BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,962	C→A	5.5%	D36Y (GAT→TAT)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,962	0	C	A	5.5%	654.5 / 5.1	245	D36Y (GAT→TAT)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base C (125/98); new base A (17/5); total (142/103)
Fisher's exact test for biased strand distribution p-value = 6.98e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTAT  >  NZ_CP034943/3746823‑3747100

tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                                                                                            <  1:2751534/140‑1 (MQ=255)

tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                                                                                            <  2:3600645/140‑1 (MQ=255)

    cTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAACATCAGCATATT‑TAACCCGTTTAAGCGCGCACGGAAACACAAGCCCGCCCGTAAAAACTGtt                                                                                                                                        >  1:3897035/1‑139 (MQ=255)

           gCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTtctc                                                                                                                                 <  2:1535408/140‑1 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCAAATG‑TACCCATTGTCAGCGCGCACGGAAACACATTCCCGCCGGTATAATATGTATGTTATcc                                                                                                                                >  2:2601438/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTAGGCAAAAGCAGCATAAG‑TACCCCAGGTCAGCGCGCACCGAAACACAAGCCCGCCGTTATAAACTGTCTGTTCTcc                                                                                                                                >  2:3066894/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAAACCACCATAAT‑TAACCAATTTCAGCGCGCACCGCAAAACAAACCCCCCCCTATAAAAAGTATGTtctgc                                                                                                                                >  1:1470772/1‑138 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAAAACCACCAAAAT‑TAACCAATTTAAGCGCGCACCGAAACACAATCCCCCCCTTATAAAATTTATGTtatct                                                                                                                                >  2:4518405/1‑139 (MQ=255)

               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                                                             <  2:42625/140‑1 (MQ=255)

                 cTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCCAATG‑TTACCAATGTCAGCGCGCACGGAAACACAAGCCCCCCGGTATAATCTGTATGTTATCCAATCa                                                                                                                           >  2:657109/1‑140 (MQ=255)

                   tGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGa                                                                                                                         <  1:657109/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACCTCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                                                                                      <  2:4346061/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TGCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                                                                                      <  1:2142146/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                                                                                      <  2:2861397/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATATGTATGTTCTCCAATCAGATTc                                                                                                                      <  1:3852363/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCGTTATTCAACAACCGCCTATGTTACCCATT‑TCAGCGCCCACCGAAACACATGCCCCCCGGGATAATCTGTATGTTCTCCAATCAGATTc                                                                                                                      >  1:3100106/1‑140 (MQ=255)

                       cccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCa                                                                                                                     <  1:4259186/140‑1 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAAAGCAGCATATG‑TACCCATTGTCAGCGCCCACGGAAACACATGCCCGCCGTTATAAACAGTATGTACTCCAAACACATACActct                                                                                                                 >  2:2852029/1‑140 (MQ=255)

                                tttAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCGTTGTTCAACAGCAGCATATT‑TACCCCATTTCAGCCCGCACGGGAAAACAAGCCCGCCGGTATAATATTGTTGTTGTGCGATCAGAATCAAACTTGCAg                                                                                                            >  1:3364491/1‑140 (MQ=255)

                                  tAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTGCAAAAGCCGCATAAT‑TAACCCGTTTCAGCGCGCACGGGAACACAAGGCCGCCGTTAAAATATTTATTTTGTCCAAGCAGATTAAAAATTCCAGGt                                                                                                          >  1:1126249/1‑140 (MQ=255)

                                         gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGGAACAGCAGCATATG‑TACCCCGTGTCAGCGCGCACGGAAACACAAGCCCGCCCTTATAATCTGTATGTTCTCCAAACAGATTCACACTTCCAGGTGCGaaaa                                                                                                   >  2:4294743/1‑140 (MQ=255)

                                         gTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTTCAACAGCAGCATATG‑TACCCATTTTAAGCCCGCACCGACACACAAGCCCGCCCTTATAAACTGTATGTTCTCCAAACAGAATAACTCTTCCAGGGGctgtct                                                                                                   >  2:3440541/1‑135 (MQ=255)

                                          tCTGAATACATCCGGACAACCCCGGCGTTTGTTTATGAAACAGCAACAAAAT‑TTCCCCGTTTCCGCGCCCACGGAAACACCTGCCCGCCGGTATAATAGGGAAGTTCTGCCATCACTTTCAGGCTTCCAGGCGCGaaaaa                                                                                                  >  1:1076499/1‑140 (MQ=255)

                                                    tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc                                                                                        <  2:5170673/140‑1 (MQ=255)

                                                        gACAACCCCGTCGTTTCTTTCTGCAACAGCAGCCTATG‑TAACCATTGTGAGCGCGCACCGAAAAACCTGCCCGCCGTTATAATCTGTATGTTATCCGAACAGTTTGAAGCTTGCAGGTGCGAAAAAAAACCGTAACACct                                                                                    >  2:3506737/1‑140 (MQ=255)

                                                             ccccGTCGTTTGTTTCTGCAACAGCACCATATG‑TAACCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTGATAAAAGGTATGTTCTCCAATCAGATTAACTCTTACACGTTCCAAAAAAAACCGGAACCCCGCtttt                                                                               >  2:3287836/1‑140 (MQ=255)

                                                             ccccGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACCGAAAAAAAAGCCCCCCGGTAAAAAATGTATTTTCTCCAAGCCGATTCAATCTTACAGGTGCGAAAAAAAAACGGAACCCCGCtttt                                                                               >  2:4604520/1‑140 (MQ=255)

                                                             ccccGTCGTTTCTTTCTGCAACAGCAGCAAATG‑TACCCCATTTCAGCGCGCACGGAAACACATGCCCCCCGTTATAATCAGTATGTTCTCCAAACAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCtttt                                                                               >  2:2432593/1‑140 (MQ=255)

                                                                  tcgtTTCTTTCTGCAACAGCAGAATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCTTTATAATATGTATGTTCTCCAAACAGATTCACTCTTCCAGGTGCTAAAAAAAACCGTAACCCCTCTTTTTCTcc                                                                          <  2:3779338/140‑1 (MQ=255)

                                                                         tttctGCAACAGCAGCAAATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAAACGTAACCCCTCTTTTTATACGAATaaa                                                                   >  2:2468411/1‑138 (MQ=255)

                                                                           tctGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACt                                                                 <  1:1151606/140‑1 (MQ=255)

                                                                           tctGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTATTTTTCTCCCAATACACt                                                                 >  2:4417303/1‑140 (MQ=255)

                                                                                aaCAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCaa                                                            <  2:2544308/140‑1 (MQ=255)

                                                                                   agcagcATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                         <  2:849641/140‑1 (MQ=255)

                                                                                    gcagcaTATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAatat                                                        <  2:6097/140‑1 (MQ=255)

                                                                                    gcagcaTATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:1291502/140‑1 (MQ=255)

                                                                                     cagcaTATG‑TACCCATTGTCAGCGCGCACGGATACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:1825171/140‑1 (MQ=255)

                                                                                     cagcaTATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCGCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:3643105/140‑1 (MQ=255)

                                                                                     cagcaTATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  2:3023490/140‑1 (MQ=255)

                                                                                     cagcaTATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:1217541/140‑1 (MQ=255)

                                                                                     cagcaTATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:1995165/140‑1 (MQ=255)

                                                                                     cagcaTATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  2:3678984/140‑1 (MQ=255)

                                                                                     cagcaTATG‑GACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  2:1918613/140‑1 (MQ=255)

                                                                                          tatG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTCACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCtt                                                  >  1:1625480/1‑140 (MQ=255)

                                                                                             g‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                               <  1:4586461/140‑1 (MQ=255)

                                                                                             g‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                               >  2:1830593/1‑140 (MQ=255)

                                                                                                ccccATTGTCAGCGCGCACGGACACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAAAACACTTGCAACTATCCCCTTTAGct                                             >  2:4089652/2‑140 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCTGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             <  2:5031191/140‑1 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             >  2:4028590/1‑140 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             >  2:4491729/1‑140 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             >  2:1199868/1‑140 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             >  2:176704/1‑140 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATATGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             <  2:3996321/140‑1 (MQ=255)

                                                                                                 cccATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCACATATCCCCTTTAGctc                                            >  2:1369848/1‑140 (MQ=255)

                                                                                                 cccATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGctc                                            >  1:3411616/1‑140 (MQ=255)

                                                                                                 cccATTGTCAGCCCGCACGGAAACACATGCCCGCCGTAATAAACTGTATGTTCTCCAATCAGAGTCACACTTCCAGGTGCGAAAAAAAACCGAAACCCCTCAATTACTCCCAATACACTTGCAAATATCCCCTTAACctc                                            >  2:1241639/1‑140 (MQ=255)

                                                                                                   cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  1:3033912/1‑140 (MQ=255)

                                                                                                   cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  1:3242988/1‑140 (MQ=255)

                                                                                                   cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          <  2:1076499/140‑1 (MQ=255)

                                                                                                   cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  1:554016/1‑140 (MQ=255)

                                                                                                   cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGAGTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAATTATACGCTTTAGCTCtt                                          >  1:604365/1‑140 (MQ=255)

                                                                                                    aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCACAGATCCCCTTTAGCTCTTc                                         >  1:3494187/1‑140 (MQ=255)

                                                                                                    aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTc                                         >  1:5102409/1‑140 (MQ=255)

                                                                                                    aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAACCGTAACCCCTCTGTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                        >  1:4858851/1‑140 (MQ=255)

                                                                                                     ttGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                        >  1:2860540/1‑140 (MQ=255)

                                                                                                     ttGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                        <  2:2085407/140‑1 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGACTACACGTGCAAATATCCCCTTTAGCTCTTccc                                       >  2:3314334/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAatat                                                        <  2:222600/123‑1 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  2:1950688/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  2:2251292/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  1:4060291/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  1:4616773/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  1:4346653/1‑140 (MQ=255)

                                                                                                       gTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCg                                      <  2:3113047/140‑1 (MQ=255)

                                                                                                       gTCAGCGCCCACGGAAACACATGACCGCCGTTATACTCTCAATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCg                                      <  2:4119147/140‑1 (MQ=255)

                                                                                                          aGCGCGCACGGAAACACATGCACGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACAGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCGCCTTTAGCTCTTCCCGCAt                                   >  2:1424425/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATTCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  1:2294539/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTACCCCCTCTTTTTGTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  1:4813666/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  1:1017741/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  2:799093/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  1:257130/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  1:2632679/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  1:4132446/1‑140 (MQ=255)

                                                                                                                cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                             >  1:1439693/1‑140 (MQ=255)

                                                                                                                 aCGTAAACACATGCCCGCCGTTATAATCTGTATGTTCGCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAAt                            <  2:3168654/140‑1 (MQ=255)

                                                                                                                         acaTGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTc                    >  2:1797255/1‑140 (MQ=255)

                                                                                                                           aTGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCAt                  >  2:702442/1‑140 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAATAACCGTAACCCCTCTTTTTCTCCGAATCCACTTGCAAATCTCCCCGTTAGCTCTTCCCGCATGTGCAATCACCGGTCAtgg                >  2:4964974/1‑138 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAATCCGTAACCCCTCTTTTTCTCCGACTACACTTGCAAATATCGCCGTTAGCTCTTCCCGCCTGTGCAATCACCTTTCaggg                >  1:3979133/1‑137 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                     >  2:3529853/1‑87 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                     <  1:3529853/87‑1 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTg                >  1:443861/1‑140 (MQ=255)

                                                                                                                               ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGtt              >  1:925742/1‑140 (MQ=255)

                                                                                                                               ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGtt              >  2:2797399/1‑140 (MQ=255)

                                                                                                                               ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTACAGGTGCGAAAAAAAACCCAAAAACCACTTTTTCTCCGAATACACTTGCAAATAACCCCTTTAACTCTTCCAGCATGTTCACACACCATTCAtattt              >  1:4978457/1‑136 (MQ=255)

                                                                                                                                  ccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGtttta           >  2:1617302/1‑140 (MQ=255)

                                                                                                                                       ataATCTTTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttattttt      <  1:2056253/140‑1 (MQ=255)

                                                                                                                                           tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat  <  1:4028285/140‑1 (MQ=255)

                                                                                                                                           tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat  <  1:3622371/140‑1 (MQ=255)

                                                                                                                                           tCTGTATGTTCTACAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat  <  2:3900124/140‑1 (MQ=255)

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATG‑TACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTAT  >  NZ_CP034943/3746823‑3747100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`