BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,713	G→A	29.4%	L230L (CTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,713	0	G	A	29.4%	581.4 / 289.3	351	L230L (CTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base G (110/136); new base A (52/51); total (163/188)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 352 total aligned reads displayed.

TTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285574‑3285785

      aTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTc                                                                     >  2:3166017/1‑140 (MQ=40)

       tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                    >  2:762602/1‑140 (MQ=21)

         tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                  >  1:51232/1‑140 (MQ=40)

         tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                  >  1:1665219/1‑140 (MQ=40)

              gACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGc                                                             >  1:4092098/1‑140 (MQ=21)

                 aaaGTCTGTGTCCTTCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  2:3287943/140‑1 (MQ=255)

                 aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                          <  2:102852/140‑1 (MQ=40)

                 aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                          <  2:2352928/140‑1 (MQ=40)

                 aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  2:1885680/140‑1 (MQ=21)

                 aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          >  2:4668561/1‑140 (MQ=21)

                 aaaGTCTGTGTCCTGCTTATCCCCAATAGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  1:3122442/140‑1 (MQ=12)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAATGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCGGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:434868/1‑140 (MQ=255)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  1:1721241/1‑140 (MQ=40)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  1:3676517/1‑140 (MQ=40)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  2:2698455/1‑140 (MQ=40)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAATTCTCATATACTGTAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  2:4573650/1‑140 (MQ=11)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:1000835/1‑140 (MQ=21)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  2:2012794/1‑140 (MQ=21)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:2757992/1‑140 (MQ=21)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:1310240/1‑140 (MQ=21)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  2:647229/1‑140 (MQ=21)

                   aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                        >  1:1979258/1‑140 (MQ=21)

                   aGTCTGTGTCCTGCTTATACCCAATAGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                        >  2:445695/1‑140 (MQ=255)

                   aGTCTGTGTCCTGATTATCCCCAATCGTAAAAGAACTCACTTCTCCATTTTCTACATCTAATATACTGGAAAGGGACAATTGTTTCTGTCAATACGTGAAATATGTGATGTCCGGTCCTAGTTTTTCAAGAGGGCGagaa                                                        >  2:2933100/1‑137 (MQ=14)

                        gtgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                          <  2:368978/133‑1 (MQ=21)

                         tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑a                                                 >  1:4675748/1‑140 (MQ=40)

                         tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑a                                                 >  2:727119/1‑140 (MQ=21)

                               gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                           >  2:1397235/1‑140 (MQ=40)

                               gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                           <  1:2327702/140‑1 (MQ=40)

                               gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                           <  2:2511723/140‑1 (MQ=40)

                               gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                           >  1:3933130/1‑140 (MQ=40)

                               gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                           <  2:4092098/140‑1 (MQ=21)

                               gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                           <  1:341636/140‑1 (MQ=21)

                                 ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCg                                         <  1:240205/140‑1 (MQ=40)

                                 ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCg                                         <  1:4614247/140‑1 (MQ=40)

                                 ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCg                                         <  1:3022591/140‑1 (MQ=21)

                                   aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGGTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGAt                                       >  1:2021760/1‑140 (MQ=38)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:1359533/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:1249946/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:951698/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:3525722/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:2271826/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:2109728/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:208186/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:4596571/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:551450/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:1397235/140‑1 (MQ=37)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:3769844/140‑1 (MQ=17)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:3752103/140‑1 (MQ=17)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:3927092/140‑1 (MQ=17)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:1487298/140‑1 (MQ=17)

                                    tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:594708/140‑1 (MQ=17)

                                    tCCCCAATCGTAAAAGCAATCAATTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGCCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  1:2678319/140‑1 (MQ=12)

                                    tCCCCAATCGTAAAAGCAATAAATTCTCAATTGTATAAATCTAATATACTGTAAATGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                      <  2:4140797/140‑1 (MQ=11)

                                     ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                     >  2:2210568/1‑140 (MQ=9)

                                      cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                    <  2:921336/140‑1 (MQ=35)

                                      cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                    <  2:4251751/140‑1 (MQ=11)

                                      cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                    <  1:2828548/140‑1 (MQ=11)

                                      cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                    <  1:1831848/140‑1 (MQ=11)

                                      cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                    <  2:2626201/140‑1 (MQ=11)

                                      cccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                    <  2:2439626/140‑1 (MQ=11)

                                          aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTCGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                                >  1:1258786/1‑140 (MQ=21)

                                          aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                                >  1:2480060/1‑140 (MQ=34)

                                          aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                                >  1:3597077/1‑140 (MQ=34)

                                          aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                                >  2:2905719/1‑140 (MQ=34)

                                          aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                                >  1:1644608/1‑140 (MQ=34)

                                          aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                                >  1:558070/1‑140 (MQ=34)

                                          aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                                >  1:4541211/1‑140 (MQ=34)

                                                aaaGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATc                          <  1:751090/140‑1 (MQ=32)

                                                   gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGAc                       <  2:4024057/140‑1 (MQ=32)

                                                   gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGAc                       <  1:4735303/140‑1 (MQ=32)

                                                   gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGAc                       <  2:1710717/140‑1 (MQ=32)

                                                   gCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGAc                       <  2:265104/140‑1 (MQ=32)

                                                     actCACTTCTCCATTGTATACCTCTACTATACTGGAAAGGTACAAGTGTTTCTGTCCCTAATGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATGTATTATCGACCa                     <  1:1144685/138‑1 (MQ=11)

                                                     aaTCACTTCTCCATTGTATACATCTTATATACTGGAACGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACGG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  1:1519372/140‑1 (MQ=12)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  2:1444233/140‑1 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  1:3084590/140‑1 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  1:1765755/140‑1 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  2:3453531/140‑1 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  1:3318413/140‑1 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  1:3910811/140‑1 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  1:4129116/140‑1 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     >  2:2079258/1‑140 (MQ=32)

                                                     aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTAAAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  1:2595785/140‑1 (MQ=17)

                                                     aaTCACTTCTCCATTGTATACATCTAAGATACTGGAAAGGTACAATTGTTTCTGTCCATACGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  2:5191410/140‑1 (MQ=17)

                                                     aaTCACTTCTCCATTGTATACATATAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGTCAAATATGTGATGTCCTTTACTAGTTTTTCACGAGGGCGATCATACTG‑AAAGTTCCGATCCAATATATTATCGACCa                     <  2:1184625/140‑1 (MQ=11)

                                                      aTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCAc                    >  1:3132192/1‑140 (MQ=32)

                                                      aTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCAc                    >  1:4314948/1‑140 (MQ=32)

                                                      aTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTAAAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAAGTTCCGATCCAATATATTATCGACCa                     <  1:1045151/140‑1 (MQ=12)

                                                          cTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCa                <  1:2208166/140‑1 (MQ=32)

                                                          cTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCa                <  1:3566984/140‑1 (MQ=32)

                                                             ctcCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCatat             >  1:5188174/1‑140 (MQ=32)

                                                               ccATTGTATACATATAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATgg           <  2:4700955/140‑1 (MQ=17)

                                                                 aTTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCt         <  2:484051/140‑1 (MQ=32)

                                                                   tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc       >  1:3223753/1‑140 (MQ=32)

                                                                   tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc       >  2:3552320/1‑140 (MQ=32)

                                                                   tGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGc       >  1:423144/1‑140 (MQ=32)

                                                                     tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc     >  1:724700/1‑140 (MQ=32)

                                                                       taCATCTAATATACTGGAAAGGTCCAATTGTTTCTGTGCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt   >  1:5012735/1‑140 (MQ=17)

                                                                       taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt   >  1:4925526/1‑140 (MQ=32)

TTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCATT  >  NZ_CP034943/3285574‑3285785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`