Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,268,725 C→A 9.4% intergenic (+73/+137) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,268,7250CA 9.4% 721.8 / 18.9 282intergenic (+73/+137)catR/EO946_RS16920catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (40/205);  new base A (35/2);  total (75/207)
Fisher's exact test for biased strand distribution p-value = 2.26e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

GGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACAT  >  NZ_CP034943/3268586‑3268856
                                                                                                                                             |                                                                                                                                   
ggCCCGCATCTTGGGTGATATTTCTAAATGGGCGACGGATTTGATTGACCCTTCGTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAATGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAc                                                                                                                                     <  2:3478240/140‑1 (MQ=255)
ggCCCCCATCTTGGTTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAc                                                                                                                                     <  1:851602/140‑1 (MQ=255)
   ccccATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGAc                                                                                                                                  >  2:325088/1‑140 (MQ=255)
     ccATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTTTTAATTTTGCAGGTAAAAAAAGACtt                                                                                                                                >  1:3993086/1‑140 (MQ=255)
      cATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtc                                                                                                                               >  2:1689239/1‑140 (MQ=255)
        tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                             <  1:1837811/140‑1 (MQ=255)
        tCTTGGGTGAGATTTCTAAAGGGGCGACGGATTGGATTGACCCTTACTTTCTTTACTAATTGCTAAACCGGTTCTGATCTAAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                             <  1:2139847/140‑1 (MQ=255)
           tcggagagaTTTCTCACTGGGCGACGCATTCGATTGACCCTTCATTTATTGAGTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGACAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:3075055/135‑1 (MQ=255)
           tGGGTGATATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:63384/140‑1 (MQ=255)
           tGGGTGATATTTCTAAATGGGCGACGGATTGGATTGACCATTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGAGAGAAAAGATCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACCTCTCAGc                                                                                                                          <  1:4766795/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTTATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:3684860/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTGCGAGGGAAAAAACGACTtctaatc                                                                                                                          >  2:4720545/1‑136 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:1260947/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:4071367/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:3953931/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:4875017/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:3292900/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:4457536/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:252072/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  2:2343550/140‑1 (MQ=255)
           tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGCGGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                          <  1:1475253/140‑1 (MQ=255)
           ggggTGATATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTAATTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGTTAAAAAACGACTTCTCAGc                                                                                                                          <  1:141847/139‑1 (MQ=255)
           cgcgTGAGATTTCTAACTGGGCGACGGATTGGATTGACCCTTCCGTTCTTCACTAATTCCTAAAACGGTTCTGATCTAAAAGGCGAGAAAAGAGCCGTTTTTTTATTTGTCATTTGCCAGGTCAAAAACGACTTCTCAGc                                                                                                                          <  2:1480930/137‑1 (MQ=255)
            gggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTTTTTTTGATTTGCCCTGTAAAAAACGACTTGTGAGcc                                                                                                                         >  2:4117554/1‑140 (MQ=255)
            gggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGcc                                                                                                                         <  1:3050677/140‑1 (MQ=255)
            gggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCGGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAAAGACttataagac                                                                                                                         >  2:206968/1‑133 (MQ=255)
             ggTGATATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACGAATTGCTAACACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                        <  2:772517/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTTACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                        <  2:1396594/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTTATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                        <  2:883716/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGTAAAAAAAGACTTCTCAGCCg                                                                                                                        >  2:2392088/1‑140 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                        <  1:4233134/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                        <  2:4069916/140‑1 (MQ=255)
             ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACGTCTCAGCCg                                                                                                                        <  2:1763358/140‑1 (MQ=255)
              gTGAGATTTATAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGa                                                                                                                       <  1:3523568/140‑1 (MQ=255)
               tGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGat                                                                                                                      <  1:1039226/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTTACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                     <  1:1370037/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                     <  2:2470921/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                     <  1:1764462/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                     <  2:2194797/140‑1 (MQ=255)
                gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                                     <  1:958027/140‑1 (MQ=255)
                 agaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTTGCAGGTAAAAAACGACTTCTCAGCCGatat                                                                                                                    >  2:352915/1‑140 (MQ=255)
                 agaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCATGTAAAAAACGACTTCTAAGCCGatat                                                                                                                    >  2:3825918/1‑140 (MQ=255)
                 agaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGTAAAAAAAGCCTTATCAGCCGctat                                                                                                                    >  1:4061780/1‑140 (MQ=255)
                 agaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatat                                                                                                                    >  2:3274110/1‑140 (MQ=255)
                 agaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTAATTTTCATTTTCGAGGGAAAAAAAGACATCTCcgacgaaag                                                                                                                    >  1:222435/1‑137 (MQ=255)
                  taTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCGTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                   <  2:3374625/139‑1 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                   <  1:4921357/140‑1 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                   <  2:2842513/140‑1 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                   <  2:2791233/140‑1 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                   <  1:448121/140‑1 (MQ=255)
                  gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                                   <  1:2810472/140‑1 (MQ=255)
                   aTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTAATTTTAATTTTCCAAGTAAAAAAAGACTTCTAAGCCGctatat                                                                                                                  >  1:1124405/1‑140 (MQ=255)
                     ttCTAAATGGGCGACTGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtag                                                                                                                <  2:3244930/140‑1 (MQ=255)
                       cTAAATGGGCGACGTATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtagta                                                                                                              <  2:3993188/140‑1 (MQ=255)
                          aaTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGaa                                                                                                           <  1:3255854/140‑1 (MQ=255)
                           aTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAAc                                                                                                          <  1:2493383/140‑1 (MQ=255)
                            tGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAAcc                                                                                                         <  1:3512515/140‑1 (MQ=255)
                                          gattgACCCTTCCTTTCTTGACTAATTGCTAAAATGGTTCTGATCTCAAAGACGAGAAAAGAACCGTTTTTTATTTT‑‑‑‑‑‑‑CCAGGTGAAAAAAAGATATCTCAGCCGATATATAGTACATCCACACATTACGTTTTTaaagga                                                                                    <  2:5175173/140‑6 (MQ=255)
                                               aCCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGGAAAAAACGACTTCAAAGCCGATATATAGTAGAACCCCACCTTGCGGTTTTAGcc                                                                                      >  2:580250/1‑140 (MQ=255)
                                                cccTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTTATTTTGCGGGTAAAAAAATAATTCTCAGCCGATATATATTTTAAACCCACAATTGGttttttgagg                                                                                     >  1:2668741/1‑135 (MQ=255)
                                                cccTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCg                                                                                     <  1:4540079/140‑1 (MQ=255)
                                                cccTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCACAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGTTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCg                                                                                     <  1:4831933/140‑1 (MQ=255)
                                                cccGTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCg                                                                                     <  1:292385/140‑1 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTGTTTATCCgaaga                                                                                 >  2:2754034/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTAGCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCgaaga                                                                                 >  2:1196505/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTTCCAGGTAAAAAACGACTTATCATCGGATATATAGTATAAACCCACATTTCGTTTTTAGCCgaaga                                                                                 >  2:4417359/1‑140 (MQ=255)
                                                    tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGGTTTTATTTTTAATTTGCCCGGTAAAAAACGAATTCTCAGCCGAGATATATTAGAACCCCACCTTGCGTTTTTTGGagaaga                                                                                 >  1:4434099/1‑140 (MQ=255)
                                                     ccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAAAGACTTCACCGCCGATATATAGTAGAAACCCACATTTCGTTTTTAGCCgaacaa                                                                                >  2:2900580/1‑140 (MQ=255)
                                                     ccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTCATTTTCCCGGTAAAAAACGACTTCTAAGCCGATAAATAGTAGAACCCCCCATTGCGTTTTTATCCgaagaa                                                                                >  2:2227086/1‑140 (MQ=255)
                                                             gACTAATTTCTAAAACGGTACTGATCTCACAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATAGATAGTAGAACCCCACAGGGCGTTTTTAGCCGAAGAATTTGagca                                                                        <  2:4162979/140‑1 (MQ=255)
                                                                   ttGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCCGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAAACCCCCATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGc                                                                  >  2:186700/1‑140 (MQ=255)
                                                                             gggtCTGACCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                        <  2:1803076/137‑1 (MQ=255)
                                                                              gTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTATAACCCCACATTGCGTTTTTTGCCGAAGAATTTGAGCAGCTGGCGTAAGAGCTTc                                                       >  2:1137696/1‑140 (MQ=255)
                                                                               tgcTGATCTCAAACGCGAGAAAAGAGCCGTTTTTTTATTTGTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATAGATTGTAGAACCCCACACTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAACGAGCTTCa                                                      <  2:1976952/138‑1 (MQ=255)
                                                                                tCTTATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                     <  2:5144064/140‑1 (MQ=255)
                                                                                tCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCAt                                                     <  1:428785/140‑1 (MQ=255)
                                                                                tCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTATTTTTGATTTTCCCGGTAAAAAAACACTTCTCAGCCGATATTTAGTAGAACCCCACCTTGCGTTTTTAGGCGAACAATTTTTGCAGCTGGCGCAAGAGCTTCAt                                                     >  1:3630635/1‑140 (MQ=255)
                                                                                  tGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATga                                                   <  1:466252/140‑1 (MQ=255)
                                                                                  tGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATga                                                   <  1:2508184/140‑1 (MQ=255)
                                                                                      ctcAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaag                                               <  1:5208888/140‑1 (MQ=255)
                                                                                       tcAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                              <  1:1786956/140‑1 (MQ=255)
                                                                                         aaaGGCGAGAAAAGAGCCGTTTTTTTATTTTTCAATTTGCCGGTAAAAAACGACTTGTGAGCCGATATATAGTAGAAACCCACAATGCGTTTTTAGGCGAAGAATATGGGCCGCTGGCGAAAGAGGGTCCTGAGCAGATg                                            >  1:5080143/1‑140 (MQ=255)
                                                                                         aaaGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATg                                            >  2:1064840/1‑140 (MQ=255)
                                                                                                 taaaaGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGAATTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                    <  1:2480397/139‑1 (MQ=255)
                                                                                                 gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                    <  1:2574333/140‑1 (MQ=255)
                                                                                                 caaaaGAGCCGTTTTTTTATTTTTCATTTGCCCGGTAAAAAACGACTTCTCAGCCGATAGATAGTACAACCCCACATTTCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                                    <  1:236834/139‑1 (MQ=255)
                                                                                                  aaaaGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAATCGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGATGATGGATCAGTTc                                   <  2:4257182/140‑1 (MQ=255)
                                                                                                       agCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAAATTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACAtt                              <  2:359407/140‑1 (MQ=255)
                                                                                                         ccGTTTTTTTATTTTTCATTTTCCAGGTAAAAAACGAATTCTCAGCCGATATATATTAGAACCCCACATTTCGTTTTTATCCGAAGAATTTTAGCAGCTGGCGAAAGAGCTTAATGAGAAGATTGATAAGTTCAAATTTa                            >  2:1443393/1‑140 (MQ=255)
                                                                                                                 ttATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCCCCTTGGGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATTTATCAGTTCACATTTAAAATAAcc                    <  1:739193/140‑1 (MQ=255)
                                                                                                                 ttATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc                    <  1:3347053/140‑1 (MQ=255)
                                                                                                                 ttATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc                    <  1:3576221/140‑1 (MQ=255)
                                                                                                                 ttATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc                    <  1:1641967/140‑1 (MQ=255)
                                                                                                                 ttATTTTTCATTTGCCAGGAAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc                    <  1:4117554/140‑1 (MQ=255)
                                                                                                                 ttATTTTTAATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc                    <  1:590283/140‑1 (MQ=255)
                                                                                                                   aTTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCa                  <  2:262602/140‑1 (MQ=255)
                                                                                                                   aTTTTTCATTTGCAATGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCa                  <  2:2974400/140‑1 (MQ=255)
                                                                                                                       ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc              <  2:1907098/140‑1 (MQ=255)
                                                                                                                            tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa          <  1:3768767/140‑1 (MQ=255)
                                                                                                                            tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa          <  1:1226622/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                   
GGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGATAGGACAT  >  NZ_CP034943/3268586‑3268856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: