Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,979,248 T→C 31.2% noncoding (1553/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,979,2480TC31.2% ‑1.9 / 34.4 45noncoding (1553/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (6/24);  new base C (2/12);  total (8/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ATCACCTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCA  >  NZ_CP034943/2979109‑2979383
                                                                                                                                           |                                                                                                                                       
ataaCCTTAGGATTCTATCCTCGCCTACCTGTGTAGGTTTGCGGTACGCGCACCTCTCACCTCGCTAGAGGCTTTTCTTCGCAGTGTCCAATCAGGAACTTCGCTACTAAATTTAGCTCGCCATCACAGCTCAGCCTTAt                                                                                                                                         >  2:349292/4‑140 (MQ=1)
     ctgaGGATTCTCTACTCGCCTACCTGTGTCGGTTTGCGGTTCGGGCACCTCTCTCCTCGCGAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACACCTCAGCCTTCTGGGaa                                                                                                                                    <  2:3685390/137‑1 (MQ=1)
      ttcggAGTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCGACTACAGGTCGCTCGCCATCACAGCTCAGCCTTACGGGaaa                                                                                                                                   <  2:607093/137‑1 (MQ=1)
          gagtctctcCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCCTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTGACGGGAAACGGa                                                                                                                               <  2:4959704/137‑1 (MQ=0)
          gagtctctcCTCGCCTACCTGGGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGa                                                                                                                               <  2:4014151/137‑1 (MQ=1)
               tatactagCCTACCTGTGTCTGTTTTAGATACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGCATTTGc                                                                                                                          >  2:1351734/8‑140 (MQ=1)
                       ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCTCCTAGCTAGAGGCTTTTCTTGGCAGTGTGGAATCATGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGTAAACGGATTTGCCTATTTcc                                                                                                                  <  2:2073830/137‑1 (MQ=1)
                       ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  1:4543255/137‑1 (MQ=1)
                       ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTGTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  2:2793182/137‑1 (MQ=1)
                       ccgaCCTGTGTCGGTTTGCGGTACGGGCACCTCTCAACTCGCGAGAGGCTTTTCTTGGCAGTGTGGCATCAGGCACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTcc                                                                                                                  <  2:2819643/137‑1 (MQ=1)
                           ccggtgtCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGc                                                                                                              >  2:4624560/4‑140 (MQ=1)
                                        gctgttcGGGCAGCTCTGACCTCGCTTGAGGCTTTTCTTGGCAGTGTGGAATGAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGa                                                                                                 <  2:2395053/134‑1 (MQ=1)
                                             accggCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCgg                                                                                            <  1:982421/137‑1 (MQ=1)
                                             aaggtcACCTCTCACCTCGCTAGAGGCTTTTCTTTGCAGTGTGGAATCAGGTACTTCTCTACTAAATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACTTATTTGACTATTTCCCAGCCTAACTGCTTGGACGCgg                                                                                            <  2:1729079/135‑1 (MQ=1)
                                              cattcaactctCACCTCGCTAGAGTATTATATTCACAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCAATTTCCCAGCCTAACTGCTTGGACGCGGa                                                                                           >  1:2414358/8‑140 (MQ=1)
                                                        tctccTCGCGAGAGGCTCTTCGTGGCCGTGTGGCATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTACCTGCTTGGACGCGGATATCCAATAc                                                                                 <  2:4292198/137‑1 (MQ=1)
                                                        tccccTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACCTTTCGCCCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  1:5129672/137‑1 (MQ=0)
                                                        tccccTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  2:3533782/137‑1 (MQ=1)
                                                        tccccTCGCTAGAGGCATGTCGTGGCAGTGTGGAATCAGGAACTTCGCGACTACATTTCGCGCGCCATCACAGCTCAGCCTTACGGGCACCGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  2:3613872/137‑1 (MQ=1)
                                                        cccccgcGCTAGAGGCTTTTCTTGGCAGTGGGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATAc                                                                                 <  2:2831882/134‑1 (MQ=0)
                                                        accccccGCCAGAGGCGTTACTTGGCCGCGTGCAATCAGGCACTTCGCTACTACCTGTCGCTCGCCATCACAGCTCAGCCTTACGGCACACGGATTTGCCTAGTTCCCAGCCTAACAGCTTGGACGCGGATATCCAATAc                                                                                 <  2:2233256/134‑1 (MQ=1)
                                                            ctagCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACcgcg                                                                             <  2:3376451/137‑1 (MQ=1)
                                                             tctcTAGAGGCTTTTCTTGGCAGTGTGGAATCAGTAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCgcgc                                                                            <  2:3158244/137‑1 (MQ=1)
                                                             tctcTAGAGGCTTGTCTTGGCAGTGTGGAATCAGTAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAAGTGCTTGGACGCTGATATCCAATACCgcgc                                                                            <  2:245715/137‑1 (MQ=1)
                                                               gcgagagGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCtt                                                                          >  1:890102/4‑140 (MQ=1)
                                                               gcgagagGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCtt                                                                          >  2:479598/4‑140 (MQ=1)
                                                                  tgatgCTTTTCTTGGGAGTGTGGAATGAGGAACTTCGCTACTACATTTCTCTCGCAATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTAcc                                                                       <  2:4267460/136‑1 (MQ=1)
                                                                     gggttttCTTGGCAGTGTGGAATCAGGAACTTCGCTACTCCATTTCTCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCGTATTTGCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTa                                                                    <  2:5181171/137‑1 (MQ=1)
                                                                      gcgtttCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTAt                                                                   <  2:4155717/137‑1 (MQ=1)
                                                                             tttgCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCATCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTg                                                            <  1:3620098/137‑1 (MQ=1)
                                                                                    tttgcaaTAAGGAACTTCGCTACTCCAATACGATCGCCATAACAGATCAGCCTTATGGTAAACGGATTTGCCTATTTCACAGCATAACTGGTTGGACGCGGATATCCAATACAGCGCGTACCCTATCCTCCTGCGTcccc                                                     >  2:4573745/6‑140 (MQ=1)
                                                                                    tgaggAAGCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCGTATGGGAAACGGATTTGCCTATTTCCCCGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTcccc                                                     >  2:1973865/4‑140 (MQ=0)
                                                                                              ggcaCTTCGCTACTACATTTCGCTCGCCATCCCAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCACCCCAGTGCTCa                                           >  1:3395310/4‑140 (MQ=0)
                                                                                                 acgtCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTACGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAAGACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAAt                                        <  1:2165623/137‑1 (MQ=0)
                                                                                                      gcaactacAGTTCGCTCGCCAGCCCCGCGCCGCCGGAGGGGAAACGGAGGGGCCTAGGTCCCAGCCGAACTGCTGGCACGCGGAGATCCAATCCCGCCCGGACCCTATCCTCCTGCGTCCCCCCAGTGCTCACATGGTgc                                   <  2:2925654/137‑2 (MQ=0)
                                                                                                           tcccttgcgcgcgcCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGaggagg                              <  2:3291403/133‑1 (MQ=0)
                                                                                                           tataTTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGaggagg                              <  1:2604962/137‑1 (MQ=1)
                                                                                                           tagaTTTCGCTCGCCATCACAGCTCAGCCTTATGTGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCTGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCACCATTGCTCAAATGGTGaggagg                              <  1:3537641/137‑1 (MQ=0)
                                                                                                            acctttCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAACTGGTGAGGAggt                             <  1:2807405/137‑1 (MQ=1)
                                                                                                                   gcgcgcCCTCACAGCTCAGCCCTCTGGGACACGGATGTGCCGAGTTCCCCGCCGACCGGCTTGCACGCGGATATCCACTACCGCGCTTACCCTATCCGCCTCCGTCCCCCCAGTGCTCCAAGGGTGAGGAGGTGGTACAg                      <  2:3572484/137‑1 (MQ=0)
                                                                                                                   gcgcgcCATCACAGCTCACCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTCACTGCGTGGACGCGGATATCCAATACCGCGCGTACCCTATCCTCCCGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAg                      <  2:4161553/137‑1 (MQ=1)
                                                                                                                    ctagcCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCAGTGCTCAAATGGTGAGGAGGTGGTACAgg                     <  2:1602879/137‑1 (MQ=1)
                                                                                                                                gcccAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCtg         <  1:4222424/137‑1 (MQ=1)
                                                                                                                                 ctaaGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCtgt        <  2:1179818/137‑1 (MQ=1)
                                                                                                                                      ccgtATGGGAAACGGATTTGCCTATGTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTcc   <  1:2898/137‑1 (MQ=1)
                                                                                                                                       ctgaTGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGAGATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCa  <  1:1847723/137‑1 (MQ=1)
                                                                                                                                           |                                                                                                                                       
ATCACCTTAGGATTCTCTCCTCGCCTACCTGTGTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGCAGTGTGGAATCAGGAACTTCGCTACTACATTTCGCTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAGCCTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTGTTGTCCA  >  NZ_CP034943/2979109‑2979383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑