BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	209,812	G→T	5.3%	A234S (GCA→TCA)	EO946_RS01190 →	alpha/beta hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	209,812	0	G	T	5.3%	1101.8 / 7.7	434	A234S (GCA→TCA)	EO946_RS01190	alpha/beta hydrolase
Reads supporting (aligned to +/- strand): ref base G (208/186); new base T (38/2); total (246/188)
Fisher's exact test for biased strand distribution p-value = 3.64e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 476 total aligned reads displayed.

GTTCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTATGCCATACAAA  >  NZ_CP034943/209673‑209950

gTTCTACCTGTTAACTTCGATTGGTGTTCATAGGTTTATGACTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            <  1:1299150/140‑1 (MQ=255)

gTTCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            <  1:101845/140‑1 (MQ=255)

gTTCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            <  1:5124168/140‑1 (MQ=255)

gTTCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            <  1:2738977/140‑1 (MQ=255)

gTTCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            <  1:2984840/140‑1 (MQ=255)

gTTCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            <  1:3802890/140‑1 (MQ=255)

gTTCCAAATTTTAACTTCGATTGGAGTTCATCGGTTTATGCCTTCCGCTTTATATAATCCTGAGCTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATACATCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            <  1:4087428/140‑1 (MQ=255)

  tCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCa                                                                                                                                          <  1:3437112/140‑1 (MQ=255)

  tCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCa                                                                                                                                          <  1:1981196/140‑1 (MQ=255)

   ccACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTGGTTAACGGGTGCAGAAAAAGAAATCTATAAAGCCAGTAACTTTCAGCAATTTTTTAATGCAg                                                                                                                                         >  2:170649/1‑140 (MQ=255)

    cACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGa                                                                                                                                        <  2:3159456/140‑1 (MQ=255)

      ccTTTTATCTTCGTGTGGTGTTCCTCGGTTTTTGCCTTCCGCTGTATATAATCCGGATGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAATAAATCTATAAATCCATTAACTGTAAGCAATTTTTTAATGCAGata                                                                                                                                      <  2:2159623/140‑1 (MQ=255)

        tGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATg                                                                                                                                    <  2:840553/140‑1 (MQ=255)

         gTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATgg                                                                                                                                   >  1:1509327/1‑140 (MQ=255)

          ttAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCGATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGa                                                                                                                                  >  2:31987/1‑140 (MQ=255)

             aCTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCa                                                                                                                               <  1:1875408/140‑1 (MQ=255)

             aCTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCa                                                                                                                               <  1:3737924/140‑1 (MQ=255)

             aCTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCa                                                                                                                               <  1:2871528/140‑1 (MQ=255)

             aCTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCa                                                                                                                               <  1:2620082/140‑1 (MQ=255)

               ttCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATg                                                                                                                             <  2:2059714/140‑1 (MQ=255)

                 cGATTGGTGTTCATCGGTTTATGTCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAg                                                                                                                           <  2:1419362/140‑1 (MQ=255)

                 cGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAg                                                                                                                           <  2:3660667/140‑1 (MQ=255)

                 cGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAg                                                                                                                           <  2:3391240/140‑1 (MQ=255)

                 cGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAg                                                                                                                           <  2:328016/140‑1 (MQ=255)

                 cGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAg                                                                                                                           <  2:2602707/140‑1 (MQ=255)

                 cGATTGGTGTTCATCGGTTTATGCCGTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATATATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAg                                                                                                                           <  1:2277102/140‑1 (MQ=255)

                 cGATTGGTCTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAg                                                                                                                           <  2:3533998/140‑1 (MQ=255)

                       gtgtTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTa                                                                                                                     <  2:2729386/140‑1 (MQ=255)

                       gtgtTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTa                                                                                                                     <  1:4208271/140‑1 (MQ=255)

                             aTCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATATATAAAGCCATTAACTTTAAGCAATTTTTTAATTCACATATGGAGCATGATCTTTTACAGTCt                                                                                                               >  2:2341491/1‑140 (MQ=255)

                             aTCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAACAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAAATTTTTAATGCAGATCTGGAGCATGATCTTTTAAAGTCt                                                                                                               >  2:4595821/1‑140 (MQ=255)

                                        ccTTCCGCTGTAGATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATgg                                                                                                    <  2:5028602/140‑1 (MQ=255)

                                           tCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGgcag                                                                                                 <  1:1680285/140‑1 (MQ=255)

                                            ccGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGTTGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATTCAGATATTGAGCATGAGCTTTTAAAGTCTTACCAAAATTgcagc                                                                                                >  1:3956959/1‑140 (MQ=255)

                                            ccGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATg                                                                                                                                            >  2:1760303/1‑96 (MQ=255)

                                            ccGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAAACTATAAATCCATTAACTTTAAGCAATTTTTTAATGAAGATATTGAGCATGAGCTTTTACAGTCTTAGCAAAATTgcagc                                                                                                >  1:4859382/1‑140 (MQ=255)

                                            actcTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTTATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGgcagc                                                                                                <  1:1816249/137‑1 (MQ=255)

                                                       aaTCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGaa                                                                                     >  2:3491377/1‑140 (MQ=255)

                                                       aaTCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGaa                                                                                     >  2:487491/1‑140 (MQ=255)

                                                       aaTCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAAACTATAAAGCCATTAACGTTAAGCAATTTTTTAATTCAGATATGGAGCAAGAGCTTTTACAGTCTTACCAAAATTGCAGCAAATCTGTGaa                                                                                     >  1:1213375/1‑140 (MQ=255)

                                                         tCCTGAGGTGATTCGGCAGTCGTTTTTAAGTGAGGAAGAAATAGAAATATATAAAGCCCTTAACGTTAAGCAATTTTTTTATGCAGATATGGAGCATGAGCTTTTACAGTGTTACCAAAATTGCAGCAAATCTGTTAATc                                                                                   >  1:4754451/1‑140 (MQ=255)

                                                         tCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAATCCATTAACTTTAAGCAAATTTTTAATTCAGATATTGATCATGAGCTTTTACAGTCTTAACAAAAATGCAGCAAAACTGTGAAta                                                                                   >  1:1443258/1‑139 (MQ=255)

                                                         tCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATATATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATc                                                                                   >  1:921143/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAATAAATCTATAAATCCATTAAATTTAAGCAAATTTTTAATGCAGATATTGAGCATGAGCTTTTAAAGAATTACCAAAATGGAAGCAAATATGTTAAACTGc                                                                                >  1:32963/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCCTTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGc                                                                                >  2:2526475/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGc                                                                                >  2:2785936/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGc                                                                                >  2:3045181/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGc                                                                                >  2:738688/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAAAGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTgg                                                                                >  1:3046986/1‑139 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAACCAAATTTTTAATGCAGATATTGAGCATGAGCATTTACATTCTTACCAAAATCGCAGCAAATCTGTTAAACTGc                                                                                >  2:1024578/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAAACTATAAAGCCATTAACTTTAAGCAATTTTTTAATTCAGATATTGAGCATGAGCTTTTAAAGTCTTACCAAAATTGCAGCAAATCTGTGAAACttc                                                                                >  1:1358891/1‑138 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACGGATGAACAAAAAGAAAACTATAAAGCCATTAACTTTAATCAATTTTTTAATGCAGAAATGGAGCATGAGATTTTACAGTCTTACCAAAATGGCAGCAAATATGTGAATCTGc                                                                                >  2:1153776/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTTTTTAACAGATGAAGAAAAAGAAATATATAAAGCCAATAACTTTAAGCAATTTTTTAATGCACAAATGGATCAAGAGATTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGc                                                                                >  2:1351906/1‑140 (MQ=255)

                                                            tGAGGTGATTCGGCAGTCGTGTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGTGCTTTTACAGTCTTACCAAAATGGCAGCAAAGCTCTGAATCttc                                                                                >  1:3694408/1‑138 (MQ=255)

                                                              aGGTGATTCGGCAGTCGTTTTTTACTGATGAAGAAAAAAAAAAAAATAAATCCAATAACTGTAAGCAAATTTTTAATTCAGATATTGATCATGAGCTTTTAAAGTCTTACCACAAAGGCAGCAAAACTTTGAATATGccc                                                                              >  1:576690/1‑139 (MQ=255)

                                                              aGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCCTTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATATGCCg                                                                              >  1:4285085/1‑140 (MQ=255)

                                                              aGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAATCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCg                                                                              >  1:3143925/1‑140 (MQ=255)

                                                              aGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACATTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCg                                                                              >  2:651016/1‑140 (MQ=255)

                                                              aGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAAATTTTTAATGCAGATATGGAGCATGAGCTTTTAAAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCg                                                                              >  1:1624633/1‑140 (MQ=255)

                                                                   aTTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCATATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATATGCCGGCGcc                                                                         >  2:696549/1‑140 (MQ=255)

                                                                   aTTCGGCAGTCGTTTTTAACGGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGcc                                                                         >  2:1885106/1‑140 (MQ=255)

                                                                    ttCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAATCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCa                                                                        >  1:2480996/1‑140 (MQ=255)

                                                                     tCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATATATAAACCCATTAACTTTAAGCAAATTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTAACAAAATGGCAGCAAATATGTGAATCTGCCGGCGCCaa                                                                       >  2:2182429/1‑140 (MQ=255)

                                                                                 tttAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGATCATGAGATTTTACAGTCTTACCAAAATGGCAGCAAAACTGTTAATCTGCCGGCGCCAAAAGAAACTCCCg                                                           >  2:2834251/1‑140 (MQ=255)

                                                                                     aCTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCtt                                                       <  2:3988433/140‑1 (MQ=255)

                                                                                     aCTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCtt                                                       <  2:108648/140‑1 (MQ=255)

                                                                                         atgaAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGAtt                                                   >  1:4429222/1‑140 (MQ=255)

                                                                                          tgaAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGAttt                                                  >  2:3750652/1‑140 (MQ=255)

                                                                                              gaaaaagaaaTCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGa                                              >  1:1740960/1‑140 (MQ=255)

                                                                                                aaaagaaaTATATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATg                                            <  2:4771809/140‑1 (MQ=255)

                                                                                                 aaagaaaTCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAGAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGc                                           >  2:2436380/1‑140 (MQ=255)

                                                                                                 aaagaaaTCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGc                                           >  2:1817116/1‑140 (MQ=255)

                                                                                                      aaTCTATAAAGCCATGAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGtct                                      <  1:1115296/140‑1 (MQ=255)

                                                                                                        tCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTctct                                    <  1:2341491/140‑1 (MQ=255)

                                                                                                         cTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTg                                   >  2:4262625/1‑140 (MQ=255)

                                                                                                           ataAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGAc                                 <  1:3330344/140‑1 (MQ=255)

                                                                                                           ataAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGAc                                 <  2:3915800/140‑1 (MQ=255)

                                                                                                           ataAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGAc                                 <  2:3787546/140‑1 (MQ=255)

                                                                                                           ataAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGAc                                 <  2:3685874/140‑1 (MQ=255)

                                                                                                            taAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGAcc                                >  2:3119700/1‑140 (MQ=255)

                                                                                                                  cATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATa                          <  1:4692842/140‑1 (MQ=255)

                                                                                                                    ttAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGa                        >  2:449320/1‑140 (MQ=255)

                                                                                                                    ttAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGa                        >  1:4148749/1‑140 (MQ=255)

                                                                                                                    ttAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGa                        >  1:713796/1‑140 (MQ=255)

                                                                                                                    ttAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGa                        >  2:1463966/1‑140 (MQ=255)

                                                                                                                    ttAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGa                        >  1:2116139/1‑140 (MQ=255)

                                                                                                                    ttAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTAACAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGa                        >  1:503561/1‑140 (MQ=255)

                                                                                                                        cTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACAtt                    <  1:2425996/140‑1 (MQ=255)

                                                                                                                           tAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCaa                 >  1:3650283/1‑140 (MQ=255)

                                                                                                                           tAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCaa                 >  2:90855/1‑140 (MQ=255)

                                                                                                                           tAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATGTTAGATGCGGTCTCTGACCAAAATAGACATTCaa                 >  2:2929752/1‑140 (MQ=255)

                                                                                                                           tAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGGCTTGATTTTAGATGCGGTCTCTGACCCAAATAGACATTCaa                 >  2:5212029/1‑140 (MQ=255)

                                                                                                                            aaGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCaaa                <  2:159480/140‑1 (MQ=255)

                                                                                                                               cAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTa             <  2:3956959/140‑1 (MQ=255)

                                                                                                                               cAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTa             <  1:487491/140‑1 (MQ=255)

                                                                                                                                 aTTTTTTTATTGAGATATGGATCATGAGATTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGATACTCCCGTCTTGATTTTAGATGCGGTCTCTGAACAAAATAGACATTCAAAGTATg           <  1:2187646/140‑1 (MQ=255)

                                                                                                                                 aTTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTTAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTATg           <  2:1300988/140‑1 (MQ=255)

                                                                                                                                 aTTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTATg           <  1:851236/140‑1 (MQ=255)

                                                                                                                                      ttAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAGATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTATGCCATa      <  2:2256696/140‑1 (MQ=255)

                                                                                                                                         aTGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTATGCCATACaa   <  1:4592804/140‑1 (MQ=255)

GTTCCACCTGTTAACTTCGATTGGTGTTCATCGGTTTATGCCTTCCGCTGTATATAATCCTGAGGTGATTCGGCAGTCGTTTTTAACTGATGAAGAAAAAGAAATCTATAAAGCCATTAACTTTAAGCAATTTTTTAATGCAGATATGGAGCATGAGCTTTTACAGTCTTACCAAAATGGCAGCAAATCTGTGAATCTGCCGGCGCCAAAAGAAACTCCCGTCTTGATTTTAGATGCGGTCTCTGACCAAAATAGACATTCAAAGTATGCCATACAAA  >  NZ_CP034943/209673‑209950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`