Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,970 G→T 21.0% Q206K (CAA→AAA)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9700GT21.0% 645.9 / 64.8 358Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (99/173);  new base T (83/2);  total (183/175)
Fisher's exact test for biased strand distribution p-value = 7.77e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 371 total aligned reads displayed.

CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108
                                                                                                                                           |                                                                                                                                          
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCCTTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTCATTTTTTg                                                                                                                                            >  2:295650/1‑140 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTg                                                                                                                                            >  2:545546/1‑140 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTg                                                                                                                                            >  1:3060369/1‑140 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTg                                                                                                                                            >  2:3505933/1‑140 (MQ=255)
 tATTGTAAGTGACACGGCAGACTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTATTATTATACTATCTCATTTCTACCCAGACATTTATAATGGCGACCACAACGGCTTTCGTCCGGTCGGTTACATTCATTATTTGc                                                                                                                                           >  2:4794506/1‑140 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:4708158/140‑1 (MQ=255)
   ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCaa                                                                                                                                         >  2:3137833/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAaaat                                                                                                                                    >  1:3651829/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATAATTTTTTTAaaaaaaa                                                                                                                                    >  1:2127312/1‑137 (MQ=255)
         gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAtt                                                                                                                                   >  2:3307382/1‑140 (MQ=255)
           gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGGAAAATATTgg                                                                                                                                 >  1:5224036/1‑139 (MQ=255)
           gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGGCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTGCAAAAAATTGc                                                                                                                                 >  2:2002407/1‑140 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACCATAATTTTTTTCAAAATAATggta                                                                                                                               >  1:837355/1‑137 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTc                                                                                                                               >  2:687785/1‑140 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTtctc                                                                                                                               >  2:1066096/1‑140 (MQ=255)
                          aaaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCa                                                                                                                  >  1:2423904/1‑140 (MQ=255)
                           aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                 <  1:4640168/140‑1 (MQ=255)
                               gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTGTCACATTGTTTTTAACGGtt                                                                                                             >  1:1167680/1‑140 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  1:1938506/140‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  1:1043739/140‑1 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTAACATGGTTTTTAACGGTTTtct                                                                                                         >  2:620212/1‑140 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATATCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCCCGGTTTtct                                                                                                         <  2:179017/140‑1 (MQ=255)
                                    gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCAAATGGTTTTTAACGGTTTtctc                                                                                                        >  2:4877760/1‑140 (MQ=255)
                                    gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtctc                                                                                                        >  2:3429806/1‑140 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACGCAGCCATTTTTAATGGCGACCACAAAGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                       <  2:2340777/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTAAAATGGTTTTTCACGGttttataa                                                                                                       >  2:171812/1‑136 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCAATTTTTGCAAAATAATGCTCACATTGTTTTTCACGGTTTTCTCa                                                                                                       >  1:1258872/1‑140 (MQ=255)
                                         gTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGa                                                                                                   <  2:4711500/140‑1 (MQ=255)
                                          tAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGAt                                                                                                  <  1:2735387/140‑1 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                             >  2:1274918/1‑140 (MQ=255)
                                                  aaGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTGCGGTCGTTTACATTCATTTTTTGCAAAAGGGTTCTCACATGGGTGTTGACGGTTTTCTCACTGGTATACAATg                                                                                          >  2:3627574/1‑140 (MQ=255)
                                                  aaGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCATTTTTTTCAAAAAATTTGTAACATGGTTTTTCAAGGGTTTTTCAATGATAAACAATg                                                                                          >  1:3242310/1‑140 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCGACCCAGCCATTTTTAATGGCGACCACAACGGCTGGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  1:4922478/140‑1 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATATCTCAAATGGTTTTTAACCGTTTTATCACTGATAAAAAATGAt                                                                                        >  2:4165475/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTGCTCACATGGTTTTTCACGGTTTTAACACTGATAAACAATGAt                                                                                        >  2:3922919/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTGACGGTTTTGTGACTGATAAACAATGAt                                                                                        >  1:1356024/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACAAGGTTTTTAAAGGTTTTATAACAGATAAAAAATGAt                                                                                        >  1:4230144/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACAATGTTTTTAACGGTTTTGTCACTGATaaaaaaatat                                                                                        >  1:4377872/1‑136 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                        >  2:2138342/1‑140 (MQ=255)
                                                     tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                       <  1:1885919/140‑1 (MQ=255)
                                                     tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                       <  1:73573/140‑1 (MQ=255)
                                                        ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAc                                                                                    <  1:4954780/140‑1 (MQ=255)
                                                          tattatACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGGAAAAAATTTCTCACAAGGTTTTTAACGGTTTTCTCACTGATAAACAAAGATTCACCa                                                                                  >  1:5088704/1‑140 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACAAGGTTTTTCACCGTTTTCTCACTGATAAAAAAAGATTCAACAATAcc                                                                             >  2:2744039/1‑140 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTTCTCACAAGGTTTTTCAAGGTTTTATAAATTATAAAAAAAGATTAACCaaaaac                                                                             >  1:3500844/1‑136 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATAACTGATAAAAAAAGATTAAACAAAAcc                                                                             >  2:4113878/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATAATTCTCAAATTGTTTTTGAAGGTTTTGTGACTTATAAACAATTATTGACCAAAAACGCgg                                                                         >  1:3455599/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                            >  2:2245901/1‑89 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCGCACATGGTTTTTCACCGTTTTCTCAAGGATAAAAAAAGATTAACCAATAACGCgg                                                                         >  1:4278297/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTATCACTGATAAACAATTATTCACCAATAACGCgg                                                                         >  2:1570709/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAAATTCTGAAAAGGTTTTTAAAGGTTTTTTCAATGATAAAAAATGATTAAACAATAcccccg                                                                         >  1:151768/1‑136 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTTCAAAATATTGCTCACATGGTTTTTCAAGGTTTTCTCACTGATAAACAATGATTAAACAATACCGCgg                                                                         >  2:2888361/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTGCAAAAAATTGCTCACATGGTTTTTCACGGTTTTCGCAATTAAAAACAATTATTCAACAAAACCACgg                                                                         >  2:2128597/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAACGGTTTTATCACTTATAAACAAAGATTAACCAAAACCGCgg                                                                         >  2:845162/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAGATACAATTTTTTCAAAATAATTGTCACATGGTTTTTAACGGTTTTGTGACAGATAAACAATGCTTCACCCATACAGCgg                                                                         >  1:3170717/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAAAAATGATTCACCAATAAAGCGGt                                                                        >  2:3999296/1‑140 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAAAGATTAACCCAAACCGCGGTTTCtt                                                                   >  1:3033892/1‑140 (MQ=255)
                                                                            cTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCttttt                                                                <  2:4509335/140‑1 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                              <  1:4012896/140‑1 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAAAACGCGGTTTCtttttac                                                              >  2:3744977/1‑138 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAAGGTTTTTCACGGTTTTCTAACTGATAAACAAAGATTAACCAAAAACGCGGTTGCtttttac                                                              >  2:783555/1‑138 (MQ=255)
                                                                               cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCGCACATTGTTTTTCACGGGTTTGTCACTGATAAACAATGATTCACCAATAACCCGGTTGCTTTTTGCg                                                             >  1:3593985/1‑140 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  1:2655751/140‑1 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  2:3186105/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCTTTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                    <  1:4169300/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                    <  1:285732/140‑1 (MQ=255)
                                                                                        ttctAACGGCGACCACAACGGCGTGCGTCCGGACGTTTACATTCATGTATTGCAAAATATTGCTCACAACCTTTTTCACGGTTTTCTCACTGATAAACAATGAGTCACCACGACCGCGGGTGCGTTTTCCGTCTGCAAGc                                                    <  1:2552439/137‑1 (MQ=255)
                                                                                             aTGGCGACCACAACGGCTTTCGTACGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGTTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTg                                               <  1:2002407/140‑1 (MQ=255)
                                                                                              tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATTATTAACCAATAACGCGGTGTCGTTTTCCGTCTGCCAGCATCttc                                              >  2:519268/1‑138 (MQ=255)
                                                                                              tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                              <  1:4836223/140‑1 (MQ=255)
                                                                                                gCGACCACCACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  2:5066626/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:2111701/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  1:171812/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTGTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCCCCTATACCGCGGTTGCTTTTTCCGTCGGCAAGCATCTgcag                                            <  1:984494/140‑1 (MQ=255)
                                                                                                 ggACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                           <  1:3382861/139‑1 (MQ=255)
                                                                                                          cGGCTTGCGTCCGGTCGTTTACATTGATTTTTTTGAAAAAATTTCTCACAATGTTTTTAAAGGTTTTATAACTGATAAAAAAATATTCACCAAAAACCCCGTTGCTTTTTTCGTCTGCAAGCAAATGCAGCACATaacaa                                  >  1:1618097/1‑139 (MQ=255)
                                                                                                               tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAACAATAACGCGGTTGCGTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTAccg                             >  2:4051268/1‑140 (MQ=255)
                                                                                                               tGCGTCCGGTCGTTTACAATCATTTTTTGCAAAAAATTGCTCACAATGTTTTTCACGGTTTTGTAACTTATAAACAAAGATTAACCAAAAACCCGGTTGCTTTTTCCCTATGAAATAATAAGCAGCAATTCACAttaacg                             >  2:4380341/1‑136 (MQ=255)
                                                                                                                 cGTCCGGTCGTTTACCTTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                           <  2:919666/140‑1 (MQ=255)
                                                                                                                  gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgccg                          <  1:2794692/140‑1 (MQ=255)
                                                                                                                   tCCGGTCGTTTACATTCATTTTTTGCAAAATATGGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGa                         <  1:2185177/140‑1 (MQ=255)
                                                                                                                      ggTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                      <  1:1447460/140‑1 (MQ=255)
                                                                                                                       gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                     <  2:2423904/140‑1 (MQ=255)
                                                                                                                       gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTGTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                     <  2:4551847/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:494777/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:656910/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:4053547/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:1356024/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:1145215/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTaaa                   <  1:3740330/140‑1 (MQ=255)
                                                                                                                           tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  2:277501/140‑1 (MQ=255)
                                                                                                                           tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAAACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  2:1877690/140‑1 (MQ=255)
                                                                                                                                attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa            <  1:1748729/140‑1 (MQ=255)
                                                                                                                                attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa            <  2:2565780/140‑1 (MQ=255)
                                                                                                                                    attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg        >  2:209742/1‑140 (MQ=255)
                                                                                                                                     ttttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGt       <  1:384690/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:4458136/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  >  1:1190490/1‑140 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:2283754/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:4230144/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: