Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,995 C→A 8.1% V197V (GTG→GTT degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9950CA 8.1% 1054.2 / 7.5 412V197V (GTG→GTTdegUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base C (92/250);  new base A (53/2);  total (160/252)
Fisher's exact test for biased strand distribution p-value = 3.64e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 413 total aligned reads displayed.

AAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGA  >  NZ_CP034943/3446856‑3447133
                                                                                                                                           |                                                                                                                                          
aaaaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTAGCTCATTTCTACCCAGCCATTTTTAATGGCGACCACCACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTc                                                                                                                                            <  1:4227901/140‑1 (MQ=255)
  aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                          <  1:2488975/140‑1 (MQ=255)
  aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                                          <  1:1886732/140‑1 (MQ=255)
      gACCTGCCCAGTAAAAGGCAAGTCTCTTCTTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTGAAAATATTGGTCACATGGTTTTTCAAGGtt                                                                                                                                      >  2:3204440/1‑140 (MQ=255)
      gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGtt                                                                                                                                      >  2:1125995/1‑140 (MQ=255)
       aCCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGttt                                                                                                                                     >  2:780800/1‑140 (MQ=255)
          tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                                  <  1:1043739/140‑1 (MQ=255)
          tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                                  <  1:2391649/140‑1 (MQ=255)
          tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATATCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCCCGGTTTtct                                                                                                                                  <  2:179017/140‑1 (MQ=255)
          ggCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                                  <  1:364141/139‑1 (MQ=255)
          cgCCCACTAACAGGCAAGTCTCTTATTATACTATCTCACTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                                                  <  1:2925313/139‑1 (MQ=255)
            cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACCTTCATTTTTTTCAAAATATTGCGCCCAAGGGTTTTAACGGTTTTGTCa                                                                                                                                >  2:2111701/1‑140 (MQ=255)
            cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTAAAATGGTTTTTCACGGttttataa                                                                                                                                >  2:171812/1‑136 (MQ=255)
                 tAAAAGGCACGTCTCTGATTATACGCTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGGGCATTCATTTTTTGCCCAATATTGCTCACCTGGTGTTTCACGGGTTTCGGACTGAt                                                                                                                           >  2:4678501/1‑140 (MQ=255)
                 tAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGAt                                                                                                                           <  1:1457765/140‑1 (MQ=255)
                  aaaaGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGAGTCAGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATa                                                                                                                          <  1:257297/140‑1 (MQ=255)
                      ggCAAGTCTCTTATTATACTATCTCATGTCTACCCAGCCATTTTTAATGTCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTTCTCACATTGTTTTTAACGGTTTTGTAACATATaaaaa                                                                                                                      >  2:1246586/1‑138 (MQ=255)
                         aaGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTGCGGTCGTTTACATTCATTTTTTGCAAAAGGGTTCTCACATGGGTGTTGACGGTTTTCTCACTGGTATACAATg                                                                                                                   >  2:3627574/1‑140 (MQ=255)
                          aGTCTCTTATTATACTATCTCCTTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  2:1001187/140‑1 (MQ=255)
                          aGTCACTTATTATACTATCTCAGTTCTTCCCAGCCATTTTGAATGGCGACCCCAACCGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                                  <  1:1210006/140‑1 (MQ=255)
                           gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAAAATTTCTCACATGGTTTTTGACGGTTTTCTAACTTATAAACAATGAt                                                                                                                 >  2:450367/1‑140 (MQ=255)
                            tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                                <  2:2256100/140‑1 (MQ=255)
                            tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                                <  2:2141630/140‑1 (MQ=255)
                            tctcTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAtt                                                                                                                <  1:2704169/140‑1 (MQ=255)
                               ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAc                                                                                                             <  1:4954780/140‑1 (MQ=255)
                               ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAc                                                                                                             <  1:3234459/140‑1 (MQ=255)
                                ttattaTACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAcc                                                                                                            <  2:4116672/140‑1 (MQ=255)
                                 tattatACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTGGAAAAAATTTCTCACAAGGTTTTTAACGGTTTTCTCACTGATAAACAAAGATTCACCa                                                                                                           >  1:5088704/1‑140 (MQ=255)
                                      taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACAAGGTTTTTCACCGTTTTCTCACTGATAAAAAAAGATTCAACAATAcc                                                                                                      >  2:2744039/1‑140 (MQ=255)
                                      taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACCGTTTTCTCACTGATAAAAAATGATTAAACAATAcc                                                                                                      >  2:4243098/1‑140 (MQ=255)
                                      taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAATTTCTAACATGGTTTTTCACCGTTTTATAACTTATAAAAAATGATTCACAAATaac                                                                                                      >  2:636198/1‑138 (MQ=255)
                                          aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTTCAAAATATTGCTCACAAGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTCACCAAAACCCCgg                                                                                                  >  2:3352686/1‑140 (MQ=255)
                                          aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTATCACTGATAAACAATTATTCACCAATAACGCgg                                                                                                  >  2:1570709/1‑140 (MQ=255)
                                          aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAAATTTTTGCAAAAAATTGCTCACATGGTTTTTCACGGTTTTCGCAATTAAAAACAATTATTCAACAAAACCACgg                                                                                                  >  2:2128597/1‑140 (MQ=255)
                                          aTCTCATTTCTACCAAGCCATTTTTAATTGCGACCACAACGGCTTGCGTCCGGTCGTTTACAATCAATTTTTGCAAAATAATTCTCACAATGTTTTTAAAGGTTTTATAAATTATAAAAAATTATTAAACAATAACTCgg                                                                                                  >  2:4202787/1‑140 (MQ=255)
                                           tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACCGTTTTCTCACTGATAAAAAAAGATTAAACAATAACGCGGt                                                                                                 >  2:483509/1‑140 (MQ=255)
                                           tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTGAAAAAATTTCTAACAAGGTTTTTAACCGTTTTATCACTTATAAACAAATATTAACCAATACCCCGGt                                                                                                 >  1:2161729/1‑140 (MQ=255)
                                           tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTGATTTTTTTCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATCACTGATAAACAATGATTCACCAATAACGCGGt                                                                                                 >  1:1640281/1‑140 (MQ=255)
                                             tcATTTCTACCCAGCCCTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTg                                                                                               <  2:4749645/140‑1 (MQ=255)
                                                     aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                                       <  1:4012896/140‑1 (MQ=255)
                                                     aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                                       <  1:1400502/140‑1 (MQ=255)
                                                     aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAAATTCTCACAAGGTTTTTAACGGTTTTATCACTGATAAAAAATGATTCACCAATACCGCCGTTGCtttttac                                                                                       >  2:3583733/1‑138 (MQ=255)
                                                      cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCGCACATTGTTTTTCACGGGTTTGTCACTGATAAACAATGATTCACCAATAACCCGGTTGCTTTTTGCg                                                                                      >  1:3593985/1‑140 (MQ=255)
                                                             aTTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCaa                                                                               <  2:28240/140‑1 (MQ=255)
                                                               ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                             <  1:279606/140‑1 (MQ=255)
                                                               ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                             <  1:3492630/140‑1 (MQ=255)
                                                               ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                                             <  1:130419/140‑1 (MQ=255)
                                                                     tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCGCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                                                       >  2:3161404/1‑140 (MQ=255)
                                                                       gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:4448473/140‑1 (MQ=255)
                                                                       gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:1186915/140‑1 (MQ=255)
                                                                       gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCCTTTTCCGTCTGCAAGCATCTgcag                                                                     <  1:3505933/140‑1 (MQ=255)
                                                                        ggACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                                                    <  1:3382861/139‑1 (MQ=255)
                                                                              caACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                                              <  1:3049674/140‑1 (MQ=255)
                                                                                      tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                                                      <  2:4886862/140‑1 (MQ=255)
                                                                                      tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAACAATAACGCGGTTGCGTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTAccg                                                      >  2:4051268/1‑140 (MQ=255)
                                                                                        cGTCCGGTCGTTTACCTTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                                    <  2:919666/140‑1 (MQ=255)
                                                                                        cGTCCGGTAGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                                    <  2:3943030/140‑1 (MQ=255)
                                                                                         gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATGATTCACCAAAAACGCGGGTTCTTTTTCCGTCTGCAAGCATCTGCAGCACATAACATTAccgccc                                                   >  2:292463/1‑139 (MQ=255)
                                                                                             ggTCGTTTACATTCATTTTTTGCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATCACTGATAAAAAAAGATTCACCAATACCGCCGTTGCTTTTTCCGTCTGCAAGCATCTTCAACACGTCACATTCCCGCCGAGtt                                               >  2:2010707/1‑140 (MQ=255)
                                                                                             ggTCGTTTACATTAATTTTTTGGAAAATATTGCTCACATGGTTTTTCACGGGTGTGTGAGTGGTAAACAATGGTTCACCAATACAGCGGTTGGTTGTTGCGTGTGGCAGCTTCTGCTGGCCTTCACAGTGGCGCGGAGtg                                               >  2:5151174/1‑139 (MQ=255)
                                                                                              gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTGTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                              <  2:4551847/140‑1 (MQ=255)
                                                                                              gTCGTGTACATTCATTTTTTGCAAAAAATTGCTCACAAGGTTTTTGACGGTTTTGTCACTTATAAACAATGATTAACCAAAACCCCGGTTTCTTTTTCCGTCTGCAAGCCTCTGCAGCACTTCACAATCCCGCCGAGTTa                                              >  2:542852/1‑140 (MQ=255)
                                                                                                cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  2:2414082/140‑1 (MQ=255)
                                                                                                cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:656910/140‑1 (MQ=255)
                                                                                                cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:3161404/140‑1 (MQ=255)
                                                                                                cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:3819839/140‑1 (MQ=255)
                                                                                                cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  2:1356024/140‑1 (MQ=255)
                                                                                                cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:1721217/140‑1 (MQ=255)
                                                                                                cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                            <  1:2120150/140‑1 (MQ=255)
                                                                                                  tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                          <  1:4374730/140‑1 (MQ=255)
                                                                                                  tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAAACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                          <  2:1877690/140‑1 (MQ=255)
                                                                                                       attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa                                     <  2:2565780/140‑1 (MQ=255)
                                                                                                           attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                                 >  1:1583311/1‑140 (MQ=255)
                                                                                                            ttttttGTAAAATATTGCTGACATGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCTAGTTAAAATATGTAATGGt                                <  1:2004834/140‑1 (MQ=255)
                                                                                                                 tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:3307382/140‑1 (MQ=255)
                                                                                                                      aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:4095598/140‑1 (MQ=255)
                                                                                                                      aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:1753252/140‑1 (MQ=255)
                                                                                                                      aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:845162/140‑1 (MQ=255)
                                                                                                                      aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                      <  1:4840767/140‑1 (MQ=255)
                                                                                                                            gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  1:456788/140‑1 (MQ=255)
                                                                                                                            gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  2:1951137/140‑1 (MQ=255)
                                                                                                                            gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  1:28826/140‑1 (MQ=255)
                                                                                                                            gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  2:4969953/140‑1 (MQ=255)
                                                                                                                            gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  1:483583/140‑1 (MQ=255)
                                                                                                                            gCTCACATGGCTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                <  1:1087260/140‑1 (MQ=255)
                                                                                                                              tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg              <  2:3439639/140‑1 (MQ=255)
                                                                                                                              tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg              <  2:3059886/140‑1 (MQ=255)
                                                                                                                              tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg              <  2:2346284/140‑1 (MQ=255)
                                                                                                                                acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc            <  1:780800/140‑1 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                           <  1:4739738/123‑1 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:3912203/1‑140 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:2766436/140‑1 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  1:529221/1‑140 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:1955821/140‑1 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  2:3860078/140‑1 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:4243098/140‑1 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:1161681/140‑1 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          >  2:843261/1‑140 (MQ=255)
                                                                                                                                  aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt          <  1:2223082/140‑1 (MQ=255)
                                                                                                                                         ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg   <  1:724364/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGA  >  NZ_CP034943/3446856‑3447133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: