Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,270,0640GC29.5% 296.3 / 39.9 186L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base G (65/57);  new base C (0/51);  total (66/120)
Fisher's exact test for biased strand distribution p-value = 1.55e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 206 total aligned reads displayed.

ACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATATT  >  NZ_CP034943/2269925‑2270202
                                                                                                                                           |                                                                                                                                          
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAACAAGAAACGAAAAAGATTCCGCCTCCCACAATCTCTTGTTCAAGCACGTACTGGATAAACATTGTGGGACATACAAGAACAACCCCTAg                                                                                                                                            >  1:3436200/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCCCGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                            >  1:1211677/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                            >  2:2867188/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                            >  1:868652/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                            >  1:299069/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                            >  2:2996353/1‑140 (MQ=255)
aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                            >  1:2324193/1‑140 (MQ=255)
aCCATCAGAATAAACCATGTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGCAAAAGCTTCCGCCTCGCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTCTGGGACATAGAAGAACAACCCGTAg                                                                                                                                            >  2:2910948/1‑140 (MQ=255)
     cAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGaaaaa                                                                                                                                       >  2:3724285/1‑140 (MQ=255)
       gAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAAc                                                                                                                                     >  1:3683594/1‑140 (MQ=255)
          tAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAc                                                                                                                                  >  1:2062321/1‑140 (MQ=255)
            aaCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCg                                                                                                                                >  2:650351/1‑140 (MQ=255)
             aCCATTTTAATTATTCCTCCAAAACCCCTGAAGATAAAAAACAAACGAAAACGCTTCCGCCTCCCACATTCTATTTTTCAAGCACGTACTGGATAAACATTGTGGGAAAAACAAGAACACCCCCTACAAAAAACGACCGa                                                                                                                               <  1:3755463/140‑1 (MQ=255)
               cATTTTAATGATTCCTCCAAAAACCCTGAATATAAAAAACAAACGAAAACGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAg                                                                                                                             <  2:868652/140‑1 (MQ=255)
                tttttATTTATTCCACAAAAAACCCTTAACATAAAAAACAAACTAAACCGGTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAAATTTGTGGTAAATACAACAACAACCCCTAAAAAAAACGACCGAAGc                                                                                                                            <  1:753615/139‑1 (MQ=255)
                tttttAATGATTCCTCCAAAACCCCTCAACATAAAAAACAAACCAAAACCCTCCCGCCCCCAACATTCTCTTTTTAAACCACGTACTGTATAAACATTTGGGCAAATACAACAACACCCCCCAAAAAAAACGACCGAAGc                                                                                                                            <  1:989475/139‑1 (MQ=255)
                tttttAATGATCCCTCCAAAACCCCTCAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGAAGc                                                                                                                            <  1:765938/139‑1 (MQ=255)
                aTTTTAATTTTTCCCCAAAAACCCCTCAATAAAAAAAACAAACTAAAAAGCTACCGCCCCCCACATTATCTTTTTCATCCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTACAAAACACGACCGAAGc                                                                                                                            <  1:2435188/140‑1 (MQ=255)
                aTTTTAATTATTCCTACAAAAACCATGAATATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCAATTTCTCTTTTTCAAGCACGTACTGTATAAACTTTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                            <  2:2393003/140‑1 (MQ=255)
                aTTTTAATGATTCCTCAAAAAACCCTGAAGATAAAAAATAACCGAAAACGCTTCCGCCTCCATCATTGTATTTTTCAAGCACTTACTGGATAAAATTTGTGGTAATTACAACAACACCCCCTAGAAAAAACGACCGAAGc                                                                                                                            <  2:591474/140‑1 (MQ=255)
                  tttAATTATTCATCAAAAAACCCTGAAGATAAAAAATAAACGAAAATGGTTCCGCCTCCCACATTCTCTTTTTCAAGCACTTAGTGGATAAACTTTGTGGTAAATACAATAACAACCCCTATAAAAAACGACCGAAGCAc                                                                                                                          <  2:191724/140‑1 (MQ=255)
                  tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                                          >  1:19014/1‑140 (MQ=255)
                   ttatttaTTCATCAAAAAACCTTTAATAAAAAAAACAAACCAAAAAGCTTCCGCCTCACAATTTCTGTTTTTCAAGCACGTACTGTATAAAATTTGTGGTAAATACAACAACAACCCATCCAAAAAACGACCGAAGCAcc                                                                                                                         <  1:3042678/134‑1 (MQ=255)
                   ttAATTATTCCACCAAAAACCCTCAATATAAAAAAAAAACCAAAACGCGCCCGCCTCCCACATTCTATTTTTCAAGCACGTACTGGATAAACATTGTGTTAAAAACAACAACAACCCCTACAAAAAACGACCGAAGCAcc                                                                                                                         <  1:820758/140‑1 (MQ=255)
                   ttAATTATTCATCAAAAAACCCTCAAAAAAAAAATAAACCTAAAACGCTGCCGCATCCCACTTTCTCTTTTTCAAGCACCTACTGGTAAAAACTTGTGGTAATTACAACAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                         <  1:1883789/140‑1 (MQ=255)
                   ttAATGATTCCTCCAAAAACCCTTAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATTCTGTTTTTAAAGCACGTACTGGATAAACATTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                         <  1:3865127/140‑1 (MQ=255)
                   ttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                         <  2:2734325/140‑1 (MQ=255)
                   ttAATGATTCATCAAAAAACCCTGAACATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTATCTTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCAcc                                                                                                                         <  2:2453624/140‑1 (MQ=255)
                     atttaTTCCTCCAAAAACCCTTAATATAAAAAATAAACTAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACTTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTATAAAAAACGACCGAAGCACCat                                                                                                                       <  2:1484005/136‑1 (MQ=255)
                     aaTTATTCCTCCAAAACCCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACGGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                                       <  2:1632061/140‑1 (MQ=255)
                     aaTTATTCCTCCAAAACCCCTGAAGAAAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                                       <  2:770343/140‑1 (MQ=255)
                     aaTGATTCCTCAAAAAACCATGAAGATAAAAAAGAAACCAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTAAAGAACGTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                                       <  2:2321486/140‑1 (MQ=255)
                     aaTGATTCATCCAAAAACCCTCAAGATAAAAAAGAAACGAAAACGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAAGACCAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                                       <  2:2219466/140‑1 (MQ=255)
                          ttccaaaaaaaaCCCTCAATAAAAAAAAAAAACCAAAAAGCTTCCGCCTCCCAAATTGTCTTTTTAAAGCACGTACTGTATAAACATTGTGGTAAATACACCAACACCCCCTCCAAAAAACGACCGAAGCACCATATGTa                                                                                                                  <  1:355093/133‑1 (MQ=255)
                          tccctcaAAAACCCCTCAATAAAAAAATCAAACCAAAAAGGTTCCGCCTCCCAAATTTTATTTTTCAAGCACGTACTGTATAAACTTTGTGGTAAATACAAGAAAACCCCCTACAAAAAACGACCGAAGCACCATATGTa                                                                                                                  <  1:1960527/138‑1 (MQ=255)
                          tacacccAAAACCCCTCAAGATAAAAAACAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGCATAAACATTGTGGCAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTa                                                                                                                  <  1:2677170/135‑1 (MQ=255)
                           tcctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                                 >  1:60160/1‑140 (MQ=255)
                           tcctcaAAAAACCCTCAACATAAAAAAAAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCCCGTACTGGATAAACATTGTGGGAAATACACCAACACCCCCTACAAAAAACGACCGAAGCACCATATGTAc                                                                                                                 <  1:2633284/140‑1 (MQ=255)
                            cctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACa                                                                                                                >  1:2165836/1‑140 (MQ=255)
                            cctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACa                                                                                                                >  1:1940668/1‑140 (MQ=255)
                              tccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAgg                                                                                                              >  1:2383270/1‑140 (MQ=255)
                                cAAAACCCCTCACCATAAAAACCAACCCAAAAACCTGCCCCCCCCCACATTCTCTTTTACAAGCACGTACTGGATAAACATTGTGGGAAATAAAACACCAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                            <  1:2858426/140‑1 (MQ=255)
                                cAAAACCCCTCAAGATAAAAAATAAACCACAACCCCGCCCCCACCCAACTACTCTTTTTCAAGCACGTACTGCATAAACATTGGGGGAAATCCACCAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                            <  1:3313426/140‑1 (MQ=255)
                                cAAAAACCCTTATTATAAAAAAGAAACTAAAAAGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTATAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                            <  2:60160/140‑1 (MQ=255)
                                cAAAAACCCTGAATATAAAAAAGAAACTAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                            <  2:3078261/140‑1 (MQ=255)
                                aaaaacCCCTTAATAAAAAAAATAAACCAAAAAGCTTCCGCCTCCCAAATTATATTTTTAAAGCACGTACTGGATAAACTTTGTGGCAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                            <  1:1690145/139‑1 (MQ=255)
                                aaaaaaCCCTGAATATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                            <  2:1110422/139‑1 (MQ=255)
                                aaaaaaCCCTCAAGATAAAAAATAAACCAAAAAGCTTCCGCCTCCCACTTTATCTTTTTCAAGCACTTACTGGATAAACATTTTGGTAAATACAACAAAAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGtt                                                                                                            <  2:435959/139‑1 (MQ=255)
                                  aaaaCCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAcctgtctc                                                                                                          >  1:1614714/1‑133 (MQ=255)
                                     aCCCTGAATATAAAAAAGAAACCAAAAACCTACCGCCTCCCACATTATCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                                       <  2:3683594/140‑1 (MQ=255)
                                        cTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGAGCGAAGCACCATATGTACAGGTTCATTATCa                                                                                                    >  2:1996981/1‑140 (MQ=255)
                                        cTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCa                                                                                                    >  2:610887/1‑140 (MQ=255)
                                                           gAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                 <  2:2086711/140‑1 (MQ=255)
                                                           caacaaGCTCCCGCCACCCACATTCTCTTTTTCAAGCACGTACTGGATAAAATTTGTGGGAAATACAACAACACCCCATATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                 <  1:1866767/136‑1 (MQ=255)
                                                           caaaacGCTTCCGCCTCCCACATTATCTTTTTCAAGCACGTACTGTATAAACTTTGTGGTAAATACAATAACACCCCCTATAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                                 <  1:2910948/139‑1 (MQ=255)
                                                                   ttCCGCCTCCCCACTTATCTTTTTCAACCCGTTACTGGATAAACATTGTGGGAAATACAATAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACtt                                                                         <  2:3211677/140‑1 (MQ=255)
                                                                   gtCCGCCTCCCCAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACACGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACtt                                                                         <  2:935632/139‑1 (MQ=255)
                                                                     ccGCCTCCAACATTCTCTTTTTAAAGAACTTAGTGGATAAACATTGTGGTAAATAAAATAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTaa                                                                       <  1:435504/140‑1 (MQ=255)
                                                                                      tttttCACGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAg                                                      >  2:3004492/1‑140 (MQ=255)
                                                                                      tttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAg                                                      >  1:2997012/1‑140 (MQ=255)
                                                                                       ttttCAAGCACGTACTGTATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                                     <  2:3561297/140‑1 (MQ=255)
                                                                                       ttttCAAGCACGTACTGCATAAACATTGTGGCAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                                     <  1:562569/140‑1 (MQ=255)
                                                                                       ttttAAAGCACTTACGGTATAAACATTGTGGGAAACCCAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGAGGCCGGGAGc                                                     <  1:213629/140‑1 (MQ=255)
                                                                                          tCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                                  <  2:2504603/140‑1 (MQ=255)
                                                                                          tCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCTTACTGATCTTTTACAGAGAAATAACTTAAGAGCGCGATGCGGGGTGCCAt                                                  >  2:3418145/1‑140 (MQ=255)
                                                                                          acaaccccGTACTGGATAACCTTTGTGGGAAATACAAGAACAACCCCTCCAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                                  <  1:1786319/133‑1 (MQ=255)
                                                                                              gCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCt                                              >  1:1310267/1‑140 (MQ=255)
                                                                                                  gTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCag                                          >  2:137118/1‑140 (MQ=255)
                                                                                                    aCTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCagag                                        >  1:3840733/1‑140 (MQ=255)
                                                                                                        gATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCCCCATATGTACAGGTTCATAATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgatga                                    >  1:1988307/1‑140 (MQ=255)
                                                                                                        gATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTGTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgatga                                    >  2:1944604/1‑140 (MQ=255)
                                                                                                          tAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAtt                                  >  2:3529343/1‑140 (MQ=255)
                                                                                                             aCATTTTGTTAAATACAAGAACAACCCCTAGAAAAAACGACCGAATCACCATATGTACATGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                               <  1:3642047/140‑1 (MQ=255)
                                                                                                             aCATTGTGGTAAATACAACAACAACCCCTACAACAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGAGGCCGGGAGCAATTCCTGCAGAGATGATTGCa                               <  1:1237248/140‑1 (MQ=255)
                                                                                                             aCATGGGGGTAAATACAAGACCAACCCCGACAAAAAACGACCGACGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                               <  1:2502963/140‑1 (MQ=255)
                                                                                                                  gtgttaaaTACAATAAAGACCCCTATAAAAAACTACAGAAGCACAATATGTACAGGTTCATTAGAAGACTGAACTTTTAAATAAAAATAAATAAACGGCTCTATGACCTGAACAATTAATGCAGAGAAGTTAGAAAACAt                          >  1:3645276/6‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAATAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          <  1:3529343/140‑1 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACCACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCAGACTGATCTTTTCAATACAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGAGTGCAAACAt                          >  2:3259825/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACCGGTGCATTATCATACTGATCTTTTAAATACAAATAACTTAACAGCGCGATGCCGGGAGCAATGCCTGCAGAGATGATTGCAAAGAt                          >  1:996052/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          >  1:1008878/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          >  2:2766539/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          >  1:3193331/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          >  1:1501836/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCAGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          >  1:1581624/1‑140 (MQ=255)
                                                                                                                  gtgGGAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          <  1:2607411/140‑1 (MQ=255)
                                                                                                                  gtgGGAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt                          <  1:1074281/140‑1 (MQ=255)
                                                                                                                       aaaCACAACCACACCCCCGACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGc                     <  1:3003966/140‑1 (MQ=255)
                                                                                                                        aaTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCa                    >  1:2279496/1‑140 (MQ=255)
                                                                                                                              acaacaacCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAATATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct              <  2:548998/138‑1 (MQ=255)
                                                                                                                              aGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct              <  2:1008878/140‑1 (MQ=255)
                                                                                                                              aGAACAACCCCTACAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct              <  1:3500154/140‑1 (MQ=255)
                                                                                                                              aGAACAACCCATACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct              <  1:2264951/140‑1 (MQ=255)
                                                                                                                                  caacCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTcc          >  2:3817955/1‑140 (MQ=255)
                                                                                                                                    acCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACTGCGCGATGCCGGGAGCACTTCCTGCAGAGATGATTGCAAACATCAGGCACCCTCTTTCCtt        >  2:174325/1‑140 (MQ=255)
                                                                                                                                    acCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCtt        >  2:1341874/1‑140 (MQ=255)
                                                                                                                                     ccccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCttt       >  2:746915/1‑140 (MQ=255)
                                                                                                                                       ccTAGAAAAAACGACCGACGCACCATATGGACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtat     <  2:1096895/140‑1 (MQ=255)
                                                                                                                                       ccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtat     <  2:346777/140‑1 (MQ=255)
                                                                                                                                       ccTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtat     <  2:1846434/140‑1 (MQ=255)
                                                                                                                                        cTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTtata    <  1:324619/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCTTTCCTTTATATT  >  NZ_CP034943/2269925‑2270202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑