Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,584,316 G→C 7.9% E333Q (GAA→CAA)  EO946_RS18420 → N‑acetylneuraminate synthase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,584,3160GC 7.9% 999.8 / 11.2 433E333Q (GAA→CAA) EO946_RS18420N‑acetylneuraminate synthase family protein
Reads supporting (aligned to +/- strand):  ref base G (182/182);  new base C (2/39);  total (185/248)
Fisher's exact test for biased strand distribution p-value = 2.57e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

ATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGGTG  >  NZ_CP034943/3584184‑3584454
                                                                                                                                    |                                                                                                                                          
aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                                                     <  1:785080/140‑1 (MQ=255)
aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                                                     <  1:5236179/140‑1 (MQ=255)
      acacTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCt                                                                                                                               <  2:3652500/140‑1 (MQ=255)
          tGAATAAAGCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAACAAGTCTCAGa                                                                                                                           >  1:267948/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  2:833863/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  2:2225061/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  1:1221004/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  1:1360938/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  2:2073923/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         <  1:5013257/140‑1 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  1:4287223/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  1:4176179/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  1:1845891/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  1:1874245/1‑140 (MQ=255)
            aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  1:3993376/1‑140 (MQ=255)
             aTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCATCGAAATAAAAAGTCTAAGAAtt                                                                                                                        >  2:905400/1‑140 (MQ=255)
             aTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAAtt                                                                                                                        >  2:606499/1‑140 (MQ=255)
             aTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTGAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAtt                                                                                                                        >  2:4874163/1‑140 (MQ=255)
                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                                    <  2:4678491/140‑1 (MQ=255)
                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                                    <  1:2073923/140‑1 (MQ=255)
                 aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                                    <  1:3481202/140‑1 (MQ=255)
                    aaGCTCCAAAACATGCTGAAATGAGAATGCATTAATGCATTTACAAGCCGAGGACGGATTTCCTAAAGTAGCAACTTCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                                                 <  2:3377202/140‑1 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:1611564/1‑140 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               <  2:1492511/140‑1 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               <  2:2388653/140‑1 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:4055149/1‑140 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  2:2574001/1‑140 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               <  2:3510640/140‑1 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:2109496/1‑140 (MQ=255)
                      gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATGTCCTCAAGTAGCAACTGCTTTTTTATCGTTCCACGAGCCCATTACACCCAACGAAATAAAAGGTCTACGACTTGGGAGTGcc                                                                                                               >  1:3192930/1‑139 (MQ=255)
                       cTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAACTCTAAGAATTGCGATTGCAg                                                                                                              <  1:2939180/140‑1 (MQ=255)
                        tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                                             >  2:4547860/1‑140 (MQ=255)
                        tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                                             >  2:5031861/1‑140 (MQ=255)
                              aGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCAGTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATg                                                                                                       <  2:2181778/140‑1 (MQ=255)
                                aTGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTa                                                                                                     >  2:4074137/1‑140 (MQ=255)
                                  gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                                                   >  2:4603398/1‑140 (MQ=255)
                                       aaTGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTaaa                                                                                              >  1:2482181/1‑140 (MQ=255)
                                                 cATTAATGCCTTTACAAGCCGAGGACGCATTTCCTAAAGTAGCAACTGCTGATTTATCGTAAAACCAGCCCATTACCCCCAACCAAATAAAAAGTCTAATAATGGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGt                                                                                    <  1:1505386/140‑1 (MQ=255)
                                                      aTGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACACGTCTGcc                                                                               >  2:3245199/1‑140 (MQ=255)
                                                       tGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCg                                                                              >  1:1452701/1‑140 (MQ=255)
                                                         ccTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                                                 >  2:560572/1‑103 (MQ=255)
                                                           tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaa                                                                          >  2:2499583/1‑140 (MQ=255)
                                                            ttACAAGCCGAGGACGCATTTCCTCAAGTATCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAACTAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTCCCGaaaaa                                                                         >  1:5187894/1‑140 (MQ=255)
                                                            ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                                                         >  2:3731280/1‑140 (MQ=255)
                                                                  gCCGCGGACGCATTCCCTCAAGTAGCAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTACGATTTGCGTTTGCTGTGATTTGTCGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgct                                                                   <  1:703492/140‑1 (MQ=255)
                                                                  gCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCgct                                                                   <  1:4513506/140‑1 (MQ=255)
                                                                  cccGAGGACGCATTTCCTCAAGTAGCAACGGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAACGTATAAGAAGGGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgct                                                                   <  1:1142254/139‑1 (MQ=255)
                                                                   ccGAGTACGCATTTCCTAAAGTAGCAACTGCTGTTTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctg                                                                  <  2:1331130/140‑1 (MQ=255)
                                                                    cgcggACGCATTTCCTCAAGTAGCAACTGATGATTTATCTTTAAACCAGCCCATTACACCCACCGAAATAAAAAGTCTAAGAATTGCTATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                                                 <  1:1003462/137‑1 (MQ=255)
                                                                      cggACGCATTTCCTCAAGTATCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                                               <  2:3969409/139‑1 (MQ=255)
                                                                      cggACGCATTTCCACAAGTAGCAACTGCTGATTTATCGTAAAACCAGCCCATTACACCCAACGAAATACACAGTGTAAGAATTGCGATTGCAGTGATATGTAGGCTAACATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                                               <  2:3847753/139‑1 (MQ=255)
                                                                       ggACGCATTTCCTCAAGTAGCAACTGCTTTTTTATCGTTAAACCAGCCAATTACACCCAACCAAATAAAAAGTCTAATAATTGCGATTGCAGTGATATTTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                                              <  1:1730003/140‑1 (MQ=255)
                                                                       ggACGCATTTCCTCAAGTAGCAACTGCTTATTTATCTTTAAACCCGCCAATTACACCCAACTAAATAAAAATTATAATAATTGCTATTGCTGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                                              <  1:1158428/140‑1 (MQ=255)
                                                                       ggACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                                              <  2:3162446/140‑1 (MQ=255)
                                                                       ggACGCATTTCCTAAAGTAGCAACTGCTGATTTATCGTAAAACCCCCCCATTACCCCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCCGTCATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                                              <  1:3600908/140‑1 (MQ=255)
                                                                            cttttCCACACGTACCAACTGCTGATTTATCTTTAAACCAGCCCATTCCACCCAACTAAATAAAACGTATAAGAATTGCGTTTGCAGTTATATGTCGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                                                         <  2:1064279/138‑1 (MQ=255)
                                                                            aaTTTCCTCAAGTAGCAACTGCTTATTTATCGTTAAACCCGCCCATTCCACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                                                         <  2:2306392/139‑1 (MQ=255)
                                                                             aTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAGAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAACGCGCTGCTCCCTCttt                                                        >  1:2720649/1‑140 (MQ=255)
                                                                              tttCCTCAAGTAGCAACTGCTTATGTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  2:1220333/140‑1 (MQ=255)
                                                                              tttCCTCAAGTAGCAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATTGCTATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  1:3572753/140‑1 (MQ=255)
                                                                              tttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTCCACCCAACCAAATAAAAAGACTAAGAATTGCGATTGCAGTGATTTGTCGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  1:3688838/140‑1 (MQ=255)
                                                                              tttCCTAAAGTAGAAACTGCTTATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  1:2402952/140‑1 (MQ=255)
                                                                              tttCCTAAAGTAGAAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAACACTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCACCCTATTTa                                                       <  2:195627/140‑1 (MQ=255)
                                                                              tttCCACAATTAGCACCTGCTTATTTATCTTTAAACCAGCCCATTACCCCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  1:968271/140‑1 (MQ=255)
                                                                                  cTCAAGTAGCAACTGCTTATTTATCGTTAAACCCGCCCATTACACCCAACGAAAAAAAAAGTATAAGAATTGCTATTGCAGTGATATGTCGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                                                   <  1:493087/140‑1 (MQ=255)
                                                                                  cTCAAGTAGAAACTGCTTATTTATCTTTAACCCACCCCTTTACACCCAACCAAATAAAAATTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                                                   <  1:2436668/140‑1 (MQ=255)
                                                                                    aaaGTAGAAACTGCTTATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCTGTGTTATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtat                                                 <  2:4335364/139‑1 (MQ=255)
                                                                                     aaGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACTGtata                                                >  2:2687041/1‑140 (MQ=255)
                                                                                     aaGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTACCTATTTACGGtata                                                >  2:4127599/1‑140 (MQ=255)
                                                                                         aGCAACTGCTGATTTATCGATAAACCAGCCAATACCCCCCACCGAACAAAAACGTCTACGAATTGCGATTGCAGTTATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt                                            <  1:1527124/140‑1 (MQ=255)
                                                                                           cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTATGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTCTTTACGGTATACCAACt                                          >  2:5154694/1‑140 (MQ=255)
                                                                                           cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAAGGTATACCATCt                                          >  2:3009137/1‑140 (MQ=255)
                                                                                           cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTAGTCTGCCGAAAAAAGCGCTGCTCACTATTTAAGGTATACAATCt                                          >  2:2869947/1‑140 (MQ=255)
                                                                                                       tATCGTTAAACCCCCCCATTACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGt                              <  1:2289629/140‑1 (MQ=255)
                                                                                                         tCGATAAACCAGCCCATAACACACAACGAAATAACAAGACTAAGAATTGCGAGTGCAGTCATAGGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGttt                            <  1:4336719/140‑1 (MQ=255)
                                                                                                          cGTTAAACCAGCCCATTACACCCAACGAACTAAAAACTCTAAGAATTCCGATTGCAGTCATATGAAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTa                           <  1:4706579/140‑1 (MQ=255)
                                                                                                             tAAACCCCCCCATTACCCCAACCCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATc                        <  1:4805759/140‑1 (MQ=255)
                                                                                                             tAAACCAGCCCATTACACCCAACGAAATAAACAGTCTAAGATTTCCGATTGAAGTGATTTGTTGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATc                        <  2:3283099/140‑1 (MQ=255)
                                                                                                              aaaCCAGCCCATTACACCCAACGAAATAAAAAGTCTACGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCa                       >  1:3908145/1‑140 (MQ=255)
                                                                                                              aaaCCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTCGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTCTTTAATCa                       >  1:3090110/1‑140 (MQ=255)
                                                                                                              aaaCCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCa                       >  1:3865553/1‑140 (MQ=255)
                                                                                                               aaCCAGCCCATTACACCAAACCAAATAAAAAGTCTAAGAATTCCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCaa                      <  1:1702655/140‑1 (MQ=255)
                                                                                                                    gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCTATTTCAGTGATATGTATGCTAAAATCGACTCGTCTGCCGAAAAAATCGCTGCTCACTATTTACGGTATACCATCTGTTTAACGCTGTTTAATAAATTGcc                 >  2:401526/1‑140 (MQ=255)
                                                                                                                    gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAATCGCTGCTCCCTATTTACGGTATAACATCTGTTGAACGCTGTTTAATCAATTGca                 >  2:4409550/1‑139 (MQ=255)
                                                                                                                    gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGcc                 >  2:1552525/1‑140 (MQ=255)
                                                                                                                    gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGcc                 >  2:2330577/1‑140 (MQ=255)
                                                                                                                    gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACCCTGTTTAATCAATTGcc                 >  2:4950013/1‑140 (MQ=255)
                                                                                                                        aTTACCCCCCCCGAAAGAAAAAGTCTAACAATGGCGATTGCAGTCTTAGGTAGGCAAACAGCCACTCCTCGGCCGAAAAAAGCGCTGCTCCCTATTGACGGTATACCAGCTGTTGAACGCTGTTTAATCAATTGCCGGGc             <  2:2839137/140‑1 (MQ=255)
                                                                                                                        aTTACACCCCACCAAATAAAAAGTCTAAGACTTGCGATTGCAGTGATATTTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGc             <  1:3255403/140‑1 (MQ=255)
                                                                                                                        aTTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGc             <  2:1137386/140‑1 (MQ=255)
                                                                                                                        aTTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGc             <  2:186587/140‑1 (MQ=255)
                                                                                                                        aTTAAACCCAACCAAATAAAAAGTCTAATAATTGCGATTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGc             <  1:2256010/140‑1 (MQ=255)
                                                                                                                          tACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                                          >  1:2503869/1‑93 (MQ=255)
                                                                                                                                cAACCAAATAAAAAGTCTAAGACTTGCGATTGCAGTTATATTTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTg     <  1:4876576/140‑1 (MQ=255)
                                                                                                                                 aaCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTgg    >  2:1771574/1‑140 (MQ=255)
                                                                                                                                 aaCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTgg    >  1:3762956/1‑140 (MQ=255)
                                                                                                                                 aaCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTgg    >  1:4090101/1‑140 (MQ=255)
                                                                                                                                  aCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCTACTCGTCTGCCTAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCATGGCTTTCCCTGgt   >  1:1082865/1‑140 (MQ=255)
                                                                                                                                  aCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGgt   <  1:4648885/140‑1 (MQ=255)
                                                                                                                                  aCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGgt   >  1:254570/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                          
ATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGGTG  >  NZ_CP034943/3584184‑3584454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: