Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,987 T→A 7.0% H200L (CAT→CTT)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9870TA 7.0% 1151.2 / 6.8 399H200L (CAT→CTT) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (123/238);  new base A (36/2);  total (159/240)
Fisher's exact test for biased strand distribution p-value = 9.03e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

CAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCT  >  NZ_CP034943/3446849‑3447125
                                                                                                                                          |                                                                                                                                          
cAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAAAATTGCTCACATg                                                                                                                                           >  2:4095598/1‑140 (MQ=255)
   ccTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGtt                                                                                                                                        >  2:3749185/1‑140 (MQ=255)
         aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                  <  1:1886732/140‑1 (MQ=255)
             gACCTGCCCAGTAAAAGGCAAGTCTCTTCTTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTGAAAATATTGGTCACATGGTTTTTCAAGGtt                                                                                                                              >  2:3204440/1‑140 (MQ=255)
              aCCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGttt                                                                                                                             >  2:489562/1‑140 (MQ=255)
                 tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                          <  1:1043739/140‑1 (MQ=255)
                  gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCAAATGGTTTTTAACGGTTTtctc                                                                                                                         >  2:4877760/1‑140 (MQ=255)
                  gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtctc                                                                                                                         >  2:3429806/1‑140 (MQ=255)
                  gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtctc                                                                                                                         >  2:656910/1‑140 (MQ=255)
                       gTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGa                                                                                                                    <  2:4711500/140‑1 (MQ=255)
                        tAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGAt                                                                                                                   <  1:1457765/140‑1 (MQ=255)
                        tAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGAt                                                                                                                   <  1:2735387/140‑1 (MQ=255)
                        tAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAAGGTTTTTCACGGTTTTCTCACTGAt                                                                                                                   >  2:552542/1‑140 (MQ=255)
                            aGGCAAGTCTCCTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGTAAAATATTGCTGACATGGTTTTTAACGGTTTTCTCACTGATAAAc                                                                                                               <  2:5189662/140‑1 (MQ=255)
                                aaGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTGCGGTCGTTTACATTCATTTTTTGCAAAAGGGTTCTCACATGGGTGTTGACGGTTTTCTCACTGGTATACAATg                                                                                                           >  2:3627574/1‑140 (MQ=255)
                                 aGTCACTTATTATACTATCTCAGTTCTTCCCAGCCATTTTGAATGGCGACCCCAACCGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                          <  1:1210006/140‑1 (MQ=255)
                                  gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAATGTTTTTCACGGTTTTGTCACTGTACAAAAATGAt                                                                                                         >  2:2909004/1‑140 (MQ=255)
                                  gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTGCTCACATGGTTTTTCACGGTTTTAACACTGATAAACAATGAt                                                                                                         >  2:3922919/1‑140 (MQ=255)
                                  gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACAAGGTTTTTAAAGGTTTTATAACAGATAAAAAATGAt                                                                                                         >  1:4230144/1‑140 (MQ=255)
                                  gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACAATGTTTTTAACGGTTTTGTCACTGATaaaaaaatat                                                                                                         >  1:4377872/1‑136 (MQ=255)
                                  gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                                         >  2:2138342/1‑140 (MQ=255)
                                   tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                        <  2:2000393/140‑1 (MQ=255)
                                   tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                        <  2:2256100/140‑1 (MQ=255)
                                                 aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTAAACAAAACCGCgg                                                                                          >  2:1307418/1‑140 (MQ=255)
                                                 aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTTCTCACATGGTTTTTCAAGGTTTTGTCACTGATAAACAAAGATTAACCAAAAACGCgg                                                                                          >  1:3057792/1‑140 (MQ=255)
                                                 aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCGCACATGGTTTTTCACCGTTTTCTCAAGGATAAAAAAAGATTAACCAATAACGCgg                                                                                          >  1:4278297/1‑140 (MQ=255)
                                                 aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAACGGTTTTATCACTTATAAACAAAGATTAACCAAAACCGCgg                                                                                          >  2:845162/1‑140 (MQ=255)
                                                 aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAGATACAATTTTTTCAAAATAATTGTCACATGGTTTTTAACGGTTTTGTGACAGATAAACAATGCTTCACCCATACAGCgg                                                                                          >  1:3170717/1‑140 (MQ=255)
                                                 aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCATTTTTTGCAAAATATTTCTCACATGGTTTGTGACCGTTTTCTCACAGGAAAAAAAGGATTCACCACAACCCCgg                                                                                          >  1:2061338/1‑140 (MQ=255)
                                                  tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTAACCAATTCCGCGGt                                                                                         >  2:3508379/1‑140 (MQ=255)
                                                  tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTGATTTTTTTCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATCACTGATAAACAATGATTCACCAATAACGCGGt                                                                                         >  1:1640281/1‑140 (MQ=255)
                                                    tcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCTCACATTGTTTTTCACGGTTTTATAACTTATAAACAATGATTAAACAATAACGCGGTTg                                                                                       >  2:1186915/1‑140 (MQ=255)
                                                          cTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCttttt                                                                                 <  2:4509335/140‑1 (MQ=255)
                                                            aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                               <  1:1400502/140‑1 (MQ=255)
                                                            aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAAGGTTTTTCACGGTTTTCTAACTGATAAACAAAGATTAACCAAAAACGCGGTTGCtttttac                                                                               >  2:783555/1‑138 (MQ=255)
                                                                aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAAAATTGCTCACAAGGTTTTTAACGGTTTTGTCACTGATAAAAAATGATTGAACAAAACCGCGGGTGCGTTTTCCGTCt                                                                           >  2:4004599/1‑140 (MQ=255)
                                                                  ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTGATTTTTTTGAAAATATTTCTGCCATTGTTTTTTACGGGTTTATGACTGATAAAAAAAGATTCAACAATAACGCGGTTGGTTTTTACGTGTGc                                                                         >  1:494777/1‑140 (MQ=255)
                                                                  ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTGCAAAAAAATGCGCACATTGTTTTTCACGGTTTTATCCATGATAAACAATGATTCAACAAAACCGCGGTTGCCTTTTACGTCTGc                                                                         >  2:4781382/1‑140 (MQ=255)
                                                                   cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                        <  1:2655751/140‑1 (MQ=255)
                                                                      ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                     <  1:5084119/140‑1 (MQ=255)
                                                                      ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                     <  1:285732/140‑1 (MQ=255)
                                                                      ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                                     <  1:130419/140‑1 (MQ=255)
                                                                      ttctAACGGCGACCACAACGGCGTGCGTCCGGACGTTTACATTCATGTATTGCAAAATATTGCTCACAACCTTTTTCACGGTTTTCTCACTGATAAACAATGAGTCACCACGACCGCGGGTGCGTTTTCCGTCTGCAAGc                                                                     <  1:2552439/137‑1 (MQ=255)
                                                                            tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCGCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                                               >  2:3161404/1‑140 (MQ=255)
                                                                              gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                             <  1:1186915/140‑1 (MQ=255)
                                                                              gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTGTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCCCCTATACCGCGGTTGCTTTTTCCGTCGGCAAGCATCTgcag                                                             <  1:984494/140‑1 (MQ=255)
                                                                               cGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTTCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATCACTGATAAAAAATTATTCAACAAAACCGCGGTTGCTTTTTACGTCTGGAAGCAACagcagc                                                            >  1:3595857/1‑140 (MQ=255)
                                                                                             tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                              <  2:2557235/140‑1 (MQ=255)
                                                                                             tGCGTCCGGTCGTTTACAATCATTTTTTGCAAAAAATTGCTCACAATGTTTTTCACGGTTTTGTAACTTATAAACAAAGATTAACCAAAAACCCGGTTGCTTTTTCCCTATGAAATAATAAGCAGCAATTCACAttaacg                                              >  2:4380341/1‑136 (MQ=255)
                                                                                               cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                            <  1:2925125/140‑1 (MQ=255)
                                                                                                gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgccg                                           <  1:2794692/140‑1 (MQ=255)
                                                                                                 tCCGGTCGTTTACATTCATTTTTTGCAAAATATGGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGa                                          <  1:2185177/140‑1 (MQ=255)
                                                                                                       cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                    <  2:2414082/140‑1 (MQ=255)
                                                                                                       cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                    <  1:3429806/140‑1 (MQ=255)
                                                                                                       cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                    <  1:4113878/140‑1 (MQ=255)
                                                                                                       cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                    <  1:1721217/140‑1 (MQ=255)
                                                                                                       cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTaaa                                    <  1:3740330/140‑1 (MQ=255)
                                                                                                         tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                  <  2:277501/140‑1 (MQ=255)
                                                                                                         tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                  <  1:2121601/140‑1 (MQ=255)
                                                                                                                  attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAGGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                         >  2:1122651/1‑140 (MQ=255)
                                                                                                                  attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                         >  2:209742/1‑140 (MQ=255)
                                                                                                                  attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                         >  1:1583311/1‑140 (MQ=255)
                                                                                                                   ttttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGt                        <  2:4141443/140‑1 (MQ=255)
                                                                                                                   ttttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGt                        <  1:384690/140‑1 (MQ=255)
                                                                                                                    tttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTc                       >  2:1753252/1‑140 (MQ=255)
                                                                                                                      tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                     <  1:3520441/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACCTGGATTTTCACGGTGTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  1:349597/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  2:931996/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  1:2283754/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  1:4038894/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  1:4458136/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  2:2646548/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  2:1651529/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  2:4230144/140‑1 (MQ=255)
                                                                                                                        tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  1:519478/140‑1 (MQ=255)
                                                                                                                             attatTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTTCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt              <  1:4002404/138‑1 (MQ=255)
                                                                                                                             aaTATTGCTCACATGGTTTTTCACGGTTTTCTCCCTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt              <  2:3321950/140‑1 (MQ=255)
                                                                                                                             aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt              <  1:1753252/140‑1 (MQ=255)
                                                                                                                             aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt              <  1:845162/140‑1 (MQ=255)
                                                                                                                             aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt              <  1:4840767/140‑1 (MQ=255)
                                                                                                                             aaTATTGCTCACATGGTATTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt              <  2:1527914/140‑1 (MQ=255)
                                                                                                                             aaTAGTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt              <  2:5026016/140‑1 (MQ=255)
                                                                                                                                   gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt        <  1:456788/140‑1 (MQ=255)
                                                                                                                                   gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt        <  2:2127312/140‑1 (MQ=255)
                                                                                                                                   gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt        <  1:483583/140‑1 (MQ=255)
                                                                                                                                   gCTCACATGGCTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt        <  1:1087260/140‑1 (MQ=255)
                                                                                                                                     tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg      <  2:4644360/140‑1 (MQ=255)
                                                                                                                                     tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg      <  2:3059886/140‑1 (MQ=255)
                                                                                                                                     tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg      <  2:2346284/140‑1 (MQ=255)
                                                                                                                                       acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc    <  1:780800/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                   <  1:4739738/123‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  <  2:184819/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  <  1:676240/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  <  1:3358121/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  >  1:3237883/1‑140 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  <  1:1955821/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  <  1:1161681/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCAGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTATGCGGATTTCAGGATAGACCt  <  1:2436685/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  <  2:1928096/140‑1 (MQ=255)
                                                                                                                                         aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGAGTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt  <  1:636198/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCT  >  NZ_CP034943/3446849‑3447125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: