Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 44,881 G→A intergenic (‑62/‑439) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494344,8810GA99.0% 711.3 / ‑2.8 190intergenic (‑62/‑439)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (94/94);  total (95/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 190 total aligned reads displayed.

AGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTTATAAATT  >  NZ_CP034943/44745‑45016
                                                                                                                                        |                                                                                                                                       
agTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                                      <  1:55377/140‑1 (MQ=255)
  tACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACaaa                                                                                                                                    >  1:984850/1‑140 (MQ=255)
   acgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGTTTTCATAAACTTTCTCCTCCCAAGAGAGGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACaaaa                                                                                                                                   >  1:1790915/1‑140 (MQ=255)
   acgacgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACaaaa                                                                                                                                   >  2:2111590/1‑140 (MQ=255)
       cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCGTTATTCAACAAAATCCt                                                                                                                               <  1:430825/140‑1 (MQ=255)
       cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                               <  2:3701100/140‑1 (MQ=255)
        gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                                              <  1:1202013/140‑1 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                            >  1:1378959/1‑140 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                            >  1:1070228/1‑140 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                            >  1:2626762/1‑140 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                            >  2:3140336/1‑140 (MQ=255)
            ttCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCt                                                                                                                          >  2:126305/1‑140 (MQ=255)
               gATCGGAATAACTACAAGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCa                                                                                                                       <  2:2519802/140‑1 (MQ=255)
               gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCa                                                                                                                       <  1:227544/140‑1 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                                     <  2:1359799/140‑1 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                                     <  1:270909/140‑1 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                                     <  1:126305/140‑1 (MQ=255)
                    gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCa                                                                                                                  >  1:2980726/1‑140 (MQ=255)
                         aCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACa                                                                                                             >  1:3122594/1‑140 (MQ=255)
                          cTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATc                                                                                                                                 >  2:748972/1‑119 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                               >  2:288178/1‑117 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                        <  2:327811/140‑1 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                        >  2:61423/1‑140 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                        >  2:3187460/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                                      <  1:1930426/109‑1 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                                      >  2:1930426/1‑109 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                       >  1:1176492/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                       >  2:1874071/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                       >  2:592874/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                       >  2:2833614/1‑140 (MQ=255)
                                  ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGTTGATTATTTGTTTAAATTATATTTTTCGTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                                    >  1:1722346/1‑140 (MQ=255)
                                   cTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaa                                                                                                   >  2:56141/1‑140 (MQ=255)
                                     aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCtt                                                                                                                         >  2:3403376/1‑116 (MQ=255)
                                     aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaata                                                                                                 >  2:3814616/1‑140 (MQ=255)
                                     aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaata                                                                                                 >  1:827271/1‑140 (MQ=255)
                                     aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaata                                                                                                 >  2:1576988/1‑140 (MQ=255)
                                     aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaata                                                                                                 >  1:2475106/1‑140 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                               <  1:2871830/105‑1 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                            >  1:3587157/1‑140 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                            >  2:3529439/1‑140 (MQ=255)
                                           tcaACTTTACGTAAAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTATTCGTAATTATTCAACAAAATCCTTACCTTAATATCATAACAAGGTTTGCaataatattc                                                                                           >  2:2133618/1‑136 (MQ=255)
                                            caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                        >  1:1218534/1‑126 (MQ=255)
                                            caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                        <  2:1218534/126‑1 (MQ=255)
                                            caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                                          >  1:1335410/1‑140 (MQ=255)
                                            caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTATTAGTCATTATTCAACAAAATCCTTACCTTCATAGACTAACAATGTTTGCAATACTCGTCa                                                                                          >  1:3446734/1‑140 (MQ=255)
                                                  tACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtt                                                                                    >  1:3724053/1‑140 (MQ=255)
                                                    cttACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtttt                                                                                  <  2:2565108/138‑1 (MQ=255)
                                                    cGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtttt                                                                                  >  2:2342024/1‑140 (MQ=255)
                                                      tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                                <  1:704718/140‑1 (MQ=255)
                                                      tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                                <  2:2505567/140‑1 (MQ=255)
                                                      tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                                <  2:2749484/140‑1 (MQ=255)
                                                      tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                                <  2:515549/140‑1 (MQ=255)
                                                       aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                                               <  2:2388315/140‑1 (MQ=255)
                                                       aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                                               <  1:2164458/140‑1 (MQ=255)
                                                         aaTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtttg                                                                             >  1:3257196/1‑140 (MQ=255)
                                                          aTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGc                                                                            >  2:144209/1‑140 (MQ=255)
                                                                   gATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                                <  1:1575193/127‑1 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                                 <  1:56141/140‑1 (MQ=255)
                                                                      ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAATGTTTCCAATAATCGTCAATCATTTTGTTTTCTATTGATTGTaa                                                                >  1:992502/1‑140 (MQ=255)
                                                                      ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                                                >  2:3347934/1‑140 (MQ=255)
                                                                        cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGa                                                              >  1:3621330/1‑140 (MQ=255)
                                                                        cATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGa                                                              >  1:2439306/1‑140 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                           <  2:3725844/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                           <  1:800273/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                           <  1:3814616/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                           <  2:827271/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATGTTGTTTGCTATTGATTGTAAGAAtt                                                           >  2:1603960/1‑140 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATACTCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                         <  1:61423/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                         <  2:771593/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                         <  2:2439306/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                         <  2:3723737/140‑1 (MQ=255)
                                                                                 tctcCTCCCAAGAGAGGAGTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCt                                                     >  1:1427137/1‑140 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCGTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                                    <  1:608126/140‑1 (MQ=255)
                                                                                   tcctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                                   <  2:3593255/140‑1 (MQ=255)
                                                                                   tcctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                                   <  2:984850/140‑1 (MQ=255)
                                                                                    cctccCAACAGATGATTATTTGATTAAACTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                                  <  2:3458449/140‑1 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                               >  1:2610901/1‑140 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                               <  1:3187460/140‑1 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                               >  1:1999657/1‑140 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                               <  1:453501/140‑1 (MQ=255)
                                                                                       cccAACAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCGTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAGTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                               <  1:2918015/140‑1 (MQ=255)
                                                                                          aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                                            <  2:1790915/140‑1 (MQ=255)
                                                                                          aaGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAg                                            <  2:1139/140‑1 (MQ=255)
                                                                                           ccagagtattatgTGTTTCTATTATCTTGTTCTTCGTCATTATTCAACAACATCCTTACCGTCATAGCATAACAAGGTTTCCAATAATCCTCAAGCATTTTGGTTGCTATTGATAGGAAGAAGTAGTTCTTATAGTCAGt                                           <  2:1108890/133‑1 (MQ=255)
                                                                                              gatgatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATCATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                                        <  1:3349059/140‑1 (MQ=255)
                                                                                                 gatTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTa                                     >  2:3201868/1‑140 (MQ=255)
                                                                                                  attattTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTATTTCTTATAGTCAGTAATGTAt                                    >  2:2268436/1‑140 (MQ=255)
                                                                                                  attattTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTAt                                    >  2:239826/1‑140 (MQ=255)
                                                                                                      tttGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGa                                <  1:2111590/140‑1 (MQ=255)
                                                                                                         gTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAac                             >  1:2820626/1‑140 (MQ=255)
                                                                                                            tAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactac                          <  2:208241/140‑1 (MQ=255)
                                                                                                            tAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactac                          <  1:2561744/140‑1 (MQ=255)
                                                                                                             aaaTTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactact                         <  2:2435540/140‑1 (MQ=255)
                                                                                                             aaaTTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactact                         <  2:1208382/140‑1 (MQ=255)
                                                                                                               aTTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGa                       <  1:3723388/140‑1 (MQ=255)
                                                                                                                    atTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATaa                  <  2:1486269/140‑1 (MQ=255)
                                                                                                                        ttcttcGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGc              >  2:3677647/1‑140 (MQ=255)
                                                                                                                             cGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCttttt         >  1:692486/1‑140 (MQ=255)
                                                                                                                               tCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTtat       <  1:3347934/140‑1 (MQ=255)
                                                                                                                                cATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTtata      <  1:1874071/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
AGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTTATAAATT  >  NZ_CP034943/44745‑45016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: