Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,962,633 G→T 5.8% V313L (GTA→TTA)  EO946_RS15265 → glycosyltransferase family 4 protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,962,6330GT 5.8% 850.2 / 9.4 301V313L (GTA→TTA) EO946_RS15265glycosyltransferase family 4 protein
Reads supporting (aligned to +/- strand):  ref base G (145/130);  new base T (2/24);  total (147/154)
Fisher's exact test for biased strand distribution p-value = 5.20e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

CATTCCAAATCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACAAAAAACGGCCGGCACAATGTAGAATC  >  NZ_CP034943/2962494‑2962769
                                                                                                                                           |                                                                                                                                        
cATTCCAAATCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTg                                                                                                                                          >  1:1241397/1‑140 (MQ=255)
cATTCCAAATCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTg                                                                                                                                          >  1:3302955/1‑140 (MQ=255)
  ttCCAAATCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTa                                                                                                                                        <  1:1821411/140‑1 (MQ=255)
   tCCAAATCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTGAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTac                                                                                                                                       >  1:648053/1‑139 (MQ=255)
      aaaTCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAAAACCTCTCCCGCCTGAATTATGAACCAATGGCGGCCGGAAAGCCCTTAATAACCACAAACAGGGGCGGAAACGGAGACGTTTTAAAac                                                                                                                                    >  1:4341849/1‑140 (MQ=255)
        aTCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAGACGGAGAGGTTGTAAAacac                                                                                                                                  >  1:4853493/1‑140 (MQ=255)
        aTCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAacac                                                                                                                                  >  2:929287/1‑140 (MQ=255)
         tctcTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACg                                                                                                                                 <  2:4957960/140‑1 (MQ=255)
                     ggCAGCTGTATTTGTGTGCAGCTCTCAGTGGAATGAAACCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCATTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGcc                                                                                                                     <  2:1533615/140‑1 (MQ=255)
                     ggCAGATGTATTTGTGTGCAGCTCTCAGGGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGCCAACGGcc                                                                                                                     <  2:2185961/140‑1 (MQ=255)
                     ggCAGATGTATTTGTGTGCAGCGCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGACGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCACCGGcc                                                                                                                     <  2:4907255/140‑1 (MQ=255)
                              atgtgtgtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCg                                                                                                            <  2:1817707/137‑1 (MQ=255)
                               tttGTGTGCAGCTCTCAGTGGAATGATCCCCTCGCCCGCGTGAATTAGGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCCTCGa                                                                                                           <  2:3847779/140‑1 (MQ=255)
                                   tgtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCACTGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGc                                                                                                       <  1:1387361/140‑1 (MQ=255)
                                   tgtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGc                                                                                                       <  1:114056/140‑1 (MQ=255)
                                   tgtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGc                                                                                                       <  1:3117206/140‑1 (MQ=255)
                                   tgtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGc                                                                                                       <  1:2286137/140‑1 (MQ=255)
                                   tgtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAGTCAACGGCCTTGTCATCGACAGCt                                                                                                      <  1:245933/140‑1 (MQ=255)
                                    gtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACCAACCGGGGCGGAACCGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACCGCt                                                                                                      >  2:2587790/1‑140 (MQ=255)
                                    gtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCt                                                                                                      <  2:718811/140‑1 (MQ=255)
                                    gtgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGCAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCt                                                                                                      <  2:3410071/140‑1 (MQ=255)
                                     tgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCACTGGCGGCCGGAACGCCCTTAATTACCCCAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTa                                                                                                     >  2:1748428/1‑140 (MQ=255)
                                     tgCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGACCGCCCTTAATTACCACAAACCGGGGCGGAACCGGAGAGGGTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTa                                                                                                     >  1:333283/1‑140 (MQ=255)
                                      gCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTac                                                                                                    >  1:1033369/1‑140 (MQ=255)
                                          ctctCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGTCAGCTacaaca                                                                                                <  1:3968467/140‑1 (MQ=255)
                                          ctctCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTacaaca                                                                                                <  1:3714713/140‑1 (MQ=255)
                                          ctctCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTacaaca                                                                                                <  1:4957745/140‑1 (MQ=255)
                                            ctcAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACaaa                                                                                              <  1:3444001/140‑1 (MQ=255)
                                            ctcAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACaaa                                                                                              <  1:458706/140‑1 (MQ=255)
                                            ctcAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACaaa                                                                                              <  2:3302955/140‑1 (MQ=255)
                                               aGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGTGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAAccc                                                                                           >  2:1914903/1‑140 (MQ=255)
                                                    aaTGACCCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTc                                                                                      >  2:2969430/1‑140 (MQ=255)
                                                    aaTGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTc                                                                                      >  2:2643616/1‑140 (MQ=255)
                                                    aaTGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTc                                                                                      >  2:3047205/1‑140 (MQ=255)
                                                    aaTGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTc                                                                                      >  1:5181915/1‑140 (MQ=255)
                                                    aaTGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTc                                                                                      >  2:1700810/1‑140 (MQ=255)
                                                    aaTGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTGAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTc                                                                                      >  2:1394096/1‑140 (MQ=255)
                                                      tGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACg                                                                                                                                 <  2:1598266/95‑1 (MQ=255)
                                                         aCCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTg                                                                                 >  2:4974018/1‑140 (MQ=255)
                                                         aCCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTg                                                                                 >  2:1225709/1‑140 (MQ=255)
                                                            ccTCGCCCGCGTTAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCGTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGa                                                                              <  2:2825255/140‑1 (MQ=255)
                                                            ccTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGGCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTgaga                                                                              >  1:4753567/1‑140 (MQ=255)
                                                            ccTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTTTAAAACACGAAGTAAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGa                                                                              <  2:2016244/140‑1 (MQ=255)
                                                            ccTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGa                                                                              <  2:3622194/140‑1 (MQ=255)
                                                            ccTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGa                                                                              <  2:149220/140‑1 (MQ=255)
                                                            ccTCGCCCGCGTGAATTATGAAGAAATGGCGGCCGGAACGCCATTAATTACCACAACCCGGGGCGGAAACGTAGAGGTTGTAAAACACGAAGTCAACGGCCTGGTCATCGACAGCTACAACAAACCCACTTCATTTGCGa                                                                              <  2:608164/140‑1 (MQ=255)
                                                             cTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGaa                                                                             >  1:1451941/1‑140 (MQ=255)
                                                             cTCCCCCGCGGGAATTATGCAGCAATGGCGGCCGGACCGCCCTTCATTACCACAAACCGGGGCGGAAAGGGAGAGGTTGTAAAACTCGAAGTCACCGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGaa                                                                             >  2:510631/1‑140 (MQ=255)
                                                                   cgcgTTAATTATGAAGCAATGGCGGCCGGACCGCCCTTATTTACCACAAACCGGGGCGGAAACGGTGAGGTTGTAAAACACGAAGTCACCGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAAt                                                                       <  1:1707812/140‑1 (MQ=255)
                                                                   cgcgTGAATTATGAAGAAATGGCGGCCGTAACGCCCTTATTTACCACAAACCGGGGCGTAAACGGAGTGTTTTTAAAACACTAATTCAACGGCCTTGTCATCGACAGCTACACCAACCCCTCTTAATTTGCTAAAGAAAt                                                                       <  1:1562322/140‑1 (MQ=255)
                                                                     cgTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCg                                                                     >  2:626856/1‑140 (MQ=255)
                                                                     cgTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCg                                                                     >  1:300963/1‑140 (MQ=255)
                                                                       tGAATTATGAAGCATTGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGGGTAAACGGCGTGGTTTTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGAc                                                                   <  1:4503664/140‑1 (MQ=255)
                                                                       tGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACACACCGGGGCGGAAACGGAGAGTTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTGGGTAAAGAAATCgcc                                                                   <  2:316819/140‑3 (MQ=255)
                                                                       tGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGAc                                                                   <  2:4340909/140‑1 (MQ=255)
                                                                       tGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTAATTTGCGAAAGCAATCGAc                                                                   <  2:2433680/140‑1 (MQ=255)
                                                                       ggAATTACGAACCACCGGCGGCCGGAACGCCATTAATTACCACAAACCGGGGCGGAAACGTAGATGTTGTAAAACCCGAAGTCAACGCCCTTGTCTTCGACAGCTACAACAAACCCCCTACATTGGCGAAAGCAATCGAc                                                                   <  1:851300/139‑1 (MQ=255)
                                                                       acagttATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAACCCGGGGCGGAAACGGAGTGGTTTTAAAACACGAAGTCAACGGCTTTGTAATCGACAGATAAAACAAACCCTCTTCATTTGCGAAAGCAATCGAc                                                                   <  1:3588396/136‑1 (MQ=255)
                                                                         agttATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCtt                                                                                                                   >  1:1670086/3‑90 (MQ=255)
                                                                         agttATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCtt                                                                                                                   <  2:5005259/88‑1 (MQ=255)
                                                                         agttATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCtt                                                                                                                   >  1:5005259/3‑90 (MQ=255)
                                                                         aaTTATGAAGCAATGGCGGCCGGACCGCCCTTAATTACCACACCCCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCGTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACag                                                                 <  1:427856/140‑1 (MQ=255)
                                                                          aTTATGAAGCAATGGCGGCCGGAACGCCATTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACTAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACaga                                                                <  2:781932/140‑1 (MQ=255)
                                                                           ttATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGc                                                                                                       >  1:2886217/1‑100 (MQ=255)
                                                                           ttATGAAGCAAGGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACagag                                                               <  2:557297/140‑1 (MQ=255)
                                                                            tATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGc                                                              <  2:4148916/140‑1 (MQ=255)
                                                                              tGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGccg                                                            >  2:4037844/1‑139 (MQ=255)
                                                                                aaGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCttt                                                          >  1:3921416/1‑140 (MQ=255)
                                                                                 aGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACCAAGTCAACGGCATTGTCATCGACAGCTACAACAACCCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTa                                                         <  2:3587978/140‑1 (MQ=255)
                                                                                 aGCAAGGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGTTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTa                                                         <  1:1421747/140‑1 (MQ=255)
                                                                                  gCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAACCGGAGAGGTTGTAAAACACGACGTCAAGGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAATGCAATCGACAGAGCCTTTAc                                                        <  1:258025/140‑1 (MQ=255)
                                                                                   cactGGCGGCCGGAACGCCCTTCATTACCCCACCCCGCGGCGCAAACGCAGCGGTTGTCAAACACGAAGTCAACGGCCTCGTCATCGACAGCTACAACAAACCCTCGTCATTTGCGAAAGCAATCTACAGAGCCTTTACa                                                       <  2:3690605/137‑1 (MQ=255)
                                                                                   cAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGcc                                                                                                                     >  2:813349/1‑78 (MQ=255)
                                                                                       ggcggcCGGAACGCCCTTAATTACCAAAAACCGGGGCGGAAACGGAGAGGTTTTAAAACACGAAGTAAACGGCCTTGTCATCGACAGATAAAACAAACCCTCTTCATTTGCTAAAGCAATCGACAGAGCCTTTACAGAcc                                                   <  1:2149930/140‑1 (MQ=255)
                                                                                           gcCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACCGAGCCTGTACAGACCAAGa                                               >  2:4685422/1‑140 (MQ=255)
                                                                                             cGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGcc                                                                                                                     <  1:4175923/68‑1 (MQ=255)
                                                                                             cGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTGCATTTGCGAAAGCAAGCGACAGAGCCTTTACAGACCAAGAAt                                             >  2:390583/1‑140 (MQ=255)
                                                                                                         aaTTACCACAAACGGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTCCAACAATCCCTGTTGATTTGCGAAAGCAATCGTCAGAGCCTTTACAGACCAAGTGTTAATGTACcaaa                                 >  2:4477007/1‑140 (MQ=255)
                                                                                                         aaTTACCACAAACCGGGGCGGAAACGGAGAGGTTTTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACaaaa                                 <  1:305023/140‑1 (MQ=255)
                                                                                                              ccACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAAAAAAATcaca                            >  2:4228893/1‑140 (MQ=255)
                                                                                                               cacaAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACaa                           >  2:24906/1‑140 (MQ=255)
                                                                                                               cacaAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACaa                           >  2:1253782/1‑140 (MQ=255)
                                                                                                               cacaAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACaa                           >  2:3208899/1‑140 (MQ=255)
                                                                                                               cacaAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGACAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTACATTAGCGAAAGCAATCGACAGACCCTATACAGAACAAGAATTAATGAACAAAATCACaa                           >  2:4795089/1‑140 (MQ=255)
                                                                                                                 caAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGAcc                                                   >  2:1340267/1‑114 (MQ=255)
                                                                                                                 caAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACaaaa                         >  1:2934102/1‑140 (MQ=255)
                                                                                                                  aaaCCGGGGCGGAAAGGGAGAGGTTGAAAAACAGGAAGTCACCGGCATCGTCATCTACACCAACAACAAACCCTCTTCATTTGCGAAAGAAATCTAAAGAGACTGTACACACCAAGAATTAATGAAAAAAAGCAcaacaa                        <  2:3975168/140‑1 (MQ=255)
                                                                                                                   aaCCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACaaaaaa                       >  1:1976327/1‑140 (MQ=255)
                                                                                                                    aCCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGc                                                              <  1:792339/100‑1 (MQ=255)
                                                                                                                    aCCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGc                                                              >  2:792339/1‑100 (MQ=255)
                                                                                                                       ggggCGGAAACGGTGTGGTTTTAAAACACGAAGTAAACGGCCTTGTCATCGACAGCTACAACAAACCCTTTTCATTTGCTAAAGCAATCGACAGAGCCTTTACAGACCAATAATTAATGAACAAAATCACAAAAAAcggc                   <  2:478641/140‑1 (MQ=255)
                                                                                                                            gtaaaCGGAGAGGTTGTAAAACACGAAGTAAACGGCCTTTTCATCTACAGCTACAACAAACCCTCTTAATTTGCGAAAGCAATCGACTGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACAAAACACGGCCGGca              <  2:2096046/138‑1 (MQ=255)
                                                                                                                            ggAAACGGAGTGTTTGTAAAACACGATGTCAACGGCCTTGTCATGGACAGCTACAAAAAACCCTCTTAATTTGCGAAAGAAATCGACAGAGCCTTTACAGACCATGATTTAATGAACAAAATCACAAAAAACGGCCGGca              <  2:3439345/140‑1 (MQ=255)
                                                                                                                            ccacaCGCAGAGGTTGTAAACCCCGACGACAACGGGCTTCTCATAGACAGCGACAACAACCCCGCTTCATAGGCCAAACCAATCGACACAGCCTTTACAGAACAAGAATTAATGAACAAAATCACAAAAAACGGCCGGca              <  2:4055611/136‑1 (MQ=255)
                                                                                                                              aaaCGGAGAGGTTTTAAAACACGAAGTAAACGGCCTTTTAATCGACAGCTAAAACAAACCCTCTTAATTTGCTAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACAAAAAACGGCCGGcaca            <  2:1033369/140‑1 (MQ=255)
                                                                                                                                 cGGAGAGTTTGTAAAACACGAAGTAAACGGCCTTGTCATCGACAGATACAAAAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACAAAAAACGGCCGGCACAATg         <  2:3837481/140‑1 (MQ=255)
                                                                                                                                 cGGAGAGGTTGAAAAACACGAAGTCAACGCCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACCGACCAAGAATTAATGAACAAAATCACAAAAAACGGCCGGCACAATg         <  2:3140720/140‑1 (MQ=255)
                                                                                                                                        gTTTTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGAc                                                                   <  1:2725241/75‑1 (MQ=255)
                                                                                                                                        gTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGAc                                                                   >  2:2725241/1‑75 (MQ=255)
                                                                                                                                        gTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACAAAAAACGGCCGGCACAATGTAGAATc  >  1:4054299/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CATTCCAAATCTCTTTCTGATGGCAGATGTATTTGTGTGCAGCTCTCAGTGGAATGAACCCCTCGCCCGCGTGAATTATGAAGCAATGGCGGCCGGAACGCCCTTAATTACCACAAACCGGGGCGGAAACGGAGAGGTTGTAAAACACGAAGTCAACGGCCTTGTCATCGACAGCTACAACAAACCCTCTTCATTTGCGAAAGCAATCGACAGAGCCTTTACAGACCAAGAATTAATGAACAAAATCACAAAAAACGGCCGGCACAATGTAGAATC  >  NZ_CP034943/2962494‑2962769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: