gattgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACTGCTAACa                                                                      >  2:2083724/1‑140 (MQ=255)

                                                                  attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCAc                                                                     <  2:2218722/140‑1 (MQ=255)

                                                                  attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTAAAATTTTGAAATAATGTTTTTGTCACAAGGTTTAAAAGATCATCAGTAAAATTCGCAGATAAAGCGAAAGCAACCaa                                                                     >  2:1677870/1‑139 (MQ=255)

                                                                          aaTATCCTGCTGAAGCTCTTCCAGCTAAACAGCCAAATAGTCTTCCGCCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCaa                                                             <  1:1035395/140‑1 (MQ=255)

                                                                           atatCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaaa                                                            <  1:2350777/140‑1 (MQ=255)

                                                                               ccTGCTGAAGCTCGTCCCGCGAACCAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTCCATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGa                                                        <  2:4982953/140‑1 (MQ=255)

                                                                                ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                       <  1:514318/140‑1 (MQ=255)

                                                                                ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCAGTTTATATTTTTTAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                       <  1:2005153/140‑1 (MQ=255)

                                                                                aggctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                       <  2:4909933/138‑1 (MQ=255)

                                                                                 tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTATTTTTGTAATAATGTTTTTGTGACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAACGACTGCTACCACAAAATCAAAAGGAtt                                                      >  1:1225009/1‑140 (MQ=255)

                                                                                 tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAAATTTTTTGTGACTCTGTTTAAAATTGCATCTGTAAATTGCGCAGATAAATCGACAGCTACCACGAAATAAAAAGGAtt                                                      >  1:1106421/1‑140 (MQ=255)

                                                                                 tgctgAAGCTCTTCCAGCGAAACAGCCAAAGAGTCTTCCGGCCGTGTATATTTTTTTAATAAAGTTTTGGTGACTCGGTTTAAAAGTGCATCTGTACCAGTCGCAGATAAAGGGACAGCTACCACAAATTCAAAAGGCtt                                                      >  1:706183/1‑140 (MQ=255)

                                                                                         ctctTCCAGCGCCACAGCCACAGAGTCTTCCGGCCGTTTATATTTTGCAAATAATGTTTTGGTCACTCTGTTCACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                              <  1:4860211/140‑1 (MQ=255)

                                                                                         ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCTTTTATTTTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                              <  2:2161503/140‑1 (MQ=255)

                                                                                         ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                              <  2:2545398/140‑1 (MQ=255)

                                                                                         ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                              <  1:2615052/140‑1 (MQ=255)

                                                                                         ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                              <  1:3670316/140‑1 (MQ=255)

                                                                                         ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                              <  2:1619806/140‑1 (MQ=255)

                                                                                           ctTCCAGCTAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAATTATTGTTTTGGTCTCTGTGTTTACACGTGCATCTGTACATTGCGCACATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                            <  2:4741484/140‑1 (MQ=255)

                                                                                           ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                            <  1:2934797/140‑1 (MQ=255)

                                                                                           ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGGACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                            <  2:63372/140‑1 (MQ=255)

                                                                                           ctTCCAGCGAAACAGCCAAATAGTATTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTAAATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                            <  2:4511337/140‑1 (MQ=255)

                                                                                             tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTATTTTTTTAATAATGTTTTGGTGACGCTGTTTTCAAGTGCAGCTGTTCAGTGCGCCGATAAACCGACGGCGACCACAAAATAAAAAAGATTTGAATGAAcccg                                          >  1:3544382/1‑138 (MQ=255)

                                                                                             tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                            >  2:3618145/1‑138 (MQ=255)

                                                                                             tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTGAATAAAGTTTTGGTCACTCTGGTTAAAAGTGCATCTGTACATTTCGCAGATAAATCGACTGGTAACACAAATTCAAAAGGATTTGAATTaaacct                                          >  1:4909933/1‑136 (MQ=255)

                                                                                             tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAATGTTTTGGTCACTCTGTTTTCAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAg                                          >  2:393303/1‑140 (MQ=255)

                                                                                             tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGTAATAATGTTTTGGTCACGCTGTTTTCAAGTGCATCTTTACATTGCGCAGATAAAGCGACTGCTAACACAAATTCAAAAGGATTTGAATTAACCAg                                          >  2:3298625/1‑140 (MQ=255)

                                                                                             tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACAAATTAAAAAGGCTTGGAATGAAcccg                                          >  2:87814/1‑138 (MQ=255)

                                                                                              ccAGAGAAACAGACAAATAGTCTTCCGGCCTTTTATATTTTTTAATTAATGTTTTTGTCACTCTGTTTACAAGTGCATCGGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGt                                         <  1:4628337/140‑1 (MQ=255)

                                                                                               cAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                        <  2:4121385/140‑1 (MQ=255)

                                                                                                 gCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCCGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                                      <  1:2234544/140‑1 (MQ=255)

                                                                                                  cGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTGACTGTGTTTACAAGTTCATCTGTACAATGCGCAGATAAAGCCACTGCTACCACAAATTAAAAATGATTTGAATGAACCACTTcaa                                     >  1:3776324/1‑139 (MQ=255)

                                                                                                      aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACCGCTACCAc                                                                     <  1:2788817/104‑1 (MQ=255)

                                                                                                      aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATATGTACATTGCGCAGATAAAGCGACCGCTACCAc                                                                     >  2:2788817/1‑104 (MQ=255)

                                                                                                         gCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGc                              <  2:603087/140‑1 (MQ=255)

                                                                                                                    ttCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCACTGGGTTTACAAGTGCATCTGTAACTTGCGCAGATAAAGCGACTGCTACCACAAATTCAAAAGGATTGGAATTTACCAGTTAACAATCTGCGGTTGGAAACa                   >  2:3015931/1‑140 (MQ=255)

                                                                                                                    ttCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTGACTGTGTTTACAAGTGCATCTGTAAATTGCGCAGATAAATCCACTGCTACCACAAAATCAAAAGGATTGGAATGAAACAGTTAACACGCTGCCTGTGGAAACa                   >  2:2464329/1‑140 (MQ=255)

                                                                                                                          ccGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc             <  2:4825158/140‑1 (MQ=255)

                                                                                                                              ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt         <  2:1399107/140‑1 (MQ=255)

                                                                                                                              ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt         <  2:1990564/140‑1 (MQ=255)

                                                                                                                              ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt         <  1:917761/140‑1 (MQ=255)

                                                                                                                              ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt         <  1:1331728/140‑1 (MQ=255)

                                                                                                                              ttATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGCGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt         <  1:3360149/140‑1 (MQ=255)

                                                                                                                              ttATATTTTTGAAATAATGTTTTGGTAACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt         <  1:4938428/140‑1 (MQ=255)

                                                                                                                               tataTTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATc        <  1:189713/140‑1 (MQ=255)

                                                                                                                                     tttGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCt  <  2:2722753/140‑1 (MQ=255)

CCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCT  >  NZ_CP034943/2214392‑2214664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`