Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,496,440 G→T 6.6% Q330K (CAA→AAA)  EO946_RS17995 ← glucosaminidase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,496,4400GT 6.6% 872.4 / 5.8 318Q330K (CAA→AAA) EO946_RS17995glucosaminidase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (63/221);  new base T (32/2);  total (95/223)
Fisher's exact test for biased strand distribution p-value = 7.59e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

TGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAATAA  >  NZ_CP034943/3496306‑3496579
                                                                                                                                       |                                                                                                                                           
tGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGGCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGTTGGTGGTTGACGGGTAATGGGCTTTTTTGTTTTTAAATCAATTGTGTATGTTTTGGAgcg                                                                                                                                        >  1:2910185/1‑139 (MQ=255)
  cGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTTATTCTGGATTACGTGTAATTCCATTTTTTATTTTTAAAGTAATTGTGTATCTTTTTCAgctgg                                                                                                                                      >  2:3945319/1‑139 (MQ=255)
    gCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGctgctg                                                                                                                                    <  1:3034310/140‑1 (MQ=255)
      tttgtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGtt                                                                                                                                  <  1:2270582/140‑1 (MQ=255)
         gtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCg                                                                                                                               <  1:985654/140‑1 (MQ=255)
         gtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCg                                                                                                                               <  1:2704121/140‑1 (MQ=255)
         gtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCg                                                                                                                               <  1:1665758/140‑1 (MQ=255)
         gtttgCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCg                                                                                                                               <  1:2172738/140‑1 (MQ=255)
           ttgcttgcTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGtt                                                                                                                             <  1:2223498/140‑1 (MQ=255)
             gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                                                                                           <  1:1140747/140‑1 (MQ=255)
             gcttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTaa                                                                                                                           <  1:760955/140‑1 (MQ=255)
              cttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTGGTTTTTTTGATGGTGGATGAAGTGTAAAGCCATTTTTTGTTTTTAAAGAAAGTTTCGTACGTGTGCAGCTGGTGTTTCggttac                                                                                                                          >  2:2787856/1‑135 (MQ=255)
               ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATGGGCTGTAGACATGGTCGTTTTTTTTATGCTTGATGCCGTGTAATGGCATTTTTTCTTTTTAAATAAAGTGTCTATGTTTTTCAGCTGCTGTTTCGTTaaac                                                                                                                         >  2:4523666/1‑138 (MQ=255)
               ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCGATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                                                                                         <  2:1563864/140‑1 (MQ=255)
               ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                                                                                         <  1:3376318/140‑1 (MQ=255)
               ttgctGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                                                                                         <  2:3777186/140‑1 (MQ=255)
               gtgctgcTCGCCTAACATCGGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCGGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcc                                                                                                                         <  2:1348118/139‑1 (MQ=255)
                 gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACCTGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                                                                       <  2:2972736/140‑1 (MQ=255)
                 gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                                                                       <  1:1497111/140‑1 (MQ=255)
                 gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                                                                       <  1:1301503/140‑1 (MQ=255)
                 gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                                                                       <  2:3676361/140‑1 (MQ=255)
                 gctgctCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAAcccc                                                                                                                       <  2:1710710/140‑1 (MQ=255)
                      tCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCat                                                                                                                  <  1:2327741/140‑1 (MQ=255)
                         ccTAAAATCTGCGGCGTCGTTATTGGATTGGCTGTAGACCTGGTCGTTTTTTTGAGGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCGATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                               <  2:146253/140‑1 (MQ=255)
                         ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                               <  1:3210551/140‑1 (MQ=255)
                         ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                               <  1:4534443/140‑1 (MQ=255)
                         ccTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                               <  2:2880661/140‑1 (MQ=255)
                         ccTAAAATCTGCGCCGTAGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAtt                                                                                                               <  2:2039339/140‑1 (MQ=255)
                          cTAAATTCTGCGGCGTCGTTATTGGATAGGCTGTAGAGATTGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTATAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATAGTc                                                                                                              <  1:5170898/140‑1 (MQ=255)
                          cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                                                                              <  1:4146921/140‑1 (MQ=255)
                          cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                                                                              <  1:5127008/140‑1 (MQ=255)
                          cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                                                                              <  1:237914/140‑1 (MQ=255)
                          cTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTc                                                                                                              <  1:2108509/140‑1 (MQ=255)
                           tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                             <  1:1607129/140‑1 (MQ=255)
                           tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                             <  2:3058829/140‑1 (MQ=255)
                           tAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCa                                                                                                             <  2:4282309/140‑1 (MQ=255)
                            aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                            <  1:864140/140‑1 (MQ=255)
                            aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                            <  1:4380910/140‑1 (MQ=255)
                            aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                            <  2:3641781/140‑1 (MQ=255)
                            aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                            <  1:3268381/140‑1 (MQ=255)
                            aaaaTCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCAc                                                                                                            <  1:1072039/140‑1 (MQ=255)
                                 cTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAAGGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTGCGTTAACCCCTTCATATTCACTGtat                                                                                                       <  1:586258/140‑1 (MQ=255)
                                    cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATTCTGGGTGACGTGTAATGCCATTTTTTCTTTTTAAATAAAGTGTGTTTCGTTTTCAGCTGCTGTTTCGTTAAACCCTTCATATTCACTGTATatg                                                                                                    >  2:3896765/1‑140 (MQ=255)
                                    cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGTTGTCGTGTAATTCCATTTTTTGTTTTTAAAGAAAGTGTCTATCGTTTTAAGCTTCTGTTTAGTTAAAACCATAATATTGACTGTatatt                                                                                                    >  2:570005/1‑139 (MQ=255)
                                    cgcCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatg                                                                                                    <  1:1196127/140‑1 (MQ=255)
                                      ccGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATatgat                                                                                                  <  1:139854/140‑1 (MQ=255)
                                                  gagaGGCTGTACACATGCTCGTTTTTTTGATGCTGGATGACGTGTAATGCCCTTTTTTCTTTTGAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCa                                                                                      <  2:2911135/137‑1 (MQ=255)
                                                   aTAGGCTGTAGACATGGTCGTTTTTTTGATGAGGGATGACGTGTAATGCCATTTTTTATTTTTAAAGAAACAGTCTAAGTTTTGCAGCTGCTGTTTCGTTAAACCCATCAGAGTAACTGTGTATGATGTTGTTTTTTCaa                                                                                     >  2:4087013/1‑140 (MQ=255)
                                                    tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                                                                                    <  2:2253361/140‑1 (MQ=255)
                                                    tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                                                                                    <  2:1045988/140‑1 (MQ=255)
                                                    tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                                                                                    <  1:3995961/140‑1 (MQ=255)
                                                    tAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAAc                                                                                    <  1:2955445/140‑1 (MQ=255)
                                                       gCTGTAGACATGGTCGTTTTTTTTATTCTGGATGACGTGTAATGCCCTTTTTTTTTTTTAAAGAAAGTGTGTTTCTTTTGGATCTGCTGGTTCGTTAACCCCGTCAAATTAACTGTATATGATGTGCTTTTGTCAAACTg                                                                                 >  1:5216550/1‑140 (MQ=255)
                                                       gCTGTAGACATGGTCGTTTTTTTGATTCTGGATGACGGGTAATGCGATTTTTTCTTTTTAAAGAAAGTGTGTGTCTTTTGCAGCTGCTGTTTCGTTAACACCGTCAGATTGACTGTATATGATGTGGTTTGTTCCACCTg                                                                                 >  2:2955263/1‑140 (MQ=255)
                                                       gCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTTCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTTCTTTTTTCAACCtt                                                                                 >  2:4934906/1‑139 (MQ=255)
                                                       gCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGGGTAATTGCCATTTTTCTTTTTAAATAAAGTGTGTATCTTTTTCAGCTGATGTTTCGTTAAACCCATCATATTAACTGTATATGATGTGGTTTTTTCAAACtt                                                                                 >  2:1171364/1‑139 (MQ=255)
                                                       gCTGTAGACATGGTCGTTTTTTTGAATCTGGAAGACGTGTAAATCCATTTTTTAATTTTAAAGAAAGTGTCTATCTTTTTCAGCTTCTTTTTAGTTAAACACTTCATATTGACTTTAAATGTTTTTCTGTTGTTAACCTg                                                                                 >  2:252029/1‑140 (MQ=255)
                                                         tGTAGACATGGTCGTTTTTTTTTTTGTGGATTACGTGTTATTCCATTTTTTGTTTTTAAAGAAAGTGTTTATGTTTTGCAGGTGGTGTTTTGTTAAACCCATCATATTAACAGTATATGATGTTCTTTTTTAAAACTGtt                                                                               >  1:3554180/1‑140 (MQ=255)
                                                         tGTAGACATGGTCGTTTTTTTTATTCTTGTTGACGTGTTATGCCCATTTTTATTTTTAAATAAAATGTGTATCTTTTTCAGCTGCTGTTTCGTTTAACCCATCATATTCACTGTATATTATTTGCTTTTTTGAAcacagt                                                                               >  1:3829611/1‑135 (MQ=255)
                                                         tGTAGACATGGTCGTTTTTTTTATTCGGGATGACGTGTAATGCCATTTTTTGTTTTTAAAGAAAGTTTGTATCTTTTTCAGCGGCTGTTTCGTTAACACCATAATATTCACTGTATATTATGTGGTTTTTTCAAACTGtt                                                                               >  2:2692197/1‑140 (MQ=255)
                                                           tAGACATGGTCGTTTTTTTTTTTGTGGATGACGTGTAATTGCATTTTTTCTTTTTAAAGAAAATGTCGATCTTTTTCAGCTGCTGTTTGGTTAACACCCTCATATTGACTGTATATGATTTTGTTTTTTAAACCTGTTGa                                                                             >  2:1957412/1‑140 (MQ=255)
                                                            aGACATGGTCGTTTTTTTGTTGCTGGATTTCGTGTAATGCCATTTTTTATTTTTAAATAAAGTGTCTATCTTTTGGAGCTGCTGTTTAGTTAAACCCATAATATTCAATGTATATTAAGTGCTTTTTTGAAACTTTTGAt                                                                            >  2:4679308/1‑140 (MQ=255)
                                                            aGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGAt                                                                            <  1:3932620/140‑1 (MQ=255)
                                                            aGACATGGTCGTTTTTGTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGAt                                                                            <  1:2833129/140‑1 (MQ=255)
                                                             gACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATa                                                                           <  2:4382066/140‑1 (MQ=255)
                                                              aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa                                                                          <  2:3444605/140‑1 (MQ=255)
                                                              aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa                                                                          <  2:2159070/140‑1 (MQ=255)
                                                              aCATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATaa                                                                          <  2:2505905/140‑1 (MQ=255)
                                                                aTGGTCGTTTTTTTGATGGTGGCTGACGGGTAAAGCCATTTTTTCTTTTTAAAGAAAGTGTGGATGTTTTGGGGCTGCTGTTTGGTTAACCCCGTAATAGTGACGGTATATGATGTTCTTTTTTGAACCTGTTGATAACt                                                                        >  2:823872/1‑140 (MQ=255)
                                                                aTGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAATAAACGGTCTATCTTTTGCAGCTGCTGTTTGGTTAAACCCTTCATATTCACTGTATATTTTGTGCTTTTGTCAACCGGTTGATAACt                                                                        >  2:3528985/1‑140 (MQ=255)
                                                                 tGGTCGTTTTTTTGATGCTGGATTACGGGTAATGCCATTTTTTCTTTTTAAAGAAATTGTCGATCTTTTGCACCTGCTGTTTCGTTAACCCCGTCATATTCACAGTATATTATGTTCTTTTTTATACAGGTTGATAACta                                                                       >  2:5178351/1‑139 (MQ=255)
                                                                  ggTCGTTTTTTTTATTGTGGATGACGTGTAATGCCATTTTTTATTTTTAAATAAAATGTGTATATTTTGCAGATGGTGTTTGGTTAAACCCTTAATATTAAGTGTATATGATGTGGGTTTGTGAAACTGTTTATTACTGc                                                                      >  2:2391844/1‑140 (MQ=255)
                                                                  ggTCGTTTTTTTTATGCTGGCTGACCTGTAATGCCATTTTTTGTTTTTAAACAAAGTGTATATCTTTTGCAGCTGCTTTTTATTTAAACCCATCATATTCACTGTATATGATGTGATTTTTTGAAACTTTTTATAAATGc                                                                      >  2:3745844/1‑140 (MQ=255)
                                                                  ggTCGTTTTTTTTATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGTAGCTGCTGTTTCGTTAACCCCATCATATTCAATTTATATTATGTGCTTTTGTAAACCTGTTGATaaaagc                                                                      >  2:2577425/1‑136 (MQ=255)
                                                                  ggTCGTTTTTTTGATTCTGGATGACGTGTAATTCCCTTTTTTCTTTTTAAATAAAGTGTCTATCGTTTGCAGCTGCTGTTTCGTTAAACCCATCATATTCACTGTATATGATGTTCTTTTGTCAACCATTTGATAACTGc                                                                      >  2:4903243/1‑140 (MQ=255)
                                                                  ggTCGTTTTTTTGATTCAGGATGAAGTGTAATGCCAATTTTTCTTTTTAAAGAAAGTGTATATATTTTGAAGCGGCTTTTTAGTTAAAACCATCAAATTCAAATTATATGATGTGCTTTTTTCAAAATTTTTATAAATGc                                                                      >  2:1602114/1‑140 (MQ=255)
                                                                  ggTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTGGTGTTAAAGAAAGTGGGTATCTTTTGCAGGTGCTGTTTCGTTAAACCCGTCATATTCACTGTATATGATGTGGGTTTGTCAACCTGTTGATAACTGc                                                                      >  2:3138243/1‑140 (MQ=255)
                                                                   gTCGTTTTTTTGATTCTGGATGACGTGTAAAGCCCTTTTTTGTTTTTAAAGAAAGTGTCTATCTTTTGCAGCAGCTGTTTAGTTAAACCCTTAATATTAACTGTATATGATGTGGATTTGTCATACTGTTGATAACAGcc                                                                     >  2:5053267/1‑140 (MQ=255)
                                                                   gTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGcc                                                                     <  1:312990/140‑1 (MQ=255)
                                                                              gATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCgg                                                          <  1:729593/140‑1 (MQ=255)
                                                                                       tGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATAGGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCt                                                 <  2:1085120/140‑1 (MQ=255)
                                                                                               aaTGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTgg                                         >  2:4531270/1‑140 (MQ=255)
                                                                                                aTGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGc                                        <  1:2556828/140‑1 (MQ=255)
                                                                                                     cATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGAt                                    >  1:4336536/1‑140 (MQ=255)
                                                                                                      aTTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGACTGATACGCCGGTGCTATTCTTGATTTGGCTGATg                                   <  2:747342/140‑1 (MQ=255)
                                                                                                        tttttCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATg                                   >  1:3612704/1‑138 (MQ=255)
                                                                                                             cTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCt                            <  1:2055069/140‑1 (MQ=255)
                                                                                                               ttttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTc                          >  1:4108407/1‑140 (MQ=255)
                                                                                                                tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                         <  1:630256/140‑1 (MQ=255)
                                                                                                                tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                         <  2:1831207/140‑1 (MQ=255)
                                                                                                                tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                         <  1:4236200/140‑1 (MQ=255)
                                                                                                                tttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCa                         <  2:2454388/140‑1 (MQ=255)
                                                                                                                 ttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCAc                        <  2:4129849/140‑1 (MQ=255)
                                                                                                                 ttAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCAc                        <  2:1254191/140‑1 (MQ=255)
                                                                                                                  tAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACt                       <  2:2809513/140‑1 (MQ=255)
                                                                                                                              cTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCt           <  1:2634896/140‑1 (MQ=255)
                                                                                                                              cTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCt           <  1:5086746/140‑1 (MQ=255)
                                                                                                                              cTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCt           <  1:4903243/140‑1 (MQ=255)
                                                                                                                                     ttGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAAt    >  1:2521589/1‑140 (MQ=255)
                                                                                                                                       gCAGCTGGTGTTTCGTTAACCGCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAAGTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAATaa  <  1:191150/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
TGCGGCTTTGTTTGCTTGCTGCTCGCCTAAAATCTGCGCCGTCGTTATTGGATAGGCTGTAGACATGGTCGTTTTTTTGATGCTGGATGACGTGTAATGCCATTTTTTCTTTTTAAAGAAAGTGTCTATCTTTTGCAGCTGCTGTTTCGTTAACCCCTTCATATTCACTGTATATGATGTGCTTTTGTCAACCTGTTGATAACTGCCTGATACGCCGGTGCTTTTCTTGATTTGGCTGATGGCGTCCTTCACTGTATTCAGCCCTTTGAAATAA  >  NZ_CP034943/3496306‑3496579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: