Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,559,345 T→G 7.2% N205H (AAC→CAC)  EO946_RS18295 ← SpaA isopeptide‑forming pilin‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,559,3450TG 7.2% 715.4 / 4.1 297N205H (AAC→CAC) EO946_RS18295SpaA isopeptide‑forming pilin‑related protein
Reads supporting (aligned to +/- strand):  ref base T (129/130);  new base G (36/2);  total (165/132)
Fisher's exact test for biased strand distribution p-value = 2.78e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

AATCATCACTCTTAGAAGAATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTAAACGTGGCT  >  NZ_CP034943/3559207‑3559484
                                                                                                                                          |                                                                                                                                           
     tcaCTCTTAGAAGAATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGGTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCgg                                                                                                                                       <  2:576253/140‑1 (MQ=255)
      caCTCTTAGAAGAATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGc                                                                                                                                      >  1:1003255/1‑140 (MQ=255)
          ctTAGAAGAATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGttttt                                                                                                                                          >  1:3804018/1‑132 (MQ=255)
             agaagaATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCTTCCg                                                                                                                               >  1:32802/1‑140 (MQ=255)
             agaagaATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTTGGGTCTTACg                                                                                                                               >  1:3040969/1‑140 (MQ=255)
             agaagaATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTGGGCGCTTGCg                                                                                                                               >  1:5123845/1‑140 (MQ=255)
               aagaaTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTGCGtg                                                                                                                             >  2:1908992/1‑139 (MQ=255)
               aagaaTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTGTTAGGCTCTTACGtc                                                                                                                             >  2:729845/1‑140 (MQ=255)
                agaaTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCTTCCGtct                                                                                                                            >  1:4257829/1‑140 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGTTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTAGGCTCTTACGtatagg                                                                                                                         >  2:4673370/1‑135 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGTGTCGTGTTTCGGCACTTCCGGCTCgg                                                                                                                         >  2:5200296/1‑140 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTCCGTCTCgg                                                                                                                         >  1:4948370/1‑140 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCgg                                                                                                                         >  2:2607317/1‑140 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTACGTGTCgg                                                                                                                         >  2:2737909/1‑140 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTACGTCTCgg                                                                                                                         >  1:3355536/1‑140 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTGGTACGTCTCgg                                                                                                                         >  2:223990/1‑140 (MQ=255)
                   aTCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCTTGCGTCTCgg                                                                                                                         >  2:4467527/1‑140 (MQ=255)
                    tCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTCCGTCTCGGc                                                                                                                        >  1:3443419/1‑140 (MQ=255)
                       ccACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGAGTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGCTCTTACGTCTGGGCCTc                                                                                                                     >  1:2946391/1‑140 (MQ=255)
                        caacgttAGTGTTAGCTGAGTCCTGGTGTTGTTTCTCTTTTTTGTCCTGGCTGTCTTAAGGTTCATATTTGTCATCTGTGCATTCCTGATCTCGGGCAGTTTCAGTATAGTGGTTTTTCGGCTCTTCCGTCTGGGAATCt                                                                                                                    <  2:2176354/135‑1 (MQ=255)
                        cACCCTTAGTGTCAGCTGATGCCTGTTGTACTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCt                                                                                                                    <  2:4583795/140‑1 (MQ=255)
                              tAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGCTCTTCCGTCTCGGCCTCTGGTGtc                                                                                                              >  1:4870374/1‑140 (MQ=255)
                               aGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCTTCCGGATCGGCCTCTGGTGtcg                                                                                                             >  1:2432048/1‑139 (MQ=255)
                               aGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTAGGGTCTTCCGTCTCGGCCACTGGGGtct                                                                                                             >  1:5205646/1‑140 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTTGGGTGTTACGTGTCGGCATCTGGTGTGTCAtatt                                                                                                       >  1:1554012/1‑140 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTCCGTCTCGGCATCTGGTGTATCaaatt                                                                                                       >  1:4483331/1‑136 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCtt                                                                                                                                  >  1:2373047/1‑113 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCtt                                                                                                                                  <  2:2373038/113‑1 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTGCGTCTCGGCCTCTGGTGGCTCAactt                                                                                                       >  2:1299445/1‑140 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCTTGCGTCTCGGCCTCTGGTGTCTCAtatt                                                                                                       >  1:902311/1‑140 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTGGGCGCGTGCGTCTCGGCCTCTTGTGTCTCAtctt                                                                                                       >  1:1811552/1‑140 (MQ=255)
                                     aGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCGCAtctt                                                                                                       >  2:316435/1‑140 (MQ=255)
                                        tGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCAtctttct                                                                                                    <  2:2625682/140‑1 (MQ=255)
                                        tGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCAtctttct                                                                                                    <  2:3724723/140‑1 (MQ=255)
                                          aTGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGCGCTTCCGTCTCGGCATCTGGTGTCTCATATTTGTTa                                                                                                  >  1:1421768/1‑140 (MQ=255)
                                           tGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTaa                                                                                                 <  1:4387257/140‑1 (MQ=255)
                                           tGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGGTCTTCCGTCTCGGCATCTGGTTTATCAtctttcttta                                                                                                 >  2:3687510/1‑138 (MQ=255)
                                           cgCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCACGGCATCTGGTGTCTCATCTTTCTTaa                                                                                                 >  2:3659940/2‑140 (MQ=255)
                                              cggttgttCCTCCTCGATTGGCTCCTTGCTGAATGAAGGTGCAGATTTTTGATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAg                                                                                              <  2:3009469/138‑1 (MQ=255)
                                              cTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAg                                                                                              <  2:4108365/140‑1 (MQ=255)
                                              cTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAg                                                                                              <  2:97032/140‑1 (MQ=255)
                                              cTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTAGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAg                                                                                              <  1:4673370/140‑1 (MQ=255)
                                                 ttgttCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGttt                                                                                                                                            <  2:3354885/91‑1 (MQ=255)
                                                 ttgttCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGGTCTTGCGTCTCGGCAGCTGGTGTCTCATCTTTCTTAACAGGga                                                                                           >  2:3791967/1‑140 (MQ=255)
                                                 ttgttCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGGTCTTACGTCTCGGCATCTGGTGTGTCATATTTATTAAAAGGga                                                                                           >  1:2675/1‑140 (MQ=255)
                                                 ttgttCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCGTCCGTCTCGGCATCGGGTGTCTCATATTTCTTAAAAGGga                                                                                           >  1:207765/1‑140 (MQ=255)
                                                 ttgttCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTATGGGCATACCTCTCGGCAACTGGTTTCTAAACTATATTAAAACGga                                                                                           >  1:10760/1‑140 (MQ=255)
                                                    ttcttcCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGgatga                                                                                        >  2:2012197/1‑140 (MQ=255)
                                                    ttcttcCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTATTTCGTGTCGGCATCTTGTGTCTCATATTTTTTTACAGGgattt                                                                                        >  2:5128872/1‑138 (MQ=255)
                                                      cttcCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTTGGGGCTTCCGTCTCGGCATCTTGTGTATCATATTTCTTAAAAGGgatgtta                                                                                      >  1:2864302/1‑137 (MQ=255)
                                                      cttcCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTACGTCTCGGCATCTGGTGTGTCATATTTATTTAAACGGCTGATc                                                                                      >  2:2131513/1‑140 (MQ=255)
                                                      cttcCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCGCTTGCGTGTCGGCATAAGGTGTCTCAACTTTATTTAAATGGATGATc                                                                                      >  1:994868/1‑140 (MQ=255)
                                                        tcCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCAc                                                                                    <  2:1886140/140‑1 (MQ=255)
                                                         ccTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTCCGTCTAGGCATCTGGTGTCACATCTTTGTTAAAAGGGATTATCaaa                                                                                   >  2:686234/1‑138 (MQ=255)
                                                           tctTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCTTACGGATAGGCATCTGGTGTCTCATCTTTATTAACAGGgatgataaacgg                                                                                 >  2:1719536/1‑134 (MQ=255)
                                                                  gCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGtt                                                                          <  1:2125767/140‑1 (MQ=255)
                                                                       ttGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTACGTCTCGGCAAATTGTGTGTCATCATTGTTAAAAGGGGTGATAACCAGCTGGGtttttgc                                                                     >  1:501014/1‑137 (MQ=255)
                                                                       ttGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGcc                                                                     <  1:2732069/140‑1 (MQ=255)
                                                                        tGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCt                                                                    <  1:2314317/140‑1 (MQ=255)
                                                                                    gTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCGGGTCCGTCGCGGCCGCTGGTGGGTGATATTTCGTAAAACGGATGATCACCGGCAGGGTTTTTGCTCCCCCGAGttta                                                        >  1:328639/1‑138 (MQ=255)
                                                                                      gCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCGCGTCCGTCTCGGCATCTGGTGGGTCATGTTTCTTTAAAGGGATGATCACCGGCTGGGGTTTGGCTCCCCCTAGTTGaaa                                                      >  1:4167438/1‑140 (MQ=255)
                                                                                       cAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTCCGTCTGGGCAGCTGGTGTGTCATGTTTGTTAACAGGGGTGATCACCAGCTGGGTTTTTGCTCCCCCAAGTTGtaaa                                                     >  1:2187832/1‑140 (MQ=255)
                                                                                       cAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGCGCGTGCGTCTCGGCATCTGGTGTCTCAAATTTGTTAAAAGGGATTATCACCAGCGTGGTTTTTACTACCCCTAATTTaaaa                                                     >  2:2492732/1‑140 (MQ=255)
                                                                                         taTTTGTCATCTGTCCCTTCCTGATCACCCGCAGTGTCATAAGAGTCGTGTTTCGGCTCTTCAGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAg                                                   <  2:4053625/139‑1 (MQ=255)
                                                                                         gttttGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGTGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAg                                                   <  2:5181985/138‑1 (MQ=255)
                                                                                           tttGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTg                                                                                                                   >  2:1140399/1‑74 (MQ=255)
                                                                                           tttGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAggg                                                 <  1:537430/140‑1 (MQ=255)
                                                                                           tttGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAggg                                                 <  1:1891224/140‑1 (MQ=255)
                                                                                            ttGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgc                                                <  2:3924766/140‑1 (MQ=255)
                                                                                            ttGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGGTCGTCCGTCTCGGCATCTGGTGTGTCAACTTTCTTAACAGGGATGTTAACCTGCTGGGTTTTTCCTCCCCCTAGTTGAAAAAGGgc                                                >  1:3916763/1‑140 (MQ=255)
                                                                                              gTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgctg                                              <  1:1719536/140‑1 (MQ=255)
                                                                                              gTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgctg                                              <  1:1770799/140‑1 (MQ=255)
                                                                                              gTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgctg                                              <  2:4719026/140‑1 (MQ=255)
                                                                                              gTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgctg                                              <  2:5012711/140‑1 (MQ=255)
                                                                                              gTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgctg                                              <  1:4071501/140‑1 (MQ=255)
                                                                                              gTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgctg                                              <  1:607539/140‑1 (MQ=255)
                                                                                               tCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGgctgc                                             <  1:666808/140‑1 (MQ=255)
                                                                                                  tCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTAc                                          <  2:4883727/140‑1 (MQ=255)
                                                                                                    tGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGGGCGTCCGGCTCGGCAGCTGGTGTGTCATAGTTATTAACAGGGATGATCACCGGCAGGGGTTTTGCTCCCCCGACTTTAAAAAGGGCGGCGACTg                                        >  1:2007393/1‑140 (MQ=255)
                                                                                                      tCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTGGGCTCTTCCGTCTCGGCATCTGGTGTGTCATCGTTATTAAAACGGATGATCACCAGCGGGGTTTTTGCTCCCCCCAGTTGGAAAAGGGCGGGTACTGTa                                      >  1:115263/1‑140 (MQ=255)
                                                                                                      tCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTGTTTCGGCTCTTACGTCTCGGCATCTGGTGTCTCATATTTGTTAACAGGGATGATGACCGGCTGGGTTTTTCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTa                                      >  2:802634/1‑140 (MQ=255)
                                                                                                             cTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCAt                               <  1:3173978/140‑1 (MQ=255)
                                                                                                               gATCACCCGCAGTTTCAGAAGAGTCGTTTTTAGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCg                             <  1:3691490/140‑1 (MQ=255)
                                                                                                                aTCACCCGCAGTTTCAGAAGAGTCGTGTTTGGGCTGTTGCGTCTGGGCATGAGGTGTCGGATCTTTCTTAAAAAGGATGATCACCAGCTGGGGTTTGCCTCCCCCGAGTTGAAAAAGGGCTGCTACGGTCTGTTCAACgg                            >  2:4589722/1‑139 (MQ=255)
                                                                                                                  cACCCGCAGTTTCAGAAGAGTCGTTTTTCGGGTCTTACGTGTCGGCATGTGGTGTGTCATCTTTATTAACAGGGATGTTCACCGGCTGGGTTTTGGCTCCCCCCACTTTTAAAAGGGCTGCTACTGTGTGTGCATCGTCt                          >  1:3866602/1‑140 (MQ=255)
                                                                                                                       gCAGTTTCAGAAGAGTCGTTTTTCGGCGCTTCCGTGTCGGCATCGGGTGTGTCATATTTGTTAAAAGGGATGATCACCGGCTGGGTTTTGCCTCCCCCGAGTTGTAAAAGGGCTGGTACTGTATGTTAAACGTCTTTTaa                     >  2:852901/1‑139 (MQ=255)
                                                                                                                          gTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATc                  <  1:735255/140‑1 (MQ=255)
                                                                                                                          gTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATc                  <  1:2607317/140‑1 (MQ=255)
                                                                                                                          gTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATc                  <  2:1387651/140‑1 (MQ=255)
                                                                                                                          gTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTCGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATc                  <  1:2753079/140‑1 (MQ=255)
                                                                                                                          gTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCCGCAGATGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATc                  <  2:2381756/140‑1 (MQ=255)
                                                                                                                              cAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCa              <  1:1229813/140‑1 (MQ=255)
                                                                                                                              cAGAAGAGTCGATTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTCACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGCGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCa              <  2:533005/140‑1 (MQ=255)
                                                                                                                              aagaagaTTGGTTTTTCGGCTCTTCAGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTATTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCa              <  1:2492732/139‑1 (MQ=255)
                                                                                                                                  agagTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTaa          <  1:1334002/140‑1 (MQ=255)
                                                                                                                                         tttttCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTAAACGTGGc   <  1:1598342/140‑1 (MQ=255)
                                                                                                                                         tttttCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTAAACGTGGc   <  2:1518825/140‑1 (MQ=255)
                                                                                                                                         tttttCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTAAACGTGGc   <  2:1590132/140‑1 (MQ=255)
                                                                                                                                          ttttCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTAAACGTGGCt  <  2:322279/140‑1 (MQ=255)
                                                                                                                                          ttttCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTAAACGTGGCt  <  2:3894658/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AATCATCACTCTTAGAAGAATCGCCACCCTTAGTGTCAGCTGATGCCTGTTGTTCTTCCTCTTTTTGCTCCTTGCTGACTGAAGGTGCAGATTTGTCATCTGTCCCTTCCTGATCACCCGCAGTTTCAGAAGAGTCGTTTTTCGGCTCTTCCGTCTCGGCATCTGGTGTCTCATCTTTCTTAACAGGGATGATCACCTGCTGGGTTTTGCCTCCCCCTAGTTGAAAAAGGGCTGCTACTGTATGTTCATCGTCTTTTACATCTGCAGTAAACGTGGCT  >  NZ_CP034943/3559207‑3559484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: