Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,857 C→A 6.6% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8570CA 6.6% 636.9 / 7.3 244intergenic (‑163/+43)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base C (101/118);  new base A (2/22);  total (104/140)
Fisher's exact test for biased strand distribution p-value = 3.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994
                                                                                                                                           |                                                                                                                                          
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  2:2322948/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  2:268958/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  2:2776284/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCGTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  1:136358/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTAGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTGATCGCATTGTcc                                                                                                                                            >  1:4196075/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACAGTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  1:4102481/1‑140 (MQ=255)
       gATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAg                                                                                                                                     >  2:2468479/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAATGCTCGTGa                                                                                                                              >  2:3639495/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  1:4385277/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              >  1:2978629/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              >  2:2154539/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  1:2683137/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  1:3605798/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              >  1:940859/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              >  1:2327640/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              >  1:4974466/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  1:1635651/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              >  1:4816512/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGATTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  1:369493/140‑1 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAATTTATTGTAAATCTATATTCGTTCGCTTCCATTCGTTTTATAAGCGTTTTCTCACTGCTTTGTCATAAAAATCTTGCGAAACtt                                                                                                                         >  2:3063205/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACATGCGTTTTCTCATCGCATGGTCCTCAAAAGCTCGAGAACCtg                                                                                                                         >  1:4374331/1‑139 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                         >  1:569704/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                         >  1:4732516/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTACATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                         >  1:2082682/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                             >  2:78960/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                             <  1:3473318/140‑1 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                             >  1:831139/1‑140 (MQ=255)
                                 tCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCAc                                                                                                           >  1:1413768/1‑140 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                       >  2:1755619/1‑140 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                       >  1:1759338/1‑140 (MQ=255)
                                      gaagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaa                                                                                                      >  2:2887987/1‑140 (MQ=255)
                                      gaagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaa                                                                                                      >  2:2865282/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                     >  1:1291842/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTAGTTCGCTTCCATTCGTTTTAGAAGCGATTTCTCAACGCATTGTCCTAAAAAGATCGTGAAACTTTTATCCTTTCGCACGataaa                                                                                                     >  2:3891909/1‑140 (MQ=255)
                                              aGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAc                                                                                              <  2:761289/140‑1 (MQ=255)
                                              aGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAc                                                                                              <  2:374047/140‑1 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                             >  2:5122671/1‑140 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                            <  2:3362677/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                            <  2:569045/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                            <  2:4988733/140‑1 (MQ=255)
                                                  aGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                          >  1:3459472/1‑140 (MQ=255)
                                                  aGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                          <  1:1913967/140‑1 (MQ=255)
                                                      aaTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                      <  1:1936212/140‑1 (MQ=255)
                                                       aTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGat                                                                                     <  2:1179953/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  2:2344152/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATTTAAATCATTATTCGTTCGCTTCCATTCTTTTTACAAGCGTTTTCTAATCGCATTGTACTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGACTGATATCCCTTTTGTa                                                                        <  2:4363918/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  1:4763924/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  1:2620245/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                        <  1:2456604/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTGTGTa                                                                        >  1:4035714/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCATTTAGCACGaa                                                                                                        >  2:4141675/1‑109 (MQ=255)
                                                                      attaaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTc                                                                     <  2:3962113/140‑1 (MQ=255)
                                                                        taaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                      <  1:3558441/90‑1 (MQ=255)
                                                                        taaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                      >  2:3558441/1‑90 (MQ=255)
                                                                              attttAAATCAATTTTGGTTCGTTTCATTTCGTTTTACATGCGTTTCATCTGCTCATTTTGCTAAAATGCTCGTGAAACTTTTATCCTTTAGCACCAAAAAGAGTTACTTGCCTTATATCCTTTTTTTATTAATAAAAAc                                                             <  2:3521734/136‑1 (MQ=255)
                                                                               aTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACg                                                            >  2:1689537/1‑140 (MQ=255)
                                                                               aTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATACCTTTTGTATTCATAAAAACg                                                            >  2:1395384/1‑140 (MQ=255)
                                                                                tttAAATCAATATTCGTTCGCTTCCATTCTTTTTACAAGGGTTTTCTCTTCGAATTGTCCTAAAATCGTCGTCAAACTTTTAGCCTTTCCCACGAAAAAGAGTTACTTGCCTTATATCCCTTTTGTTTTCATAAAAACGa                                                           <  2:1928353/138‑1 (MQ=255)
                                                                                ttcaaataaatTTTCTTTCGCTTCCATTCTTTTAACATGCTTTTTCTAATGGCTGTGTCAAAAAAAGCTGGTTAAAATTTTATCCTTTAGCACTAAAAATATTTAGTTGCCTGTTATCCCTTTTGTATTCATAAAAACGa                                                           <  2:4198863/137‑1 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  2:3232459/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:1132186/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  2:2048225/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:3373243/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  1:3665295/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCATTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                           >  2:5196363/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCGTAAAAAGCTCGTGAAACTTGTATCCTTTAGCACGAAACAGAGTTACTTGCCGGATATCCCTTTGGTATTCATAAAAACGACTCGc                                                      >  1:3135165/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  1:3204382/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                                      >  1:871020/1‑140 (MQ=255)
                                                                                             acgtccgCTTCCATTCTTTTTAAAAGCTTTTTATATTCGATTTTTCATAAAACGCTCTTGAAAATTTTATCCTTTAGCACTAAAAATAGTAACTTGCGTTATATCCCTTTTTTAATCAAAAAAACGACTCGCCAACCGTc                                              <  1:5061263/139‑1 (MQ=255)
                                                                                              cgttggTTTCTATTCTTTTTAAAAGCTTTTTATCATCGATTTGTCAAAAAAAGATCGTTAAAATTTTATCCTTTACAACTAAAAATTTTTATTTGCTTTATATCCTTTTTTTATTAAAAAAAACGACTCGCCAACCGTcc                                             <  1:1900187/140‑1 (MQ=255)
                                                                                              cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTcc                                             >  1:2886645/1‑140 (MQ=255)
                                                                                              cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTcc                                             >  1:251493/1‑140 (MQ=255)
                                                                                              cgttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTcc                                             >  1:76570/1‑140 (MQ=255)
                                                                                                   gCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAc                                                  <  2:3631267/130‑1 (MQ=255)
                                                                                                     ttcatttCTTTTTACAAGCGTTTTCTCATCGCTGTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTACCACTAAAACTAGTTAGTTGCCTTATTTCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCct                                      <  2:3156176/135‑1 (MQ=255)
                                                                                                     ttCCATTCGTTTTACATTCTTTTTCTCATCGCATTGTCCTAAAAAGGTCGTTAAAATTTTATCCTTTAGCACGAAAAATAGTTACTTGCCTGATATCCGTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCct                                      <  2:1040641/140‑1 (MQ=255)
                                                                                                     ttCCATGCGTTTTACAAGGTTTTACTAACCGCATTGTCCTAAAAAGATGGTGAAACTTTTATCATTTAGCACGAAAAAGAGTTACTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCct                                      <  2:2649392/140‑1 (MQ=255)
                                                                                                       ccATTCGTTTTACAATCGTTTTCTCATCTCATTGTCCTAAAAATCTCGTGAAACTTTTATCCTTTACCCCGAAAAAGAGTTACTGGCGTTTTATCCCTTTTGTTTTCATAAACACGACTCGCCACCCGTCCGGATCctct                                    <  2:1111561/140‑1 (MQ=255)
                                                                                                         aTTCTTTTGACTAGCTTTTTCCTATGGATTTTTCCTAAAAAGCTCTTAAAAGTTTTATACTTTACAATGAAAATTAGTTACTTGACTTTTATCCCTTTTTTTTTCATAAAAACGACTCGCCAACCGTCCGGTTCCTCTAt                                  <  2:5231167/140‑1 (MQ=255)
                                                                                                              tttaACATGCGTTTTCTCATGGCTTTGTCCAACAACCCTGTTAAAATTTTCATCGTTGACCCCAAAAAATTGTTCTTGCCCGGTAAGCCTTTTGTTATAAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                             <  1:2053028/140‑1 (MQ=255)
                                                                                                              tttaACAAGCTTTTTCTAATCGCTTTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCATTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                             <  2:3383413/140‑1 (MQ=255)
                                                                                                                ttACATGTGTTTTCTCATCGATTTTTCCAAAAAAGCTCGTCAAAGTTTTATCCTTTCGCACCAAAAAGTTTTATTTGCCTGATATCCCTTTTGTATACATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                           <  1:4280929/140‑1 (MQ=255)
                                                                                                                 tACATGCTTTTTCTCTGGGCTTTTTCCAAAAAACGTGTTGAAATTTTTATCCTTTCCCACGAAAATTTTTTACTGGCTTTATATCAGTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                          <  1:4923726/140‑1 (MQ=255)
                                                                                                                 tACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCTCCAACCGTCCGGATCCTCTATCGTCACGc                          >  2:4390464/1‑140 (MQ=255)
                                                                                                                 tACAAGCGTTTTCACCTCGCATTGTCATAAAAAGCTCGGCAAACTTTTATCCTTTCCCACTAAAAAGTGTAACTTGCCGCATATCCCTTTTTTATCCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                          <  1:3977168/140‑1 (MQ=255)
                                                                                                                  aCAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGcc                         >  2:5083786/1‑140 (MQ=255)
                                                                                                                      gctttttCTCCACGCATTTTCAAAAAACGCTCGTCAATCTTTTATCCTTAACCACTAAAAAGAGTTAGTGGCCTTATATCCCTTTTGTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                     <  1:4168609/137‑1 (MQ=255)
                                                                                                                      gctttttCTAATCGATTTGTCATAAAACGGTCGTGAAACTTTTATCCTTTCGCACTAAAAAGATTTACTTGCGTGTTATCCCTTTTTTATAAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                     <  1:2368560/137‑1 (MQ=255)
                                                                                                                      gCGTTTTCTATTGGCATTGTCCTAAAAAGCTCGTGAAAATTTTATCTTTTAGCACCAAAAAGAGTTACTTGCCTGTTATCCCTTTTTTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                     <  2:899690/140‑1 (MQ=255)
                                                                                                                      gCGTTTTATAATCTCTTTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTAGCACTAAAAATAGTTACTTGCCTTATATCCATTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                     <  2:1542115/140‑1 (MQ=255)
                                                                                                                       ggTTTTCTCATCGAATTGTCCTAAAAAGCTCGTGAAAGTTTTATCATTTAGACCGAAAAAGTTTTACTTGGCTTTTATCACTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                    <  2:4075598/139‑1 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAATAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACTACTCGCCAACCTTCCGGATCCTATATCGTCACGCCTCCATgg                  >  2:1003572/1‑140 (MQ=255)
                                                                                                                          tttCTCAGCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTGAGCACCAAAAAGAGTTCCTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                 <  2:158586/140‑1 (MQ=255)
                                                                                                                          tttATCACCGCAGTGTCCTAAAAAGCTCGTCAAACTTTTATCCTTTCCCACCAAAACGAGTTACTTGCCTCATATCCCTTTTGTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                 <  1:2899818/140‑1 (MQ=255)
                                                                                                                                 tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa          >  2:4475031/1‑140 (MQ=255)
                                                                                                                                 tCGCATTGTCCAAAAACGCTCTTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATAGCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa          <  2:4796551/140‑1 (MQ=255)
                                                                                                                                 tCGCATGGGCCTAAAAACCTCGTCAAACTTTTAGCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa          <  2:3459472/140‑1 (MQ=255)
                                                                                                                                  cGCTTTGTCATAAAAAGCTCGTGAAACTTTTAGCCTTTAGCACCAAAAAGAGTTTCTGGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc         <  1:1533254/140‑1 (MQ=255)
                                                                                                                                  cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc         >  1:3779531/1‑140 (MQ=255)
                                                                                                                                     aTTGTCCTAAAAAGCTCGTTAAATTTTTATCCTTTCGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACggg      <  2:4884145/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCT  >  NZ_CP034943/3996718‑3996994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: