Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,270,057 A→C 10.8% V41G (GTT→GGT)  prsW ← glutamic‑type intramembrane protease PrsW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,270,0570AC10.8% 546.5 / 8.9 251V41G (GTT→GGT) prsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base A (82/129);  new base C (2/38);  total (84/167)
Fisher's exact test for biased strand distribution p-value = 6.48e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

GACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTT  >  NZ_CP034943/2269924‑2270196
                                                                                                                                       |                                                                                                                                           
gACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTa                                                                                                                                       >  2:2640322/1‑140 (MQ=255)
 aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAATGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTGATGCACGTCCTGGATAAACATTGTGGGAAATACAAATACAACACCTAg                                                                                                                                      >  2:3184218/1‑140 (MQ=255)
 aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTCCTGGATAAACATTGTGTGAAATACAAGAACAACCCCtcg                                                                                                                                      >  1:2778700/1‑138 (MQ=255)
 aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGCAATACAAGAACAACGCCTAg                                                                                                                                      >  1:3509574/1‑140 (MQ=255)
 aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                      >  1:946922/1‑140 (MQ=255)
 aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGAAAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCATGCACGTACTGGATAAACATTGTGGGACATACAAGAACACGCCCTAg                                                                                                                                      >  2:4657483/1‑140 (MQ=255)
      cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAATAAAGTACTGGATAAACATTGTGGGAAATACAAGAACAAACCCAAGaaaaa                                                                                                                                 >  2:3207107/1‑140 (MQ=255)
      cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                                                                                                 >  2:214891/1‑140 (MQ=255)
      cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                                                                                                 >  2:1428214/1‑140 (MQ=255)
      cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                                                                                                 >  2:1933526/1‑140 (MQ=255)
      cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                                                                                                 >  1:3684142/1‑140 (MQ=255)
        taaTAAACCATTTTAATTATTCCACAAAAAACCATTAAGATAAAAAATAAACTAAAACGCTTCCGCCTCCCACATTCTCTTTTTAAAGAACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAacac                                                                                                                               <  2:3081913/139‑1 (MQ=255)
        gattAAATCATTTTAATTATTAGTCCAAAAACCGTGAACTTAATAATCAACCTAAAAAGCTTCCGCCTCCCAATTTGTCTTTTTCAACCAGGTACTGTATATACTTTGTGTGAATTACCATAAAACCCCCTAGAAAAAAc                                                                                                                               <  2:2232294/137‑1 (MQ=255)
         aaTAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACg                                                                                                                              >  2:1794216/1‑140 (MQ=255)
           tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTCCTGGATAAACATTGTGGGAAATACAAGAACCACCCCTAGAAAAAACGAc                                                                                                                            >  1:1555632/1‑140 (MQ=255)
           tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                            >  1:1642444/1‑140 (MQ=255)
           tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                            >  1:273378/1‑140 (MQ=255)
           tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                            >  1:588919/1‑140 (MQ=255)
              aCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGa                                                                                                                         >  1:2580411/1‑140 (MQ=255)
               ccATTTTAATTATTCATCCAAAAACCCTGAAGATAAAAAAGAACCGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCACGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGaa                                                                                                                        <  2:2398054/140‑1 (MQ=255)
                aaTTTTAATGATTCCTCAAAAAACCCTTAATATAAAAAATAAACGAAAAAGCTACCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCGAAg                                                                                                                       <  2:4178411/139‑1 (MQ=255)
                 aatttAATGATTCCTCCAAAAACCATCAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCAACATTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGc                                                                                                                      <  2:3362100/138‑1 (MQ=255)
                 aTTTTCATGATTCCTCCAAAAACACTTAATATAAAAAATAAAGGAAAAAGCTTCCGCCTCCAACTTTCTCTTTTTCAAGCACGTACGGGATAAACATTGTGGGAAATACAATAAAAACCCCGACAAAAAACGACCGAAGc                                                                                                                      <  2:2260972/140‑1 (MQ=255)
                 aTTTTATTGTTTCCTCCAAAAACCCTGAAGATAAAAAAGAAACCAAAAAGGTTCCGCCTCCAAAATTCTCTTTTTAAAGCACGTACTTGATATACATTGTGGGAAATTCAAGACCAACCCCTAGAAAAAACGACCGAAGc                                                                                                                      <  2:2473825/140‑1 (MQ=255)
                 aTTTTAATTATTCCACAAAAAACCCTGAAGATAAAAAACAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTTTGGGAAATCCAACAACACCCCCTATAAAAAACGACCGAAGc                                                                                                                      <  2:205403/140‑1 (MQ=255)
                 aTTTTAATGATTCCTCAAAAAACCCTTAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGGATAAACATTTTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                      <  2:791081/140‑1 (MQ=255)
                 aTTTTAATGATTCCTCAAAAAACCCTCAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCAACATTCTCTTTTTCAAGAACTTACTGGATAAACCTTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                      <  2:3671135/140‑1 (MQ=255)
                 aTTTTAATGATTCATCAAAAAACCCTGAATATAAAAAAGAAACTAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCGAAGc                                                                                                                      <  2:478464/140‑1 (MQ=255)
                   tttAATGATTCCTCCAAAAACCCTGAATAAAAAAAAGAAACGAAAACGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAAGACCAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                    <  2:961206/140‑1 (MQ=255)
                   tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAATAACAACCCCTAGAAAAAACGACCGAAgacc                                                                                                                    >  1:4789397/1‑137 (MQ=255)
                   tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                    >  2:3287439/1‑140 (MQ=255)
                   tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                    >  2:4308532/1‑140 (MQ=255)
                   tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                    >  1:3866900/1‑140 (MQ=255)
                   tttAATGATTCCTCCAAAAACACTGAAGATAAAAAAGTAACGAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAAAAACCACTAGAAAAAACGACCGACGCaa                                                                                                                    >  1:2428042/1‑139 (MQ=255)
                    ttATTGATTCCCCCACAACCCCTGAATCTAAAAAACAAACCAAAACGCGCCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGTAAATCCAACAACAACCCCTACAAAAAACGACCGAAGCAcc                                                                                                                   <  1:4298929/140‑1 (MQ=255)
                    ttAATGATTCCTCCAAAACCCGTTAACATAAAAAAGAAACCAAAACGCTTCCGCCTCCCACATTATCTTTTACAAGCACGTACTGGATAAACATTGGGGTAAATCCACCAACACCCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                   <  1:3119290/140‑1 (MQ=255)
                    ttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                   <  2:2357454/140‑1 (MQ=255)
                    ttAATGATTCATCCAAAAACCGTGAAGATAAAAAAGAAACCAAAAAGCTTCCGCCACCCACATTCTCTTTTTCAAGCACTTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                   <  2:3591789/140‑1 (MQ=255)
                      aagtaTTCCACCAAAACCCAGGAAGACAAAAAACAAACCAAAACCCTCCCGCCTCCCACATTCTCTTTTACAAGCACGTACTGTATAAAATTGGGGGTAAATACAAGAACACCCCCTATAAAAAACGACCGAAGCACCat                                                                                                                 <  1:4071683/136‑1 (MQ=255)
                      aaTGATTCCTCCAACAACCCTTAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCAAAATTCTCTTTTTCACGCACGTAGTGGATAACCTTTGTGGTAAATCCAAGAACACCCCCTAGAAAACACGACCGAAGCACCat                                                                                                                 <  2:345160/140‑1 (MQ=255)
                         gATTCCTCCAAAAACCCTGAAGATAAAAGAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATg                                                                                                              >  2:4395093/1‑140 (MQ=255)
                          aTTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGt                                                                                                             >  2:3755221/1‑140 (MQ=255)
                            tcctcaAAAATCCCTGTATATATAAAATAAAGTATAATGCTTCCGCCTCCGATATTATTTTTTTAAAGCACGTACTGTATTAACATTGTGGGAAATACAAGAACAACCCCTAGATAAAACGACCGAAGCACCATATGTAc                                                                                                           <  2:3786214/140‑1 (MQ=255)
                                 cAAACACCCGTAACATAAAAAACAAACGAAAAAGCGTCCGCCGCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGTAAATACAACAAAAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  2:4043630/140‑1 (MQ=255)
                                 cAAAACCCCTTAAGATAAAAAAGAAACGAAAACGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAATCGACCGAAGCACCATATGTACAGGtt                                                                                                      <  2:1287847/140‑1 (MQ=255)
                                 cAAAAACCCTTAATATAAAAAACAAACTAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  2:828979/140‑1 (MQ=255)
                                 cAAAAACCCTTAAGATAAAAAAGAACCTAAAAAGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGCATAAACATTGTGGTAAATACAACAACAACCCATACAAAAAACGACAGAAGCACCATATGTACAGGtt                                                                                                      <  2:1045389/140‑1 (MQ=255)
                                 cAAAAACCCTGAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGTATAAACATTGTGGGAAATACACGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  2:1149242/140‑1 (MQ=255)
                                 cAAAAACCCTGAACATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTAATGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  2:4218454/140‑1 (MQ=255)
                                 aaacaaCCCGAAAAATAAAAAAAAAACCAAAAAGCTCCCGCCTCCAAAATTCTATTTTTCAAGCCCGTACGGTAAAACACTTGTGGGAAATACAACACCACCCCCTCAAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  1:1756810/136‑1 (MQ=255)
                                 aaaacaCCCTGAAGATAAAAAACAAACGAAAAAGCTTCCGCCTCCAACATTCTCTTTTTCAAGAACGTACTGGATAAACATTTTGGTAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  2:698828/139‑1 (MQ=255)
                                 aaaaaaCCCTTAATATAAAATAAAAACTAAAAAGCTGCCGCCACCCAAATTCTATTTTTAAAGCACTTAGTGTATAAACTTTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  1:4395093/139‑1 (MQ=255)
                                 aaaaaaCCCTTAATATAAAAAAAAAAACAAAAAGCTCCCCCCTCCCAATTTATATTTTACAAGCACTTACTGTATAAAATTTTTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  1:2157354/139‑1 (MQ=255)
                                 aaaaaaCCCTTAACAAAAAAAACAAACCAAAACGCGCCCCCCTCCCACTTTCTCTTTTTCAAGCACGTACTGTATAAACTTTGTGGTAAATACACCAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  1:106266/139‑1 (MQ=255)
                                 aaaaaaCCCTCAATATAAAAAACAAACCAAAAAGCTCCCGCCACCCAAATTCTCTTTTTCACGCACGTACTGTATAAAAATTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                      <  1:3622277/139‑1 (MQ=255)
                                   aaaaCCCTTAATATAAAAAACAAACTAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAACAATTGTGGCAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCa                                                                                                    <  2:1585963/140‑1 (MQ=255)
                                      ccccTTAATATAAAAAAGAAACGAAAAAGCTTCCCCATCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATAAAAGAAAAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                                 <  2:2501503/139‑1 (MQ=255)
                                      aCCCTTAATATAAAAAATAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGGAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                                 <  1:4447052/140‑1 (MQ=255)
                                      aCCCTGAATATAAAAAATAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                                 <  2:1551976/140‑1 (MQ=255)
                                                       caaaCCAAAAAGCTTCCGCCTCCCACATTCTATTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaa                                                                                <  1:2487732/139‑1 (MQ=255)
                                                         aaCGAAAACGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACTTACGGTATAAAATTTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaat                                                                              <  2:311060/140‑1 (MQ=255)
                                                            gAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGTTTAAACATTGTGGTAAATAAAACAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                           <  2:1076558/140‑1 (MQ=255)
                                                            caaaaaGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGTATAAACTTTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                           <  2:3340371/139‑1 (MQ=255)
                                                              aaacGCTTCCGCCTCACACATTATTTTTTTAAACCACTTAGTGTAAAACCTTTTTGTTAAATAAAATAACAACCCCTATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTaaataaaaa                                                                         <  1:291735/140‑1 (MQ=255)
                                                                      ccGCCTCCCAATTTCTCTTTTTAAAGCACGTACTGGATAAACTTTGTGGTAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTaa                                                                 <  1:2895108/140‑1 (MQ=255)
                                                                       ctccTCCCATATTATCTTTTTCAAGCACGTACTTGTTAAAAATTGTGGTAAATACAATAACAACCCGTATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAAc                                                                <  2:92910/138‑1 (MQ=255)
                                                                            cccACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAgcgc                                                           >  1:1790934/1‑140 (MQ=255)
                                                                            cccACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAgcgc                                                           <  2:946922/140‑1 (MQ=255)
                                                                                  ttCTCTTTTTAAAGCACGAACTGGATAAACTTTGTGGGATATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGcc                                                     <  2:4961241/140‑1 (MQ=255)
                                                                                  ttCTCTTTTACAAGCACGTCCGGTATAACCATTGTGGGAAATACACGAACACCCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGcc                                                     <  1:874355/140‑1 (MQ=255)
                                                                                     tctTTTTCAAGCACTTACTGGATAAAAATTGTGGCAAATACAACAAAAACCCATACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCggg                                                  <  1:1854464/140‑1 (MQ=255)
                                                                                        ttttCAAGCACGTACTGTATAAACATTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                               <  2:1743233/140‑1 (MQ=255)
                                                                                        ttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                               <  2:1627288/140‑1 (MQ=255)
                                                                                        ttttCAAGCACGTAATGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                               <  2:4063529/140‑1 (MQ=255)
                                                                                           taaaGCACTTACTGCATAAACATTGTGGTAAATACAACAACAACCCCAACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                            <  1:1966555/138‑1 (MQ=255)
                                                                                           tCAAGCACGTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                            <  1:3278618/140‑1 (MQ=255)
                                                                                           tCAACCACGTACTGTATAAACATTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                            <  1:2242255/140‑1 (MQ=255)
                                                                                                cACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTg                                       <  2:545133/140‑1 (MQ=255)
                                                                                                cACGTACTGGATAAACATTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTg                                       <  2:273378/140‑1 (MQ=255)
                                                                                                  cGTCCTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACAATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCa                                     <  2:2796941/140‑1 (MQ=255)
                                                                                                  cGTACTGGATAAACTTTGTGGGAAATACAAGAACAACCCCTATAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCa                                     <  2:4419491/140‑1 (MQ=255)
                                                                                                  cGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCa                                     <  2:3866900/140‑1 (MQ=255)
                                                                                                         gATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCAAACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgatga                              >  2:3493549/1‑140 (MQ=255)
                                                                                                           tAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAtt                            >  2:4609375/1‑140 (MQ=255)
                                                                                                              aCATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                         <  1:1219708/140‑1 (MQ=255)
                                                                                                              aCATTGTGGGAAATAAAACCACAACCCCTAGAAAAAACGACCGCAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACCGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                         <  1:813786/140‑1 (MQ=255)
                                                                                                                  ttGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAAc                      >  1:983808/1‑140 (MQ=255)
                                                                                                                   tgtgGCAAATACAAGAACAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACa                     <  1:722387/140‑1 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGATCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                    >  1:3006662/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                           >  1:1487812/1‑85 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                           <  2:1487812/85‑1 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                    >  1:4719805/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                    >  2:5069027/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                    >  2:2705435/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                    >  1:1903786/1‑140 (MQ=255)
                                                                                                                    gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                    >  1:4585672/1‑140 (MQ=255)
                                                                                                                          aaaTACAAGAACAACCCCAAGAAAACACGACCGAAGCACCAAATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATACCTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAACAGGc               <  2:4892707/140‑1 (MQ=255)
                                                                                                                                 aGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct        <  2:3695232/140‑1 (MQ=255)
                                                                                                                                      aacCCCTATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGACGATTGCAAACATCAGGCAACCTCTTTCCt   <  2:202473/140‑1 (MQ=255)
                                                                                                                                       acCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCtt  >  1:502284/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
GACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTT  >  NZ_CP034943/2269924‑2270196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: