Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,447,038 T→G 10.7% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,447,0380TG10.7% 675.0 / 9.0 365N183T (AAC→ACC) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (117/189);  new base G (9/50);  total (126/239)
Fisher's exact test for biased strand distribution p-value = 5.17e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176
                                                                                                                                           |                                                                                                                                          
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAAAATTGCTCACAAGGTTTTTAACGGTTTTATAACTGTTAAAAAATTATTCACAAAAAACGCGGt                                                                                                                                            >  2:3997255/1‑140 (MQ=255)
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTCATTTTTTTGAAAAAATTTCTAACAAGGTTTTTAACCGTTTTATCACTTATAAACAAATATTAACCAATACCCCGGt                                                                                                                                            >  1:2161729/1‑140 (MQ=255)
     tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAAAATTTCGCACATGGTTTTTAAACGTTTTATCACTTATAAACAATGATTAACCAAAAACGCCGTTGCtt                                                                                                                                       >  1:4791314/1‑140 (MQ=255)
          aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                                                                                  <  1:4012896/140‑1 (MQ=255)
          aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAAAGGTTTTCTCACTGATAAACAATTATTAACAAATACCGCGGTTGCtttttac                                                                                                                                  >  2:4529751/1‑138 (MQ=255)
           cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTGATTTTTTTGAAAATATTGCGCACAAGGTTTTTAACGGTTTTGTCACTGATAAACAAAGATTAAACAAAAACGCGGGTTCTTTTTGCg                                                                                                                                 >  1:4644360/1‑140 (MQ=255)
           cccAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAAAATTGCGCACATTGTTTTTCACGGGTTTGTCACTGATAAACAATGATTCACCAATAACCCGGTTGCTTTTTGCg                                                                                                                                 >  1:3593985/1‑140 (MQ=255)
                ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTGCAAAAAAATGCGCACATTGTTTTTCACGGTTTTATCCATGATAAACAATGATTCAACAAAACCGCGGTTGCCTTTTACGTCTGc                                                                                                                            >  2:4781382/1‑140 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  1:3492630/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  1:279606/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  1:2679082/140‑1 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTAACCAAAACCGCGGTTGCTTTTTGCGTGTGCAAGCAACTgc                                                                                                                  >  2:1940291/1‑140 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATTATTAACCAATAACGCGGTGTCGTTTTCCGTCTGCCAGCATCttc                                                                                                                  >  2:519268/1‑138 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                                                                                                  <  1:4837361/140‑1 (MQ=255)
                          tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAACCGTTTTCTCACTGATAAACAAAGATTCACCCATACCGCCGGTGCTTTTTCCGTCTGCAACCATCTgc                                                                                                                  >  2:2120150/1‑140 (MQ=255)
                            gCGACCACCACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  2:5066626/140‑1 (MQ=255)
                             ggACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagc                                                                                                               <  1:3382861/139‑1 (MQ=255)
                              gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                                                                              <  2:2772714/140‑1 (MQ=255)
                                   caccGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAAGACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                                                                                         <  2:2996361/137‑1 (MQ=255)
                                   caACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                                                                                         <  1:3049674/140‑1 (MQ=255)
                                      cGGCTTGCGTCCGGTCGTTTACATTGATTTTTTTGAAAAAATTTCTCACAATGTTTTTAAAGGTTTTATAACTGATAAAAAAATATTCACCAAAAACCCCGTTGCTTTTTTCGTCTGCAAGCAAATGCAGCACATaacaa                                                                                                      >  1:1618097/1‑139 (MQ=255)
                                           tGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCAACAATAACGCGGTTGCGTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTAccg                                                                                                 >  2:4051268/1‑140 (MQ=255)
                                           tGCGTCCGGTCGTTTACAATCATTTTTTGCAAAAAATTGCTCACAATGTTTTTCACGGTTTTGTAACTTATAAACAAAGATTAACCAAAAACCCGGTTGCTTTTTCCCTATGAAATAATAAGCAGCAATTCACAttaacg                                                                                                 >  2:4380341/1‑136 (MQ=255)
                                             cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                                                                               <  1:4233239/140‑1 (MQ=255)
                                             cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                                                                               <  1:3718575/140‑1 (MQ=255)
                                             cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                                                                               <  1:2925125/140‑1 (MQ=255)
                                             cGTCCGGTAGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAATGATTGACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCTCATTCccgcc                                                                                               <  1:3509532/140‑1 (MQ=255)
                                              gTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgccg                                                                                              <  2:585206/140‑1 (MQ=255)
                                                  ggTCGTTTACATTCATTTTTTGCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATCACTGATAAAAAAAGATTCACCAATACCGCCGTTGCTTTTTCCGTCTGCAAGCATCTTCAACACGTCACATTCCCGCCGAGtt                                                                                          >  2:2010707/1‑140 (MQ=255)
                                                   gTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                                                                         <  2:2423904/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACCTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:1737688/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:494777/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:4113878/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:4380341/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:2120150/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:1145215/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:1356024/140‑1 (MQ=255)
                                                       tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     <  1:1354801/140‑1 (MQ=255)
                                                       tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAat                                                                                     <  1:861861/140‑1 (MQ=255)
                                                       tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGCTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                                                                                     <  1:3834257/140‑1 (MQ=255)
                                                                attTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATgg                                                                            >  2:2634087/1‑140 (MQ=255)
                                                                 ttttttGTAAAATATTGCTGACATGGTTTTTAACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCTAGTTAAAATATGTAATGGt                                                                           <  1:2004834/140‑1 (MQ=255)
                                                                 ttttttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGt                                                                           <  2:4141443/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:4458136/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  2:1829655/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  1:1190490/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:519478/140‑1 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCCCTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  2:3321950/140‑1 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  1:4095598/140‑1 (MQ=255)
                                                                             tatTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCa                                                               <  1:1125995/140‑1 (MQ=255)
                                                                               ttGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAgg                                                             <  1:1935895/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:5043634/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:1274918/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:28826/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  2:16054/140‑1 (MQ=255)
                                                                                 gCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  2:3266897/140‑1 (MQ=255)
                                                                                  cTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATa                                                          >  1:2355229/1‑140 (MQ=255)
                                                                                   tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg                                                         <  2:3439639/140‑1 (MQ=255)
                                                                                   tCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAg                                                         <  2:2346284/140‑1 (MQ=255)
                                                                                     acaTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc                                                       <  1:780800/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:3860078/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:4529751/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  2:1905663/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:3912203/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:1083733/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:2551345/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:2766436/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:3237883/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:4849364/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:676240/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATGCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1683055/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGAGTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:636198/140‑1 (MQ=255)
                                                                                          gTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCAt                                                  >  2:4611292/1‑140 (MQ=255)
                                                                                               tCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       >  2:1517189/1‑98 (MQ=255)
                                                                                               tCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:1517189/98‑1 (MQ=255)
                                                                                               tCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  2:2148259/1‑132 (MQ=255)
                                                                                                  cGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  1:489562/140‑1 (MQ=255)
                                                                                                    gTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGgtg                                        <  1:1015576/140‑1 (MQ=255)
                                                                                                          tcACTGATAAACAATGATTCACCAAGACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGa                                  <  1:3352686/140‑1 (MQ=255)
                                                                                                               gATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTc                             >  2:3613009/1‑140 (MQ=255)
                                                                                                               gATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTc                             >  2:903225/1‑140 (MQ=255)
                                                                                                                 tAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTGTGCAAGAATCTGCAGCAGTTCACTTTCCCGCCGAGTTAAAAGATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCg                           >  2:2280023/1‑140 (MQ=255)
                                                                                                                   aaCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCt                         <  2:731161/140‑1 (MQ=255)
                                                                                                                   aaCAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCt                         <  2:1206465/140‑1 (MQ=255)
                                                                                                                      aaTGATTCACCAATAACGCGTTTTCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCAAATTCCAGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGTGGGTGTGCAGAAACTCCTCttgt                      >  2:3062754/1‑140 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  2:4694269/1‑140 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  1:5089307/1‑140 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  2:317643/1‑140 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  2:1692465/1‑140 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  1:551510/1‑140 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATGCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAGAGACCTCATGCTGAGGGTTTGCAGAAACTCCGCttgttg                    >  2:919950/1‑140 (MQ=255)
                                                                                                                          aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  1:198245/1‑140 (MQ=255)
                                                                                                                                cAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggcgg            <  1:3294039/140‑1 (MQ=255)
                                                                                                                                  aTACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGgcggcg          >  2:2536052/1‑140 (MQ=255)
                                                                                                                                   tACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGcggcgg         >  1:2197352/1‑140 (MQ=255)
                                                                                                                                      cgcgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGAc                                                       >  1:4847583/1‑91 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAAtac    >  2:3534226/1‑138 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  1:2824195/1‑140 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGt  >  1:3164838/1‑140 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGt  >  1:1807393/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: