Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,387,066 T→G 12.6% S354R (AGC→CGC)  EO946_RS12195 ← acetyl‑CoA C‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,387,0660TG12.6% 554.2 / 6.1 259S354R (AGC→CGC) EO946_RS12195acetyl‑CoA C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (103/104);  new base G (46/2);  total (153/106)
Fisher's exact test for biased strand distribution p-value = 2.35e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAC  >  NZ_CP034943/2386927‑2387204
                                                                                                                                           |                                                                                                                                            
ccccTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCt                                                                                                                                              <  1:148855/140‑1 (MQ=255)
        tCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCAAGACTATTATCGCACCGCTTGCGCCGa                                                                                                                                      >  2:4050267/1‑140 (MQ=255)
           tCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAAATAGTAAAAAAGAGTAATTATTATTATCGCACCCCTTGCGcccctag                                                                                                                                   >  1:131473/1‑135 (MQ=255)
                        aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGc                                                                                                                      <  2:2569938/140‑1 (MQ=255)
                        aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGc                                                                                                                      <  1:3355735/140‑1 (MQ=255)
                        aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAACTGTGATGAGTTTTAGCGCACCCCTTGCGCCGATCGGGTGGCCGAGGGc                                                                                                                      >  1:5035225/1‑140 (MQ=255)
                        aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTACTAAAAAACTTTGATGAGGATTCTCGCCACCCGTGCCCCCAGCGGGGGGGCGCGGGc                                                                                                                      >  1:700441/1‑140 (MQ=255)
                        aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCCTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGCTCGGGTGGCCGAGGGc                                                                                                                      <  1:432251/140‑1 (MQ=255)
                         cTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCCTAAACAAATGTCATGAGTATTCTAGCACCCCGTGCGCCGATCGGGTGGCCGAGGGCg                                                                                                                     >  2:3657650/1‑140 (MQ=255)
                          ttGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGa                                                                                                                    <  2:4487470/140‑1 (MQ=255)
                           tGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGAt                                                                                                                   <  2:3688521/140‑1 (MQ=255)
                                tAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcac                                                                                                             <  1:866953/140‑1 (MQ=255)
                                tAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcac                                                                                                             <  2:1241745/140‑1 (MQ=255)
                                gaaGACGGCGTCACCCTGAGCGGCACCGCTGCAAATGGCGGCACCACCCAGTCCCCCGCCCCGTCGCTTTAAGTCGTACACAAGTGTCATGAGTATTCTCGCCCCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcac                                                                                                             <  2:4598611/139‑1 (MQ=255)
                                 aaGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcacc                                                                                                            <  2:2808363/140‑1 (MQ=255)
                                 aaGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCACTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcacc                                                                                                            <  2:2983996/140‑1 (MQ=255)
                                  aGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCGTCCCCGTCGCTTTAATTCGTAAAAAAGTGTGATGCGGTGTCTCGGACCGCGGGCGCCGCTCGGGTGGGCGGGGGCGATGGctccg                                                                                                           >  2:3079048/1‑139 (MQ=255)
                                      ggCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGt                                                                                                      <  1:1286360/140‑1 (MQ=255)
                                      ggCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGt                                                                                                      <  1:1441747/140‑1 (MQ=255)
                                       gCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGtt                                                                                                     <  2:2699816/140‑1 (MQ=255)
                                       gCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAACAACTGTCATGAGTTTTCTCGCACCCCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCcccccccgg                                                                                                     >  1:4234017/1‑137 (MQ=255)
                                        cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACCAGTGTCATGAGTATTCTGGCCCCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCCCCACCGttt                                                                                                    >  2:3402037/1‑140 (MQ=255)
                                        cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTGTCGCCCCCCGTGCGCCCAACGGGTGGCCGAGGGCGATCGCACCACCGgtt                                                                                                    >  2:1732723/1‑140 (MQ=255)
                                        cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCCCCGCTTGGGCCGCTCGGGGGGCCGCGGGCGATGGCACCACCGgtt                                                                                                    >  2:4111471/1‑140 (MQ=255)
                                        cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTAATCGTCAACAACTGTGAAGAGTAATCTCCCACCCCGTGCGCCGAAAGGGGGGGCGAGGGCGATCGCAACAACCGtt                                                                                                     >  1:1509800/1‑140 (MQ=255)
                                        cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTGAGTCGTAAACAAGTGTCATTAGTATTCTCGCACCGCTTGCGCCGAAAGGGTGGCCGAGGGCGAGCGCACCCCCGgtt                                                                                                    >  2:1551090/1‑140 (MQ=255)
                                          tCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAACAAGTGTCATGAGTAATATCGCACCCCATTCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTaa                                                                                                  >  2:2333644/1‑139 (MQ=255)
                                                  aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTAGTAAACAAGTGTGATGAGTATTCTGGCACCGCGTGGGCCGATCGGGTGGGCGCGGGGGGAAGCCCCACCCGTTACCTTTAcat                                                                                          >  1:4840011/1‑138 (MQ=255)
                                                  aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAAAAAGTGTCATGAGTATTATCGCACCCCGTGCGCCCATCGGGTGGCCCAGGGCGATCGCCCCACCGGTGACATTTAAtt                                                                                          >  1:1688154/1‑140 (MQ=255)
                                                  aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAAATCGTAAAAAAATGTGAAGAGTAGTGTCGCACCCCGTGCGCCGCACCGGGGGGCGCGGGCGCACGCCCCACCCGTTTACAttaatt                                                                                           >  1:4446336/1‑136 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTTATTCTTAAACAAGTGTCAGGATTATTATCGCACCGCTGGGGCCCATAGGGTGGGCGAGGGCGGTCGCACCACCCGTTACAGTTACtttttta                                                                                     >  1:5126581/1‑138 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTTATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCGTGCGCCGCACGGGGGGGCGCGGGCGATCCCACCACCGTTTACAGTTACTTTTTCa                                                                                     >  2:819830/1‑140 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTTATTCGTAAAAAAGTGTCATGATTATTATCGCCACCCGGGCGCCGATCGGGTGGCCCCGGGCGATCGCACCACCGGTTAAATTTACtttttaa                                                                                     >  1:1042777/1‑138 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTATTTCCTAAACAAGTGTCATGTGTATTCTAGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCCCCACCGGTTACATTTGATTTTTCa                                                                                     >  2:3905966/1‑140 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTGGTAAACAAGTGTGATTAGTTTTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTTCATTTACTTTTTCa                                                                                     >  2:27124/1‑140 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGCTCGCACCACCCGTTACAGTTACTTTTTCa                                                                                     >  2:2759131/1‑140 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAAAAAGTGTGATGCGTATTCTCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCCTTTACATTTACTTTTTCa                                                                                     >  2:3203848/1‑140 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTCGTAAAAAAGTGTCATGAGGATTCTCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTACAATTACtttttaa                                                                                     >  1:1117336/1‑138 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAAAAAGTGTGATGTGTATTATCGCACCCCGTGCGCCGATCGGGTGGCCGAGGGCGGTCGCCCCACCCGGTGCATTTTATTTTTCa                                                                                     >  1:2486027/1‑140 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCCTAAACAAGTGGCCTGAGGCTTCTCGCACCGCGTGCGCCGAGCGGGGGGCCGCGGGCGGTCGCAGCCCCCGGTAGATTTACtttttga                                                                                     >  1:5183443/1‑138 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGGTAATTGGTAAACAACTGTCGTGAGTATTGTCGCACCGCGTGCGCCGCTCGGGGGGGCGGGGGCGATCGCCCCACCGGGTGCATTTTGtttttga                                                                                     >  1:316458/1‑138 (MQ=255)
                                                       cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGGCGCGTTAATTCGTAAAACACTGTCATGAGTATTATCGCCCCGCGTGCGCCGCTCGGGGGGGCGCGGGCGCTGGCCCCCCCGGTGTCATTTTAtttttga                                                                                     >  1:571499/1‑138 (MQ=255)
                                                            cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAAAAAGTGTCAATAGTATTCTCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTAAATTTACATTTTCAATATc                                                                                >  2:4991985/1‑140 (MQ=255)
                                                            cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTAGTAAAAAAATGTCATGATTATTATCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCCGTTTCAATTAATTTTTAAAAATc                                                                                >  1:1212522/1‑140 (MQ=255)
                                                                               ccAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                                                             <  2:3049325/140‑1 (MQ=255)
                                                                                cAGTCCCCCTCCCCGTCGCTTTAATTAGTAAAAAAGTGTCATGAGTATTCACGCACCGCTTGCGCCGATCGGGGGGCCGAGGGCGATAGCACCACCCGTTACCTTTTCTTTTTCAAGAAAGAAAACCAAGATTTTTTTAc                                                            >  2:5225348/1‑140 (MQ=255)
                                                                                   tCCCCCTCCCCGTCGCTTTAATTCCTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATAGGGTGGCCGAGGGCGATCGCACCACCGTTTTCCATTAATTTTTAAAGATAGAAAACGACGAATTTTTTAAATg                                                         >  2:3265461/1‑140 (MQ=255)
                                                                                    cccccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGt                                                        <  2:4885542/140‑1 (MQ=255)
                                                                                       ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa                                                     <  1:1962576/140‑1 (MQ=255)
                                                                                            ccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa                                                <  1:2707955/140‑1 (MQ=255)
                                                                                             cGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcacca                                                                                                           >  2:3818333/1‑81 (MQ=255)
                                                                                             cGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcacca                                                                                                           <  1:3818333/81‑1 (MQ=255)
                                                                                              gTCCCTTTAATTCGTCAACAAGTCTCATGAGTAGTCTCGCACCGCTTGCGCCGCTCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTGTTCAAGATCGAACCCGACGATTTTTTTACATGTCAAaacaacc                                              <  2:4865228/140‑2 (MQ=255)
                                                                                               tCGCGTTAATTCGTAAACAACTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGGTCGCACCACCGGTTACCTTTAATTTTTCAAGATCGAAACCGACGATTTTTTTAAATGTCAAaacctgtg                                             >  2:2359345/1‑135 (MQ=255)
                                                                                                   tttAATTCGTAAACAAGTGTCATGATTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATAGCACCACCCGTTACATTTAATTTTTAAAGATAGAAAACGACGATTTTTTTAAATTTCAAAACAAAAGAAGc                                         >  2:1896475/1‑140 (MQ=255)
                                                                                                            tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                >  2:216405/1‑140 (MQ=255)
                                                                                                            tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                >  1:4977086/1‑140 (MQ=255)
                                                                                                            tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTAACATGTCAAAACAACAGAAGCAAACGCTTc                                >  1:5159400/1‑140 (MQ=255)
                                                                                                              aaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTAAATTTACTTTTTCAAGATCGAAACAGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAt                              <  1:2619060/140‑1 (MQ=255)
                                                                                                               aCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCCAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAtt                             <  2:2117701/140‑1 (MQ=255)
                                                                                                                      tCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCa                      >  1:3221634/1‑140 (MQ=255)
                                                                                                                      tCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCa                      >  2:3590188/1‑140 (MQ=255)
                                                                                                                        aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                    <  1:3894220/140‑1 (MQ=255)
                                                                                                                        aTGAGAATTCTCGCACCGCTCGCGCCGATCGGGTGGCCGAGCGCGATCGCACCGCCATTCACATTTACTTTTTCAAGATCGAAACCTACGATTTTTTCACATGTCAAAACAACTGAAGCAAAAGCTTCATTTACTTCaaa                    <  1:1013271/140‑1 (MQ=255)
                                                                                                                         tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                   >  2:4809433/1‑140 (MQ=255)
                                                                                                                         tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTGACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCCAATCAACAGAAGCAAACGCTTCATTTACTTCAAAc                   >  2:3444018/1‑140 (MQ=255)
                                                                                                                           aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                 >  1:291466/1‑140 (MQ=255)
                                                                                                                           aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                 >  2:2506655/1‑140 (MQ=255)
                                                                                                                              aTTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                                                             <  1:2186367/93‑1 (MQ=255)
                                                                                                                              aTTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACTCTTCATTTACTTCAAACAAGTc              >  1:3981346/1‑140 (MQ=255)
                                                                                                                              aTTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTc              >  2:2023162/1‑140 (MQ=255)
                                                                                                                              aTTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTc              >  2:5153063/1‑140 (MQ=255)
                                                                                                                               ttCTCGCACCGCTTGCGCCGATCGGGTGTCCGAGGGCGATCGCAACACCGTTTACATTTACTTGTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  2:4684804/140‑1 (MQ=255)
                                                                                                                               ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  1:4045534/140‑1 (MQ=255)
                                                                                                                               ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  2:5147414/140‑1 (MQ=255)
                                                                                                                               ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  2:2643359/140‑1 (MQ=255)
                                                                                                                               gtctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTGACTTTTTCAAGATCGAAACCGACGATTTTTTTACAGGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  2:4355144/139‑1 (MQ=255)
                                                                                                                                tctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCaa            >  1:3058756/1‑140 (MQ=255)
                                                                                                                                tctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCaa            >  1:1832712/1‑140 (MQ=255)
                                                                                                                                  tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             <  1:193019/137‑1 (MQ=255)
                                                                                                                                  tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa             >  2:193019/1‑137 (MQ=255)
                                                                                                                                    gCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATc        >  1:160995/1‑140 (MQ=255)
                                                                                                                                     cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTAGTTCAAACAAGTCAATATCt       >  2:1294568/1‑140 (MQ=255)
                                                                                                                                     cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCt       >  1:467631/1‑140 (MQ=255)
                                                                                                                                     cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCt       >  2:1984660/1‑140 (MQ=255)
                                                                                                                                     cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCt       >  2:879189/1‑140 (MQ=255)
                                                                                                                                       ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAACCCGACGATTTTTTTACAAGTCCAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  2:2534120/140‑1 (MQ=255)
                                                                                                                                       ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  1:1896475/140‑1 (MQ=255)
                                                                                                                                       ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  2:385757/140‑1 (MQ=255)
                                                                                                                                       ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     >  2:3955829/1‑140 (MQ=255)
                                                                                                                                       ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  2:3803758/140‑1 (MQ=255)
                                                                                                                                       ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  1:4764239/140‑1 (MQ=255)
                                                                                                                                       ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg     <  2:1145723/140‑1 (MQ=255)
                                                                                                                                         gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa   <  1:2252468/140‑1 (MQ=255)
                                                                                                                                         gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa   <  2:4574192/140‑1 (MQ=255)
                                                                                                                                          cTTGCGCCGATCGGGTGGCCGAGGGCGCTCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGTCGATTTTTGTACATGTCAAAACAACAGAAGCAAACGCTACATTTAGGTCAAACAAGTCAATATCTTGGAc  >  2:705355/1‑140 (MQ=255)
                                                                                                                                          cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAATTAAATATCTTgtaa  >  2:2189639/1‑137 (MQ=255)
                                                                                                                                          cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc  <  1:2506655/140‑1 (MQ=255)
                                                                                                                                          cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAAAAGTCAATATCTTGGAc  >  1:2584375/1‑140 (MQ=255)
                                                                                                                                          cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGAGCGAAACCGACGATTTTTTTACATGTCACACCAACAGAAGCAAACGCTTCCGTTACTTCAAACACGTCAATATCTTGGAc  >  2:904987/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAC  >  NZ_CP034943/2386927‑2387204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: