BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	132,099	T→C	62.4%	V531A (GTA→GCA)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,099	0	T	C	62.4%	346.2 / inf	372	V531A (GTA→GCA)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base T (56/84); new base C (72/160); total (128/244)
Fisher's exact test for biased strand distribution p-value = 9.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 372 total aligned reads displayed.

TCCGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTG  >  NZ_CP034943/131961‑132227

tCCGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTa                                                                                                                                 >  1:3132210/1‑140 (MQ=255)

  cGTACCGGTGCAACAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTGGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGt                                                                                                                               <  2:3153590/140‑1 (MQ=255)

  cGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGt                                                                                                                               <  2:1671070/140‑1 (MQ=255)

  cGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGt                                                                                                                               <  2:2475478/140‑1 (MQ=255)

    tACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTc                                                                                                                             <  1:2683998/140‑1 (MQ=255)

    tACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTc                                                                                                                             <  1:1980550/140‑1 (MQ=255)

         gTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGt                                                                                                                        >  2:2895137/1‑140 (MQ=255)

         gTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGt                                                                                                                        >  1:3894432/1‑140 (MQ=255)

          tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                       >  2:1956622/1‑140 (MQ=255)

          tGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTg                                                                                                                       >  1:3579092/1‑140 (MQ=255)

            cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGaa                                                                                                                     <  1:2895137/140‑1 (MQ=255)

            cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGaa                                                                                                                     <  2:706154/140‑1 (MQ=255)

            cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                     >  2:3199264/1‑140 (MQ=255)

            cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                     <  1:598759/140‑1 (MQ=255)

            cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                     >  1:808539/1‑140 (MQ=255)

            cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                     <  1:3258661/140‑1 (MQ=255)

            cAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTGTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                     >  1:2388598/1‑140 (MQ=255)

            cAAAACTTGAAGGTACATTCGGACGTCAGTCTGGGGGACGCGGTCCGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGaa                                                                                                                     <  2:265024/140‑1 (MQ=255)

                    gAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATccc                                                                                                             >  1:1732184/1‑140 (MQ=255)

                      aGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGACGGCGCGTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCTAg                                                                                                           <  2:2532271/140‑1 (MQ=255)

                      aGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAg                                                                                                           <  2:2523739/140‑1 (MQ=255)

                      aGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAg                                                                                                           <  2:386566/140‑1 (MQ=255)

                      aGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAg                                                                                                           <  1:1002065/140‑1 (MQ=255)

                      aGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAg                                                                                                           <  1:3110410/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:1191364/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:4210279/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:1381888/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:4537954/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:2035598/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:4884796/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:4078534/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:402666/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:4423456/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:1455100/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:4930579/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:1290823/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:1555489/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:4656815/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:1264662/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:1868034/140‑1 (MQ=255)

                               cGTACGTCAGTCTGGTGGACGCGGGCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:4046623/140‑1 (MQ=255)

                               cGTACGTCAGTCGGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  2:3960209/140‑1 (MQ=255)

                                 tACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCa                                                                                                >  2:815881/1‑140 (MQ=255)

                                    gTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGt                                                                                             <  2:658932/140‑1 (MQ=255)

                                       aGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTg                                                                                          >  1:2762505/1‑140 (MQ=255)

                                       aGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTg                                                                                          >  2:1796647/1‑140 (MQ=255)

                                        gTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTgg                                                                                         >  1:5134189/1‑140 (MQ=255)

                                        gTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTgg                                                                                         >  1:3428446/1‑140 (MQ=255)

                                         tCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGa                                                                                        <  1:2489395/140‑1 (MQ=255)

                                         tCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGa                                                                                        <  1:4481161/140‑1 (MQ=255)

                                              tggACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATg                                                                                   <  1:4103405/140‑1 (MQ=255)

                                              tggACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATg                                                                                   <  2:1420057/140‑1 (MQ=255)

                                                gACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCa                                                                                 <  1:2332412/140‑1 (MQ=255)

                                                gACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCa                                                                                 <  1:1857293/140‑1 (MQ=255)

                                                  cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGACGGCGCTTGCTTTGAATTTGAAAATGCGAGTGTCGGTGGGGCAGTTCCACGGGTATACATCCCAGCTGTTCAAGCCGGTCTGGAAGATGCCCt                                                                               >  1:907095/1‑140 (MQ=255)

                                                  cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAg                                                                                                  <  1:3851490/121‑1 (MQ=255)

                                                  cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGACGATGCACt                                                                               >  2:4391019/1‑140 (MQ=255)

                                                  cgcgGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACt                                                                               >  1:2281222/1‑140 (MQ=255)

                                                        cAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGaaaa                                                                         <  2:1465417/140‑1 (MQ=255)

                                                        cAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGaaaa                                                                         <  2:559714/140‑1 (MQ=255)

                                                         aGTTCGGACACGTTTGGATCGAATTCGAACCACACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGAGTGTCGGTGGGGCAGTTCCACGTGAATACAGCCCAGCAGGTCACGCAGGTCTGGAAGATGCACTTGAAAAt                                                                        <  1:3804250/140‑1 (MQ=255)

                                                            tCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGgt                                                                     <  2:5137491/140‑1 (MQ=255)

                                                                     tttGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCa                                                            >  1:1110764/1‑140 (MQ=255)

                                                                          ctccaaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACCTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCtt                                                       <  1:106910/136‑1 (MQ=255)

                                                                            cGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTcc                                                     <  2:1732184/140‑1 (MQ=255)

                                                                            cGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTcc                                                     <  2:4150327/140‑1 (MQ=255)

                                                                              aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCg                                                   >  2:3708820/1‑140 (MQ=255)

                                                                              aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCg                                                   >  1:5206607/1‑140 (MQ=255)

                                                                              aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCg                                                   >  1:1784479/1‑140 (MQ=255)

                                                                              aaTTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCg                                                   >  2:3565140/1‑140 (MQ=255)

                                                                                   gAACCAAACTAAGAAGGCGCTTGCTGTGAATTTGAACATGCGATTGTCGGTGGGGTCGTTCCACGTGACTACATCCCAGCTCGCCAAGCAGGTCTGCAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTAt                                              <  2:349975/140‑1 (MQ=255)

                                                                                   gAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTAt                                              <  2:2761512/140‑1 (MQ=255)

                                                                                    aaCCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATc                                             <  2:1020520/140‑1 (MQ=255)

                                                                                         aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACAt                                        >  1:2117599/1‑140 (MQ=255)

                                                                                         aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTGATcatcat                                        >  1:4738776/1‑135 (MQ=255)

                                                                                         aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                        <  1:1670189/140‑1 (MQ=255)

                                                                                          aCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAAt                                                                        >  2:2101524/1‑107 (MQ=255)

                                                                                               gaagGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGc                                  >  1:4979552/1‑140 (MQ=255)

                                                                                                   gcgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCaaaa                              >  1:1566089/1‑140 (MQ=255)

                                                                                                    cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAAc                             >  2:3384094/1‑140 (MQ=255)

                                                                                                    cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATc                                             >  1:4236007/1‑124 (MQ=255)

                                                                                                    cgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATc                                             >  1:369064/1‑124 (MQ=255)

                                                                                                          cttTGAATTTGAAACTGCGATTGTCTGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGTAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCg                       <  1:747898/140‑1 (MQ=255)

                                                                                                          cttTGAATTTGAAAATGCGATTGTCGGTTGGGTATTTCCACGTGAATACATCCCAGCTTTTGAAGCAGGTATGTACGATGCACTTGAAAATGGTTGACTGGCAGGCTTCCCGCTTATCGACATTTAAGCAAAAGTTTTCg                       <  2:4265399/140‑1 (MQ=255)

                                                                                                           tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                      <  1:5049454/140‑1 (MQ=255)

                                                                                                           tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                      <  2:4960139/140‑1 (MQ=255)

                                                                                                           tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                      <  2:1080416/140‑1 (MQ=255)

                                                                                                           tttgaatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                      <  1:3701239/140‑1 (MQ=255)

                                                                                                               aatttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGAAAAACTTTTCGATGGt                  >  2:3928605/1‑140 (MQ=255)

                                                                                                                atttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGtt                 <  2:4829927/140‑1 (MQ=255)

                                                                                                                atttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGtt                 <  2:2494217/140‑1 (MQ=255)

                                                                                                                atttgaaAATGCGATGGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGtt                 <  2:3265738/140‑1 (MQ=255)

                                                                                                                 tttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTc                <  1:1894869/140‑1 (MQ=255)

                                                                                                                 attgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACATGAAAATTGGGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTc                <  1:4779796/139‑1 (MQ=255)

                                                                                                                  ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCt               <  2:224318/140‑1 (MQ=255)

                                                                                                                  ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCt               <  2:892948/140‑1 (MQ=255)

                                                                                                                  ttgaaAATGCGATTGTCGGTGGGGCAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCt               <  1:635175/140‑1 (MQ=255)

                                                                                                                    gaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTa             >  1:1541650/1‑140 (MQ=255)

                                                                                                                               tGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTg  >  2:3619615/1‑140 (MQ=255)

                                                                                                                               tGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTg  >  1:4416413/1‑140 (MQ=255)

TCCGTACCGGTGCAAAAGTTGAAGGTAAATTCGTACGTCAGTCTGGTGGACGCGGTCAGTTCGGACACGTTTGGATCGAATTCGAACCAAACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTG  >  NZ_CP034943/131961‑132227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`