BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,211	G→T	11.6%	A107A (GCC→GCA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,211	0	G	T	11.6%	788.4 / 28.8	365	A107A (GCC→GCA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (105/205); new base T (52/2); total (158/207)
Fisher's exact test for biased strand distribution p-value = 2.13e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

AGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGA  >  NZ_CP034943/240077‑240347

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATTCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTGAAGTTTTGGTTGATTTTACTGGGGTTTc                                                                                                                                   >  1:2489404/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTc                                                                                                                                   >  2:65100/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCGTTTc                                                                                                                                   >  2:4543786/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTc                                                                                                                                   >  2:540202/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGTCTTTTc                                                                                                                                   >  2:2023219/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTc                                                                                                                                   >  2:223479/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                   >  2:3965759/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTGGGTGATTTGAATGGCttttg                                                                                                                                   >  1:3236898/1‑139 (MQ=255)

  agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTc                                                                                                                                   >  2:4723683/1‑140 (MQ=255)

  agagaAGGAGCATCTCTGATGGTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTTTTTGTAGTTTTGGGGGATTTGAATGGGTTTTc                                                                                                                                   >  1:2191008/1‑140 (MQ=255)

    agaAGGAGCATCTATGATGTTGTAATTATTCAGAGAGGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCAATCCGACTATACGGGCGTGTTAATATTTTTTTTTGAATTTTTTGGATATTTTAATAGCTATTACg                                                                                                                                 >  1:3034360/1‑140 (MQ=255)

        ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGtttt                                                                                                                             >  1:1090747/1‑140 (MQ=255)

         gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGttttt                                                                                                                            >  2:3621735/1‑140 (MQ=255)

          aGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCTTTTTCGGATTTTTGTTTTAAGTTTTTGGAGAATTGAATTGCTTTTCAGTTTTTa                                                                                                                           >  2:3216773/1‑140 (MQ=255)

             aTCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTaaaa                                                                                                                        >  1:4811646/1‑140 (MQ=255)

              tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTGGGAGATTTTAATGGCTTTTCCTTTTTTaaaaa                                                                                                                       >  2:1349484/1‑139 (MQ=255)

               ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGTGAGATTTTAATTGCTTTTCCGTTTTTAAAACa                                                                                                                      >  2:2105715/1‑140 (MQ=255)

                tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACaa                                                                                                                     >  2:4076937/1‑140 (MQ=255)

                tctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAaaaaaa                                                                                                                     >  2:2060234/1‑140 (MQ=255)

                    aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAagacg                                                                                                                 <  1:253267/140‑3 (MQ=255)

                    aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                                 <  1:3042833/140‑1 (MQ=255)

                    aTGTTGGAATTAGTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                                 <  1:3145365/140‑1 (MQ=255)

                        tGGAATTCTTCAGAGATGCTGTTTGTCTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTACGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  1:4099212/140‑1 (MQ=255)

                        tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATGCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  2:2312002/140‑1 (MQ=255)

                        tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  2:1210779/140‑1 (MQ=255)

                        tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                             <  1:1138804/140‑1 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCTATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAATTTTTTGTAGATTTTAATTGATTTTCCCTTTTTAAAACAAGATGCaaac                                                                                                            >  1:4839774/1‑138 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTGTGATATTTTTATTGCTTTTGCGTTTTTAAAAAAAAAGGCaaca                                                                                                            >  1:2573611/1‑138 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATTGGTTTTGCGTTTTTAAAAAAAGAGGCaaac                                                                                                            >  1:2703137/1‑138 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGATATTTGAATGGCTTTTACGTTTTTAAAAAAAGAGGCAAGc                                                                                                            >  2:2393179/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTTGAGATTTGAATTGCTTTTGCGTTTTTTAAACAAGAGGCAAGc                                                                                                            >  1:2822378/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                            >  1:1252878/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAAAAAGTGGCAAGc                                                                                                            >  2:1554387/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                            >  2:1815527/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGGTTTGTGAGATTTGAATGGCGTTTCCGTTTTTAAAACAAGAGGCACGc                                                                                                            >  2:1018151/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTAAAAAAAGATGCAAGc                                                                                                            >  1:3368150/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTAGGATTTTTGTTTGTAGTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTAAAACAAGAGGGAAGc                                                                                                            >  1:65799/1‑140 (MQ=255)

                         ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                            >  2:2665874/1‑140 (MQ=255)

                                     agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCa                                                                                                               >  1:531887/1‑125 (MQ=255)

                                        tGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAAACCcaca                                                                                             >  1:3848855/1‑140 (MQ=255)

                                                       tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAATAGGCAAGCCAGATAAACCCGACAAAAAAAAAAaaaaat                                                                              >  2:2720317/1‑140 (MQ=255)

                                                       tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTTAAACAAGATGCAAGCCAGATAAAAACGACAACAACAAAAaataat                                                                              >  2:1119275/1‑140 (MQ=255)

                                                       tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                              >  2:2697081/1‑140 (MQ=255)

                                                       tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                              <  2:684097/140‑1 (MQ=255)

                                                       tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                              >  2:5102484/1‑140 (MQ=255)

                                                           ttCTAATCCGAAGTCGATCATTCCGACTATCCGGGCGTTTTCGGCTTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:4906465/140‑1 (MQ=255)

                                                           ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  2:4489282/140‑1 (MQ=255)

                                                           ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:2218031/140‑1 (MQ=255)

                                                           ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGATTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                          <  1:4368465/140‑1 (MQ=255)

                                                                 tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                    <  2:2433595/140‑1 (MQ=255)

                                                                 tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                    <  2:440683/140‑1 (MQ=255)

                                                                 tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTGGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                    <  1:193813/140‑1 (MQ=255)

                                                                   cGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaaa                                                                  <  1:5206495/140‑1 (MQ=255)

                                                                   agAAGTCGATCTTTGAGACTATACGGGCTTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaaa                                                                  <  1:3300229/139‑1 (MQ=255)

                                                                       gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                              <  1:861959/140‑1 (MQ=255)

                                                                           aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                                                          >  2:2821074/1‑140 (MQ=255)

                                                                            tCATTCCGACTATTATGGAGTTTTAGTATTTTTGTTTGAAGTTGTGGGAGATTTGAATGGCTTTTCTGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATATAGCAAGTAAACTCcatgca                                                         <  1:4897386/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:3521034/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:4543786/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:2098831/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:4151505/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:3355495/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:3163470/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:2501686/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:3494919/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:3604299/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:3671155/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  1:380335/140‑1 (MQ=255)

                                                                            tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATGTGAATGGCTTTTCCGTTTATAAAACAAGAGGCAAGCCATATAAACCCGCCAACAACAAAAAATAATCTAGCAAGTATACTCcatgca                                                         <  1:5067970/140‑1 (MQ=255)

                                                                            tCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                         <  2:5165732/140‑1 (MQ=255)

                                                                                tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                                                     <  2:1414678/140‑1 (MQ=255)

                                                                                         aCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTTATTGCTTTTACGTTTTTTAAAAAATAGGCAACCCCCATAAACCCCACAACAAAAAAAAAAAATATAGCAAGTAAACACCATGCATGGGGtaattaat                                            >  1:4621661/1‑140 (MQ=255)

                                                                                          cGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCAAGCATGGGGTAattcatt                                           >  2:3275187/1‑140 (MQ=255)

                                                                                                  ttCTGATTTTTGTTTGAAGTTTTGTGAGATTTGAATGTCTTTTCCGTTTTTAAAACAAGAGGCAATCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                   <  1:2892009/140‑1 (MQ=255)

                                                                                                  ttCGGATTTTTGTTTGAAGTTTTGGGAGTTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCCGATAAACCCGACAACAACAAAAAATAATCTAGCCAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                   >  2:3814506/1‑140 (MQ=255)

                                                                                                  ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                   <  1:2607544/140‑1 (MQ=255)

                                                                                                      gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                               <  2:1594944/140‑1 (MQ=255)

                                                                                                      gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                               <  1:1538117/140‑1 (MQ=255)

                                                                                                      gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                               <  1:1581207/140‑1 (MQ=255)

                                                                                                      gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                               <  1:469175/140‑1 (MQ=255)

                                                                                                      gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                               <  2:5198013/140‑1 (MQ=255)

                                                                                                       aTTTTTGTTTGAAGTTTTGGGAGCTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAatat                              <  1:3959334/140‑1 (MQ=255)

                                                                                                         ttttGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaa                            <  2:4839774/140‑1 (MQ=255)

                                                                                                          tttgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaa                           >  2:4995996/1‑140 (MQ=255)

                                                                                                             gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCAGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                        <  1:131967/140‑1 (MQ=255)

                                                                                                              tttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATa                       <  1:1355228/140‑1 (MQ=255)

                                                                                                               ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                      <  2:4899420/140‑1 (MQ=255)

                                                                                                               ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                      <  2:78346/140‑1 (MQ=255)

                                                                                                                  aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                   <  1:2393179/140‑1 (MQ=255)

                                                                                                                  aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                   <  1:4187846/140‑1 (MQ=255)

                                                                                                                          ggAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAtg           <  1:1646156/140‑1 (MQ=255)

                                                                                                                          ggAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAtg           <  2:2954926/140‑1 (MQ=255)

                                                                                                                              aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATTATCTAGCAAGTAAACTCGATGCATGGGGTAATTCAGTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc       <  2:3304542/140‑1 (MQ=255)

                                                                                                                              aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc       <  1:1815527/140‑1 (MQ=255)

                                                                                                                              aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGcc       <  1:1844816/140‑1 (MQ=255)

                                                                                                                               tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg      <  2:1291094/140‑1 (MQ=255)

                                                                                                                               tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGACAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg      <  2:4748483/140‑1 (MQ=255)

                                                                                                                               tttGAATGGCTTTTCCGTTATTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCAGGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg      >  2:1765788/1‑140 (MQ=255)

                                                                                                                                 tGAATGGCTTTACCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTa    <  2:4624171/140‑1 (MQ=255)

                                                                                                                                   aaTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGa  >  2:4244479/1‑140 (MQ=255)

AGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTAGA  >  NZ_CP034943/240077‑240347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`