Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,562,015 A→C 12.5% intergenic (‑661/‑137) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,562,0150AC12.5% 561.9 / 7.7 261intergenic (‑661/‑137)EO946_RS18300/EO946_RS21105response regulator/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (125/82);  new base C (4/43);  total (129/132)
Fisher's exact test for biased strand distribution p-value = 2.25e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATC  >  NZ_CP034943/3561879‑3562151
                                                                                                                                         |                                                                                                                                        
aTCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                                                       <  1:932952/140‑1 (MQ=255)
aTCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                                                       <  2:1295199/140‑1 (MQ=255)
 tCAGCACGTCGTTTATTTACAGTGCAAATCGCATATATTTTAATGGCCAAGTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGt                                                                                                                                      <  2:1322838/140‑1 (MQ=255)
      aCGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACaa                                                                                                                                 <  1:1537595/140‑1 (MQ=255)
        gtcgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTGTATAGCGCAAATCAGACCATATACGAGCTTTTTTACGCTTAACAAGGGTGACAATg                                                                                                                               >  2:3793167/1‑140 (MQ=255)
         tcgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGa                                                                                                                              <  1:2946802/140‑1 (MQ=255)
         tcgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGa                                                                                                                              <  1:3573975/140‑1 (MQ=255)
          cgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGaa                                                                                                                             >  1:614288/1‑140 (MQ=255)
          cgtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGaa                                                                                                                             >  1:3710686/1‑140 (MQ=255)
           gtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAAc                                                                                                                            <  1:1240239/140‑1 (MQ=255)
             ttatttaCAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACtt                                                                                                                          >  2:1199539/1‑140 (MQ=255)
                    cAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAc                                                                                                                   <  1:2305636/140‑1 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  2:1918385/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  2:3219742/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:34744/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  2:2394192/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:3900441/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:3952681/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:5095272/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:4890189/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:516453/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:1176423/1‑140 (MQ=255)
                            aTCGCATCTATTTTAATGGCCAATTAATAGGACAACGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCa                                                                                                           <  2:3116752/140‑1 (MQ=255)
                                   cTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAAAATTTTCAAACttat                                                                                                    >  2:4221716/1‑138 (MQ=255)
                                           agtcacAATTAATAGCACAAACCGCACGCCCTAAAAATAAACGTTATTTTATACCCCAAACCCCCCCATATACGCGCTTTTTTACGCTGCACAAGGGTGACAATGAACTGGTGGTCAATTTTTCAAACTACGGAAAAACg                                                                                            <  1:1441853/135‑1 (MQ=255)
                                           aTGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACg                                                                                            >  1:4469909/1‑140 (MQ=255)
                                           aTGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACg                                                                                            >  1:1988575/1‑140 (MQ=255)
                                            tGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTTATTTTATACCGCAAAACACACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGc                                                                                           <  2:449470/140‑1 (MQ=255)
                                            tGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGc                                                                                           <  2:3631392/140‑1 (MQ=255)
                                                      aTAGGACAAAGCGGACGCCCTAAAAATAAACGTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACATTGACCTTGTGGTACAATTTACAAACTTCGGAAAAACGCCGGGGTgggg                                                                                 <  2:476959/140‑1 (MQ=255)
                                                      aTAGGACAAAGCGGACGCCATTCAAATAACCCTGATTTTATAGCGCAAAACAGACCATAGACGAGCTTTTTTCCGCTTCACAAGGGTGACACTGAACTTGTGGTACAATTTACAAACTTCGGAAAAACGCCGGGGTgggg                                                                                 <  2:4612702/140‑1 (MQ=255)
                                                        aGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGAAAAACAGACCATATACGAGCTATTTTACGGTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAAGTTCGTAAAAGCGCCCGGGTGGGGaa                                                                               >  2:4869942/1‑140 (MQ=255)
                                                             aaaGCGTAAGCCCTTCAAATAAACCTTTTTTTATAGCGAAAAACAGACCATTTCCGAGCTTTTTTACGCTTCACACGGGTGAAATTGAAGTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                          <  2:2876365/140‑1 (MQ=255)
                                                             aaaGCGCAAGCCCTTCAAATTAACCAGATTTTATAGCCCCAAACAGACCAGATACGAGCTTTTTTACGCTTCACACGGGTGACACTGAACTTGTGGTACACTGTTCAAACGTCGGACATACGCCGGGTTGTGGCATCGCa                                                                          >  2:3929634/1‑140 (MQ=255)
                                                             aaaGCGCAACCCATAAAAAAACACCTTATTTAATAGCCAAAAACACACCATATACGAGCTTTTTTACGCTTCAAACGGGTGACAATGAACTTGTGGTAAAATTTACAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCa                                                                          <  1:2826234/140‑1 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                        >  2:4804056/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                        >  2:375532/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                        >  1:4452740/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGATTTTTTACGCTTCACAAGGGTGACACTCAACTTGTGGTACAATTTTCAAAATTCGTAAACACGAGGGCGTGGGGAATCGCaaa                                                                        >  1:4559114/1‑140 (MQ=255)
                                                                 cGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGc                                                                      >  1:3541276/1‑140 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                   >  1:2671511/1‑140 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                   >  1:3028494/1‑140 (MQ=255)
                                                                         cTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACt                                                              >  1:1714297/1‑140 (MQ=255)
                                                                         cTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACt                                                              >  2:2041141/1‑140 (MQ=255)
                                                                         cTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACt                                                              >  1:3390320/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAAc                                                                                                                            <  1:1665206/76‑1 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                            >  1:4923058/1‑140 (MQ=255)
                                                                             aaaTAAACCTGATTTTATAGCGAAAAACACACCAAATCCGAGCTTTTTAACGCTTCACACGGGTGACAATGAACTTGTGTTAAAATTTCCAAAATTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTg                                                          <  1:1126334/140‑1 (MQ=255)
                                                                                aaaaCCTTATTTTATAGCGAAAAAACCCACATATCCGAGCTTTTTGACGCTACACACGGGTGACAATCAACTTGGGTTACAATTTTCAAAGTTGGCAAAAACGCCGGGGTGGGGAATCGCAACGCCGATTACTTTTGGaa                                                       <  1:2103831/139‑1 (MQ=255)
                                                                                  acccTTATTTTATAGCTCAAAACAGACAATATACGAGATTTTTTCCGGTTCCACAGGTTGAAACTCAAGTTGTGGTAAAATTTACAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3881638/138‑1 (MQ=255)
                                                                                  aaccataTTTTATAGCCCAAAACACACAATACCCGAGCTTTTTCACGATTCACCCGGGTTACAATGAAATTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:1046748/134‑1 (MQ=255)
                                                                                  aacattaTTTTATAGCGAAAACCAGACAATATACGAGCTTTTTTACGCTTCACAAGGGTTAAAATTAACTTGTGGTACAATTTACAAACTACGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3771677/134‑1 (MQ=255)
                                                                                  aacattaTTTTATAGCGAAAAACACACAATATACGAGCTTTTTTACGCTTCACACGGGTGAAAATGAACTTGTGGTACAATTTTAAAAATTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:1304430/134‑1 (MQ=255)
                                                                                  aaCCTTATTTTATCGCCCAAAACCCACCATATACCAGCTTTTTTACGCTTCACACGGGTTACAATGAAGTTGTGTTACAATTTTCAAACTTCGGAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:749969/140‑1 (MQ=255)
                                                                                  aaCCTTATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGAAAATCAACTTGTGGTACAATTTTCAAACGTCGCAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:4804056/140‑1 (MQ=255)
                                                                                  aaCCTTATTTTATAGCCAAAAACACACAATATACGAGCTTTTTTCCGATTCACACGGGTGAAAATGAAGTTGTGGTACAATTTTCAAACTGCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:1025655/140‑1 (MQ=255)
                                                                                  aaCCTTATTTTATACCGAAAACCACCCAATATACGAGTTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:4180742/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGCCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATTAACTTGTGGTACAATTTTAAAAATTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  2:926981/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGAAAATGAACTTGGGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:1298948/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACACACAATATACGAGATTTTTTACGCTTCACAAGGGTGACAATCAACTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  2:2206796/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCCCAAAACCCACCATATACCAGCTTTTGTACGCTTCACACGGGTGACAATGAACGTGTGGTACAATTTTCAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:917112/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCCAAAACAACCCATTAAACTAGATTTTTGCCGCTTCAAACGGGTGAAATTTACCTTGTGGTAAAATTTTCAAACTGGGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:1771606/140‑1 (MQ=255)
                                                                                  aaCCGGATTTTATAGCCCAAACCCGCCCATATACGAGGTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGTTACAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAACCCGATTACTTTTGGAACg                                                     <  1:4000524/140‑1 (MQ=255)
                                                                                   aCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                                                    >  2:1240923/1‑140 (MQ=255)
                                                                                    ccTGTTTTTATCGCCCAAAACCCACCATACACGAGCTTTTTTACGCTACACACGGGTGAAAATGAACTTGTGGAACAATTTCCAAACGTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                   <  1:2568719/140‑1 (MQ=255)
                                                                                      tGATTTTATAGCGAAAAACAGACCATCTACGAGTTTTTTTACGATTAACCTGGGTGACATTTAACTTGTGTTAAAATTTTAAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                 <  1:5211396/140‑1 (MQ=255)
                                                                                        aTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                               >  1:4678991/1‑140 (MQ=255)
                                                                                          tttATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                             <  2:2596227/140‑1 (MQ=255)
                                                                                          tttATAGCGCAAAACACACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                             <  2:6672/140‑1 (MQ=255)
                                                                                          tttATAGCGAAAAACCGACCATATACGATCTTTTTTACGCTTAACAAGGGTGACAATGACCTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATaa                                             <  2:3328463/140‑1 (MQ=255)
                                                                                                  gcAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATAtttc                                     >  2:3822497/1‑138 (MQ=255)
                                                                                                    aaaaCAGACCATATACGAGATTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCga                                   <  2:5085840/140‑1 (MQ=255)
                                                                                                      aaCAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCCGGATAACATATTGCgaga                                 >  2:1745946/1‑140 (MQ=255)
                                                                                                         aGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGaa                              <  2:2338097/140‑1 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAATACGCGGGGGGGGGGAATCGCAAAGCCGATTACTTGTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  1:3190852/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  2:1681231/1‑140 (MQ=255)
                                                                                                                tataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                   <  2:2685490/113‑1 (MQ=255)
                                                                                                                 ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:2732231/1‑140 (MQ=255)
                                                                                                                 ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:1865918/1‑140 (MQ=255)
                                                                                                                   tcGAGCTTTTTTACGCTTCACTAGGGTGACAATGAATTTGTTTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGTGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTc                     <  2:1089448/139‑1 (MQ=255)
                                                                                                                   aCGAGCTTTTTTACGCTTCACAAGGGGGACAATGAACTTGTGGTACAATTTTCAAACTGCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTc                     <  2:1861663/140‑1 (MQ=255)
                                                                                                                      aGCTTTTTTACGCTTCACAAGGGTGACAATGAAATTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCTAGAGAACAAAATTTCCtt                  >  2:2825878/1‑140 (MQ=255)
                                                                                                                       gCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCttt                 >  2:3891947/1‑140 (MQ=255)
                                                                                                                        cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTc                >  1:504059/1‑140 (MQ=255)
                                                                                                                        cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTc                >  2:3236638/1‑140 (MQ=255)
                                                                                                                          tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt              >  1:3629602/1‑140 (MQ=255)
                                                                                                                           ttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTa             >  1:4144413/1‑140 (MQ=255)
                                                                                                                           ttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTa             >  2:1495167/1‑140 (MQ=255)
                                                                                                                            tttACGCTTCACACGGGTTACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa            <  2:4326545/140‑1 (MQ=255)
                                                                                                                            tttACGCTTCACAAGGGTGACAATTAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa            <  2:1617251/140‑1 (MQ=255)
                                                                                                                                     cacaAGGGTGACAATGAACTTTTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   <  2:4004784/140‑1 (MQ=255)
                                                                                                                                     cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTgggg                                                                                 <  1:2425744/62‑1 (MQ=255)
                                                                                                                                     cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTgggg                                                                                 >  2:2425744/1‑62 (MQ=255)
                                                                                                                                     cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   >  1:2134263/1‑140 (MQ=255)
                                                                                                                                     cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   <  2:3490858/140‑1 (MQ=255)
                                                                                                                                     cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   >  1:4337731/1‑140 (MQ=255)
                                                                                                                                      acacGGGTGACAATGAACTTGTGGTACAATTTACAAACTTCGGAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATc  <  1:1918385/140‑1 (MQ=255)
                                                                                                                                      acaAGGGTGACAATGACGTTGTGGTACACTTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATc  <  2:4124052/140‑1 (MQ=255)
                                                                                                                                      acaAGGGTGAAAATGAACTTGTGGTACAATTTACAAACTTGGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATc  <  1:2233593/140‑1 (MQ=255)
                                                                                                                                      acaAGGGTGAAAATGAAATTGTGTTACAATTTACAAACTTCGCAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATc  <  1:2041141/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATC  >  NZ_CP034943/3561879‑3562151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: