BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,306	G→T	5.5%	N138K (AAC→AAA)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,306	0	G	T	5.5%	706.4 / 6.5	248	N138K (AAC→AAA)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base G (155/70); new base T (2/21); total (157/91)
Fisher's exact test for biased strand distribution p-value = 1.77e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CATTTATCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGT  >  NZ_CP034943/3280167‑3280444

cATTTATCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGTTGTATTCg                                                                                                                                            >  2:1879609/1‑140 (MQ=255)

 aTTTATCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGt                                                                                                                                           >  1:1709310/1‑140 (MQ=255)

   ttATCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGttt                                                                                                                                         >  1:780604/1‑140 (MQ=255)

      tCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTcc                                                                                                                                      >  1:3704124/1‑140 (MQ=255)

        aaaTAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCaa                                                                                                                                    >  2:2040399/1‑140 (MQ=255)

           tAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAAttt                                                                                                                                 >  2:1420417/1‑140 (MQ=255)

           tAGAAAACAGATGTCGCACGAACACTAACGTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAATTCGGTCGGTGTATTCGTTTTCGAAttt                                                                                                                                 >  2:4934308/1‑140 (MQ=255)

                aaCAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaa                                                                                                                            <  1:3489100/140‑1 (MQ=255)

                aaCAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaa                                                                                                                            <  1:1189849/140‑1 (MQ=255)

                aaCAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaa                                                                                                                            <  2:3523285/140‑1 (MQ=255)

                aaCAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaa                                                                                                                            <  1:1358876/140‑1 (MQ=255)

                aaCAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaa                                                                                                                            >  2:4571987/1‑140 (MQ=255)

                   aGATCTCGCACGAACACTACCTTCGTCAGACTATCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTAAATATAACATGCGGAATTTAATGTAAAAGACAGTCGGTCGGTGTATTCGTTTTCCAATTTTCtaatta                                                                                                                         <  2:4641785/140‑1 (MQ=255)

                        tCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTgg                                                                                                                    <  2:4704452/140‑1 (MQ=255)

                              taaaaatAACTTATTCATATTTTCCATCTGTGCTGTACAACAAGTAAGACCGGTCACCCTTAATATAACAACCGGAATTTAATTTAAAAGAAAGTCTGTCGTTGTATTAGTTTTCCAATTTTCTAATTAATTGGTAGAGc                                                                                                              <  1:852869/134‑1 (MQ=255)

                              gAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGc                                                                                                              <  2:2283338/140‑1 (MQ=255)

                              gAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGc                                                                                                              <  1:461094/140‑1 (MQ=255)

                              gAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGc                                                                                                              <  2:1457119/140‑1 (MQ=255)

                              gAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGc                                                                                                              <  2:3864958/140‑1 (MQ=255)

                               aaCACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGcc                                                                                                             >  1:1003361/1‑140 (MQ=255)

                               aaCACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGcc                                                                                                             >  1:310899/1‑140 (MQ=255)

                                    tAACTTCTTCAGATTTTCCATCTGTGCTGTACACCAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTACAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCa                                                                                                        <  2:2668322/140‑1 (MQ=255)

                                        ttcttcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAAt                                                                                                    <  1:4085193/140‑1 (MQ=255)

                                          cttcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCa                                                                                                  >  1:2632645/1‑140 (MQ=255)

                                            tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                >  1:3263117/1‑140 (MQ=255)

                                            tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                >  1:2165824/1‑140 (MQ=255)

                                            tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                >  1:4715513/1‑140 (MQ=255)

                                            tcAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAac                                                                                                >  1:2586095/1‑140 (MQ=255)

                                                  tttCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGc                                                                                          >  1:363182/1‑140 (MQ=255)

                                                         ctttgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTg                                                                                   <  2:2384799/137‑1 (MQ=255)

                                                         cTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTg                                                                                   <  2:258169/140‑1 (MQ=255)

                                                            tgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTaa                                                                                <  2:2949327/140‑1 (MQ=255)

                                                            tgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTaa                                                                                >  1:1612203/1‑140 (MQ=255)

                                                            tgCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTaa                                                                                >  2:449175/1‑140 (MQ=255)

                                                               tGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaat                                                                             <  1:3229579/140‑1 (MQ=255)

                                                               tGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAaat                                                                             <  1:1421475/140‑1 (MQ=255)

                                                                     acaaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGt                                                                       <  2:780604/140‑1 (MQ=255)

                                                                      caaGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGTGCCACTCAAAATCAACACGAGCCTGTTTGTAAATTGATTGtg                                                                      >  1:3023215/1‑139 (MQ=255)

                                                                           acgCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  1:5107770/3‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACCAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACTAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  2:3076079/1‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGACTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  2:824287/1‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 <  1:741285/140‑1 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  1:4027350/1‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  2:4068010/1‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  2:102523/1‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 <  2:1207640/140‑1 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  2:3740627/1‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  1:2803274/1‑140 (MQ=255)

                                                                           aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                 >  2:3948667/1‑140 (MQ=255)

                                                                            aGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaa                                                                >  1:305818/1‑140 (MQ=255)

                                                                                  gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCATAATCAACACGAGCCTTTTTGTAAGAGGAAGGGTGAGTAATAGATa                                                          >  1:2888775/1‑140 (MQ=255)

                                                                                  gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                          >  1:2690096/1‑140 (MQ=255)

                                                                                  gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                          >  2:525472/1‑140 (MQ=255)

                                                                                  gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                          >  1:5025424/1‑140 (MQ=255)

                                                                                  gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                          >  2:267692/1‑140 (MQ=255)

                                                                                        cTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAt                                                    >  2:3269684/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTACAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  2:3318534/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTACAACAAAGTAGGTCGGTGTATTCGGGTTCGAATTTTCTCAGTAATTGGTAGAGGCACTCAAAATCAACACGAGCCTTTTTGTAAAATGACTGGTGATTAAAAGATAATTGATTTTTATCAg                                           >  1:1278307/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCCATTTTCTAATTAATTGGTAGAGCCTCTCAAAATCAACACGAGCCTTTTTGTACAATGAAGGTTGATTAAAAGATAAGTGATTGTTATCat                                           >  1:2197032/1‑139 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                              >  1:957762/1‑121 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  2:3689942/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  1:1023957/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  2:3184862/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  2:5025067/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  2:5146288/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  1:682267/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  1:4512885/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  1:2285080/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                           >  2:2243071/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATAAg                                           >  2:2983221/1‑140 (MQ=255)

                                                                                                 aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGGTGATTAAAAGATAAGTGATTTTTATCAg                                           >  1:4284072/1‑140 (MQ=255)

                                                                                                     tcgtaaTTTAATGTAAAAGAAATTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTATTTGGTAGAGCCACTCAAAATCAACACGAGCATTTTTGTAAAATTAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                       <  2:1003361/136‑1 (MQ=255)

                                                                                                     gtgtattttAATGTAAAATAAAGTGTGTCGGTTTATTCTTTTTACAATTTTATAATTATTTGGTAGAGCCAATAAAAATCAACACGAGCCTTTTTTTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCAGaaaa                                       <  2:3487574/134‑1 (MQ=255)

                                                                                                     ggggAATTTCATGTAAATGAAAGCGGGGCGGTTTATTCGTTTTCCAATTTTCTATTTAATTGTTAGCGCCACGCAAAATCAACACGCGCCTTTTTGTAAAATGAATGTTTATTAAAATATATGTGTTTTTTATCAGaaca                                       <  2:291385/138‑3 (MQ=255)

                                                                                                     gcgtattttaatttaaAACAAATTCTGTCGGTTTATTCGTTTTCCAATTTTCTAATTATTTGTTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTTATTAAAAGATAAGTTATTTTTATCAGaaaa                                       <  2:3132561/134‑1 (MQ=255)

                                                                                                     gCGTAATTTAATTTAAAATAAAGTGGGTCGGTTTATTCTTTTTCCAATTTTCTAATTATTTGGTAGAGCCACTCAAAATCAACCCGCGCGTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                       <  2:310899/140‑1 (MQ=255)

                                                                                                     gCGTAATTTAATTAAAAATAAAGTGGGTCGTTGTATTGTTTTTCAAATTTTCTATTTAATTGGTAGAGCCACTAAAAATCAAAACGAGCCTTTTTGTAAAATGAATGTTTATTAAAACATAAGTGATTTTTATCACaaaa                                       <  2:1709310/140‑1 (MQ=255)

                                                                                                     gCGTAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCAACTCAAAATCAACACGAGCGTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                       <  2:3625460/140‑1 (MQ=255)

                                                                                                     gCGGATTTTAATTTAAAAGAAAGTCGTGCGGTTTTTTCTTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAATTGAATGTTGATTAAAAGATAAGTGATTTTTATCTGaaaa                                       <  2:4832523/140‑1 (MQ=255)

                                                                                                     gCGGATTTTAATTTAAAAGAAAGTCGGTGGTTTTATTCTTTTTCCATTTTTCTAATTAATTGTTAGAGCCACTCAAAATAAACACGAGCATTTTTGTAAAATGATTGTTTATTAAAAGATAAGTGATTTTTATAAGaaaa                                       <  2:4715513/140‑1 (MQ=255)

                                                                                                     gCGGAATTTAATTTAAAAGAAAGTCGTTGGGTTTTTTCTTTTTCAAATTTTATAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAATGATACGTTATTTTTATAAGaaca                                       <  2:2430592/140‑3 (MQ=255)

                                                                                                     gCGGAATTTAATTTAAAACAACTGGGGTCGTTTTTTTCTTTTTCAATTTTTCTAATTAATTGGTAGACCCACTAAAAATCAACACGAGCCTTTTTTTAAAATTAATTTTGATTAAAACATAAGTTATTTTTATCACaaaa                                       <  2:4512885/140‑1 (MQ=255)

                                                                                                     gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTACAATGACTGTTGATTAAATGATAAGTGATTTTTCTCAgtaca                                       >  1:3553715/1‑136 (MQ=255)

                                                                                                     gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                       >  1:1540753/1‑140 (MQ=255)

                                                                                                     cgtcattttaatttaaAAGAATGTGGGTCGTTGTATTCTTTTTCAATTTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACCAGCCTTTTTTTAAAATTAATGTTGATTAAAAGATAAGGGATTTTTATCAGaaaa                                       <  2:4052386/134‑1 (MQ=255)

                                                                                                     cggtaaTTTAAGTTAAAACAAAGTCGGTGGTTGTATTCGTTTTCAAATTTTGTAATTAATTGTTAGTGCCACACAAAATCAACACGAGCCTTTTTGTAAAATTAATTTTGATTAAAATATAAGTTATTTTTATCAGaaaa                                       <  2:3454218/136‑1 (MQ=255)

                                                                                                     caaatatttaatttaAAAGAAAGTCGGTCGGTTTATTCTTTTTCAAATTTTATAATTATTTGGTAGAGCCAATAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTTATTTTTATAACaaaa                                       <  2:2621542/135‑1 (MQ=255)

                                                                                                                   aaaaTAAAGTCTGTCGTTTTATTCTTTTTACATTTTTCTAATTAATTGGTAGAGCAACTCAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                         <  2:4221447/140‑1 (MQ=255)

                                                                                                                     aagaaagTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACt                       >  2:1899559/1‑140 (MQ=255)

                                                                                                                     aagaaagTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACt                       >  2:209830/1‑140 (MQ=255)

                                                                                                                       gaaagTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTga                     >  2:155798/1‑140 (MQ=255)

                                                                                                                       gaaagTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGAGATTTTTATCAGAAAACGACCCTATACCGACTga                     >  2:5172839/1‑140 (MQ=255)

                                                                                                                          agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTgaaga                  >  1:587782/1‑140 (MQ=255)

                                                                                                                            tcggtcggTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaa                >  2:158806/1‑140 (MQ=255)

                                                                                                                              tgtcggtGTATTCGTTTGCAAATTTTCTAATTAATGGGTAGAGCAAAAAAAAATCAACACTAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATCACAAAACGACCCTATACCGACTGAAGaaaaa              <  2:4259556/139‑1 (MQ=255)

                                                                                                                               gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAAGGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa             >  1:2390869/1‑140 (MQ=255)

                                                                                                                               gtcggtGTATTCGTTTTCCAATGTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa             >  1:4871419/1‑140 (MQ=255)

                                                                                                                                      ttttCGTTTTCAAATTTTCTAATTAATTGTTAGAGCCACTAAAAATCAACACGAGCATTTTTTTAAAATGAATGTTGATTAAAAGATACGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa      <  2:4027350/138‑1 (MQ=255)

                                                                                                                                      tATTCGTTTTCAAATTTTATAATTAATTGGTAGAGCCACTCAAAATCAAAACGAGCTTTTTTGTAAATTTAATGTTGTTTAAAAGATAATTGATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa      <  2:1691359/140‑1 (MQ=255)

                                                                                                                                        ttCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAg    >  1:1317799/1‑140 (MQ=255)

CATTTATCAAATAGAAAACAGATGTCGCACGAACACTAACTTCTTCAGATTTTCCATCTGTGCTGTACAACAAGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGT  >  NZ_CP034943/3280167‑3280444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`