Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 131,432 T→A S309T (TCT→ACT)  fusA → elongation factor G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943131,4320TA99.4% 1212.2 / NA 321S309T (TCT→ACT) fusAelongation factor G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (155/164);  minor base C (1/1);  total (156/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

CAAAGGTGTTCAACTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCTCTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAGCGCGTTGGACGTAT  >  NZ_CP034943/131299‑131568
                                                                                                                                     |                                                                                                                                        
cAAAGGTGTTCAACTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCAt                                                                                                                                    >  1:295253/1‑140 (MQ=255)
        ttCAACTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCAttt                                                                                                                            >  1:1072852/1‑140 (MQ=255)
        ttCAACTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCAttt                                                                                                                            <  2:2610030/140‑1 (MQ=255)
        ttCAACTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCAttt                                                                                                                            >  2:2458412/1‑140 (MQ=255)
         tCAACTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTa                                                                                                                           <  2:469083/140‑1 (MQ=255)
             cTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGt                                                                                                                       <  2:674958/140‑1 (MQ=255)
             cTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGt                                                                                                                       <  2:174073/140‑1 (MQ=255)
             cTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGt                                                                                                                       <  1:3868192/140‑1 (MQ=255)
             cTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGt                                                                                                                       <  1:1762290/140‑1 (MQ=255)
             cTAGTACTGGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGt                                                                                                                       <  2:2237776/140‑1 (MQ=255)
              tAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGtt                                                                                                                      <  1:1612054/140‑1 (MQ=255)
                gTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGt                                                                                                                       <  2:1989671/137‑1 (MQ=255)
                gTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAt                                                                                                                    >  2:204241/1‑140 (MQ=255)
                gTACTTGAGGCTGTTCTTGACTACCTTCCTGCACCAACTGACCTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTATGATGAAGAGCCATTCACTGCATAGGCATTTAAAGTTAt                                                                                                                    >  2:1172192/1‑140 (MQ=255)
                   cTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAtgac                                                                                                                 <  1:965138/140‑1 (MQ=255)
                    ttGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCAATGGCATTTAAAGTTAtgact                                                                                                                <  1:1572818/140‑1 (MQ=255)
                      gATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAtgactga                                                                                                              >  1:1679771/1‑140 (MQ=255)
                      gATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAtgactga                                                                                                              >  1:2081536/1‑140 (MQ=255)
                       aTGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAtgactgac                                                                                                             >  2:3402907/1‑140 (MQ=255)
                       aTGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAtgactgac                                                                                                             >  1:249883/1‑140 (MQ=255)
                        tGCTGTTCTTGACGACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACACATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGAcc                                                                                                            <  1:2227062/140‑1 (MQ=255)
                            gTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTa                                                                                                        <  1:602232/140‑1 (MQ=255)
                            gTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTa                                                                                                        <  2:382434/140‑1 (MQ=255)
                             ttCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTAc                                                                                                       >  2:1142368/1‑140 (MQ=255)
                                  gACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCAttt                                                                                                                            <  1:1895076/114‑1 (MQ=255)
                                  gACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCAttt                                                                                                                            >  2:1895076/1‑114 (MQ=255)
                                       ccTTGCTGCACCAAATGACGTTGCTTCAATTAAAGGTACTCGTCCTGATACATATGAAGAGATTTAGCGCGATTCTTCTGCTGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTATGt                                                                                             <  2:3467050/140‑1 (MQ=255)
                                       ccTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             >  1:2988672/1‑140 (MQ=255)
                                       ccTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             >  1:1511725/1‑140 (MQ=255)
                                       ccTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             >  1:3177986/1‑140 (MQ=255)
                                       ccTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             >  2:1552303/1‑140 (MQ=255)
                                       ccTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             >  2:607741/1‑140 (MQ=255)
                                       ccTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             <  1:1156564/140‑1 (MQ=255)
                                       ccTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             >  1:1359633/1‑140 (MQ=255)
                                       ccTTCCTGCAAAAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGt                                                                                             <  2:3488784/140‑1 (MQ=255)
                                          tCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTa                                                                                                                           >  1:264860/1‑107 (MQ=255)
                                          tCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGa                                                                                          >  1:1316390/1‑140 (MQ=255)
                                           ccTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGAc                                                                                         <  1:3297956/140‑1 (MQ=255)
                                            cttcACCAACTGACGTGGCTGCAATTAAAGCTACTCGTCCTGATACAAAGGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACg                                                                                        <  2:2901280/137‑1 (MQ=255)
                                               cACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGttc                                                                                     <  2:1084617/140‑1 (MQ=255)
                                                aCCCACTGACGGTGCTGCCACTCCAGCGACACGCCCTGCTACACATGACGCGATTGAGCCCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGttct                                                                                    <  2:2129856/140‑1 (MQ=255)
                                                aCCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGttct                                                                                    <  2:2568476/140‑1 (MQ=255)
                                                aCCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGttct                                                                                    <  2:3443955/140‑1 (MQ=255)
                                                aCCAACCGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGttct                                                                                    <  1:472182/140‑1 (MQ=255)
                                                 ccAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCAttt                                                                                                                            <  2:3287386/99‑1 (MQ=255)
                                                 ccAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCAttt                                                                                                                            <  2:757641/99‑1 (MQ=255)
                                                 ccAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGttctt                                                                                   >  1:3365230/1‑140 (MQ=255)
                                                  cAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGttcttc                                                                                  <  1:3604653/140‑1 (MQ=255)
                                                   aaCTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTcc                                                                                 >  1:2769560/1‑140 (MQ=255)
                                                   aaCTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTcc                                                                                 <  2:1007053/140‑1 (MQ=255)
                                                   aaCTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTcc                                                                                 <  2:3515037/140‑1 (MQ=255)
                                                        aCGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCGTTAATTTGCTAAGTTGACGTTCTTCCgtgtg                                                                            <  2:3342026/140‑1 (MQ=255)
                                                        aCGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCgtgtg                                                                            <  1:2037988/140‑1 (MQ=255)
                                                        aCGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCgtgtg                                                                            <  2:2394840/140‑1 (MQ=255)
                                                        aCGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCgtgtg                                                                            <  2:3156001/140‑1 (MQ=255)
                                                          gTTGCTGCAATTACAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGGTCTTCCGTGTGTa                                                                          <  1:1377096/140‑1 (MQ=255)
                                                          gTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTa                                                                          <  1:1142368/140‑1 (MQ=255)
                                                             gctgcAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTGCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTActc                                                                       >  1:739128/1‑140 (MQ=255)
                                                              ctgcAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTActct                                                                      >  1:991692/1‑140 (MQ=255)
                                                              ctgcAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTActct                                                                      >  2:2273044/1‑140 (MQ=255)
                                                                 cAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGa                                                                   <  1:3664959/140‑1 (MQ=255)
                                                                            aCTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAtgact                                                                                                                <  1:1028959/84‑1 (MQ=255)
                                                                             cTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTcc                                                                                 >  2:3580839/1‑114 (MQ=255)
                                                                             cTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTTTACTCTGGAACACTTGATTcc                                                       >  2:2684945/1‑140 (MQ=255)
                                                                              tCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTAtgactga                                                                                                              >  2:863288/1‑84 (MQ=255)
                                                                              tCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCg                                                      >  1:530174/1‑140 (MQ=255)
                                                                                  ccTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTc                                                  >  2:1494302/1‑140 (MQ=255)
                                                                                  ccTGATACAAATGAAGAGATTGAGCGCCATTATTCTGCTGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTACGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCTGTTc                                                  >  2:259524/1‑140 (MQ=255)
                                                                                     gATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCCCTGCAGTGGCATTTACACTTCGGCCTGACCCTGACGTTCGTAGTTTGACGTTCTTCCGTGTGTAGTGTGGAACACTTGATTCCTGGTCATa                                               >  1:1425069/1‑140 (MQ=255)
                                                                                     gATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGa                                                                   <  2:3014751/120‑1 (MQ=255)
                                                                                     gATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGa                                                                   >  1:3014751/1‑120 (MQ=255)
                                                                                          aaaTGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGa                                          >  1:3537961/1‑140 (MQ=255)
                                                                                           aaTGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGaa                                         <  1:2202167/140‑1 (MQ=255)
                                                                                            aTGACGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGaaa                                        >  1:1965077/1‑140 (MQ=255)
                                                                                            aTGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGaaa                                        >  1:3509874/1‑140 (MQ=255)
                                                                                            aTGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGaaa                                        >  1:3828928/1‑140 (MQ=255)
                                                                                            aTGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGaaa                                        >  2:3397356/1‑140 (MQ=255)
                                                                                                agagATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAAct                                    >  1:857676/1‑140 (MQ=255)
                                                                                                agagATTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAAct                                    <  2:295253/140‑1 (MQ=255)
                                                                                                    aTTGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTctac                                >  1:1122952/1‑140 (MQ=255)
                                                                                                     ttGAGCGCCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTctact                               <  2:2988779/140‑1 (MQ=255)
                                                                                                           gcCATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGGTATGACTGACCCTTACGTTGGTAAGTTGACGTACTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGc                         <  1:2884332/140‑1 (MQ=255)
                                                                                                            ccATTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCa                        <  2:249883/140‑1 (MQ=255)
                                                                                                              aTTCTTCTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACt                                                              >  2:498562/1‑100 (MQ=255)
                                                                                                               ttcttcTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCacac                     >  2:26341/1‑140 (MQ=255)
                                                                                                               ttcttcTGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAAc                     >  2:1586876/1‑140 (MQ=255)
                                                                                                                  ttctGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTg                  <  1:1482007/140‑1 (MQ=255)
                                                                                                                   tctGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTGACGTGGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGa                 <  2:1072852/140‑1 (MQ=255)
                                                                                                                    ctGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAg                <  2:132150/140‑1 (MQ=255)
                                                                                                                    ctGATGAAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAg                <  2:1392686/140‑1 (MQ=255)
                                                                                                                       atgaAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAgcgc             >  1:2359423/1‑140 (MQ=255)
                                                                                                                       atgaAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAgcgc             >  2:2195912/1‑140 (MQ=255)
                                                                                                                         gaAGAGCCATTCACTGCATTGGCATTTACAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAGCGCGt           <  1:2136870/140‑1 (MQ=255)
                                                                                                                         gaAGAGCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAGCGCGt           <  1:2481898/140‑1 (MQ=255)
                                                                                                                            gagCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAGCGCGTTgg        >  1:2276391/1‑140 (MQ=255)
                                                                                                                            gagCCATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGAAGTTCTTCCGTGTGTACTCTGGAACACTTTATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACTTGAGCGCGTTgg        >  2:3189285/1‑140 (MQ=255)
                                                                                                                               ccATTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAg                <  1:653744/129‑1 (MQ=255)
                                                                                                                                 aTTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTctact                               <  1:3222010/112‑1 (MQ=255)
                                                                                                                                 aTTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCa                        <  1:3454789/119‑1 (MQ=255)
                                                                                                                                 aTTCACTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAGCGCGTTGGACGTa   <  1:873177/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
CAAAGGTGTTCAACTAGTACTTGATGCTGTTCTTGACTACCTTCCTGCACCAACTGACGTTGCTGCAATTAAAGGTACTCGTCCTGATACAAATGAAGAGATTGAGCGCCATTCTTCTGATGAAGAGCCATTCTCTGCATTGGCATTTAAAGTTATGACTGACCCTTACGTTGGTAAGTTGACGTTCTTCCGTGTGTACTCTGGAACACTTGATTCCGGTTCATACGTGAAAAACTCTACTAAAGGCAAACGTGAGCGCGTTGGACGTAT  >  NZ_CP034943/131299‑131568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: