Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 135,117 G→T 6.5% intergenic (+164/‑74) EO946_RS00715 → / → rpsJ alpha/beta hydrolase/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943135,1170GT 6.5% 1083.8 / 8.1 451intergenic (+164/‑74)EO946_RS00715/rpsJalpha/beta hydrolase/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base G (206/196);  new base T (47/2);  total (253/198)
Fisher's exact test for biased strand distribution p-value = 7.90e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 457 total aligned reads displayed.

CACCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCA  >  NZ_CP034943/134978‑135256
                                                                                                                                            |                                                                                                                                           
 acCTGTATAATAAAAAAAGTGTGCAAACCATGAATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            <  2:565795/140‑1 (MQ=255)
      tataATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTgg                                                                                                                                       >  1:2466300/1‑140 (MQ=255)
      tataATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATgg                                                                                                                                       >  1:3928699/1‑140 (MQ=255)
          ataaAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCaca                                                                                                                                   >  2:4546165/1‑138 (MQ=255)
           taaAAAAAGTGTGCAAAAAATGCATAATTTAAATAAGTCTTGCAACATAAGTATATTTTCTGTATAATGTTGTATGTTGTTCTTTGACTGCGATGAAGTGAGAGGTTGCTGAAACAAACGGCAGCTTTTACATGGCAAAg                                                                                                                                  >  2:1037210/1‑140 (MQ=255)
             aaaaaaGTGTGCAAACCATGCATAGTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGgt                                                                                                                                >  2:3955416/1‑140 (MQ=255)
                aaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGtt                                                                                                                             >  2:745596/1‑140 (MQ=255)
                      tgCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTgg                                                                                                                                            >  1:3128140/1‑118 (MQ=255)
                      tgCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            <  2:3128140/119‑1 (MQ=255)
                      tgCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGtt                                                                                                                       >  2:3646112/1‑140 (MQ=255)
                          aaacATGCATATTTTAAATAAGTCTTGCAAAATACGTATATTTTCTGTAGAATGTTGTATGTTGTTCTTTGACTGCGATGAAGTGAGATGTTGCTGACACACCCGGCCGCGTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  2:4326912/137‑1 (MQ=255)
                          aaCCATTCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTTTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  2:5166803/140‑1 (MQ=255)
                          aaCCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  1:3812842/140‑1 (MQ=255)
                               tGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACg                                                                                                              <  1:4410050/140‑1 (MQ=255)
                                  atatTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGgag                                                                                                           <  2:3984232/140‑1 (MQ=255)
                                    atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:59069/140‑1 (MQ=255)
                                    atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  2:3209007/140‑1 (MQ=255)
                                    atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:1234240/140‑1 (MQ=255)
                                    atTTTAAATAAGTCGTGCAACATACGTCTATTTTCTGTATAAGGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  2:3442014/140‑1 (MQ=255)
                                    agtttAAATAAGTCTTGCAACATACGTCTATTTTCGGTATAATGGTGTATGATGGTCTTTGACTGCGATGAAGTGAGAGGGTGCTGACACACCCGGCCGCATTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:466885/138‑1 (MQ=255)
                                     ttttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGTTTTTCAGGTTTTTGTCAAGGTGaaa                                                                                                        >  1:5089547/1‑139 (MQ=255)
                                      tttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACt                                                                                                       >  2:121529/1‑140 (MQ=255)
                                       ttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATTAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCTTGTCAAAGGTGTTCAGGGTTTTATCACGGAGAACTg                                                                                                      >  2:1885941/1‑140 (MQ=255)
                                       ttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTGAGGTTTTTGTCAAGGGGAAcag                                                                                                      >  1:1556548/1‑138 (MQ=255)
                                         aaaTAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCAAGGTGTTCAGGTTTTTATCACGGAGAACTGTc                                                                                                    >  1:4055936/1‑140 (MQ=255)
                                                  ttGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCCTTGCAAAGGGTTTGAGGTTTTTATCACGGAGAAATGTCTAACTTTAt                                                                                           >  1:1768917/1‑140 (MQ=255)
                                                    gCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCCAAGGTGTTCAGGTTTTTATAACGGAGAACTGTCTAACTTGATGt                                                                                         >  1:3189220/1‑140 (MQ=255)
                                                    gCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCACAGGTGTTCAGGTTTTTGTCACGGAGACATGTGTAACTtgttgt                                                                                         >  1:2986834/1‑140 (MQ=255)
                                                    gCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGt                                                                                         <  1:3841864/140‑1 (MQ=255)
                                                          tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCACGGTGTTGAGGTTTTTCTGACGGAGAACTGTCTAACTTGATGTAGGCGa                                                                                   >  1:493920/1‑140 (MQ=255)
                                                          tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTGTGACGGAGAACTGTGTAAGTTGATGTTTGCGa                                                                                   >  1:2190975/1‑140 (MQ=255)
                                                               cTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAagg                                                                              >  2:151363/1‑140 (MQ=255)
                                                                  ttttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCAAAGGTGTTCAGGTTTTTGTCACGGAGTACTGTCTAACTTGATTTATGCGAAAaggagg                                                                           >  1:5186953/1‑140 (MQ=255)
                                                                  ttttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTGACGTTTTTATAAACGAGAACAGTCTAAAATTATGTAGTCGAAAaggagg                                                                           >  1:2804799/1‑140 (MQ=255)
                                                                  ttttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGACATTGCAAAGGTGTTAACGTTTTTCTAACGGAGTACGGGCGAACTTGATGTACGCGAAAaggagg                                                                           >  1:4254277/1‑140 (MQ=255)
                                                                  ttttCTGTATAATGGGGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCGTGGCAAACGTGTTGAGGTTTTTATCACGGAGAAATGTCGAACTTGATGTAGGCGAACaggatg                                                                           >  1:2373349/1‑138 (MQ=255)
                                                                   tttCTGGATAATGGTGTTTGTTGGTCTTTGACTGCGATGAAGGGAGAGGTTGCTGACACACCCGGCCGCTTTGTCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAggg                                                                          <  2:3599629/140‑1 (MQ=255)
                                                                   tttATGTATAAGGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCAATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAggg                                                                          <  1:2250348/140‑1 (MQ=255)
                                                                     tCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTGCCTGGCCAAGGTGTTGAGGTTTTTATCACGGAGCACTGTCTAAAATGATGGATGCGAAAATGAgggca                                                                        >  1:792660/1‑138 (MQ=255)
                                                                       tGTATAATGGTGTATGTTGTTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTCCAAGGCAAATGTGTTAAGGTTTTTATAACGGATAAATGTCTAACTTGATGTATGCGAAAaggaggtcaaa                                                                      >  1:3241895/1‑135 (MQ=255)
                                                                         tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAAATTGATGTAGGCGAAAAGGAGGGAAaata                                                                    >  2:4888946/1‑140 (MQ=255)
                                                                         tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACAGTCTAACTTGATGTAGGCGAAAAGGGGGGAAaata                                                                    >  1:3699564/1‑140 (MQ=255)
                                                                              tGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATTGCAAATGTGTTAAGGTTTTTATCACGGAGAACTGTCTAAATTGATTTAGGCGAAAACGAGGGAAAATAAAGGc                                                               >  1:3591161/1‑140 (MQ=255)
                                                                              tGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGc                                                               <  1:1988176/140‑1 (MQ=255)
                                                                              tGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTAACCGAGAACTGTCTAACATGAAGTAGGCGAAAAGGAGGGCAAATAATGGc                                                               >  1:2738768/1‑140 (MQ=255)
                                                                                gtgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaa                                                             <  2:3378453/140‑1 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGGGTTGAGGTTTTTATAACGGAGAACAGTGTAACATGATGTAGGGGAAAATGAGGGAAAAAAATGGcaaaac                                                          >  1:741255/1‑140 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCCAAGGTGTTGAGGTTTTTATGACCGAGTACTGTCTAACTTGATGTAGGCGCAAATGAGGGAAAAAAATGGcaaaaa                                                          >  1:886703/1‑139 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCAAGGTGTTCAGGTTTTTATAACCGGGAAATGTATAACTTTATGTAGGCGAAAAGGAGGGAAAATAATTGcaaaaa                                                          >  1:1022084/1‑139 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTGTCACGGAGAAAAGTCTAACATGATGTAGGCGAAAAGGAGGGAAAAAAATTGcaaaac                                                          >  1:134665/1‑140 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGCGAACTGTCTAACTTTATGTAGGCGAAAACGAGGGAAAATAAAGGcaaaac                                                          >  1:4159704/1‑140 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaaac                                                          >  2:2177863/1‑140 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaaac                                                          >  2:2439076/1‑140 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACGGTCTAACTTGCTGTAGGCGAACAGGAGGGAAAAAAATGGcaaaac                                                          >  2:1713986/1‑140 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACATGATGTATGGGAAAAGGAGGGCAAAAAATGGcaaaac                                                          >  1:4745270/1‑140 (MQ=255)
                                                                                   tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAACGTGTTAAGGTTTTTATCACGGAGAAATTTCTAAAGTGATGTAGGGGAAAATGAGGGAAAATAATTTcaaaaa                                                          >  1:1068178/1‑139 (MQ=255)
                                                                                        tGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCAAGGCAAAGGTGTTGAGGTTTTTGTAAAGGAGAAATTTATAACTTTATGTATGCGAAAAAGAGGGAAAATAATGGCAAAACaaaaa                                                     >  1:2579471/1‑140 (MQ=255)
                                                                                          gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCAAAGGTGTTCAGGTTTTTATCACGGCGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAAAGGCAAAACaaaaaaa                                                   >  1:2892575/1‑139 (MQ=255)
                                                                                          gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCATGCAAAGGTGTTGAGGTTTTTCTAACGGCTAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATTGCAAAACaaaaaaa                                                   >  1:4793377/1‑139 (MQ=255)
                                                                                          gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCa                                                                                                                                     <  1:3355565/58‑1 (MQ=255)
                                                                                          gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAATGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAAAGTGATTGAGGCGAAAACGAGGGAAAAAAAAGGCAaaaaaaaaaaa                                                   >  1:3862608/1‑139 (MQ=255)
                                                                                          gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAAGGCAAAGGTGTTGAGGGTTTTGTCACGGAGAACAGTCTAACTTGAGTTAGGGGAAAAGGAGGGAAAATAATGGCAAAAAAAAAAat                                                   >  1:3417342/1‑140 (MQ=255)
                                                                                          gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGCCATTGCAAACGTGTTCAGGTTTTTATGAACGAGAACAGACTAACTTGTTGTAGGCGAAAAAGAGGGACAATAATGGCAaaaaaaaaaaa                                                   >  1:4582050/1‑139 (MQ=255)
                                                                                           tCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCAAAAGTGTTCAGGTTTTTCTGACCGCGAAATGTCTAACAGTAAGTAATAGAAAAGCAGGGAAAAAAAAGGCAAAACaaaaaaat                                                  >  1:1498921/1‑138 (MQ=255)
                                                                                           tCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCAGACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAatt                                                  >  2:3905045/1‑140 (MQ=255)
                                                                                           tCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTGTAAAGGAGAAAAGTCTAAATTGATTTAGGCGAAAATGAGGGAAAATAATGGCAaaaaaaaaaaat                                                  >  1:1458466/1‑138 (MQ=255)
                                                                                             tttGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattcg                                                >  2:4264458/1‑140 (MQ=255)
                                                                                             tttGACTGCGAGGAAGGGAGAGGTTGCTGACACACCCGGCCGCTTTGGCATGGCAAAGGTGTTCCGGTTTTTATCACTGCGTACTGGCTGAACTGGTGGAGGCGACAAGGAGGGAAAATAAATGCAAAACACAAAaatcg                                                >  1:1162871/1‑140 (MQ=255)
                                                                                               tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTACAGGGGAAATGTGTTGATGTTTTTATAACGGAGAAAACTATAAAATGATGTATGCGAAAAGGTCTGAAAAAAAAGGCAaaaaaaaaaaatacta                                              >  1:3332047/1‑134 (MQ=255)
                                                                                                 tcTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACTGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattcgtatt                                            <  2:1312760/139‑1 (MQ=255)
                                                                                                 tcTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattcgtatt                                            <  1:676244/139‑1 (MQ=255)
                                                                                                     cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGCGAACTGTCTCACTTGATGTGGGCGCACCGGAGGGAAAATAAAGGCCAAAGACAAAaatcgtagtcgag                                        >  2:2977091/1‑138 (MQ=255)
                                                                                                     cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAAATTGATTTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGtt                                        >  2:2389985/1‑140 (MQ=255)
                                                                                                        tGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGa                                     <  1:3961125/140‑1 (MQ=255)
                                                                                                        tGAAGAGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGa                                     <  2:3747211/140‑1 (MQ=255)
                                                                                                               agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                              <  1:1670257/140‑1 (MQ=255)
                                                                                                                  ggTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGa                           <  2:4986625/140‑1 (MQ=255)
                                                                                                                  ggTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGa                           <  2:163357/140‑1 (MQ=255)
                                                                                                                  ggTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCACGAATTTCTCACGGAGAACTGTCTAACTAGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATACCTAATCGATTGAAAGCATATGa                           <  1:2089927/140‑1 (MQ=255)
                                                                                                                  ggTGGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGa                           <  2:4047673/140‑1 (MQ=255)
                                                                                                                      gCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCAt                       >  2:4471756/1‑140 (MQ=255)
                                                                                                                      gCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCAt                       >  2:1436089/1‑140 (MQ=255)
                                                                                                                      gCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAAATCGTATTCGTTTGAAAGCATATGATCaa                       >  2:2813040/1‑139 (MQ=255)
                                                                                                                      gCTGACACACCCGGCCGCGTTTCCCGGGCACAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACGTGTTTTAGGCGAAAAGGGGGGGAAATAATGGCAAAAAAAAAAATTGGTATTCGTTTGAAACAatattttaat                       >  1:3080766/1‑134 (MQ=255)
                                                                                                                       atGACACACCCGGCCGCTTTGCCATGGCAAATGTGTTCAGGTTTTTCTCACGGATAACTGTCTAAATTGGTGTAGGCGAAAAGGAGGGAAAATAAAGGCAAAACAAAAAATTCGTCTTAGTTTGAAATCATAAGTTCATa                      >  2:4168650/2‑140 (MQ=255)
                                                                                                                        tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  1:2410719/140‑1 (MQ=255)
                                                                                                                              acCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGCAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCt               >  2:1596940/1‑140 (MQ=255)
                                                                                                                              acCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTGTAACTTGATGTAGGCGCAAAGGAGGGAAAATAATGGCAAAAAAAAAAAATCGTATTAGTTTGAAAGCATAATTTCATAAAATACt               >  2:2077981/1‑140 (MQ=255)
                                                                                                                                ccGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGCGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAAAAATGGCAAAAAAAAAAATTCGTATTCGTTTTTAACCATATGATCATAGAATCCTTg             >  2:1773566/1‑140 (MQ=255)
                                                                                                                                 cGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTTATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGa            <  2:3298971/140‑1 (MQ=255)
                                                                                                                                   gCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATc          <  2:4753762/140‑1 (MQ=255)
                                                                                                                                   gCCGCTTTGACATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATc          <  2:5123860/140‑1 (MQ=255)
                                                                                                                                    ccGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCa         <  2:4113491/140‑1 (MQ=255)
                                                                                                                                    ccGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGAACa         <  2:2485171/140‑1 (MQ=255)
                                                                                                                                     ctcTTTGCCATGGAAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAAt       <  2:2930168/138‑1 (MQ=255)
                                                                                                                                     cGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCaa        <  1:3999900/140‑1 (MQ=255)
                                                                                                                                      gCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAAt       <  1:1728907/140‑1 (MQ=255)
                                                                                                                                      gCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAAt       <  2:2654300/140‑1 (MQ=255)
                                                                                                                                       gcgttGCCATGGCAAAGGTGTTCAGGTTTTTCGCACGGAGAACTGTCTTACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGCTTGAAAGCATATGATCATAGAATCCTTGATCAAt       <  2:1101112/137‑1 (MQ=255)
                                                                                                                                         tttGGCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGACCTGGATAACTTGATGTAGGCGAAAAGGAGGGCAAATAATGGCAATACAAAAAATTCGGAGTGGTTTGAAATCAGAAGATCATAGAATCCAAGATCAATCt     >  2:671588/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CACCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCA  >  NZ_CP034943/134978‑135256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: