Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,532,210 A→T 5.1% intergenic (+423/+240) EO946_RS13065 → / ← EO946_RS13070 DUF1569 domain‑containing protein/nitronate monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,532,2100AT 5.1% 1037.1 / 32.5 317intergenic (+423/+240)EO946_RS13065/EO946_RS13070DUF1569 domain‑containing protein/nitronate monooxygenase
Reads supporting (aligned to +/- strand):  ref base A (136/161);  new base T (7/9);  total (145/172)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

Only 100 of 321 total aligned reads displayed.

CTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCAAAAAACACCCTCT  >  NZ_CP034943/2532072‑2532349
                                                                                                                                              |                                                                                                                                           
   gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACt                                                                                                                                         <  1:5113326/140‑1 (MQ=255)
   gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACt                                                                                                                                         <  2:1467120/140‑1 (MQ=255)
   gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACt                                                                                                                                         <  2:5213731/140‑1 (MQ=255)
   gatgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACt                                                                                                                                         <  1:1647671/140‑1 (MQ=255)
    atgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTATGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTa                                                                                                                                        >  2:3293730/1‑140 (MQ=255)
     tgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAg                                                                                                                                       <  1:1351857/140‑1 (MQ=255)
     tgatTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAg                                                                                                                                       <  1:1401315/140‑1 (MQ=255)
       tttattaCCTTCATAAAATGCATCACCCCTTGGGTAATGGTTTGTAATAAATGTTTTTCCTAATTAGGTGATTTTAAATGAGTATGAAAAAAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGc                                                                                                                                     <  1:3382476/139‑1 (MQ=25)
       attattACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGc                                                                                                                                     >  2:19719/1‑140 (MQ=255)
       attattACCTTCATAAAATGCATCACCACTTGGGTAATGCTATTTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATTAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGc                                                                                                                                     <  1:762070/140‑1 (MQ=255)
          attaCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTACTGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                                  <  1:534944/140‑1 (MQ=255)
          attaCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                                  <  1:19719/140‑1 (MQ=255)
           ttaCCTTCATAAAATACATCACCCCTTGGGTAATGCTGCGTAATACCTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTATATCACTAGGc                                                                                                                                     >  1:179100/1‑140 (MQ=17)
           ttaCCTTCATAAAATACATCACCCCTTGGGTAATGCTGCGTAATACCTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTATATCACTAGGc                                                                                                                                     >  1:4571611/1‑140 (MQ=17)
           ttaCCTTCATAAAATACATCACCCCTTGGGTAATGCTGCGTAATACCTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTATATCACTAGGc                                                                                                                                     >  1:218721/1‑140 (MQ=17)
             aCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCaa                                                                                                                               <  2:2838979/140‑1 (MQ=255)
             aCCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCaa                                                                                                                               <  1:636325/140‑1 (MQ=255)
                 tCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATa                                                                                                                           >  2:4611462/1‑140 (MQ=255)
                  cATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAg                                                                                                                          >  1:4077924/1‑140 (MQ=255)
                  cATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAg                                                                                                                          >  2:3888344/1‑140 (MQ=255)
                   aTAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCt                                                                                                                                  >  1:2543563/1‑131 (MQ=255)
                   aTAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGt                                                                                                                         <  2:781282/140‑1 (MQ=255)
                   aTAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGt                                                                                                                         <  2:3092652/140‑1 (MQ=255)
                       aaTGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAAt                                                                                                                     <  2:2691076/140‑1 (MQ=255)
                         tGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTc                                                                                                                                 <  2:4006576/126‑1 (MQ=255)
                         tGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATcc                                                                                                                   >  2:4717780/1‑140 (MQ=255)
                            aTCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCata                                                                                                                >  1:3483369/1‑140 (MQ=255)
                               aCCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGTTTTTCTACAACACTAGGCCCTCAACATAGTAACTCCATATCa                                                                                                             <  2:3595984/140‑1 (MQ=255)
                               aCCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCa                                                                                                             <  2:1187899/140‑1 (MQ=255)
                                      gggTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTa                                                                                                      >  1:1143741/1‑140 (MQ=255)
                                        gTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATg                                                                                                    <  2:2205586/140‑1 (MQ=255)
                                          aaTTCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATAGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaa                                                                                                  <  1:962978/140‑1 (MQ=255)
                                          aaTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaa                                                                                                  <  1:3584141/140‑1 (MQ=255)
                                           aTGCTATGTAATAACTGTTTGTCCTAATTAGGTGATTTTCAATGAGTATGAAAAAAGATAAAAAACCGATGGTGTGA‑‑AACCATCGTTTTTCTACAACACTAGGCCCTAAACATAGTAAATCCATATAATTGGTTATGaaa                                                                                                 >  1:566725/1‑140 (MQ=255)
                                           aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                                 >  1:3999045/1‑140 (MQ=255)
                                           aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                                 >  1:4218014/1‑140 (MQ=255)
                                           aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                                 >  1:994171/1‑140 (MQ=255)
                                           aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                                 >  1:3011832/1‑140 (MQ=255)
                                           aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                                 >  2:4337016/1‑140 (MQ=255)
                                           aTGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGaaa                                                                                                 >  1:5216131/1‑140 (MQ=255)
                                            tGCTCTGTAATAACGGTTTGTGCTAATGTGGTGATTTTCAATGAGTATGTCACCAGGTAATAATCCGATGGTTTGA‑‑AAGCAGCGGTGTTGGACAGCCCTAGGCCCTCAACCTAGTAAAGCCGGATCCTTGGGTATGAAAg                                                                                                >  2:634915/1‑140 (MQ=255)
                                                 tGTAATCACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAATTCCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  1:4602384/1‑140 (MQ=255)
                                                 tGTAATATCTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTCGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           <  1:875748/140‑1 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  2:3236050/1‑140 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           <  1:3972068/140‑1 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  2:1390348/1‑140 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  2:2389736/1‑140 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           <  1:4251013/140‑1 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  1:2218751/1‑140 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  2:5031031/1‑140 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  1:1820691/1‑140 (MQ=255)
                                                 tGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCAGTGGTTATGAGAGCTTGt                                                                                           >  1:2925388/1‑140 (MQ=255)
                                                 tGTAATAACTGTTTGTCATAATGAGGTGATTTACAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACAAAGTAAATCCAAATCATTTGTTATGAAATCTTGt                                                                                           >  1:4056163/1‑140 (MQ=255)
                                                 tGTAATAACTGGTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑CACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGt                                                                                           >  1:2853158/1‑140 (MQ=255)
                                                      taaCTGTTTGTCCTAATGAGGTGATTTTCAATGACTATGAAAACAGATAAAAAACAGATGGTTAGA‑‑AACCATCGGTTTTATACAACACTAGGCCCTCAACAAACTAAATCCATATAATTGTTTAAGAAAGCATGATTTCa                                                                                      >  2:3173483/1‑140 (MQ=255)
                                                          tttttGTCCTAATGAGCTGTTTTTCAATGACTATGAAAACAGATAAAAAACAGATTGTTTGA‑‑AACCATCTGTTTTATACATCATTTGTCAATCAACTTATTAAATCTTTATTATTTTTTATGATAGCTTGTTTTGATTac                                                                                  <  2:4194374/138‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGTTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  2:3762282/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  2:373991/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  1:3888344/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  2:2418859/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  1:3779150/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  2:381967/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  2:1582651/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  1:4500244/140‑1 (MQ=255)
                                                          tGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  1:1902892/140‑1 (MQ=255)
                                                           gTTTGTCCTAATGCGGTGATTTTCAAGGAGTATGCAAACAGATAAAAAACCGCTGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCAGATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 <  1:822107/140‑1 (MQ=39)
                                                           gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTCGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 <  1:38607/140‑1 (MQ=255)
                                                           gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 <  1:3919799/140‑1 (MQ=255)
                                                           gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 >  2:1159415/1‑140 (MQ=255)
                                                           gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 <  1:1076824/140‑1 (MQ=255)
                                                           gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 <  2:536034/140‑1 (MQ=255)
                                                           gTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 <  1:5071876/140‑1 (MQ=255)
                                                            tttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTacac                                                                                <  1:502237/140‑1 (MQ=255)
                                                            tttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTacac                                                                                <  2:4565983/140‑1 (MQ=255)
                                                            tttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTacac                                                                                <  2:2178142/140‑1 (MQ=255)
                                                            tttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTacac                                                                                <  1:608392/140‑1 (MQ=255)
                                                             ttGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGt                                                                                                                         <  1:4968942/98‑1 (MQ=255)
                                                               gTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTATATCACTAGGCCCTCAATATAGTAAATCTAGCTCATTGGTTATGAAAGCTTATTTTCATTaca                                                                                 <  1:3150418/140‑1 (MQ=255)
                                                                 ccTCATGAGGAGATTTTCAATGAGTATGACAACAAATAAAAAAACCGATGGTTTGTACCATCGATCTGTTTTCTAGATCACTAGGCCCTCAATATAGTAAATCTAGCTCATTGGTTATGAAAGCTTATTTTCATTacaca                                                                               <  2:3399202/140‑2 (MQ=255)
                                                                          gTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATCGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCAtt                                                                  <  2:5186729/140‑1 (MQ=255)
                                                                          gTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTaca                                                                                 >  1:1228058/1‑125 (MQ=255)
                                                                          gTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTac                                                                                  <  2:396515/124‑1 (MQ=255)
                                                                           tGAATTTCAATGAGTATTAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTACGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCAttt                                                                 <  2:3976767/140‑1 (MQ=255)
                                                                               tttCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAACTCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATGTGCCa                                                             >  2:2942174/1‑140 (MQ=255)
                                                                                        gTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCta                                                    <  2:180080/140‑1 (MQ=255)
                                                                                        gTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCta                                                    <  2:5098425/140‑1 (MQ=255)
                                                                                         tATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCAttt                                                                 <  1:2685418/126‑1 (MQ=255)
                                                                                         tATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCtat                                                   >  1:4745562/1‑140 (MQ=255)
                                                                                                   gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTGCAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                                         >  2:739946/1‑140 (MQ=255)
                                                                                                   gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                                         >  2:877219/1‑140 (MQ=255)
                                                                                                   gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                                         >  2:481928/1‑140 (MQ=255)
                                                                                                   gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                                         >  2:1265675/1‑140 (MQ=255)
                                                                                                   gATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTatat                                         >  2:2550330/1‑140 (MQ=255)
                                                                                                               gATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGtt                              >  2:1832339/1‑140 (MQ=255)
                                                                                                               cttGGTTTTA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGtt                              <  1:4338568/138‑1 (MQ=255)
                                                                                                                                 tCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCa               >  2:1610613/1‑140 (MQ=255)
                                                                                                                                     ttttCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCaaaaa           <  1:3682178/140‑1 (MQ=255)
                                                                                                                                       ttCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCAAAAAac         <  1:2442057/140‑1 (MQ=255)
                                                                                                                                          taaaacaCTAGGCCCTCAACATATTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCAAAAAACAcc      <  2:4135867/137‑1 (MQ=255)
                                                                                                                                          tACAACACGAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCAAAAAACAcc      <  1:465620/140‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
CTCGATGATTATTACCTTCATAAAATGCATCACCCCTTGGGTAATGCTATGTAATAACTGTTTGTCCTAATGAGGTGATTTTCAATGAGTATGAAAACAGATAAAAAACCGATGGTTTGA‑‑AACCATCGGTTTTCTACAACACTAGGCCCTCAACATAGTAAATCCATATCATTGGTTATGAAAGCTTGTTTTCATTACACTCTTTATACTCATTTGCCATTAATTCTATACGTTTATATCAATTATGGTTAAAAAATGGTGAGCAAAAAACACCCTCT  >  NZ_CP034943/2532072‑2532349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: