Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,851 A→T 10.6% intergenic (‑157/+49) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8510AT10.6% 805.9 / 20.9 271intergenic (‑157/+49)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base A (129/107);  new base T (2/32);  total (132/139)
Fisher's exact test for biased strand distribution p-value = 1.68e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

AACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGG  >  NZ_CP034943/3996712‑3996990
                                                                                                                                           |                                                                                                                                           
atcatttCAAAACGATTCTATTCAAACATATCAAGTTTATCATTGAAGATACAGAAAGTCATTTTTAAATCGGAACAGTAAATTATTGTAAATCATTATTCGTTATCTTCCTTTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  2:5084473/136‑1 (MQ=255)
acccTTTCAACACGATTCGAGTCAAACACATCAAGGTGATCAGTGAAGACACAGAAAGTCAATTGTAAATCCGAACATGAAATTACTGTAAATCAATATTCGTTCGCTACCATGCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  2:2654726/138‑1 (MQ=255)
aaacTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  2:4095065/137‑1 (MQ=255)
aaCCTTTCACAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:2073851/140‑1 (MQ=255)
aaCCTTTCAAAACTATTCTAGTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  2:2441793/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACACATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:1230915/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAACTCAAGTTTATCAGTTAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAAGATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  2:1483433/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:2755715/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  2:2559830/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:2866753/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:3180421/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:1429180/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:4352430/140‑1 (MQ=255)
aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  2:775673/140‑1 (MQ=255)
aaCCTTTCAAAACGATTAGATTCACACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTACCTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:2335818/140‑1 (MQ=255)
aaCCTGTCACAAAGATTCGATTCAAACAAATCAAGTGTATCAGGGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             <  1:1901918/140‑1 (MQ=255)
     ttCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCTAACATTAAATTAATGTAAATCAATATTCGTTCTCTGCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTc                                                                                                                                        >  1:1396992/1‑140 (MQ=255)
     ttCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTc                                                                                                                                        >  1:4481792/1‑140 (MQ=255)
     ttCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTc                                                                                                                                        >  1:2512698/1‑140 (MQ=255)
     ttCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTc                                                                                                                                        >  2:2305072/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTTGACATCCGAACCTTAAATTAATGTAAATGAATATTCGTTCGGTTGCCTTAGTTTTACATGCGTTTTCTCCTGGCATTGTcc                                                                                                                                       >  1:4194820/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                             >  2:1382762/1‑134 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                       >  2:33603/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                       >  1:5025781/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTATCATCGCATTGTcc                                                                                                                                       >  2:2776341/1‑140 (MQ=255)
      tCAAAACGATTCTATTCAAACAAAGCAAGTTTATCAGGGAAGAAACAGAAAGTCAATTGTAAATCCGACCAGTAATTTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                       >  1:1333486/1‑140 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAATGCTCGTGa                                                                                                                         >  2:3639495/1‑140 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                         >  1:940859/1‑140 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                         <  1:4385133/140‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                         <  1:4874359/140‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                         <  1:3605798/140‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                         <  1:961518/140‑1 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                         >  2:2154539/1‑140 (MQ=255)
                    ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGGTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                         <  1:1776595/140‑1 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGACCATTATATTAATGTAAATCAAGATTCGTTCGCTTCCATGCGTTTTTCAAGCGTTTTGTCCTCGCATTGTCCTAAAAAGCTCGGGAAACtt                                                                                                                    >  1:776614/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAATTTATTGTAAATCTATATTCGTTCGCTTCCATTCGTTTTATAAGCGTTTTCTCACTGCTTTGTCATAAAAAGCTTGCGAAACtt                                                                                                                    >  2:865718/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAATTTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                    >  1:1919993/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                    >  2:1668921/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                    >  2:1777015/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                    >  1:4732516/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                    >  2:4304768/1‑140 (MQ=255)
                         acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                    >  1:569704/1‑140 (MQ=255)
                              tCAAGTTTATCAGTGAAGAAACAGAAAGTCACTTGTACATCCGAACATGAAATTAATGTACATCAATATTCGTTCGCTTCCCTTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                               <  2:4949219/140‑1 (MQ=255)
                                      aTCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCa                                                                                                       <  1:4263671/140‑1 (MQ=255)
                                       tctgTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCAc                                                                                                      >  1:3366118/4‑140 (MQ=255)
                                           tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                  <  1:3464451/140‑1 (MQ=255)
                                            gaagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaa                                                                                                 >  2:2865282/1‑140 (MQ=255)
                                             aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCTTGAAAATTTTATCCTTTAGCACGaaaaa                                                                                                >  2:3036711/1‑140 (MQ=255)
                                             aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                >  1:1291842/1‑140 (MQ=255)
                                             aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTGTAGCACGaaaag                                                                                                >  2:3584783/1‑139 (MQ=255)
                                             aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTAGTTCGCTTCCATTCGTTTTAGAAGCGATTTCTCAACGCATTGTCCTAAAAAGATCGTGAAACTTTTATCCTTTCGCACGataaa                                                                                                >  2:3891909/1‑140 (MQ=255)
                                              agaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAAAGTAAATCACTATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAag                                                                                               <  2:2719534/140‑1 (MQ=255)
                                                    aGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAc                                                                                         <  2:374047/140‑1 (MQ=255)
                                                             aTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGat                                                                                <  2:1179953/140‑1 (MQ=255)
                                                             aTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGat                                                                                <  1:3113801/140‑1 (MQ=255)
                                                               tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                              <  2:4030934/140‑1 (MQ=255)
                                                                    aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCTCGAAAAAGAGTTACTTGCCTGATATCCCtg                                                                         >  2:5152207/1‑139 (MQ=255)
                                                                    aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                         >  1:2965922/1‑140 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                    >  1:1552648/1‑140 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGCACGAAATAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                    >  1:4036193/1‑140 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                    <  1:2620245/140‑1 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                    <  2:2333865/140‑1 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                    <  1:2456604/140‑1 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCATTTAGCACGaa                                                                                                   >  2:4141675/1‑109 (MQ=255)
                                                                         aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCAATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaa                                                                                                   <  1:4141675/109‑1 (MQ=255)
                                                                              taaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                 <  1:3558441/90‑1 (MQ=255)
                                                                                    attttAAATCAATTTTGGTTCGTTTCATTTCGTTTTACATGCGTTTCATCTGCTCATTTTGCTAAAATGCTCGTGAAACTTTTATCCTTTAGCACCAAAAAGAGTTACTTGCCTTATATCCTTTTTTTATTAATAAAAAc                                                         <  2:3521734/136‑1 (MQ=255)
                                                                                      tttAATTCATTTTTCTTTCTGTTCCATTTTTTTTAAAAGGGTTTTATCATCGATTTGTCCTAAAAAGCTGGTGAAACTTTTATCCTTTAGCACGAAAAAGTTTTAGTTGCCTGATATCCATTTTTTATTCATAAAAACGa                                                       <  2:5223940/138‑1 (MQ=255)
                                                                                      ttcaaataaatTTTCTTTCGCTTCCATTCTTTTAACATGCTTTTTCTAATGGCTGTGTCAAAAAAAGCTGGTTAAAATTTTATCCTTTAGCACTAAAAATATTTAGTTGCCTGTTATCCCTTTTGTATTCATAAAAACGa                                                       <  2:4198863/137‑1 (MQ=255)
                                                                                      ttaaaataaatTTTCGTTCGCTTACATTCTTTTTACAAGCGTTTTCTCATGGCTTTGTCATAAAAAGCTGGTTAAAATTTTATCCTTTAGAACTAAAATTTTTTACTTGCATGATATCCGTTTTGTTTTAAAAAACACGa                                                       <  2:560696/137‑1 (MQ=255)
                                                                                      tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                       >  2:3232459/1‑140 (MQ=255)
                                                                                      tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                       >  1:1132186/1‑140 (MQ=255)
                                                                                      tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                       >  1:3665295/1‑140 (MQ=255)
                                                                                      tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                       >  2:2048225/1‑140 (MQ=255)
                                                                                           aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                  >  1:871020/1‑140 (MQ=255)
                                                                                              aaTATTCGTTCGCTTCCAGACGTTTTACAAGCTTTTTCTCATCGCATTGTACTAAAAAGCTCTTGAAACTTTTAACCTTTACCACCAAAAACAGTTACTTGCATCATATCCCTTTTGTATTCATAAAAACGACTCGCCaa                                               <  2:1931209/140‑1 (MQ=255)
                                                                                                tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                                             >  2:576771/1‑140 (MQ=255)
                                                                                                           ttCCATTCGTTTTACATTCTTTTTCTCATCGCATTGTCCTAAAAAGGTCGTTAAAATTTTATCCTTTAGCACGAAAAATAGTTACTTGCCTGATATCCGTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCct                                  <  2:1040641/140‑1 (MQ=255)
                                                                                                                  cGTTTTACAAGGTTTTTACCAGCGCTTGTTCATAAAACGAACTTCAAAATTTTATCCTTAACCACAAAAAATATTTACTTGCCTGATATCCTTTTTGTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                           <  1:2273131/140‑1 (MQ=255)
                                                                                                                    tttaACAAGCTTTTTCTAATCGCTTTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCATTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                         <  2:3383413/140‑1 (MQ=255)
                                                                                                                      ttACATGCTTTTTCTCATCGCATTGTCCAAAAATGCTCTTCAAACTTTTATCCTTTACCACTAAAAATTGTTAGTTGCGTTATATCCCTTTTTTATAAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                       <  1:3749700/140‑1 (MQ=255)
                                                                                                                       tACAAGCGTTTTCTCATCTCATTGTCCTAAAAAGCTCGTGAAATTTTTATCCTTTAGCACGAAAAAGTGTTAGTGGCGTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                      <  1:4923793/140‑1 (MQ=255)
                                                                                                                       tACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCTCCAACCGTCCGGATCCTCTATCGTCACGc                      >  2:4390464/1‑140 (MQ=255)
                                                                                                                       tACAAGCGTTTTCACCTCGCATTGTCATAAAAAGCTCGGCAAACTTTTATCCTTTCCCACTAAAAAGTGTAACTTGCCGCATATCCCTTTTTTATCCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                      <  1:3977168/140‑1 (MQ=255)
                                                                                                                        acacgggTTTTCTCATCGATTTGTCCTAAAAAGGTCTTTAAATTTTTATCCTTAACCACCAAAAATAGTTAGTTGCCTTATAGCCCTTTTTTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGcc                     <  1:1658539/134‑1 (MQ=255)
                                                                                                                           aGCTTTTTCTCATCGAATTGTCCAAAAACGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTcc                  <  2:4996170/140‑1 (MQ=255)
                                                                                                                            ggtttttCTCATCGCTTTGTCAAAAAACGCTCGTTAAACTTTTATCCTTTAGCACCAAAAAGAGTTAGTTGCCTGATATCCATTTTTTATAAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                 <  1:198405/137‑1 (MQ=255)
                                                                                                                            gctttttCTCATCGCTTTTTCATAAAAAGATCTTTAAATTTTTATCCTTTAGCACTAAAATGAGTTATTTGCCTGATATCCCTTTTTTTTTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                 <  1:3475664/137‑1 (MQ=255)
                                                                                                                            gctttttCTAATCGATTTGTCATAAAACGGTCGTGAAACTTTTATCCTTTCGCACTAAAAAGATTTACTTGCGTGTTATCCCTTTTTTATAAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                 <  1:2368560/137‑1 (MQ=255)
                                                                                                                            gCGTTTTATAATCTCTTTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACTAAAAATAGTTACTTGCCTTATATCCATTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                 <  2:1542115/140‑1 (MQ=255)
                                                                                                                             ctttttCACATCGATTTGTACAAAAAAGCTCGTAAAAGTTTTATCCTTTAGCACCAAAAAGAGTAACTTGCCTGACATCCCTTTTGTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                <  1:694079/138‑1 (MQ=255)
                                                                                                                                tttCTCATCGCATGTTCCTAAAAAGCTCGTTAAACTTTTATCCTTGCGCACAAAAAAGAGTTAATTGCCTCATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc             <  1:4774739/140‑1 (MQ=255)
                                                                                                                                tttCTCAGCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTGAGCACCAAAAAGAGTTCCTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc             <  2:158586/140‑1 (MQ=255)
                                                                                                                                tttATCACCGCAGTGTCCTAAAAAGCTCGTCAAACTTTTATCCTTTCCCACCAAAACGAGTTACTTGCCTCATATCCCTTTTGTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc             <  1:2899818/140‑1 (MQ=255)
                                                                                                                                 ttCACATCGCATTGTCCAAAACAGCGCGTCAAACTTTTATCCTTTCGCACCAAAAATAGTTAGTTGCCTCATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCt            <  1:3819126/140‑1 (MQ=255)
                                                                                                                                  tctcATCGAATTTTCCTAAAAAGCTGGTTAAACTTTTATCCTTTACCACGAAAAAGAGTTACTTGCCTTATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTc           <  2:3087524/140‑1 (MQ=255)
                                                                                                                                       tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa      >  2:4475031/1‑140 (MQ=255)
                                                                                                                                       tCGCATTGTCCAAAAACGCTCTTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa      <  2:4796551/140‑1 (MQ=255)
                                                                                                                                       tCGCATGGGCCTAAAAACCTCGTCAAACTTTTAGCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa      <  2:3459472/140‑1 (MQ=255)
                                                                                                                                        cGCTTTGTCATAAAAAGCTCGTGAAACTTTTAGCCTTTAGCACCAAAAAGAGTTTCTGGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc     <  1:1533254/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGG  >  NZ_CP034943/3996712‑3996990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: