BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,647	C→A	41.8%	V252L (GTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,647	0	C	A	41.8%	604.0 / inf	812	V252L (GTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base C (265/207); new base A (211/128); total (477/335)
Fisher's exact test for biased strand distribution p-value = 8.34e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 823 total aligned reads displayed.

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285508‑3285785

tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                                                                                            <  1:2800112/140‑1 (MQ=40)

tAAGATGTATACACAGAACCTTGGTCACTATGTAACACGAAGTTCTCAGTCAGTGTTGGCAGCTTATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAc                                                                                                                                            <  1:258082/140‑1 (MQ=255)

     tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTa                                                                                                                                       <  2:3171974/140‑1 (MQ=255)

     tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                                                                                                       <  2:4226480/140‑1 (MQ=255)

       tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAat                                                                                                                                     >  2:1390890/1‑140 (MQ=255)

       tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAat                                                                                                                                     >  1:2282956/1‑140 (MQ=255)

           cacaGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATAc                                                                                                                                 <  2:3869016/140‑1 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                              >  1:4526710/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                              >  1:840747/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                              >  1:4091914/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACCCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                              >  1:2626201/1‑140 (MQ=11)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAatat                                                                                                                                   >  1:2349314/1‑134 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGa                                                                                                                             >  1:4974918/1‑140 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGa                                                                                                                             >  1:4443522/1‑140 (MQ=255)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGa                                                                                                                             >  1:1472248/1‑140 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTATTATACTGGaa                                                                                                                            <  1:936115/140‑1 (MQ=11)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                            <  2:3936954/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                            <  1:3354960/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                            >  2:1892916/1‑140 (MQ=255)

                aaCCTTGGTAACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGACGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCACTCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                            <  1:2985998/140‑1 (MQ=11)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAgg                                                                                                                         <  2:2433010/140‑1 (MQ=255)

                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGtc                                                                                                                       >  1:4259047/1‑139 (MQ=255)

                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTa                                                                                                                       >  1:2456123/1‑140 (MQ=255)

                      ggTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTAc                                                                                                                      >  1:636641/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGt                                                                                                                >  1:2237349/1‑140 (MQ=255)

                             aTGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGtt                                                                                                               <  2:3591962/140‑1 (MQ=255)

                             aTGTAACACGCAGGTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGtt                                                                                                               <  2:3754197/140‑1 (MQ=255)

                              tGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCCATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGttt                                                                                                              <  2:2835118/140‑1 (MQ=11)

                                   cacGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTc                                                                                                         >  2:5093810/1‑140 (MQ=255)

                                     cGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCa                                                                                                       <  2:873836/140‑1 (MQ=255)

                                     cGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCa                                                                                                       <  2:1669827/140‑1 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  1:2702710/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  1:3696809/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                            <  1:1217483/118‑1 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  2:208136/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                      >  2:3358607/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACACAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTCATATACTGGAAAGGTACACTTGTTTCTGTCCATaa                                                                                                    >  1:5210025/1‑140 (MQ=11)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACaa                                                                                                                    <  2:4972388/124‑1 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                    >  2:365980/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                    >  2:1461760/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                    >  2:2331388/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                    >  2:2271826/1‑140 (MQ=255)

                                          ttCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                                                                                  <  2:254109/140‑1 (MQ=255)

                                          ttCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                                                                                  <  2:4385113/140‑1 (MQ=255)

                                            ctcAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                <  1:1088020/140‑1 (MQ=255)

                                            ctcAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                <  2:4543893/140‑1 (MQ=255)

                                            ctcAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa                                                                                                >  1:3057437/1‑140 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                        >  1:2836896/1‑140 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                        >  1:484051/1‑140 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                        >  1:4608418/1‑140 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                        >  2:4709806/1‑140 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                        >  2:1049959/1‑140 (MQ=255)

                                                            aGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGCCAAATATGTGATGTCCGt                                                                                >  2:173557/1‑140 (MQ=255)

                                                               tgatgaAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                             >  2:3093268/1‑140 (MQ=12)

                                                                gATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACt                                                                            <  1:3630668/140‑1 (MQ=12)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCa                                                                   <  2:4117845/140‑1 (MQ=40)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                   <  1:4189264/140‑1 (MQ=21)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                   >  1:3790838/1‑140 (MQ=21)

                                                                                   aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                         <  1:3230634/140‑1 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:2698455/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:4579368/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  1:1721241/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:647229/1‑140 (MQ=21)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  1:2003430/1‑140 (MQ=21)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:1258047/1‑140 (MQ=21)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  1:1310240/1‑140 (MQ=21)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  1:1000835/1‑140 (MQ=21)

                                                                                     aGTCTGTGTCCTGATTATCCCCAATCGTAAAAGAACTCACTTCTCCATTTTCTACATCTAATATACTGGAAAGGGACAATTGTTTCTGTCAATACGTGAAATATGTGATGTCCGGTCCTAGTTTTTCAAGAGGGCGagaa                                                       >  2:2933100/1‑137 (MQ=14)

                                                                                           tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGa                                                 >  1:4675748/1‑140 (MQ=40)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           >  1:4346130/1‑140 (MQ=40)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           <  1:1625594/140‑1 (MQ=40)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                           >  1:1822632/1‑140 (MQ=21)

                                                                                                   ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  1:3166017/140‑1 (MQ=40)

                                                                                                   ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                         <  2:4824185/140‑1 (MQ=21)

                                                                                                     aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  1:1664819/1‑140 (MQ=38)

                                                                                                     aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAATTCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  1:2879939/1‑140 (MQ=16)

                                                                                                     aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  1:5160808/1‑140 (MQ=18)

                                                                                                     aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                       >  2:4644243/1‑140 (MQ=18)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  1:2077770/140‑1 (MQ=37)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  1:2109728/140‑1 (MQ=37)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                      <  1:1397235/140‑1 (MQ=37)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                     >  1:5068180/1‑140 (MQ=14)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                     >  2:2210568/1‑140 (MQ=9)

                                                                                                        cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  2:680222/140‑1 (MQ=35)

                                                                                                        cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                    <  2:2003430/140‑1 (MQ=11)

                                                                                                          cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  1:4742725/140‑1 (MQ=34)

                                                                                                          cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  1:3573204/140‑1 (MQ=34)

                                                                                                          cAATCCTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                  <  1:192106/140‑1 (MQ=21)

                                                                                                            aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAatat                                >  1:558070/1‑140 (MQ=34)

                                                                                                                  aaaGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATc                          <  1:751090/140‑1 (MQ=32)

                                                                                                                     gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGAc                       <  2:4024057/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:4018241/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:3490716/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:755005/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:3109460/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  1:3910811/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:2642889/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATATAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGTCAAATATGTGATGTCCTTTACTAGTTTTTCACGAGGGCGATCATACTGAAAGTTCCGATCCAATATATTATCGACCa                     <  2:1184625/140‑1 (MQ=11)

                                                                                                                                     tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc       >  1:3448420/1‑140 (MQ=32)

                                                                                                                                     tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc       >  2:5240792/1‑140 (MQ=32)

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285508‑3285785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`