Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,242 T→G 21.1% T274P (ACG→CCG)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2420TG21.1% 266.3 / 31.6 175T274P (ACG→CCG) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base T (72/61);  new base G (39/3);  total (111/64)
Fisher's exact test for biased strand distribution p-value = 2.10e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

AATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGA  >  NZ_CP034943/2502107‑2502381
                                                                                                                                       |                                                                                                                                           
aatTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCgtgtgt                                                                                                                                         <  2:3724661/140‑1 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATCGAAATCCCCGtgggtg                                                                                                                                        >  2:3215626/1‑140 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATAGAAATCCCCGtgtgtg                                                                                                                                        >  1:4898960/1‑140 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGtgtgtg                                                                                                                                        >  2:1630380/1‑140 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGtgtgtg                                                                                                                                        >  2:1769148/1‑140 (MQ=255)
 atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGAGTTTAATCGAAATACCCGggtgtg                                                                                                                                        >  2:2693515/1‑140 (MQ=255)
  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAAACGAAATCCCCGTGTGTgg                                                                                                                                       >  2:4905767/1‑140 (MQ=255)
  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATACCCCTGTGTgg                                                                                                                                       >  1:2838978/1‑140 (MQ=255)
  ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTGTCTTTCCCGATGTTAATCGAAATACCCGTGTGTgg                                                                                                                                       >  1:1035199/1‑140 (MQ=255)
   tCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTggg                                                                                                                                      <  2:3323729/140‑1 (MQ=255)
      tGCGGGTCGGACCGATTATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTACATCTCTTCATAGTCGTTTTATTTACCGATTTTAATCTAAATACCCCTGTGTGGGGAt                                                                                                                                   >  2:3874452/1‑140 (MQ=255)
      tGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAt                                                                                                                                   >  2:5040070/1‑140 (MQ=255)
      tGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCCTGTGTGGGGAt                                                                                                                                   >  2:68793/1‑140 (MQ=255)
       gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGCATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGAtt                                                                                                                                  >  1:649574/1‑140 (MQ=255)
           gTCGGACCGATGCTCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAc                                                                                                                              <  1:5117437/140‑1 (MQ=255)
           gTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATGCCCGTGTGTGGGTATTGTAc                                                                                                                              >  2:4553246/1‑140 (MQ=255)
           gTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAc                                                                                                                              <  2:4050248/140‑1 (MQ=255)
           gTCGGACAGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAc                                                                                                                              <  1:4069177/140‑1 (MQ=255)
            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                             <  1:2644159/140‑1 (MQ=255)
            tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                             <  1:2461236/140‑1 (MQ=255)
                aCCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCt                                                                                                                         >  1:3344032/1‑140 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGGGTGTGGGGATTGAACGCGCTTTaaa                                                                                                                    >  1:102362/1‑140 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTTAACGAGCTTTaaa                                                                                                                    >  2:2030512/1‑140 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCCTGTGTGGGGATTGAACGCGCTTTaaa                                                                                                                    >  2:4829132/1‑140 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTATTTGCCGATTTTAATCGAAATACCCGTGTGTGGGGGTTGAAGGAGCTTTaaa                                                                                                                    >  1:1240152/1‑140 (MQ=255)
                     tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAAACCCCGTGTGTGGGGATTGAACGAGCTTTaaa                                                                                                                    >  1:4665227/1‑140 (MQ=255)
                         cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCgtgtgt                                                                                                                                         <  1:2763424/115‑1 (MQ=255)
                         cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAATGAACGAGCTTTAAAACAt                                                                                                                >  2:2733107/1‑140 (MQ=255)
                                   ccGATCTGCTTCTGATCAACGTAATCAGCAGCAGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                      <  1:3859918/140‑1 (MQ=255)
                                   ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATCGAAATACCCGTGTGTGGGGATTTTACGAGGTTTTAAAAATACTGTTGttt                                                                                                      >  1:4267508/1‑140 (MQ=255)
                                   ccGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttt                                                                                                      <  2:3344032/140‑1 (MQ=255)
                                     gATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCttttt                                                                                                    <  1:1132757/140‑1 (MQ=255)
                                      aTCTGCTTCTGATCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTGCCGATTTTAATAGAAATCCCCGTGTGTGGGGCTTGTAGGAGCTTTTAAAAATCCTGTTCTTTTTc                                                                                                   >  1:4872237/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTGATCGAAATACCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCGGTTCttttta                                                                                                   >  1:3294690/1‑139 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGGGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTAttttta                                                                                                   >  1:2013653/1‑139 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATAGAAATACCCGTGGGTGGGGATTGAACGAGCGTTAAAACATCCTGTTCTTTTTc                                                                                                   >  2:2082535/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCCATTTTAATAGAAATCACCGTGTGTGGGGATTGTACGAGCTTTAAAACATCCTTTTATTTTTc                                                                                                   >  1:579818/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCCATTTTAAACGAAATACCCGTGGGGGGGGATTGTCCGAGCTTTAAAACATCCGGTTGtttttg                                                                                                   >  1:4789763/1‑139 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCACGATTTTAATCGAAAAACCCCGGGGTGGGGATTGTACCAGCGTTAAAAAAAACTGTTCTTTTTc                                                                                                   >  1:782069/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTTAAACATCCTGTTCTTTTTc                                                                                                   >  2:3639490/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCGTGTGGGGGGTTTTAAGGTGGTTTTAAACATCCTGTTCTTTTTc                                                                                                   >  1:298729/1‑140 (MQ=255)
                                      aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTATTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCGTTAAAAAATCCTGTTAtttttg                                                                                                   >  1:1373526/1‑139 (MQ=255)
                                        cTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaa                                                                                                 <  1:4933179/140‑1 (MQ=255)
                                          gCTTCTGATCATCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                                               <  2:3013426/140‑1 (MQ=255)
                                          gCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTTCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                                               <  1:4340547/140‑1 (MQ=255)
                                          gCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                                               <  2:2129017/140‑1 (MQ=255)
                                          gCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                                               <  2:1382228/140‑1 (MQ=255)
                                                 aTCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATACCCGGGTGTGGGGATTGACCGAGCGTTAAAAAATCCGGTTCTTTTTACCATAATgaga                                                                                        >  1:4447101/1‑140 (MQ=255)
                                                 aTCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCGTTGCCGATTTTAATCGAAATCCCCGGGGGTGGGGATTGAACGCGCTTTAAAAACGCCTGTTATTTTTCAATTAATgagt                                                                                        >  1:1102063/1‑139 (MQ=255)
                                                 aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAATCGAAATACCCGTGTGTGGGGATTGAACGATCTTTAAAACATCCTGTTCTTTTTCAATTAAttgga                                                                                        >  1:1859995/1‑136 (MQ=255)
                                                 aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAAAATCCGGTTCTTTTTCAATTAATgaga                                                                                        >  2:602493/1‑140 (MQ=255)
                                                 aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTACCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGTCAGTGCGTTAAAAACTGCTGTTCTTTTTCAATTAATgaga                                                                                        >  1:2937431/1‑140 (MQ=255)
                                                     aCGGAATACGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTATTTTTCAATTAATGAGAGAAg                                                                                    <  2:1978554/140‑1 (MQ=255)
                                                     aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATCGAAATCCCCGTGTGTGGGGCTTGAACGAGCTTTAAAACATCCTGGTCTTTTTCAATTAATTAGAGAAg                                                                                    >  2:4897683/1‑140 (MQ=255)
                                                     aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGGGGGGGTTGACCGAGCTTTAAAACATCCGGTTATTTTTCAATTAATgagagacg                                                                                    >  2:4225522/1‑138 (MQ=255)
                                                      cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGGGTGTGGGGATTGAAAGAGCGTTAAAACATCCTGTTATTTTTCAATTAATGAGAGaaac                                                                                   >  2:4020023/1‑138 (MQ=255)
                                                        gAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCGTTCCCCATTTTAATCGAAATCCCCGTGTGTGGGGATTGACCGAGCTTTAAAACCACGGGTTGTTTTTAAATTAATAAGAGAAggca                                                                                 >  2:1940607/1‑137 (MQ=255)
                                                        gAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATCCCCGGGGGGGGGGAGTGAACGAGCTTTAAAACATCCTGTTCGTTTTCAATTAATGTGAGACGCGa                                                                                 >  2:3977785/1‑140 (MQ=255)
                                                               aagCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAct                                                                          <  1:3625266/140‑1 (MQ=255)
                                                               aagCCGTAATCAGACTGCAGTTTTCAATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGATTTAAAACATCCTGTTCTTTTTCAATTAATGAGATAAGCGACCGAAca                                                                          <  2:2362412/140‑2 (MQ=255)
                                                                agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGCTTTTAATCGAAATACCCGTGTGTGGGGATTGTACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGTGAGAAGCGACCGAAcgc                                                                         >  1:3202699/1‑138 (MQ=255)
                                                                agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCCTGTGTGGGGATTTTAAGAGCTTTAAAACATCGTGTTATTTTTGAATTAATGAGAGAAGCGAGCGAActa                                                                         >  1:3065295/1‑139 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAAAGAGAGAATCGACCGAAATCTgg                                                                      >  2:3058711/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGTACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATTAGAGAAGCGACCGAACTCTgg                                                                      >  2:2831133/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                      >  2:1073544/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                      >  2:3134091/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACAGCCTGTGCTTTTTCAATTAATGAGAGTAGCGACCGAAAGCTgg                                                                      >  2:5220017/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGTTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                      >  2:1132528/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGGGGGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTGAATTAATGAGAGAAGCGACCGAACTCTgg                                                                      >  1:1052490/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAAACCCGTGTTTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTAAATTAATGAGAGAAGCGACCGAACTATgg                                                                      >  2:812065/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGGGGGTGGGGCTTGTACGAGCGTTAAAAAATCCTGTTGTTTTTGAATTAATGAGAGAAGCGACCGAACGCTgg                                                                      >  1:3353840/1‑140 (MQ=255)
                                                                   cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGAGTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCATTTACTGAGAGAAGCGACCGCACTCggg                                                                      >  2:4389527/1‑140 (MQ=255)
                                                                   agTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCTGGTTCGTTCCCCATTTTAATCGAACACCCCGTGTGTGGGGCTTTAAAGAGGACTAAAAACTACTGTTAGTTTTCAATTAACGATAGAAGCCACCGCAATCTgg                                                                      >  1:4353510/2‑140 (MQ=255)
                                                                     tAATCAGACTGCAGTTTTCCATCTGTTCATAGTCGTTTTAATTACCGAGTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGCGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAAAAACGACCGAACACTGGTg                                                                    >  2:1016466/1‑140 (MQ=255)
                                                                      aaTCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGACAAGCGACCGAACTCTGGTGa                                                                   <  1:45365/140‑1 (MQ=255)
                                                                      aaTCAGACTGCAGTTTTCCATCTCTACATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                                                                   <  1:1661599/140‑1 (MQ=255)
                                                                      aaTCAGACTGCAGTTTACCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                                                                   <  1:2462172/140‑1 (MQ=255)
                                                                            aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAAGGAGGTTTAAAACATCGTGTTCTTTTTCAATTAATGAGTGAAGCGACCGTAGTCTGGTGATATAAt                                                             >  2:1256909/1‑140 (MQ=255)
                                                                            aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGTACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGCCCGAACTATGGTGATATCAt                                                             >  2:2169942/1‑140 (MQ=255)
                                                                            aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAAACGAAAACCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCGGTTCTTTTTCAATTAATGAGAGCAGCGACCGAACTCTGGTGATATCAt                                                             >  2:4943363/1‑140 (MQ=255)
                                                                            aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGCTTTTAATAGAAATACCCCTGTGTGGGGATTTAACGAGCTTTAAAAAAACCTGTTCTTTTTGAAATAAATAGATAAGCGACCGAACGCTGGGGTTATAAt                                                             >  1:4528169/1‑140 (MQ=255)
                                                                            aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTCGTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCGTTAAAACATCCGGTTGTTTTTCAATTAATGTGAGACGCCACCGGACTCTGGTGATATCAt                                                             >  2:4702905/1‑140 (MQ=255)
                                                                                   ttttCCATCTCTTCATAGTCGTTTTCTTTGCCGCTTTTAATCGAAAAACCCGGGGGTGGGGATTGAACGAGCGTTAAAAAATCCTGTTGTTTTTGAATTAATTAGATAATCGACCGCACTCTGGTGATATCATTGTACTc                                                      >  1:3809085/1‑140 (MQ=255)
                                                                                   ttttCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAAACTGGTGATATCATTGAAATc                                                      >  2:3628668/1‑140 (MQ=255)
                                                                                        cATCTCTTCATAGTCGTTTTATTTCCCGATTTTAATCGAAAAACCCGGGTGTGGGGATTGAACGAGCGTTAAAACATCCTGTTGTTTTTCAATTAAATAGAGAACCGACCGAACTCTGGTGATATAATTGAAATACGGCt                                                 >  2:4786363/1‑140 (MQ=255)
                                                                                            tctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                             <  2:2778956/140‑1 (MQ=255)
                                                                                            tctTCATAGTCGTTTTCTTCCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                             <  2:268955/140‑1 (MQ=255)
                                                                                             ctTCATAGTCGTGTTCTTTCCCGATTTTAATGGAAATACCCGGGTGGGGGGCTTGTAAGAGCGTTAAAAAAACCTTTTGTTTTTTAAATAATGAGAGAAGCGACCGAACTCTGGTTTTTTAATGGAAATCCGGCGGGtta                                            >  1:4810301/1‑139 (MQ=255)
                                                                                               tCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAg                                          <  1:5048329/140‑1 (MQ=255)
                                                                                               tCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAg                                          <  1:3327203/140‑1 (MQ=255)
                                                                                                  tAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAATCGACCGAACTCTGGTGATATAATGGAACTCCGGCTGGTTCAGCat                                       >  2:2406334/1‑140 (MQ=255)
                                                                                                  tAGTCGTTTTCTTTCCCGATTGTAATCGAAAAACCCGTGTGTGGGGATTTAACGAGCTTTAAAAAATCCGGTTGTTTTTCAATTAATGAGATAAGCGACCGAACTCTGGTGATATCATGGAACACCGGCGGGTTCAGcct                                       >  2:2589963/1‑138 (MQ=255)
                                                                                                     tCGGTTTCTTTCCCGATTTTAATCCAAATCCCCGTGTGTGGGGATTGTACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAATCGACCGAACTCAGGTTATAACATGGAAAAACGGGTGGTTAAGCataat                                    >  2:2909313/1‑138 (MQ=255)
                                                                                                        ttttATTTCCCGATTTTAATCGAAAAACCCGGGTGTGGGGATTGAAAGAGCGTTAAAAAATACGGTTCTTTTTAAAATAATGAGAGAATCGACCGAACTATGGTGATATAATTGAAATCCGGCAGGTTCAGCATAATGAt                                 >  2:1400357/1‑140 (MQ=255)
                                                                                                            ctttcCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGAttttt                             >  1:5023256/1‑140 (MQ=255)
                                                                                                               tcCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTccg                          >  2:3342854/1‑140 (MQ=255)
                                                                                                                  cGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTccgccg                       >  2:3176053/1‑140 (MQ=255)
                                                                                                                     ttttAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGaaa                    <  2:248665/140‑1 (MQ=255)
                                                                                                                                   cccGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTCATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAAc      <  1:2875321/140‑1 (MQ=255)
                                                                                                                                   cccGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAAc      <  2:4601167/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
AATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGA  >  NZ_CP034943/2502107‑2502381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: