Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,274,191 G→T 6.9% intergenic (‑528/+58) EO946_RS16945 ← / ← EO946_RS16950 NisI/SpaI family lantibiotic immunity lipoprotein/gallidermin/nisin family lantibiotic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,274,1910GT 6.9% 467.5 / 2.5 189intergenic (‑528/+58)EO946_RS16945/EO946_RS16950NisI/SpaI family lantibiotic immunity lipoprotein/gallidermin/nisin family lantibiotic
Reads supporting (aligned to +/- strand):  ref base G (44/121);  new base T (13/5);  total (62/127)
Fisher's exact test for biased strand distribution p-value = 1.96e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

CATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATCCTGGTGTACA  >  NZ_CP034943/3274053‑3274329
                                                                                                                                          |                                                                                                                                           
cctGATCGAACCTAACCGTTCTTCCGTTCTACAATGGTGTTCCTCCTGAACCCCGAACATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTACGAAAATAAgt                                                                                                                                            <  2:5118693/138‑1 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgt                                                                                                                                            <  1:1711476/140‑1 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgt                                                                                                                                            <  1:2442119/140‑1 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgt                                                                                                                                            <  2:4170647/140‑1 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgt                                                                                                                                            <  2:2282774/140‑1 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgt                                                                                                                                            <  1:4540952/140‑1 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTGTTTTACTTGATTTTATTGTGCCGACTCTATGAAAATAAgt                                                                                                                                            >  1:1606955/1‑140 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTCCACACCCGTCGTTTTTTAATTAATTTTCTTTTGCCCACTCTATGTAAAAAAgt                                                                                                                                            >  1:2733867/1‑140 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACCCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgt                                                                                                                                            <  2:1871460/140‑1 (MQ=255)
cATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGGTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgt                                                                                                                                            <  1:2307974/140‑1 (MQ=255)
  tGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTCTCCCGACTCTATGAAAATAAgtat                                                                                                                                          <  2:4928938/140‑1 (MQ=255)
     tCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTCAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgtatgta                                                                                                                                       <  1:2874709/140‑1 (MQ=255)
     tCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgtatgta                                                                                                                                       <  1:1400392/140‑1 (MQ=255)
     tCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAgtatgta                                                                                                                                       <  2:2570806/140‑1 (MQ=255)
       gAAAATAACCGTTCTTCCGTTCTACACTTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGtata                                                                                                                                     <  1:575552/140‑1 (MQ=255)
       gAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGtata                                                                                                                                     <  2:1589543/140‑1 (MQ=255)
       gAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGtata                                                                                                                                     <  1:818812/140‑1 (MQ=255)
         aaaTAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTTATTAAGTTTCTTTTACCGACTGTATTAAAATAAGTATGTATaaa                                                                                                                                   >  1:3163153/1‑139 (MQ=255)
         aaaTAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTGGTTTTAATAATTTTCTTGTGCCGACACTATTAAAAAAAGTATGTTTAac                                                                                                                                   >  1:907211/1‑140 (MQ=255)
          aaTAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAaca                                                                                                                                  <  1:1192502/140‑1 (MQ=255)
          aaTAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAaca                                                                                                                                  <  2:1989914/140‑1 (MQ=255)
               ccGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCCTTTTCTTGTCCCGACTCTATGAAAATACGGATGTATAACACTCAg                                                                                                                             >  2:4059562/1‑140 (MQ=255)
               ccGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAg                                                                                                                             <  1:1629027/140‑1 (MQ=255)
               ccGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAg                                                                                                                             <  1:3454885/140‑1 (MQ=255)
               ccGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCGATGAAAATAAGTATGTATAACACTAAg                                                                                                                             >  2:3918492/1‑140 (MQ=255)
               ccGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTAATTTTCTTTTCCCGAATCTATGAAAATAAGTATGTATAAAACTCAg                                                                                                                             >  2:1945441/1‑140 (MQ=255)
                  gtcttcCGTTCTACACTTGTGTTCCTCCGTACAGCCTAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAg                                                                                                                         <  1:3513222/139‑1 (MQ=255)
                   tcttcCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTTATTAATTTTGTTGTGCCGACTCTATGAAAAAAAGTATGTATAAAACTAAGAAGt                                                                                                                        >  1:4195623/1‑140 (MQ=255)
                   tcttcCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTGTTTTTCTTCATTTTGTTGTCCCGACTCTCTGTAAATAAGTATTTATAACAAGCAGaatt                                                                                                                         >  1:5193558/1‑138 (MQ=255)
                      tcCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCa                                                                                                                     <  1:1896971/140‑1 (MQ=255)
                      tcCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCa                                                                                                                     <  1:1589741/140‑1 (MQ=255)
                      tcCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCATTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGTTAATATTTATTTATAACACTCAGAATTGct                                                                                                                     <  2:1371647/140‑2 (MQ=255)
                       ccGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCaa                                                                                                                    <  1:193430/140‑1 (MQ=255)
                       ccGTTCTACAATGGTGTTCCTCCATAACGCCCAATATAAAAACGGCTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCaa                                                                                                                    <  1:3456686/140‑1 (MQ=255)
                           tCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAg                                                                                                                <  2:4296299/140‑1 (MQ=255)
                            cTACATTTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  1:4248247/140‑1 (MQ=255)
                            cTACAATTGTGTTCCTCCTTAAAGCCTAATATAAAAACGGGTGTTAATATAAACTCTTTCACACACGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  2:122720/140‑1 (MQ=255)
                            cTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  1:4871248/140‑1 (MQ=255)
                            cTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  2:1770328/140‑1 (MQ=255)
                            cTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  1:4418729/140‑1 (MQ=255)
                            cTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  1:1456768/140‑1 (MQ=255)
                            cTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  2:3700530/140‑1 (MQ=255)
                            cTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGt                                                                                                               <  1:2408468/140‑1 (MQ=255)
                                aaTGGTGGTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTGTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTCTAACACTCAGAAGTGCAAGGAGTCaga                                                                                                           <  2:5212014/140‑1 (MQ=255)
                                 aTTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCagag                                                                                                          <  1:4131530/140‑1 (MQ=255)
                                 aTTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCagag                                                                                                          <  1:1471644/140‑1 (MQ=255)
                                 aTTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCagag                                                                                                          <  1:599864/140‑1 (MQ=255)
                                    gtgtTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTAATTAATTTTATTGTCCCGACTCTATGAAAATAATTATGTATAAAACTAAGAAGTGGAAGGATTCAGAGCaa                                                                                                       >  1:3748375/1‑140 (MQ=255)
                                     tgtTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTGTTTTTCTTAATTTTATTGTCCCGACTATATTAAAATAAGTATTTATAAAAATAATAAGTGCAAGGAGTCAGATCAAg                                                                                                      >  2:4781006/1‑140 (MQ=255)
                                       ttCCTCCTTACAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGt                                                                                                    <  1:2974766/140‑1 (MQ=255)
                                       ttCCTCCTTAAAGCCGAATATAATAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTAATTGATTTTCTTTTCACGACTCGATGAAAATAAATATTTTTAACACTAAGAAGTGGAAGGAGTGAGAGCAAggg                                                                                                    >  1:720884/1‑139 (MQ=255)
                                       ttCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTGTTTTAATTAATTTTCTTGTCCCGACTCTAAGAAAAAAACTATGTATAAAAAAAAGAAATGCAAGGAGTCAGAGCAAGGt                                                                                                    >  1:1354949/1‑140 (MQ=255)
                                            cctTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAAAACACAAAAGTGCAAGGAGTCAAAGCAAGGTGATGc                                                                                               >  2:5100288/1‑140 (MQ=255)
                                               tAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTaa                                                                                            <  2:3493611/140‑1 (MQ=255)
                                               tAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTAATTGATTTTATTGTCCCCTCTCTAAGAAAATAAAAAATTATAAAACTAAAAAGGTGCAGGGGTCAGAGGACGGTGTTgggaa                                                                                            >  1:942608/1‑136 (MQ=255)
                                                 aaGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATg                                                                                          >  2:3416898/1‑140 (MQ=255)
                                                       aaTATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTTCTTGATTTTGTTTTGCCGACGCTTTGTAAATAATTATTTTTAAAACTCAGAAGTGGAAGGGGTGAGAGCAAGGTGTTGGTAATGGttttt                                                                                    >  1:3845923/1‑139 (MQ=255)
                                                       aaTATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAAAAAGTATGTATAAAAATCAGAAGTGGAAGGAGTCAGAGCAAGGTGATGCTAATGGGTTTa                                                                                    >  2:2214184/1‑140 (MQ=255)
                                                       aaTATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTACTTTTCTTGTCCCGACGCTATGAAAATAATTATGTATAAAAAACAGAAGTGCAAGGAGTCAGAGCAAGGTGATGGGAATGGTTTTa                                                                                    >  2:4617652/1‑140 (MQ=255)
                                                              aaaCGGGTGTGAATATAAACTCTTTCACACCCGTCGTGTTTTTCTTGATTTTCTTTTGCCGACGCTATGAAAATAAGTCTTTATAACAATAAGAAGTTGAAGGAGTCAGAGCCAGGGGGTGCGAATGGTTTTTGTTAttt                                                                             >  1:181307/1‑140 (MQ=255)
                                                              aaaCGGGTGTGAATATAAACTCTTTCACACCCGTCGTGTTTTAGTTGAGTTTGTTTTGCCGACTCTATTAAAATAATTATTTATAAAACACAGAAGATCAAGTAGTCAGAGGACGGTGTTGCTAATGGTTTTAGtttttt                                                                             >  1:4828899/1‑140 (MQ=255)
                                                              aaaCGGGTGTGAATATAAACTCTTTCACACCCGTCGTGTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATTTATAACAATCAGAATTGCAAGGAGTCAGAGCACGGTGATGCTAATGGTTTTACGTAgtt                                                                             >  1:3787174/1‑140 (MQ=255)
                                                                aCGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTag                                                                           <  2:3676084/140‑1 (MQ=255)
                                                                       tgAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGAtttt                                                                    <  1:5138100/140‑1 (MQ=255)
                                                                       tgAATATAAACTCTTTCACACCCGTAGTTTTTTACTTCATTTTCTTGTCCCGAATCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGAtttt                                                                    <  2:3821290/140‑1 (MQ=255)
                                                                        gAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTg                                                                   <  1:2106344/140‑1 (MQ=255)
                                                                                    tttCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGtt                                                       <  2:4520537/140‑1 (MQ=255)
                                                                                    tttCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGtt                                                       <  2:1354949/140‑1 (MQ=255)
                                                                                      tCACACCCGTCGTTTTTTACTTCATTTTGTTTTCCCGACTCTATGAAAATAAGTATTTATAACACTCAGAAGTGCAAGGAGTCAGAGGAAGGTGATGGTAATGGTTTTACTTATTTAGAGATTTTTGATTTAAAAGTTat                                                     >  2:728691/1‑139 (MQ=255)
                                                                                      tCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAg                                                     <  1:49931/140‑1 (MQ=255)
                                                                                            ccGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTg                                               <  1:3416898/140‑1 (MQ=255)
                                                                                                cgtTTTTTACTTCATTTTCTTTTACCGACTCTATGAAAATAAGTATGTATAACACTAACAAGTGCAAGGAGTCCGAGCAAGGTTATTCTAAAGGGTTTACGTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTtaagg                                           >  2:2006594/1‑140 (MQ=255)
                                                                                                cgtTTTTTACTTCATTTTATTGTCCCGCCTCGATGAAAATAAGTATGTATAACAAACAGAACTGCAACGAGTAAGACCAACGTGATGCTACTGGTTGTAATTATTTAAACATTTTTCAGTTAAAAGGTAGATTTTtaagg                                           >  2:3358473/1‑140 (MQ=255)
                                                                                                   tttttACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTgaaggaag                                        <  2:1102542/140‑1 (MQ=255)
                                                                                                   tttttACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTgaaggaag                                        <  2:2397534/140‑1 (MQ=255)
                                                                                                   tttttACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTgaaggaag                                        <  2:3203604/140‑1 (MQ=255)
                                                                                                   tttttACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTgaaggaag                                        <  2:328120/140‑1 (MQ=255)
                                                                                                   tttttAATTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTgaaggaag                                        <  2:5013908/140‑1 (MQ=255)
                                                                                                           tgaTTTTCTTGTCCCGACTCTCTGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGTTGCTAATGGTTGTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTg                                <  2:594718/138‑1 (MQ=255)
                                                                                                           tCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTg                                <  1:1111791/140‑1 (MQ=255)
                                                                                                            cATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGc                               >  2:2830233/1‑140 (MQ=255)
                                                                                                             aTTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCa                              <  1:2004418/140‑1 (MQ=255)
                                                                                                                 tcgtctCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGc                          <  2:1112163/135‑1 (MQ=255)
                                                                                                                 tattGTCCCTACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAATGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGc                          <  1:2176807/138‑1 (MQ=255)
                                                                                                                 tCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGc                          <  1:1052448/140‑1 (MQ=255)
                                                                                                                 tCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGc                          <  1:397459/140‑1 (MQ=255)
                                                                                                                          gACTCTATGAAAATAATTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTac                 <  2:4255029/140‑1 (MQ=255)
                                                                                                                          gACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGGAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTac                 <  2:1664621/140‑1 (MQ=255)
                                                                                                                          gACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCTGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTac                 <  2:2110782/140‑1 (MQ=255)
                                                                                                                          gACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTac                 <  1:170368/140‑1 (MQ=255)
                                                                                                                          gACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTac                 <  1:2588945/140‑1 (MQ=255)
                                                                                                                           aCTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCACGTTTGCAATGCACCAGTTaca                >  1:2647853/1‑140 (MQ=255)
                                                                                                                           aCTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTaca                <  1:4654587/140‑1 (MQ=255)
                                                                                                                               tctGAAAATAACTATGTATACCACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATc            <  2:3101542/138‑1 (MQ=255)
                                                                                                                                aTGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATcc           >  2:3565886/1‑140 (MQ=255)
                                                                                                                                 tGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATCCt          >  2:1070330/1‑140 (MQ=255)
                                                                                                                                  gAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATCCTg         <  1:4617652/140‑1 (MQ=255)
                                                                                                                                   aaaaTAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTGAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATCCTgg        <  1:1848306/140‑1 (MQ=255)
                                                                                                                                         aGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTTAAGGAAGCAAGTTTGCAATGCACCAGTTACACATCCTGGTGTACa  >  2:4604051/1‑140 (MQ=255)
                                                                                                                                         aGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATCCTGGTGTACa  >  2:840235/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CATGATCGAAAATAACCGTTCTTCCGTTCTACAATTGTGTTCCTCCTTAAAGCCGAATATAAAAACGGGTGTGAATATAAACTCTTTCACACCCGTCGTTTTTTACTTCATTTTCTTGTCCCGACTCTATGAAAATAAGTATGTATAACACTCAGAAGTGCAAGGAGTCAGAGCAAGGTGATGCTAATGGTTTTACTTATTTAGAGATTTTGCAGTTACAAGTTAGTGTTTGAAGGAAGCAAGTTTGCAATGCACCAGTTACACATCCTGGTGTACA  >  NZ_CP034943/3274053‑3274329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: