Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,269,984 G→C 7.5% F65L (TTC→TTG prsW ← glutamic‑type intramembrane protease PrsW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,269,9840GC 7.5% 936.4 / 7.8 369F65L (TTC→TTGprsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base G (185/123);  new base C (2/32);  total (187/182)
Fisher's exact test for biased strand distribution p-value = 5.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

TTGCAAAACCGAGCGATACACTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAA  >  NZ_CP034943/2269845‑2270123
                                                                                                                                           |                                                                                                                                           
ttGCAAAACCGAGCGATACACTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACg                                                                                                                                             >  2:4012899/1‑140 (MQ=255)
                  cacTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTccc                                                                                                                           >  1:3436830/1‑140 (MQ=255)
                  cacTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTccc                                                                                                                           >  1:1936327/1‑140 (MQ=255)
                     ttGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCcaca                                                                                                                        >  2:1966555/1‑140 (MQ=255)
                       gCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACAtt                                                                                                                      >  1:828979/1‑140 (MQ=255)
                       gCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTACGCCTCCCACAtt                                                                                                                      >  1:529947/1‑140 (MQ=255)
                          ccGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATtctc                                                                                                                   >  2:4118915/1‑140 (MQ=255)
                             tataCAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCCCATTCTCttt                                                                                                                >  1:2239559/1‑140 (MQ=255)
                             tataCAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCttt                                                                                                                >  1:2357454/1‑140 (MQ=255)
                                  aaTCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTATTTTTCaa                                                                                                           >  2:1187818/1‑140 (MQ=255)
                                   aTCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAg                                                                                                          >  2:4746062/1‑140 (MQ=255)
                                   aTCCCCTCATAATGCTCAGCAAAGTGGGCGGGCGGGTAAAGACTGACCATCAGAATAAACCATTGTAATGATTCCTCCAACAACCCTGACCAGAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAg                                                                                                          <  2:501654/140‑1 (MQ=255)
                                    tCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGc                                                                                                         >  1:3778109/1‑140 (MQ=255)
                                    tCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGc                                                                                                         >  2:3607660/1‑140 (MQ=255)
                                    tCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGc                                                                                                         <  2:4406684/140‑1 (MQ=255)
                                    tCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGc                                                                                                         <  2:1612810/140‑1 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCTTGCGGGTAAACACTGACCATAAGAAAAAACCATTTTAATGATTCCTCCAAAAACACTGAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCAACATTCTCTTTTTCAAGctgt                                                                                                      >  1:2657623/1‑137 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATACACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                                      >  2:3701908/1‑140 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                                      >  1:5176567/1‑140 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                                      >  2:2863114/1‑140 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                                      >  1:3081919/1‑140 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                                      >  2:1341588/1‑140 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                                      >  2:3160881/1‑140 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACg                                                                                                      >  2:2289124/1‑140 (MQ=255)
                                       cGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAAGAAACCATTTTAATGATTCCTCCAAAAACACTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCCAGCACg                                                                                                      >  2:861015/1‑140 (MQ=255)
                                        gTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGt                                                                                                     <  2:3143756/140‑1 (MQ=255)
                                         tCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTa                                                                                                    <  1:4999711/140‑1 (MQ=255)
                                         tCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTa                                                                                                    >  1:1126951/1‑140 (MQ=255)
                                          cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                                                   <  2:4327063/140‑1 (MQ=255)
                                          cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                                                   <  2:4456180/140‑1 (MQ=255)
                                          cATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAc                                                                                                   <  1:1867360/140‑1 (MQ=255)
                                          cATAATGCACATCAAAGTGGGCGTGCGGGTAAACACTGACCATCACAATAAACCATTTTAATGATTCCGCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCGCCCACATTCTCTTTTTCAAGCACGTAc                                                                                                   <  1:4123796/140‑1 (MQ=255)
                                            tAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTg                                                                                                 <  2:35804/140‑1 (MQ=255)
                                                   catcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAAc                                                                                          >  1:959045/1‑140 (MQ=255)
                                                   catcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTATCTTTTTCAAGCACGTACTGGATAAAc                                                                                          >  2:2368846/1‑140 (MQ=255)
                                                    atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCGTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACa                                                                                         >  1:2779117/1‑140 (MQ=255)
                                                    atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGc                                                                                                         >  1:1235681/1‑124 (MQ=255)
                                                    atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACa                                                                                         >  1:3059247/1‑140 (MQ=255)
                                                    atcaAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACa                                                                                         >  1:3716384/1‑140 (MQ=255)
                                                           tGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTggg                                                                                  <  2:191443/140‑1 (MQ=255)
                                                            gggCGTGCGTGTAAAAACTGACAATCAGAATAAACAATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCGTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGa                                                                                 <  1:1409747/140‑1 (MQ=255)
                                                            gggCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGa                                                                                 <  2:274876/140‑1 (MQ=255)
                                                            gggCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGa                                                                                 <  2:4570386/140‑1 (MQ=255)
                                                            gggCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGa                                                                                 <  2:3391472/140‑1 (MQ=255)
                                                              gCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCa                                                                                                            >  1:645553/1‑111 (MQ=255)
                                                              gCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCa                                                                                                            >  1:3218036/1‑111 (MQ=255)
                                                                 tGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATAc                                                                            <  2:2239559/140‑1 (MQ=255)
                                                                   cGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACaa                                                                          >  2:1854464/1‑140 (MQ=255)
                                                                               gACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTa                                                              >  2:2640322/1‑140 (MQ=255)
                                                                               gACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTa                                                              >  2:596351/1‑140 (MQ=255)
                                                                               gACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTa                                                              >  2:1444311/1‑140 (MQ=255)
                                                                                aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGCAATACAAGAACAACGCCTAg                                                             >  1:3509574/1‑140 (MQ=255)
                                                                                aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                                      >  2:170367/1‑131 (MQ=255)
                                                                                aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCACCTCCCACATTCTCTTTTTCAAGCACGTa                                                                                                    <  2:3529217/101‑1 (MQ=255)
                                                                                aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGAAAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCATGCACGTACTGGATAAACATTGTGGGACATACAAGAACACGCCCTAg                                                             >  2:4657483/1‑140 (MQ=255)
                                                                                     cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                        >  2:214891/1‑140 (MQ=255)
                                                                                     cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                        >  2:955459/1‑140 (MQ=255)
                                                                                     cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                        >  1:92910/1‑140 (MQ=255)
                                                                                     cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAAAAACCCCTAGaaaaa                                                        >  2:3212603/1‑140 (MQ=255)
                                                                                       taaTAAACCATTTTAATTATTCCACAAAAAACCATTAAGATAAAAAATAAACTAAAACGCTTCCGCCTCCCACATTCTCTTTTTAAAGAACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAacac                                                      <  2:3081913/139‑1 (MQ=255)
                                                                                        aaTAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACg                                                     >  2:1794216/1‑140 (MQ=255)
                                                                                          tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                   >  1:273378/1‑140 (MQ=255)
                                                                                              ccATTTTAATTATTCATCCAAAAACCCTGAAGATAAAAAAGAACCGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCACGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGaa                                               <  2:2398054/140‑1 (MQ=255)
                                                                                                aatttAATGATTCCTCCAAAAACCATCAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCAACATTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGc                                             <  2:3362100/138‑1 (MQ=255)
                                                                                                aTTTTATTGATTCCTCCAAAAACCCTGAACATAAAAAACAAACTAAAAAGCGTCCGCCTCCAACATTCTCTTTTTAAAGCACGTACTGTATAAACATTGTGGTAAAAACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                             <  2:3325994/140‑1 (MQ=255)
                                                                                                aTTTTAATTATTCCTCCAAAACCCCGAAACAAAAAAAAAAACCCACAAAGCTTCCGCCTCCAACATTCTATTTTTAAAGCACGTACTGGATAAACATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGc                                             <  1:2938906/140‑1 (MQ=255)
                                                                                                aTTTTAATTATTCCACAAAAAACCCTGAAGATAAAAAACAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTTTGGGAAATCCAACAACACCCCCTATAAAAAACGACCGAAGc                                             <  2:205403/140‑1 (MQ=255)
                                                                                                  tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                           >  1:2130621/1‑140 (MQ=255)
                                                                                                  tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                           >  1:4419491/1‑140 (MQ=255)
                                                                                                  tttAATGATTCCTCCAAAAACACTGAAGATAAAAAAGTAACGAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAAAAACCACTAGAAAAAACGACCGACGCaa                                           >  1:2428042/1‑139 (MQ=255)
                                                                                                   ttATTGATTCCCCCACAACCCCTGAATCTAAAAAACAAACCAAAACGCGCCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGTAAATCCAACAACAACCCCTACAAAAAACGACCGAAGCAcc                                          <  1:4298929/140‑1 (MQ=255)
                                                                                                   ttAATTAGTCCTCAAAAAACACTCAATATAAAAAATAAACCAAACAGCTTCCGCCTCCCACATTCTCTTTTTAAAGCAGGTACTGTATAAAAATTGTGGCAAATACAAGAACAACCCCTATAAAACACGACCGAAGCAcc                                          <  1:4746062/140‑1 (MQ=255)
                                                                                                   ttAATGTTTCCTCAAAAAACCCTCAATAAAAAAAACAAACCAAAAAGCTACCGCCACCAACATTCTATTTTTAAAGCACGTACTTTATAAACTTTGTGTCAAATACAACAACACCCCCTAGAAAAAACGACCGAAGCAcc                                          <  1:1701955/140‑1 (MQ=255)
                                                                                                   ttAATGATTCCTCCAAAACCCGTTAACATAAAAAAGAAACCAAAACGCTTCCGCCTCCCACATTATCTTTTACAAGCACGTACTGGATAAACATTGGGGTAAATCCACCAACACCCCCTAGAAAAAACGACCGAAGCAcc                                          <  1:3119290/140‑1 (MQ=255)
                                                                                                   ttAATGAGTCCTCCAAAAACCCTTAAGATAAAAAAGAAACGAAAACGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCAcc                                          <  2:247224/140‑1 (MQ=255)
                                                                                                   ttAAGGATTCCCCCAAAAACCCTGAAGCTAAAAAACAAACGAAAAAGCTGCCCCCGCCCCCATTCTCTTTTTCAAGCACGTACGGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCAcc                                          <  2:793844/140‑1 (MQ=255)
                                                                                                   taaaTGATTCATACAAAAACCATCAAGATAAAAAATAAACGAAAAAGCTACCGCCTCCCAAATTATATTTTTCAAGCACGTACTGTATAAACATTGTGGGAAATACAAGACCAACCCCAATAAAAAACGACCGAAGCAcc                                          <  1:4548452/138‑1 (MQ=255)
                                                                                                       tGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCagat                                      >  2:977121/1‑140 (MQ=255)
                                                                                                           tcctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATCTGTAc                                  >  2:326037/1‑140 (MQ=255)
                                                                                                           tcctcaAAAATCCCTGTATATATAAAATAAAGTATAATGCTTCCGCCTCCGATATTATTTTTTTAAAGCACGTACTGTATTAACATTGTGGGAAATACAAGAACAACCCCTAGATAAAACGACCGAAGCACCATATGTAc                                  <  2:3786214/140‑1 (MQ=255)
                                                                                                            cctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACa                                 >  1:3959691/1‑140 (MQ=255)
                                                                                                            cctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACa                                 >  2:1573445/1‑140 (MQ=255)
                                                                                                                cAAAACCCCTTAAGATAAAAAAGAAACGAAAACGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAATCGACCGAAGCACCATATGTACAGGtt                             <  2:1287847/140‑1 (MQ=255)
                                                                                                                cAAAACCCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                             <  2:2165185/140‑1 (MQ=255)
                                                                                                                cAAAAACCCTTAAGATAAAAAAGAACCTAAAAAGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGCATAAACATTGTGGTAAATACAACAACAACCCATACAAAAAACGACAGAAGCACCATATGTACAGGtt                             <  2:1045389/140‑1 (MQ=255)
                                                                                                                cAAAAACCCTGAACATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAATGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                             <  2:4218454/140‑1 (MQ=255)
                                                                                                                cAAAAACCCTCATGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTAGTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                             <  2:1091788/140‑1 (MQ=255)
                                                                                                                aaaacaCCCTGAAGATAAAAAACAAACGAAAAAGCTTCCGCCTCCAACATTCTCTTTTTCAAGAACGTACTGGATAAACATTTTGGTAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                             <  2:698828/139‑1 (MQ=255)
                                                                                                                aaaaaaCCCTTAACAAAAAAAACAAACCAAAACGCGCCCCCCTCCCACTTTCTCTTTTTCAAGCACGTACTGTATAAACTTTGTGGTAAATACACCAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                             <  1:106266/139‑1 (MQ=255)
                                                                                                                aaaaaaCCCTCAATATAAAAAACAAACCAAAAAGCTCCCGCCACCCAAATTCTCTTTTTCACGCACGTACTGTATAAAAATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                             <  1:3622277/139‑1 (MQ=255)
                                                                                                                aaaaaaCCATTAATATAAAAAACAAACTAACAAGCTGCCGCCTCCCACTTTCTATTTTTCAAGCACGTACTGGATAAACAGTGTGGTAAAGACAAGAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                             <  1:1573445/139‑1 (MQ=255)
                                                                                                                aaaaaaCAATTAAAATAAAAAAGAAACTTAAAAGATTCCGCCTCCCACATTCTCTTTTTCAATCACGTACTGGATAAACTTTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                             <  2:3493924/139‑1 (MQ=255)
                                                                                                                     ccccTTAATATAAAAAAGAAACGAAAAAGCTTCCCCATCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATAAAAGAAAAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                        <  2:2501503/139‑1 (MQ=255)
                                                                                                                     aaccTGAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATACTTTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                        <  2:4055223/138‑1 (MQ=255)
                                                                                                                     aCCCTGAAGATAAAAAAGAAACGAAACAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACTTACTGGATAAACATTGTGGGAAATACAAGACCAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                        <  2:481298/140‑1 (MQ=255)
                                                                                                                                aaaaaaGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                                      >  1:1982096/1‑83 (MQ=255)
                                                                                                                                aaaaaaGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                                      >  1:2539769/1‑83 (MQ=255)
                                                                                                                                      caaaCCAAAAAGCTTCCGCCTCCCACATTCTATTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaa       <  1:2487732/139‑1 (MQ=255)
                                                                                                                                      caaaCAAAAAAGCTCCCGCCGCCCACTTTCTCTTTTAAACGCACGTCCTGGAAAACCATTGTGGTAAATACAACAACAACCCCTACAAACAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaa       <  1:1770141/139‑1 (MQ=255)
                                                                                                                                           gAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGAACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa  <  2:4935407/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTGCAAAACCGAGCGATACACTTGCCCCGTATACAATCCCGTCATAATGCTCATCAAAGTGGGCGTGCGGGTAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAA  >  NZ_CP034943/2269845‑2270123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: