Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,910,843 G→C 6.4% intergenic (‑43/‑147) EO946_RS14985 ← / → EO946_RS14990 MFS transporter/LMBR1 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,910,8430GC 6.4% 515.7 / 3.5 213intergenic (‑43/‑147)EO946_RS14985/EO946_RS14990MFS transporter/LMBR1 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (108/82);  new base C (2/16);  total (110/103)
Fisher's exact test for biased strand distribution p-value = 2.62e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

GATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTAGAAAAAGGGG  >  NZ_CP034943/2910704‑2910982
                                                                                                                                           |                                                                                                                                           
gATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACg                                                                                                                                             <  2:5096479/140‑1 (MQ=255)
 aTTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGa                                                                                                                                            <  1:3491489/140‑1 (MQ=255)
 aTTATGGATATAAATAGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATTATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGa                                                                                                                                            <  2:1035979/140‑1 (MQ=255)
   tATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACAGTCCAATGATTAAAATTTTATGAGCACGCGGCATACTAATCCCCCTTTCGTCCGTAAAAAAGCTCATGAAACAAACGaac                                                                                                                                          >  1:959/1‑139 (MQ=255)
           taAATCGTATTCAGCGGCCATAAAAAATAAGCACCCGTCACATTTATAAACTTTCAATTTATTAAAATTTTAAGAGCACGCGGCATAATAATCCCCCTTTCTTCCCTAAAAAAGCTAATTAAAAAAACTAAACCTaaaaa                                                                                                                                  <  2:2793066/140‑1 (MQ=255)
           taAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTaaaaa                                                                                                                                  >  1:1478611/1‑140 (MQ=255)
               tattATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAAAATTCCAATGATTAAAATTTTAACAGAACGCGGCATAATAATCCCCCTTTCTTCCATAAAAAAGCTCATTAAAAAAACGAAACCTAAAAACGGc                                                                                                                              <  2:1330459/137‑1 (MQ=255)
                 gTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTc                                                                                                                            >  1:4334534/1‑140 (MQ=255)
                  tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                                                                           >  1:4283407/1‑140 (MQ=255)
                  tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                                                                           >  1:1052952/1‑140 (MQ=255)
                  tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                                                                           >  1:256862/1‑140 (MQ=255)
                  tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                                                                           >  2:1661320/1‑140 (MQ=255)
                  tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                                                                           >  1:190995/1‑140 (MQ=255)
                  tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcc                                                                                                                           >  2:2131035/1‑140 (MQ=255)
                  tATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATACCCCTTTATTCCCTCAAAAAGCTCATGAAAAAAACGAAACCAAAAAACGGCTcc                                                                                                                           >  2:3035745/1‑140 (MQ=255)
                   aTTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTcag                                                                                                                          >  2:1225637/1‑138 (MQ=255)
                       aGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACg                                                                                                                      >  2:2019883/1‑140 (MQ=255)
                         cGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGaa                                                                                                                    >  2:3617389/1‑140 (MQ=255)
                         cGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCACCCTTTCTTCCCTAAAAAAGATCATGAAAAAAACGAAACCTAAAAACTGCAGCGTACGaa                                                                                                                    >  2:2969197/1‑140 (MQ=255)
                            ccATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTACGTACGAATCg                                                                                                                 >  2:4765798/1‑140 (MQ=255)
                             cATAAAAAACACGCACCCTTAAATTTGATAAACATTCAAATTATTAAAATTTTAAGAGCACGCGGAATAATAAGCCCCCTTTCTCCCAAAAAAAAGCACATCAAAAAAACCAAACCCAAACACGGCTCCGTACGAATCGa                                                                                                                <  1:926268/140‑1 (MQ=255)
                             cATAAAAAACACCCCCCCGACACATTGAAAAACATACCACTTATAAAAATTTCACGCGCCCGCGGAATACTACGCCCCCTTACTGCCCTAAAAAAGCTCATCAAAAAAACGAAACCTACACACGGCTCCGTACGAATCGa                                                                                                                <  1:3923396/140‑1 (MQ=255)
                              aTAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAg                                                                                                               >  2:191283/1‑140 (MQ=255)
                              aTAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAg                                                                                                               >  1:2004119/1‑140 (MQ=255)
                              aTAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAg                                                                                                               <  2:289139/140‑1 (MQ=255)
                               tAAAAAAGACCCACCCGACACATTGATAAACATTCCAATTATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTACTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGt                                                                                                              <  2:1306904/140‑1 (MQ=255)
                                      gaagCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATa                                                                                                       >  2:3734864/1‑140 (MQ=255)
                                      gaagCACCCGTCAAATTTATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATa                                                                                                       <  2:1865939/140‑1 (MQ=255)
                                       aagCACCCGTCACCTGGATAAACATGCCAATGATTAAAATTTTAATAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAg                                                                                                      <  2:2228115/140‑1 (MQ=255)
                                       aagCACCCGTCACATTGATAAACATGCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTGCTTCCGTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAg                                                                                                      <  2:1555868/140‑1 (MQ=255)
                                       aagCACCCGTCAAATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTGCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAg                                                                                                      <  2:2186740/140‑1 (MQ=255)
                                       aaccACCCGTAACATTGATAAACATTCAAATGATTAAAATTTTAAGACCACGCGGCATACTAAGCCCCCTTTCTCCCCAAAAAAAGCTCATGAAAAAAACGAAACCAAAAAACGGCTCCGTACGAATCGAGTTATCATAg                                                                                                      <  1:77018/137‑1 (MQ=255)
                                             ccGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCAcatt                                                                                                <  2:4259234/140‑1 (MQ=255)
                                             ccGTCACATAGATAAACATTCCAATGATTAAAATTTTAAGAGCCCGCGGCAAACTAATCCCCCTAGCTCCCCTAAAAAAGCTCATCAAAAAAACGAAACCCAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcatt                                                                                                <  1:1355734/140‑1 (MQ=255)
                                             ccGTAACATTGATAAACATTCCAATTATTAAATTTTTAAGAGACCGGGGAAAACTAAGCCCCCTTGCTTCCATAAAAAAGAACAACAAAAAAACCAAACATAAACACGGCTCCGTACGAATCGAGTTATCATAGCAcatt                                                                                                <  1:4589481/140‑1 (MQ=255)
                                               ttCACATTCATAAACATTCCAATTATTAAAATTTTAAGACCCCGCGGCATACAAATCCCCCTTTCTTCCCTAAAAAAGCTCATCAAAAAAACGAAACCAAAACACGGCTCCGTACGAATCGAGTTATCATAGCAcattca                                                                                              <  1:4692501/139‑1 (MQ=255)
                                               gtatcaTTGATAAACATTCCATGGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACTAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcattca                                                                                              <  2:1616574/136‑1 (MQ=255)
                                               gacacaTTCATAAACATTCAATTTTTTAAAATTTAAAGACCCCGGGGAATACTAAGCCCCCTTCCTGCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCAcattca                                                                                              <  1:3368632/138‑1 (MQ=255)
                                               gTCACATTTATAAACATTCCACTGATTAAAATTTTACGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCAGGAAAAAAACGAACCCAAAACACGGCTCCGTACGAATCGAGTTATCATAGCAcattca                                                                                              <  1:1289299/140‑1 (MQ=255)
                                               gTCACATTGATAAACATCCCAATGATTAAAATTTTAAGCGCACGGGGCATACTAATCCCCCTTGCTGCCCTAAAAACGCTCATGAAAAAAACGAACCCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcattca                                                                                              <  1:3747254/140‑1 (MQ=255)
                                               ctCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATTAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcattca                                                                                              <  2:1704889/139‑1 (MQ=255)
                                               cccacaTGCATAAACATTCCAATTATTAAAATTTAAAGACCCCCCGCCATACTAAGCCCCCTTCCTCCCCTAAAAAAGCACATCAAAAAAACGACACCTACACACGGCTCCGTACGAATCGAGTTATCATAGCAcattca                                                                                              <  1:2144654/138‑1 (MQ=255)
                                                 cacaTTGATAAACATTCCAATGATTAAAATTTTAAGAGCCCGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcattcatt                                                                                            <  2:4559469/140‑1 (MQ=255)
                                                 cacaTTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCAcattcatt                                                                                            >  2:324185/1‑140 (MQ=255)
                                                    aTTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATAATAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTa                                                                                         <  2:2502743/140‑1 (MQ=255)
                                                      tGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAAACTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtc                                                                                       >  1:2339327/1‑140 (MQ=255)
                                                       gATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtct                                                                                      >  1:1902676/1‑140 (MQ=255)
                                                       gATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtct                                                                                      >  1:1310313/1‑140 (MQ=255)
                                                       gATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtct                                                                                      >  1:2286355/1‑140 (MQ=255)
                                                         tAAACTTTCTAATTTTTAAATTTTTACGCCCACGGGTCATACTAATCCCCATTCCTTCCATAAAAATGCTCATTAAAAAAACGAACCGTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtctct                                                                                    <  1:1017130/140‑1 (MQ=255)
                                                         tAAACTTTCAAATTATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTAATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtctct                                                                                    <  2:4637624/140‑1 (MQ=255)
                                                         tAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtctct                                                                                    <  2:4634656/140‑1 (MQ=255)
                                                         tAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtctct                                                                                    <  2:3366204/140‑1 (MQ=255)
                                                         tAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGATCTTGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTAtctct                                                                                    <  1:5212117/140‑1 (MQ=255)
                                                            aCATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACTGCTCCGTAAGAATCGAGTTATCATAGAACATTAATTCTATATCTCTg                                                                                 >  2:906373/1‑140 (MQ=255)
                                                                tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtctc                                                                             >  1:3016139/1‑140 (MQ=255)
                                                                tCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGtctc                                                                             >  1:1141669/1‑140 (MQ=255)
                                                                  cAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTcac                                                                           >  1:939330/1‑140 (MQ=255)
                                                                  cAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTcaa                                                                           >  2:873688/1‑139 (MQ=255)
                                                                  cAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGATCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTcac                                                                           >  2:1881345/1‑140 (MQ=255)
                                                                    aTGATAAAAATTTAAACACCACGCGGCAAACTAACCCCCCTTTCGTCCCAAAAACCGCTCATGAAAAACACCAAACCTCACAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAt                                                                         <  1:1768206/140‑1 (MQ=255)
                                                                      taTTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTGCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAttt                                                                       <  2:4319277/139‑1 (MQ=255)
                                                                      gATTAAAATTTAAAGAGCACGCGGAATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCTTCAAAAAAACCAACCCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAttt                                                                       <  1:3467008/140‑1 (MQ=255)
                                                                       aTTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAtttt                                                                      >  1:121226/1‑140 (MQ=255)
                                                                        ttAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCGAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACAttttt                                                                     >  1:4635065/1‑140 (MQ=255)
                                                                          aaaaTTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCa                                                                   >  2:135475/1‑140 (MQ=255)
                                                                           aaaTTTTAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAGACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAAc                                                                 <  2:1791907/140‑1 (MQ=255)
                                                                            aaTTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAAc                                                                 <  2:2593167/140‑1 (MQ=255)
                                                                            aaTTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAAc                                                                 <  2:3975487/140‑1 (MQ=255)
                                                                                 tAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACtgatg                                                            >  2:567989/1‑140 (MQ=255)
                                                                                 tAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAAACTAAAAACGGCTCCGTACGAATCGAGATATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTAAACtgatg                                                            >  2:3674766/1‑140 (MQ=255)
                                                                                      gCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAAACGAGTTATCATATCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGAAGAGCGt                                                       >  2:853639/1‑140 (MQ=255)
                                                                                              cATCCTAATCCCCCTCCCGGCCCCAACAACGCACACCAAAAAAACGAACCCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAt                                               <  1:282702/140‑1 (MQ=255)
                                                                                              cATACTAACCCCCCTTTCTTCCCTAACAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAt                                               <  2:2339327/140‑1 (MQ=255)
                                                                                                tccTAATCCCCCTTTCGTCCCTAAAAAAGCACATTAAAAAAACCAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCt                                             <  1:1617035/138‑1 (MQ=255)
                                                                                                aaCTCATCCCCCTTTCTTCCCTAAAAAACACCATGAAAAAAACGAACCCTACACACCGCTCCGTACGAATCGAGTTATCATAGCACATTCAGTCTATCTCTCGGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCt                                             <  1:885476/139‑1 (MQ=255)
                                                                                                   aactCCCCCTTTCTCCCCTAAAAAACCTCATCAAAAAAACTAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAAc                                          <  1:682387/137‑1 (MQ=255)
                                                                                                      tCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTTATGAGCGTGACAAAATCTAACaaa                                       >  2:4777070/1‑140 (MQ=255)
                                                                                                        ccccTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAata                                     >  2:124045/1‑140 (MQ=255)
                                                                                                        ccccTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAata                                     >  2:4181846/1‑140 (MQ=255)
                                                                                                        ccccTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAata                                     >  1:289593/1‑140 (MQ=255)
                                                                                                        ccccTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAata                                     >  2:3830164/1‑140 (MQ=255)
                                                                                                        ccccTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAata                                     >  1:5061290/1‑140 (MQ=255)
                                                                                                        ccccTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAAAAAata                                     >  2:600655/1‑140 (MQ=255)
                                                                                                          ccTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATAtt                                   >  2:798826/1‑140 (MQ=255)
                                                                                                           cTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAACAAATCTAACAAATATTg                                  >  1:1051498/1‑140 (MQ=255)
                                                                                                                 tcCCTGAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAt                                               >  1:3101875/1‑121 (MQ=255)
                                                                                                                 tcCCTGAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAAt                                               <  2:3101875/121‑1 (MQ=255)
                                                                                                                 tcCATAAAAAAGCTCATCAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTaaa                            <  1:3332381/140‑1 (MQ=255)
                                                                                                                  cccTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAAGAAATATTGTTTaaaa                           >  2:4040738/1‑140 (MQ=255)
                                                                                                                  cccTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTAAATCTCTCTTTCTCACATTTTTCAACTGATGATCGTGAAAAAATCTAAAAAATATTTTTTaaaa                           >  2:2918981/1‑140 (MQ=255)
                                                                                                                        aaaaGCTCATGAAAAAAACGAAGCCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTa                     >  1:801582/1‑140 (MQ=255)
                                                                                                                             cTCATTAAAAAAACCAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAAtc                <  1:1437184/140‑2 (MQ=255)
                                                                                                                                 ttaaaaaaaCGAAACCTAACAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTa            <  2:2642251/138‑1 (MQ=255)
                                                                                                                                 tGAAAAAAACGAAACCTAAAAACGGCTCAGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTa            <  2:4460565/140‑1 (MQ=255)
                                                                                                                                      aaaaCGAAACCTAACCACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTAGaaaa       <  1:2362123/140‑1 (MQ=255)
                                                                                                                                      aaaaCGAAACCTAAACACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTAGaaaa       <  1:4772174/140‑1 (MQ=255)
                                                                                                                                      aaaaCGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTAGaaaa       <  1:3706410/140‑1 (MQ=255)
                                                                                                                                      aaaaCCAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTAGaaaa       <  1:2949385/140‑1 (MQ=255)
                                                                                                                                           gAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTAGAAAAAgggg  <  2:4635065/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GATTATGGATATAAATCGTATTCAGCGGCCATAAAAAAGAAGCACCCGTCACATTGATAAACATTCCAATGATTAAAATTTTAAGAGCACGCGGCATACTAATCCCCCTTTCTTCCCTAAAAAAGCTCATGAAAAAAACGAAACCTAAAAACGGCTCCGTACGAATCGAGTTATCATAGCACATTCATTCTATCTCTCTGTCTCACATTTTTCAACTGATGAGCGTGAAAAAATCTAACAAATATTGTTTAAAAAATGTAAAATAAGTAGAAAAAGGGG  >  NZ_CP034943/2910704‑2910982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: