Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,769,576 T→G 7.6% I281S (ATT→AGT)  sacX → SacY negative regulator SacX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,769,5760TG 7.6% 867.8 / 14.2 341I281S (ATT→AGT) sacXSacY negative regulator SacX
Reads supporting (aligned to +/- strand):  ref base T (85/216);  new base G (37/3);  total (122/219)
Fisher's exact test for biased strand distribution p-value = 1.96e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGC  >  NZ_CP034943/3769437‑3769712
                                                                                                                                           |                                                                                                                                        
aaGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCGTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAt                                                                                                                                          >  1:2082119/1‑140 (MQ=255)
aaGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAt                                                                                                                                          >  2:3661004/1‑140 (MQ=255)
   cTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                       <  2:5051348/140‑1 (MQ=255)
    tATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTc                                                                                                                                      <  2:2514641/140‑1 (MQ=255)
     aTTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCt                                                                                                                                     <  1:3411455/140‑1 (MQ=255)
      ttAAAATCAATTGTGCCGACGTCGCTTGATGTCGTCATCATTCCTGTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATCTCACAGTCGATTGTCtg                                                                                                                                    >  2:3997986/1‑139 (MQ=255)
      ttAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCta                                                                                                                                    <  2:3092455/140‑1 (MQ=255)
      ttAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCta                                                                                                                                    <  2:4379460/140‑1 (MQ=255)
              aaTTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTAGATTTAtt                                                                                                                            >  2:3418140/1‑139 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTTTGAt                                                                                                                          >  1:2694922/1‑140 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATGACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCAGCTATCATCGGCCAAATTATGACACAGTGGATTGTAGATATTTATgtt                                                                                                                          >  2:1087579/1‑138 (MQ=255)
                     ccGAAGTCGCGTGATGTCGTCATCATTCCTTTTCTTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGc                                                                                                                     <  1:4194576/140‑1 (MQ=255)
                      cGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGcc                                                                                                                    <  1:4422446/140‑1 (MQ=255)
                        aaGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGGGTATATTTATGATCACGCCgg                                                                                                                  >  2:4500602/1‑140 (MQ=255)
                        aaGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCGATATTTATGATCACGCCgg                                                                                                                  >  2:1533807/1‑140 (MQ=255)
                             gCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttg                                                                                                             >  2:1015358/1‑140 (MQ=255)
                              cTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgt                                                                                                            <  1:1554317/140‑1 (MQ=255)
                              cTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgt                                                                                                            <  1:1316932/140‑1 (MQ=255)
                              cTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgt                                                                                                            <  1:710202/140‑1 (MQ=255)
                                tGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgttg                                                                                                          <  2:1959223/140‑1 (MQ=255)
                                   tGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                       <  2:892694/140‑1 (MQ=255)
                                   tGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                       <  2:791686/140‑1 (MQ=255)
                                   tGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                       <  1:3173005/140‑1 (MQ=255)
                                   tGTCGTCATCATTCCTATGATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCGATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                       <  1:2997971/140‑1 (MQ=255)
                                   agtcgtcATCATTCCTTTTCTTCCCGTCATGGTTACAGGGTGCCGCGCGCTGATTGTGATGACTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTGGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                       <  1:4583971/139‑1 (MQ=255)
                                       gtcATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGc                                                                                                   >  1:4717505/1‑140 (MQ=255)
                                       gtcACCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCAAGCCGGCATTGTTGCCGGAGc                                                                                                   >  1:1441256/1‑140 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  2:2085221/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  1:4799271/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  2:4264535/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  2:1821479/140‑1 (MQ=255)
                                               tCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCt                                                                                                                                     <  1:4094306/98‑1 (MQ=255)
                                               tCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCt                                                                                                                                     >  2:4094306/1‑98 (MQ=255)
                                                 cTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                         <  2:3371193/140‑1 (MQ=255)
                                                 cTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                         <  2:3575354/140‑1 (MQ=255)
                                                 cTTTTATTACGCTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                         <  2:3033158/140‑1 (MQ=255)
                                                   tttATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTAAAATTATGATAACGCCGGCAGTGTTGCCCGAGCCCGGTTcggccg                                                                                       >  1:3724298/1‑138 (MQ=255)
                                                    ttatgaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGCATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                      <  1:793384/140‑1 (MQ=255)
                                                       ttaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATc                                                                                   <  1:2817054/140‑1 (MQ=255)
                                                       ttaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATc                                                                                   <  1:4373544/140‑1 (MQ=255)
                                                       ttaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATc                                                                                   <  2:3284390/140‑1 (MQ=255)
                                                       ttaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATc                                                                                   <  1:377410/140‑1 (MQ=255)
                                                       ttaCGGTCATGGTGACAGGGTGCCTCGCGATGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAACTTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATc                                                                                   <  2:3940410/140‑1 (MQ=255)
                                                         aCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTa                                                                                 <  1:2588784/140‑1 (MQ=255)
                                                         aCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTa                                                                                 <  1:309162/140‑1 (MQ=255)
                                                         aCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATAATTATTATCACCCCGGCATTGTTTCCGGCGCCCTGTTCGGCGGCCTCTa                                                                                 >  1:3302708/1‑140 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                              <  1:5070402/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTGTATATTTATTATCACGCCGGCATTGTTGCCGGAGCCCGGTTAGGCGGCATCTaata                                                                              >  2:2003033/1‑137 (MQ=255)
                                                            gTCATGGTGACAGCGTGCCTCGCGCTGATTGAGATGAATCCCGCCTCATCTATAATCGGCCAAATTATGACACAGTCGATGGTCGAGATTTATGATAACGCCCGCAATGTTGGCCGAGCCCTGGTAGGCGGCATCTAATc                                                                              >  2:2849643/1‑140 (MQ=255)
                                                               aTGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGGCGATATTTAAGATCACGCCCGCAGTGTTTCCGGCCCCCGGTTTCGCGGCATAGAcaccac                                                                           >  1:1774241/1‑140 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGCTGGTGATGAATCCCGCCGCACCTATCATCGCCCACCGTATGCCACAGTCCATGGTCTATCTTAATGATCACGCCGGCCTTCTTGCCGGAGCCCTGTTCGGCGGCCTCTACTccaccc                                                                         <  2:2632447/140‑2 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  2:4717505/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  1:3661004/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  2:1563416/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACACTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  1:447949/140‑1 (MQ=255)
                                                                     aCAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGt                                                                     >  1:4860834/1‑140 (MQ=255)
                                                                      cAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGtt                                                                    <  1:4863469/140‑1 (MQ=255)
                                                                       atcgTGCCTCGCGATTATTGTGATTAATACAGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTTTATATATTTATTATAAACCCGGAAATTTTTACCGAGCCCTTTTCGGAGGAATATACTAAACCATAGtta                                                                   >  2:4826723/4‑139 (MQ=255)
                                                                           tGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATTATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACGCCACCATCGTTCTATc                                                               >  2:2983999/1‑140 (MQ=255)
                                                                             ccTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                          >  2:1641290/1‑79 (MQ=255)
                                                                             ccTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                          >  2:1216858/1‑79 (MQ=255)
                                                                             ccTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                          <  1:1641290/79‑1 (MQ=255)
                                                                                cgcgCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                          <  1:1030831/140‑1 (MQ=255)
                                                                                 gcgcTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCtt                                                         <  2:3544848/140‑1 (MQ=255)
                                                                                 gcgcTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATAAAGCCGGCATTGTTGCCGGAGCCCGGTTGGGCGGCATATACTCCCCCATAGTTCTATCCGGCtt                                                         >  2:568933/1‑140 (MQ=255)
                                                                                   gcTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCCTTGTTGCCGGAGCCCTGTTCGGCGGCCTCTACTCCACCCTCGTTCTATCCGGCGTGc                                                       >  2:1376364/1‑140 (MQ=255)
                                                                                      gATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatc                                                    <  1:2538269/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                      <  1:5178389/105‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                   <  1:2240085/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                   <  2:1947866/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                   <  1:3916143/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCGTTATATCCGGCGTGcatca                                                   >  2:1150556/1‑140 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTATATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                   <  2:4174247/140‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGTCCAAATTAGGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                         <  2:4696467/140‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                         <  2:2837778/140‑1 (MQ=255)
                                                                                                 aTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTTCATCATAGCTTTTAt                                         >  2:5187977/1‑140 (MQ=255)
                                                                                                     cgccgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCGTGCATCATAGCTTTTATTCGa                                     >  2:4522384/1‑140 (MQ=255)
                                                                                                      gccgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGAt                                    <  1:2161819/140‑1 (MQ=255)
                                                                                                            tCTATCATCGGCCAAATTATGACACAGTCGATTTTATATATTTATTATAAAGCCGGCATTGTTGCCGGAGCCCAGTTCGGCGGCATCGACTCCACCATCGTTCGATCCGGCTTGCATCATAGCGTTTTTGCGATTGTAGc                              >  2:3854640/1‑140 (MQ=255)
                                                                                                            tCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTGTTATGCGATTGAAGc                              >  2:2406872/1‑140 (MQ=255)
                                                                                                            tCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCAGTGTTGCCGGAGCCCGGTTCGGCGGCATCTACTCCACCATCGTTCTACCCGGCTTGCATCATAGCTTTTATGCGATTGAAGc                              >  2:2895608/1‑140 (MQ=255)
                                                                                                                tcatcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACg                          <  2:37971/140‑1 (MQ=255)
                                                                                                                tcatcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACg                          <  2:3694401/140‑1 (MQ=255)
                                                                                                                tcatcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACg                          <  2:2684891/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        <  1:3229367/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        <  1:925608/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        <  1:4772642/140‑1 (MQ=255)
                                                                                                                     ggCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCCTTGTTGCCGGAGCCCTGGTCGGCGGCAACTACCCCACCAACGTTCTAAGCCGCTTGCAACAAAGCTGTGATGCGCATGTAGCAACgcggcg                     >  2:2239780/1‑139 (MQ=255)
                                                                                                                       ccAAATTATGACACAGTCGAGTGTGTATATTTATTATCACGCCGGCATTTTTGCCGGAGCCCTGTTCGGCGGCATATAATCCACCATAGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCtgcttg                   >  1:2416505/1‑140 (MQ=255)
                                                                                                                          aaTTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTa                                                                                 <  1:1180311/75‑1 (MQ=255)
                                                                                                                                 acacAGTCGATTGTGTATATTTATGATCACGCCGGCATTTTTTCCGGAGCCCTGTTCCGCGGCATCTACTCCACCATCGTTATATCCGGCTTGCATCATAGCGTTTATGCGATTGAAGCAACGCTGGTTTCTGATAcgcg         >  1:385133/1‑140 (MQ=255)
                                                                                                                                 acacAGTCGATTGGCTATATTTATGTTAACCCCCGCCGTGTTGGCGGAGCCCTGTTCGGCGGCATCTACTACACCATCGGTCTATACGGCTTGGATCATTGCTTTTTTGCGATTGAAGCAACGCTGCTTGCTGATCcggg         >  1:4564618/1‑138 (MQ=255)
                                                                                                                                   acaGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCgtg       <  1:1954480/140‑1 (MQ=255)
                                                                                                                                   acaGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCgtg       <  1:1105601/140‑1 (MQ=255)
                                                                                                                                    caGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        <  1:186738/122‑1 (MQ=255)
                                                                                                                                    caGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        >  2:186738/1‑122 (MQ=255)
                                                                                                                                     aGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                         <  2:4241917/104‑1 (MQ=255)
                                                                                                                                     aGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTAt                                         >  1:4241917/1‑104 (MQ=255)
                                                                                                                                     aGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGGTCTATCCGGCTTTCATCATAGCTTTTATGCGATTGTAGCAACCCTGCTTGCTGATCCGCGTGTc     >  1:3591290/1‑140 (MQ=255)
                                                                                                                                       tCGATTGTCTATATTTATGATCACGCCGGCATTGTTTCCGGAGCCCTGTTCGGCGGCATCTACTCCAACATCGTTGTATACGGCTTTCATCATAGCTTTTTTTCGATTGAAGCAACCCTGCGTGCTGATCCGCGTGTAgg   >  1:4012811/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGC  >  NZ_CP034943/3769437‑3769712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: