Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,269,970 G→T 6.1% S70Y (TCT→TAT)  prsW ← glutamic‑type intramembrane protease PrsW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,269,9700GT 6.1% 933.4 / 3.6 370S70Y (TCT→TAT) prsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base G (191/120);  new base T (3/38);  total (194/176)
Fisher's exact test for biased strand distribution p-value = 1.28e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

AATATTTTCGAGGGTTGCAAAACCGAGCGATACACTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTA  >  NZ_CP034943/2269831‑2270101
                                                                                                                                           |                                                                                                                                    
       tCGAGGGTTGCAAAACCGAGCGATACACTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATaaaaa                                                                                                                               <  1:2396461/140‑1 (MQ=255)
          aGGGTTGCAAAACCGAGCGATACACTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGa                                                                                                                            >  2:813786/1‑140 (MQ=255)
                                cacTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTccc                                                                                                      >  1:3406448/1‑140 (MQ=255)
                                cacTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTccc                                                                                                      >  1:1936327/1‑140 (MQ=255)
                                cacTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACAATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTccc                                                                                                      >  1:3114173/1‑140 (MQ=255)
                                   ttGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCcaca                                                                                                   >  2:3278618/1‑140 (MQ=255)
                                   ttGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCcaca                                                                                                   >  2:1966555/1‑140 (MQ=255)
                                   ttGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCcaca                                                                                                   >  2:2242255/1‑140 (MQ=255)
                                     gCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACAtt                                                                                                 >  2:284072/1‑140 (MQ=255)
                                     gCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACAtt                                                                                                 >  1:3703135/1‑140 (MQ=255)
                                        ccGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATtctc                                                                                              >  2:4118915/1‑140 (MQ=255)
                                        ccGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATtctc                                                                                              >  2:1770141/1‑140 (MQ=255)
                                        ccGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATtctc                                                                                              >  2:2487732/1‑140 (MQ=255)
                                           tataCAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAAAACTGACCATCAGAATAAACCATTTAAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACAGTCTCttt                                                                                           >  2:1455454/1‑140 (MQ=255)
                                                aaTCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCaa                                                                                      >  2:730158/1‑140 (MQ=255)
                                                 aTCCCCTCATAATGCTCAGCAAAGTGGGCGGGCGGGTAAAGACTGACCATCAGAATAAACCATTGTAATGATTCCTCCAACAACCCTGACCAGAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAg                                                                                     <  2:501654/140‑1 (MQ=255)
                                                     cGTCCTAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGTAGAGAAACAAGAAACGAAAAAGCTTCCGCCTCCCACAGTCTCTTTTTCAAGCACg                                                                                 >  1:2497940/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCCTCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  2:2308325/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATACACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  2:3701908/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATtc                                                                                                >  1:2069328/1‑125 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATtc                                                                                                <  2:2069328/125‑1 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  2:4423122/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  1:3081913/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  2:1831235/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  1:1792576/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  1:1880355/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACAGTCTCTTTTTCAAGCACg                                                                                 >  1:2696951/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGATTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                 >  2:4702474/1‑140 (MQ=255)
                                                     cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAAGAAACCATTTTAATGATTCCTCCAAAAACACTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCCAGCACg                                                                                 >  2:861015/1‑140 (MQ=255)
                                                       taaTAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACAATCAGAATAAACCATTTTAATCATACCTCCAAAAACCCTCAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTa                                                                               <  1:73180/138‑1 (MQ=255)
                                                       tCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTa                                                                               >  1:1126951/1‑140 (MQ=255)
                                                        cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              <  1:4722431/140‑1 (MQ=255)
                                                        cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              <  1:2973460/140‑1 (MQ=255)
                                                        cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              <  1:5087395/140‑1 (MQ=255)
                                                        cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              <  2:4850055/140‑1 (MQ=255)
                                                        cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              <  2:3583187/140‑1 (MQ=255)
                                                        cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACAATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              <  2:4450401/140‑1 (MQ=255)
                                                        cATAATGCTAATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              <  2:3127225/140‑1 (MQ=255)
                                                                tcatcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATaaa                                                                      <  2:1112460/140‑1 (MQ=255)
                                                                 catcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAATAGTAACGAAAAAGCTTCCGCCTCCCACCTTCTCTTTTTCAAGCACGTACTGGATAAAc                                                                     >  1:4110489/1‑140 (MQ=255)
                                                                 catcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAAc                                                                     >  1:2352050/1‑140 (MQ=255)
                                                                 catcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAAc                                                                     >  1:961206/1‑140 (MQ=255)
                                                                 catcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTATCTTTTTCAAGCACGTACTGGATAAAc                                                                     >  2:2368846/1‑140 (MQ=255)
                                                                  atcaAAGTGGGCGTGCGGGTAAACACTGACCATCCGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGATTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACa                                                                    >  2:240174/1‑140 (MQ=255)
                                                                  atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGc                                                                                    >  1:1235681/1‑124 (MQ=255)
                                                                  atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGCTaaaaa                                                                    >  2:4067094/1‑138 (MQ=255)
                                                                  atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACAGTCTCTTTTTCAAGCACGACCTGGATAAACa                                                                    >  1:243088/1‑140 (MQ=255)
                                                                  atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGAGTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGc                                                                                    <  2:1235681/124‑1 (MQ=255)
                                                                       aGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATtgtg                                                               <  2:3216380/140‑1 (MQ=255)
                                                                       aGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATtgtg                                                               <  2:3059247/140‑1 (MQ=255)
                                                                         tGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTggg                                                            <  2:191443/140‑1 (MQ=255)
                                                                          gggCGTGCGTGTAAAAACTGACAATCAGAATAAACAATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCGTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGa                                                           <  1:1409747/140‑1 (MQ=255)
                                                                          gggCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGACAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGa                                                           <  2:4565336/140‑1 (MQ=255)
                                                                          gggCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGa                                                           <  2:4754926/140‑1 (MQ=255)
                                                                            gCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCa                                                                                       >  1:3218036/1‑111 (MQ=255)
                                                                            gCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCa                                                                                       <  2:645553/111‑1 (MQ=255)
                                                                            gCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGaaa                                                         >  2:1408226/1‑140 (MQ=255)
                                                                            gCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAACTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                              >  1:5221600/1‑120 (MQ=255)
                                                                                 cGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACaa                                                    >  1:4451033/1‑140 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAATGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTGATGCACGTCCTGGATAAACATTGTGGGAAATACAAATACAACACCTAg                                       >  2:3184218/1‑140 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTCCTGGATAAACATTGTGTGAAATACAAGAACAACCCCtcg                                       >  1:2778700/1‑138 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGCAATACAAGAACAACGCCTAg                                       >  1:3509574/1‑140 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                >  2:3510869/1‑131 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                >  2:170367/1‑131 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                       >  1:946922/1‑140 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                       >  2:2720187/1‑140 (MQ=255)
                                                                                              aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCACCTCCCACATTCTCTTTTTCAAGCACGTa                                                                               <  2:3529217/101‑1 (MQ=255)
                                                                                                   cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                  >  1:2199303/1‑140 (MQ=255)
                                                                                                   cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                  >  2:4028103/1‑140 (MQ=255)
                                                                                                   cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                  >  2:106266/1‑140 (MQ=255)
                                                                                                   cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                  >  2:955459/1‑140 (MQ=255)
                                                                                                   cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                  >  2:214891/1‑140 (MQ=255)
                                                                                                     gattAAATCATTTTAATTATTAGTCCAAAAACCGTGAACTTAATAATCAACCTAAAAAGCTTCCGCCTCCCAATTTGTCTTTTTCAACCAGGTACTGTATATACTTTGTGTGAATTACCATAAAACCCCCTAGAAAAAAc                                <  2:2232294/137‑1 (MQ=255)
                                                                                                        tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTCCTGGATAAACATTGTGGGAAATACAAGAACCACCCCTAGAAAAAACGAc                             >  1:1555632/1‑140 (MQ=255)
                                                                                                             aaTTTTATTTATTCCTCCAAAAACCCTCAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTATTTTTAAAGCACGTACTGTATAAACATTGTGGTAAATACAAGAACAACCCCTATAAAAAACGACCGAAg                        <  2:1621132/139‑1 (MQ=255)
                                                                                                              aTTTTATTGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTAGTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                       <  2:3380510/140‑1 (MQ=255)
                                                                                                              aTTTTATTGATTCCTCCAAAAACCCTGAACATAAAAAACAAACTAAAAAGCGTCCGCCTCCAACATTCTCTTTTTAAAGCACGTACTGTATAAACATTGTGGTAAAAACAAGAACAACCCCTAGAAAAAACGACCGAAGc                       <  2:3325994/140‑1 (MQ=255)
                                                                                                              aTTTTAATTATACCACCAAAAACCCTCAATAAAAAAAAAAAACCAAAAAGCTTCCCCCCCCAAATTTCTTTTTTTCAAGCACGTACTGGATAAACATTGTGGTAAATACAACAACAACCCCTCCAAAAAACGACCGAAGc                       <  1:2938092/140‑1 (MQ=255)
                                                                                                              aTTTTAATGTTTCCTCCAAAAACCCTGAAGATAAAAAAAAAACTAAAAAGCTTCCGCCTCCCACATTATATTTTACAAGCACGTACTGCATAAACATTGTGGTAAATACAACAACAACCCCTAAAAAAAACGACCGAAGc                       <  1:2638131/140‑1 (MQ=255)
                                                                                                              aTTTTAATGATTCCTACAAAACCCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                       <  2:1302310/140‑1 (MQ=255)
                                                                                                              aTTTTAATGATTCATCAAAAATCCCTGAATATAAAAAAGAAACGAAAAAGATTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAATAAAAACCCCTAGAAAAAACGACCGAAGc                       <  2:2516319/140‑1 (MQ=255)
                                                                                                                tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                     >  1:3764936/1‑140 (MQ=255)
                                                                                                                tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                     >  1:3866900/1‑140 (MQ=255)
                                                                                                                 ttAATTATTCCTCCAAAAACCCTTAAGATAAAAAACAAACCAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCAcc                    <  1:847678/140‑1 (MQ=255)
                                                                                                                 ttAATGTTTCCTCAAAAAACCCTCAATAAAAAAAACAAACCAAAAAGCTACCGCCACCAACATTCTATTTTTAAAGCACGTACTTTATAAACTTTGTGTCAAATACAACAACACCCCCTAGAAAAAACGACCGAAGCAcc                    <  1:1701955/140‑1 (MQ=255)
                                                                                                                 ttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAATAACAACCCCTAGAAAACACGACCGAAGCAcc                    <  2:520835/140‑1 (MQ=255)
                                                                                                                 ttAATGAGTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTATCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAcc                    <  2:3645135/140‑1 (MQ=255)
                                                                                                                  tAATGATACCACAAAAAACCCTTAAGAAAAAAAACAAACCAAAACGCTTCCGCCCCCAACATTCTATTTTTCACGCACGTCCTGTATAAACTTTGTGGCAAATAAAACAACAACCCCTACAAAAAACGACCGAAGCACCa                   <  1:4255805/140‑1 (MQ=255)
                                                                                                                   attgtttCATCCAAAAACCCTTACTATAAAAAATAAACTAAAAAGGTTCCGCCTCCAAATTTATCTTTTTAAAGCACGTACTGTATAAACATTTTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                  <  2:3392122/135‑1 (MQ=255)
                                                                                                                     tGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCagat                >  2:977121/1‑140 (MQ=255)
                                                                                                                         tcctcaAAAATCCCTGTATATATAAAATAAAGTATAATGCTTCCGCCTCCGATATTATTTTTTTAAAGCACGTACTGTATTAACATTGTGGGAAATACAAGAACAACCCCTAGATAAAACGACCGAAGCACCATATGTAc            <  2:3786214/140‑1 (MQ=255)
                                                                                                                            cccAAAAACCATCAACATAAAAAACAAACCAAAACCCTTCCCCCGCCCACATTCTCTTTTTCAAGCACGTACTGCAAAAACATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAgg         <  2:437871/139‑1 (MQ=255)
                                                                                                                              cAAAAACCCTGAACATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAATGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt       <  2:4218454/140‑1 (MQ=255)
                                                                                                                              cAAAAACCCTCACCATAAAAAACAAACCAAAAAGCTTCCGCCACCCACATACTCTTTTTCACCCACGTACTGCATAAACATTGTGGCAAATACAACACCAACCCCAACAAAAAACGACCGAAGCACCATATGTACAGGtt       <  1:4835424/140‑1 (MQ=255)
                                                                                                                              aaaaaaCCCTTAATATAAAATAAAAACTAAAAAGCTGCCGCCACCCAAATTCTATTTTTAAAGCACTTAGTGTATAAACTTTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt       <  1:4395093/139‑1 (MQ=255)
                                                                                                                              aaaaaaCCCTTAACAAAAAAAACAAACCAAAACGCGCCCCCCTCCCACTTTCTCTTTTTCAAGCACGTACTGTATAAACTTTGTGGTAAATACACCAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt       <  1:106266/139‑1 (MQ=255)
                                                                                                                              aaaaaaCCCTCAATATAAAAAACAAACCAAAAAGCTCCCGCCACCCAAATTCTCTTTTTCACGCACGTACTGTATAAAAATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt       <  1:3622277/139‑1 (MQ=255)
                                                                                                                              aaaaaaCAATAAACATAAAAAAAAAACCAAAAAGATACCGCCTCCCAAATTATCTTTTTAAAGCACGTACTGTATAAACATTGTGTGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt       <  2:4639310/139‑1 (MQ=255)
                                                                                                                                aaacCCCTCAATATAAAAAACAAACCAAACAGCTCCCGCCACCAACATTCTATTTTTCACGCACGTAGTGTATAAAATTGGGGCGAAATACAACACCACCCCACACAAAAAACGACCGAAGCACCATATGTACAGGTTCa     <  1:596752/140‑1 (MQ=255)
                                                                                                                                aaaaCCCTTAATATAAAAAACAAACTAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAACAATTGTGGCAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCa     <  2:1585963/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                    
AATATTTTCGAGGGTTGCAAAACCGAGCGATACACTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTA  >  NZ_CP034943/2269831‑2270101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: