BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,230	G→T	5.8%	Q49K (CAG→AAG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,230	0	G	T	5.8%	1016.1 / 5.0	383	Q49K (CAG→AAG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (267/75); new base T (6/34); total (273/110)
Fisher's exact test for biased strand distribution p-value = 3.98e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 384 total aligned reads displayed.

GTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851092‑3851369

gTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGt                                                                                                                                            >  1:3754661/1‑140 (MQ=255)

  ccTGCAATGTCCCAATCTGCTCTATGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTAt                                                                                                                                          <  2:4699600/140‑1 (MQ=255)

  ccTGCAAGGTCCCAAGCGGCTCTCTGTAATATCCCTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTAt                                                                                                                                          <  2:5163780/140‑1 (MQ=255)

   cTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATc                                                                                                                                         >  2:2748877/1‑140 (MQ=255)

    tGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCa                                                                                                                                        <  1:1256020/140‑1 (MQ=255)

         tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                   <  1:1078049/140‑1 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAttag                                                                                                                                 >  2:2914328/1‑138 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                 >  1:842235/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                 >  2:2851061/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                 >  1:4254471/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                 >  1:269278/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                 >  1:2619059/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                 >  1:1619121/1‑140 (MQ=255)

             ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                               <  2:1027604/140‑1 (MQ=255)

              cactCTGCTCTCTGTAATATGGATTGGACATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCACTCGTTATTACCTGTATCATGATTCGATc                                                                                                                              <  1:3350422/137‑1 (MQ=255)

              cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                              <  2:3536284/140‑1 (MQ=255)

              cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                              <  1:3221830/140‑1 (MQ=255)

              cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                              <  1:2889796/140‑1 (MQ=255)

              aaaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                              <  2:4831253/139‑1 (MQ=255)

               aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                             <  2:923011/140‑1 (MQ=255)

               aaTCTGCTCTCTGTAATAGGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                             <  2:1625788/140‑1 (MQ=255)

                aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTTTATAATGATTCGATAGt                                                                                                                            >  2:4316689/1‑140 (MQ=255)

                aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                            >  2:4909082/1‑140 (MQ=255)

                aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                            >  1:3832920/1‑140 (MQ=255)

                aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                            >  2:4211150/1‑140 (MQ=255)

                aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATCATTCTATCGt                                                                                                                            >  1:3512718/1‑140 (MQ=255)

                  aggCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAt                                                                                                                          >  1:2799949/3‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                                       >  1:3665575/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:2390872/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:2537098/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:2728931/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:2277161/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:3092382/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  2:5090730/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:2943079/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:4636471/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCa                                                                                                                       >  1:696131/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACAAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  1:1989436/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAAACAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                       >  2:3400950/1‑140 (MQ=255)

                         ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                   <  1:479159/140‑1 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGACCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                  >  1:3933764/1‑140 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCATTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                  >  2:1276592/1‑140 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                  >  1:742711/1‑140 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                  >  2:4435976/1‑140 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                  >  2:1784443/1‑140 (MQ=255)

                           gTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCt                                                                                                                 >  2:2863928/1‑140 (MQ=255)

                               tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                             >  2:749543/1‑140 (MQ=255)

                               tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                             >  1:357121/1‑140 (MQ=255)

                                  ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCGTCCCaaa                                                                                                          >  2:1924091/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAATCCCTTCCCaaaaa                                                                                                        >  1:4944269/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                        >  1:1344806/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                        >  2:4540619/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                        >  2:2050335/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                        >  1:1916004/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                        >  1:4881078/1‑140 (MQ=255)

                                      tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                      >  1:1426230/1‑140 (MQ=255)

                                      tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTAAGCAAGCCCGTCCCAAAAACg                                                                                                      >  2:4627736/1‑140 (MQ=255)

                                             ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAc                                                                                               >  1:1502686/1‑140 (MQ=255)

                                                                    aTCACGTTGTAAGAATAACATTAAAAGTACTCGGACAGGTGTTAAAAAAATCAACCAGTCGTTTTTACCTGTATATTGTTTCGCTCGTCTGCACGCCTGCCCAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCa                                                                        <  2:3221373/140‑1 (MQ=255)

                                                                          ttGTACGAATAACCTTCAAAGTACTCGTACCGGTATTATATAATTTAACCAGTCGTTATTACCTGTAAATTTATTCTATGGTATGCAACCCGTACCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                  <  2:2354113/140‑1 (MQ=255)

                                                                          ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTATGCTTCTTCAAGTTAGCAAGACAt                                                                  >  2:1369792/1‑140 (MQ=255)

                                                                          ttGTAAGCACAACCTTAATCGTAGTCGGACTGGGAGACTATAAATCACCCTGTCTTTATTACGAGTATCATTATTCGATCGTCGGAACGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                  <  2:4066626/140‑1 (MQ=255)

                                                                                  aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACtt                                                          >  2:3779337/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATACATGAACCAGTCGGTATTACCTGTATCATGATTCGATCGTATGCCAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATACCAACTtg                                                         >  2:1433535/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACCACTCTGCTTCTTCAAGTTCGCAAGACAAAACAAATtg                                                         >  1:4482206/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACttt                                                         >  2:2415632/1‑139 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  1:681303/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  2:3950117/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  1:1801965/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  2:4583841/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCTCAAGACATAACAACTtg                                                         >  2:1237720/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTGCTATAAATGAACCAGTCGTTATTACCTGTATCAGGTTTAGAGCGTATGCAAGCCCTTCCCAAATAGGATAAAACTAGTCTGCTTCTTCAAGTTCGCTAGTCATAACAACTtg                                                         >  2:2556418/1‑140 (MQ=255)

                                                                                    aaCCTACAAATTACTCGCACAGGTCTTATAAAAAGCAACCTGTTTTTATTCCCTTTATCATTATTCGAGCGTCTCCACCCCATACCCAAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                        <  1:3427941/140‑1 (MQ=255)

                                                                                      ccTTCATTGTACCCGTACAGGTCTTCTATAAAGAAACCAGCGTTTATTCCCTGTATCATTCTTCGATCGTATCAAAGCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:329179/140‑1 (MQ=255)

                                                                                      ccTTCAAAGTACTGGTACTGTTCTTATATAAATTAACCATTCGTTATTACCTGTATCATGATTCGATCGTTTCAAAGCCTTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:529549/140‑1 (MQ=255)

                                                                                      ccTGCAACGTCCTCGGAGAGGTCTTATATCAATGACCCAGTCTTTATTACGTTTAGCTTGATACGATCTTCTGCACCCCCTGCCACAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:3074811/140‑1 (MQ=255)

                                                                                      acTTAAAATTACTGGCACAGGATTTATATAAATGACCCAGTCTTTATTACATTTATCATTATTCGATCGTATGCAAGCCCTCCCCAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:1098241/139‑1 (MQ=255)

                                                                                      acTTAAAATTACCCGGACAGGATTTCTAAAACTGAACCAGTCTTTATTACCTTTACCATGATTCGATCTTATGCACGCCCTCCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:3661439/139‑1 (MQ=255)

                                                                                           aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCACGCCCTTCCCAAAAACGATAAAACGACACTGCGTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCt                                                 >  2:2176253/1‑140 (MQ=255)

                                                                                                 cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAAAGATAAAACGACTATGCTTCTTCAAGTTAGCAAGACATAACAAATTGATGAGGCTTAagag                                           >  1:799011/1‑140 (MQ=255)

                                                                                                 cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                           >  2:803627/1‑140 (MQ=255)

                                                                                                 cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                           >  1:1048886/1‑140 (MQ=255)

                                                                                                 cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATGATTCGAACGTAAGCAAGCCCTTCCCAAAAACGATAAAAACACTATGCTTCTTCAATTTAGCAAGACAAAACAACTTGATGAGGCTAAagag                                           >  1:2243321/1‑140 (MQ=255)

                                                                                                     tagtgttcttttATAAATTACCCATTCTTTATTACTTGTATAATGTTTCTATGGTTGGCACCCCCTCCCCAAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                       <  1:4185815/134‑1 (MQ=255)

                                                                                                     gACCGGTATTATATAAATCAACCATGCGTTTTTACGTTTATCATTTTTCGTTCGTATCCAACCCCTACCAAAAACCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                       <  1:1182406/140‑1 (MQ=255)

                                                                                                         ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                   >  2:1568265/1‑140 (MQ=255)

                                                                                                         ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                   >  2:1966341/1‑140 (MQ=255)

                                                                                                              tatataAATCAACCATTGTTTTTTACCTGTATCTTGTTTCGATCGTATGAAAGCCCTTCCAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                              <  2:696131/138‑1 (MQ=255)

                                                                                                                   aaaTTAACCCGTCTTTATTACCTTTACCATTATGCCATCGTCTCAAACCCCTACCAAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                         <  1:3169668/140‑1 (MQ=255)

                                                                                                                    aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                        >  1:5144625/1‑140 (MQ=255)

                                                                                                                    aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGAGCGATAATGTCTAAAtctgt                        >  1:4360103/1‑136 (MQ=255)

                                                                                                                     aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGCTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                       >  1:994990/1‑140 (MQ=255)

                                                                                                                     aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTTATGAGGCTTAATAGCGATCATTTCTAAATTTCCg                       >  1:409900/1‑140 (MQ=255)

                                                                                                                     aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                       >  1:5047012/1‑140 (MQ=255)

                                                                                                                     aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAACCCCTTCCCAAAAACGATAAAACGACTATGCTTCTTCAACTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGGCg                       >  1:171284/1‑140 (MQ=255)

                                                                                                                     aTGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTGTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTTCCg                       >  1:2086845/1‑140 (MQ=255)

                                                                                                                        aaCCTGTCGTTATTACCTTTATCATGATTCGATCGTCTGCAAGCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:2469403/140‑1 (MQ=255)

                                                                                                                         aCCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATaa                   >  1:917582/1‑139 (MQ=255)

                                                                                                                                      aCCTGTATAATGATTCGATCGTATGCAAGCCATTCCCAAAAAAGATAAAACGACAATTCTTCTTCAAGTTCGCAAGACATAACAACTTGTTGAGGATTAAGATCGATCATGTCTAAATTGCCGATACTGCTTATATCGGt      >  2:4751311/1‑140 (MQ=255)

                                                                                                                                          gTATCTTGATCCTAACGTATCCACCACATTCCAAAAACCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATCTCTAAATTGCCGATACTGCTTATAGCGGTTAtt  <  1:2070031/140‑1 (MQ=255)

GTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851092‑3851369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`