Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,532,199 G→T 5.0% intergenic (+412/+251) EO946_RS13065 → / ← EO946_RS13070 DUF1569 domain‑containing protein/nitronate monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,532,1990GT 5.0% 999.9 / 26.7 333intergenic (+412/+251)EO946_RS13065/EO946_RS13070DUF1569 domain‑containing protein/nitronate monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (144/170);  new base T (9/10);  total (153/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

GCTTGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCA  >  NZ_CP034943/2532061‑2532336
                                                                                                                                              |                                                                                                                                         
gCTTGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCgg                                                                                                                                          <  2:889832/140‑1 (MQ=255)
gCTTGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCgg                                                                                                                                          <  2:438367/140‑1 (MQ=255)
 cTTGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGt                                                                                                                                         >  2:563301/1‑140 (MQ=255)
 cTTGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGt                                                                                                                                         >  2:5113326/1‑140 (MQ=255)
 cTTGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGt                                                                                                                                         >  1:536034/1‑140 (MQ=255)
  ttGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGtt                                                                                                                                        <  2:4318659/140‑1 (MQ=255)
  ttGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGtt                                                                                                                                        <  2:3998311/140‑1 (MQ=255)
       gTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTATTGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTa                                                                                                                                   <  1:952661/140‑1 (MQ=255)
       gTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCTGTTTTCTa                                                                                                                                   >  2:3911686/1‑140 (MQ=255)
        tCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTATac                                                                                                                                  <  1:2468261/140‑1 (MQ=255)
              gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACACCACt                                                                                                                            <  1:529949/140‑1 (MQ=255)
              gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACt                                                                                                                            <  2:5213731/140‑1 (MQ=255)
              gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACt                                                                                                                            <  1:4135596/140‑1 (MQ=255)
              gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACt                                                                                                                            <  1:5113326/140‑1 (MQ=255)
               atgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTa                                                                                                                           <  1:1689914/140‑1 (MQ=255)
                tgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATTGTTTGA‑‑AACCATCGGTTTTCTACAACACTAg                                                                                                                          <  1:4842583/140‑1 (MQ=255)
                tgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAg                                                                                                                          <  1:1401315/140‑1 (MQ=255)
                tgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAg                                                                                                                          <  2:3501568/140‑1 (MQ=255)
                tgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAg                                                                                                                          <  1:1351857/140‑1 (MQ=255)
                  tttattaCCTTCATAAAATGCATCACCCCTTGGGTAATGGTTTGTAATAAATGTTTTTCCTAATTAGGTGATTTTAAATGAGTATGAAAAAAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGc                                                                                                                        <  1:3382476/139‑1 (MQ=25)
                     attaCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                     <  1:19719/140‑1 (MQ=255)
                     attaCATTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                     <  2:2428943/140‑1 (MQ=255)
                     agtaCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTCATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                     <  1:2757016/138‑1 (MQ=255)
                        aCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑TACCATCGGTTTTCTACAACACTAGGCCCTCaa                                                                                                                  <  2:1465922/140‑1 (MQ=255)
                        aCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCaa                                                                                                                  <  2:2838979/140‑1 (MQ=255)
                              aTAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                     >  1:2543563/1‑131 (MQ=255)
                              aTAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                     <  2:2543563/131‑1 (MQ=255)
                              aTAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGt                                                                                                            <  2:3092652/140‑1 (MQ=255)
                                aaaaTGCATCACCCGTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTaa                                                                                                          <  2:1491809/140‑1 (MQ=255)
                                  aaTGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAAt                                                                                                        <  2:3624528/140‑1 (MQ=255)
                                  aaTGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAAt                                                                                                        <  2:2691076/140‑1 (MQ=255)
                                    tGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACGAGGCCATCAACATAGTAAAtac                                                                                                      >  1:4641331/1‑138 (MQ=255)
                                       aTCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCata                                                                                                   >  1:3609698/1‑140 (MQ=255)
                                       aTCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCata                                                                                                   >  1:3483369/1‑140 (MQ=255)
                                          aCCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCa                                                                                                <  2:1166876/140‑1 (MQ=255)
                                              cTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCa                                                                                                >  1:752634/1‑136 (MQ=255)
                                                 gggTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTa                                                                                         >  1:1143741/1‑140 (MQ=255)
                                                   gTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATg                                                                                       <  2:2205586/140‑1 (MQ=255)
                                                     aaTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaa                                                                                     <  1:1805947/140‑1 (MQ=255)
                                                      aTGCTATGTAATAACTGTTTGTCCTAATTAGGTGATTTTCAATGAGTATGAAAAAAGATAAAAAACCGATGGTGTGA‑‑AACCATCGTTTTTCTACAACACTAGGCCCTAAACATAGTAAATCCATATAATTGGTTATGaaa                                                                                    >  1:566725/1‑140 (MQ=255)
                                                      aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                    >  1:2865840/1‑140 (MQ=255)
                                                      aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                    >  1:1581092/1‑140 (MQ=255)
                                                      aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                    >  1:4407686/1‑140 (MQ=255)
                                                      aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                    >  1:3011832/1‑140 (MQ=255)
                                                       tGCTCTGTAATAACGGTTTGTGCTAATGTGGTGATTTTCAATGAGTATGTCACCAGGTAATAATCCGATGGTTTGA‑‑AAGCAGCGGTGTTGGACAGCCCTAGGCCCTCAACCTAGTAAAGCCGGATCCTTGGGTATGAAAg                                                                                   >  2:634915/1‑140 (MQ=255)
                                                       tGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAg                                                                                   >  1:4332349/1‑140 (MQ=255)
                                                            tGTAATATCTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTCGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              <  1:875748/140‑1 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              >  2:3326160/1‑140 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              <  1:3972974/140‑1 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              >  1:424866/1‑140 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              >  2:5031031/1‑140 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              <  1:2193113/140‑1 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              >  1:654809/1‑140 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              <  1:3800785/140‑1 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                              >  2:3236050/1‑140 (MQ=255)
                                                            tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTAATGAAAGCTTGt                                                                              >  2:4244608/1‑140 (MQ=255)
                                                                 taaCTGTTTGTCCTAATGAGGTGATTTTCAATGACTATGAAAACAGATAAAAAACAGATGGTTAGA‑‑AACCATCGGTTTTATACAACACTAGGCCCTCAACAAACTAAATCCATATAATTGTTTAAGAAAGCATGATTTCa                                                                         >  2:3173483/1‑140 (MQ=255)
                                                                     tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACATATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                     <  2:4034673/140‑1 (MQ=255)
                                                                     tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                     <  2:381967/140‑1 (MQ=255)
                                                                     tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                     <  2:373991/140‑1 (MQ=255)
                                                                     tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                     <  1:4594498/140‑1 (MQ=255)
                                                                     tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                     <  2:1582800/140‑1 (MQ=255)
                                                                     tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                     <  1:4061779/140‑1 (MQ=255)
                                                                     tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                     <  1:4550376/140‑1 (MQ=255)
                                                                      gTTTGTCCTAATGCGGTGATTTTCAAGGAGTATGCAAACAGATAAAAAACCGCTGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCAGATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  1:822107/140‑1 (MQ=39)
                                                                      gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACTATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  1:3566018/140‑1 (MQ=255)
                                                                      gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTCGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  1:38607/140‑1 (MQ=255)
                                                                      gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  1:1076824/140‑1 (MQ=255)
                                                                      gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    >  1:619552/1‑140 (MQ=255)
                                                                      gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  1:5071876/140‑1 (MQ=255)
                                                                      gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACGAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  1:3906495/140‑1 (MQ=255)
                                                                      gTTGGTCCTAAGGAGGTGATTGTCATTGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACCCTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  2:4745618/140‑1 (MQ=38)
                                                                       tttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTacac                                                                   <  2:2178142/140‑1 (MQ=255)
                                                                        ttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGt                                                                                                            >  2:4968942/1‑98 (MQ=255)
                                                                        ttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGt                                                                                                            <  1:4968942/98‑1 (MQ=255)
                                                                        ttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACAct                                                                  >  1:993464/1‑140 (MQ=255)
                                                                        ttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAAAACTAGGCCCTCAACATAGTAAAACCATATCATTGGTTATGAAAGCTTGTTTTCATTACAct                                                                  >  1:4008922/1‑140 (MQ=255)
                                                                        ttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTATATCACTAGGCCCTCAATATAGTAAATCTAGCTCATTGGTTATGAAAGCTTATTTTCATTa                                                                      <  2:4571611/140‑1 (MQ=255)
                                                                        ttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTATATCACTAGGCCCTCAATATAGTAAATCTAGCTCATTGGTTATGAAAGCTTATTTTCATTa                                                                      <  2:179100/140‑1 (MQ=255)
                                                                          gTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTATATCACTAGGCCCTCAATATAGTAAATCTAGCTCATTGGTTATGAAAGCTTATTTTCATTaca                                                                    <  1:3150418/140‑1 (MQ=255)
                                                                                     gTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATCGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCAtt                                                     <  2:5186729/140‑1 (MQ=255)
                                                                                     gTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAACTCCATATCATTGGGTATGTACGCTTGTTTTCATTACACTCTGTATACTCAtt                                                     >  1:5033528/1‑140 (MQ=255)
                                                                                     gTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    >  1:1228058/1‑125 (MQ=255)
                                                                                     gTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                    <  2:1228058/125‑1 (MQ=255)
                                                                                          tttCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAACTCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATGTGCCa                                                >  2:2942174/1‑140 (MQ=255)
                                                                                                    tATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCAttt                                                    <  1:2685418/126‑1 (MQ=255)
                                                                                                    tATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCAttt                                                    >  2:39369/1‑126 (MQ=255)
                                                                                                    tATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCAttt                                                    <  1:39369/126‑1 (MQ=255)
                                                                                                    tATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCtat                                      >  2:103582/1‑140 (MQ=255)
                                                                                                            cAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTtat                              <  1:1838599/140‑1 (MQ=255)
                                                                                                              gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTGCAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                            >  2:739946/1‑140 (MQ=255)
                                                                                                              gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAAGACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                            >  2:4668579/1‑140 (MQ=255)
                                                                                                              gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTATGCCCTCAACATATTAAATGCATATCATTGGTTATGAAAGCTTTTTTTCATTACACTCTTTATACTCATTTTCCATTAATTATATACGTTTatat                            >  2:788543/1‑140 (MQ=255)
                                                                                                              gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                            >  2:2392044/1‑140 (MQ=255)
                                                                                                              gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTGCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                            >  1:2138832/1‑140 (MQ=255)
                                                                                                              gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCGACAACACTCGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACACATTTGACATTCATTCTATACGTTTatat                            >  2:3973933/1‑140 (MQ=37)
                                                                                                                     aaaCCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGGCATTAATTCTATACGTTTATATCAATTa                      >  2:3762098/1‑140 (MQ=255)
                                                                                                                          gATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGtt                 >  2:1832339/1‑140 (MQ=255)
                                                                                                                          gATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGtt                 >  2:1932926/1‑140 (MQ=255)
                                                                                                                                            tCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCa  >  2:130924/1‑140 (MQ=255)
                                                                                                                                              |                                                                                                                                         
GCTTGCGGTCCCTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCA  >  NZ_CP034943/2532061‑2532336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: