Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 44,881 G→A 76.7% intergenic (‑62/‑439) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494344,8810GA76.7% 331.1 / 103.3 169intergenic (‑62/‑439)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (16/23);  new base A (60/69);  total (77/92)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

AGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTT  >  NZ_CP034943/44745‑45009
                                                                                                                                        |                                                                                                                                
agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                               <  2:1112167/140‑1 (MQ=255)
agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                               <  1:2805091/140‑1 (MQ=255)
agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                               <  1:2920829/140‑1 (MQ=255)
agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                               <  2:3598634/140‑1 (MQ=255)
agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACa                                                                                                                               <  2:2515735/140‑1 (MQ=255)
 gTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTcaacaa                                                                                                                              <  1:1463565/140‑1 (MQ=255)
 gTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACaa                                                                                                                              <  2:1270663/140‑1 (MQ=255)
   acgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACaaaa                                                                                                                            >  1:1933635/1‑140 (MQ=255)
       cgCAATTCGATAGGAATAACTACACGTCCTAGCTCAACAACTTTACGTACACTACCCGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                        <  1:1077863/140‑1 (MQ=255)
        gaaaTTAGATAGGAATAACTACCAGTCCTAGCTCATCAACTTTACGTACAATAACAGTAGATTTCATAAACTTTCTCCTACCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCtt                                                                                                                       <  2:3474160/138‑1 (MQ=255)
        gCAATTCGATAGGACTAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCCACAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                                       <  2:1142330/140‑1 (MQ=255)
        gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                                       <  1:998992/140‑1 (MQ=255)
        gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACGTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                                       <  1:5200887/140‑1 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                     >  1:4115949/1‑140 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                     <  1:1777872/140‑1 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                     <  1:3692386/140‑1 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTAc                                                                                                                     >  1:3334217/1‑140 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTAc                                                                                                                     >  1:5230790/1‑140 (MQ=255)
               gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCa                                                                                                                <  2:2074132/140‑1 (MQ=255)
               gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                                <  2:4686257/140‑1 (MQ=255)
               gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCa                                                                                                                <  2:2247190/140‑1 (MQ=255)
                    gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                        <  2:1933964/127‑1 (MQ=255)
                    gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCa                                                                                                           >  1:1433192/1‑140 (MQ=255)
                        aaCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                                       <  1:2506608/140‑1 (MQ=255)
                        aaCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                                       <  2:3287539/140‑1 (MQ=255)
                             cacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGt                                                                                                  >  2:804289/1‑140 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                                       >  2:1662517/1‑134 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                                       >  2:1835266/1‑134 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                                       >  2:360996/1‑134 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAAc                                                                                                       <  1:4274950/134‑1 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                 >  2:1567172/1‑140 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                 >  1:5075648/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCTACAAAATCCTTACCTTCATAGCATTAGAAGGttt                                                                                                >  2:2334116/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:613685/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  1:606031/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  1:4560116/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  1:166610/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  1:1539520/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:3884869/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:1319488/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTAATAGCATAACAAGGttt                                                                                                >  2:3703990/1‑140 (MQ=255)
                                  ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                             >  1:3538335/1‑140 (MQ=255)
                                  ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                             >  2:4401469/1‑140 (MQ=255)
                                  ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTACa                                                                                             >  2:4264438/1‑140 (MQ=255)
                                    tAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaat                                                                                           <  2:2447047/140‑1 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCGTAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                     >  2:3487153/1‑140 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATATCATAACAAGGTTTCCAATAATCGt                                                                                     >  2:3161953/1‑140 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                     >  1:1791522/1‑140 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                     >  1:2225672/1‑140 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                     <  1:5000534/140‑1 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                     <  2:4350304/140‑1 (MQ=255)
                                            caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                                   >  1:1694004/1‑140 (MQ=255)
                                                  tACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtt                                                                             >  2:659982/1‑140 (MQ=255)
                                                      tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCGTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                         <  1:3154060/140‑1 (MQ=255)
                                                       aaaaTACCAGTAGATTTCATAAACGTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                                        <  2:1943459/138‑1 (MQ=255)
                                                       aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                                        <  2:4115949/140‑1 (MQ=255)
                                                       aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                                        <  2:2863015/140‑1 (MQ=255)
                                                       aCAATACCAGTAGATTTCATAAACGTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                                        <  2:385481/140‑1 (MQ=255)
                                                                gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTTTTTGCTattga                                                               >  1:732383/1‑140 (MQ=255)
                                                                gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                               <  1:1634776/140‑1 (MQ=255)
                                                                gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                               >  1:2057472/1‑140 (MQ=255)
                                                                gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                               >  1:3742013/1‑140 (MQ=255)
                                                                   gATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAGCAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgattg                                                            >  1:2720302/1‑140 (MQ=255)
                                                                    aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                                           <  2:1539520/140‑1 (MQ=255)
                                                                    aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                                           <  2:166610/140‑1 (MQ=255)
                                                                    aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                                           <  2:1540472/140‑1 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          <  1:3487359/140‑1 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTACTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          <  2:2529416/140‑1 (MQ=255)
                                                                     tttCATAAACTGTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          <  1:1912544/140‑1 (MQ=255)
                                                                     tttCATAAACGTTCTCCTCCCAAGAGATGATGATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACCAAATCCTTACCTTCCTAGCATAACAAGGTTTCCAATCATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          <  1:4661428/140‑1 (MQ=255)
                                                                      ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                                         >  1:251526/1‑140 (MQ=255)
                                                                        cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgatt                                                             >  1:1978320/1‑134 (MQ=255)
                                                                        cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGa                                                       >  1:2472591/1‑140 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  2:460171/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  1:613685/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  2:2422283/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  2:2354065/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  2:3558237/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAGCAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                  <  2:2720302/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                  <  1:2011702/140‑1 (MQ=255)
                                                                                    cctccCAAGAGAGGATTATTTGTTTATATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGGATAACCAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                           <  2:4852398/140‑1 (MQ=255)
                                                                                       ccccAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                        <  2:902516/140‑1 (MQ=255)
                                                                                       cccAACAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                        <  1:3640444/140‑1 (MQ=255)
                                                                                        ccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTc                                       <  2:4822074/140‑1 (MQ=255)
                                                                                          aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCCGAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                                     <  1:3441038/140‑1 (MQ=255)
                                                                                          aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                                     <  1:2979228/140‑1 (MQ=255)
                                                                                            gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                                   <  1:3603104/140‑1 (MQ=255)
                                                                                            gagaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                                   <  1:1604141/140‑1 (MQ=255)
                                                                                              gatgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                                 <  1:4834279/140‑1 (MQ=255)
                                                                                              gatgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTAGAGTCAGTAAt                                 <  2:104633/140‑1 (MQ=255)
                                                                                               atgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                                >  1:978130/1‑140 (MQ=255)
                                                                                               atgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                                <  1:3189783/140‑1 (MQ=255)
                                                                                               atgatTATTTGTTTAAATTATATTTTTCTACGTCATTCTTCAACAAAATCCTTACCTTCATCGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                                <  1:3105371/140‑1 (MQ=255)
                                                                                               atgatTATTTGTTTAAATTATATTGTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                                <  1:1220926/140‑1 (MQ=255)
                                                                                                 gatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTa                              >  2:2985041/1‑140 (MQ=255)
                                                                                                       ttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaa                        >  2:4110512/1‑140 (MQ=255)
                                                                                                        tGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaaa                       <  2:5075648/140‑1 (MQ=255)
                                                                                                                    atTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                                   >  2:1614623/1‑116 (MQ=255)
                                                                                                                             tctCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGaaa                       >  1:877196/3‑119 (MQ=255)
                                                                                                                             tctCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCttttt  >  2:574010/3‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                
AGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTT  >  NZ_CP034943/44745‑45009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: