Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0930GC28.4% 170.5 / 18.6 105E172Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (32/41);  new base C (0/29);  total (33/72)
Fisher's exact test for biased strand distribution p-value = 2.32e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 112 total aligned reads displayed.

CGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAC  >  NZ_CP034943/1729954‑1730225
                                                                                                                                           |                                                                                                                                    
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                      >  1:849749/1‑140 (MQ=255)
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                      >  2:2448335/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                                    >  2:2081811/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                                    >  2:750130/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                                    >  1:1997083/1‑140 (MQ=255)
   aTCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaag                                                                                                                                   >  2:1307564/1‑140 (MQ=255)
          aaataaatTACCAAAATTTAAAGAAATTACTAAATACAAAAAGATTAAACGATAAAATATAATAAAAGAAACAGCTATTAAAAAGAGGCAGAAACTAAAAAAGAAACGAAATAAAAAGAAGATAAAAAAGAAGAAAGCGa                                                                                                                            <  2:2182278/140‑1 (MQ=255)
          aaaTCAATTACGAAAATTTAAAGAAATTACTAAATACAAAATCATTAAACCCTAAAAGATAATAAAAGCAAAAGCTATGAAAAACAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGa                                                                                                                            <  2:1734206/140‑1 (MQ=255)
             aaaaTCACTCAAAGTTAACGAAAGTAATAAACCCAAAAATAATAACCGATAAAACATAATCAAAGAAACAGATCTGAAAAAGACGAACACAATAAAAAAGAAACGAAAGAAAAAGAACAAACAAAACAACAACGCGACAg                                                                                                                         <  1:2876739/138‑1 (MQ=255)
                       aatgtcaaaTCAAGTACCAAATCCCAAAACATTAAACGCTAAAATATAATAAAAGCAACATCTCTGAAAAACAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGACGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                               <  2:1582038/134‑1 (MQ=255)
                       aaatttaaaGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAATACAAACAAACCAAAAGCTCTCAAAAACAGCCACAAACTAAAAAACAAACTAAACAAAAATAACATAAAAAACAAGAACGCGACAGTGATAAACTg                                                                                                               <  2:1265359/134‑1 (MQ=255)
                       aaaGTTAAATAAACTACCAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGAAACAGCTCTGAAAAACACTCACACACTAAAAAATAAACCAAACAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                               <  2:507368/140‑1 (MQ=255)
                               aGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTaa                                                                                                       >  2:1446646/1‑140 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                   >  1:1644174/1‑140 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                   >  1:2041219/1‑140 (MQ=255)
                                    gtaataaaTCCAAAAACATTCAACGCTAAAACAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACCAAACAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                                  <  2:849749/135‑1 (MQ=255)
                                     tACGAAATCCCAAAATATTAAACTCTAAAAGAGAAACAAAGCAACAGCTCTTAAAAAGAGGAAGACAATAAAAAACAACCCAAAGAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  2:1026834/140‑1 (MQ=255)
                                     tACGAAATCCCAAAAGATTCACCGATAAAAGATAATCAAAGCAACAGCTCTGAAAAAGAGGCACACACTAAAAAAGAAACGAAACAAAAATAAGATAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  2:2151616/140‑1 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAGAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  2:1911895/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATCCTAAAGAAGAAAGCGACCGTGATAAAGTGGAGATTAAAGCAAc                                                                                                 >  1:2128730/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  2:1591005/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:1167249/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:1513623/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:458763/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:1801682/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:880849/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:878652/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGATAAAAGAGAATAAAAGCAAAATCTCTGAAAAAGAGGCAGACACAAAAAAAGAAACTAAATAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  2:710735/140‑1 (MQ=255)
                                     tACGAAATCCCAAAACATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:2578320/1‑140 (MQ=255)
                                     cactaaaTCCAAAAATATTAAACCATAAAACAGAATCAACCCAACACCTCTTAAAAAGAGGCAGACACTAAAAAACAAACCAAACAAAAACAACAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  1:2727193/136‑1 (MQ=255)
                                       ctaaaaCCCAAAATATTCAACGCTAAAATATAATCAAAGCAACAGCTCTTAAAAATAGGCATACACTAAAAAACAAACGAAAGAAAAACAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                               <  2:1110251/138‑1 (MQ=255)
                                       ctaaaTCCAAAAAGATTAAACTCTAAAAGAGAATAAAAGAAACAGATCTTAAAAAGAGGCATAAAATAAAAAATAAACCAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                               <  2:2497011/138‑1 (MQ=255)
                                       ctaaaTCCAAAAAGATACAACGATAAAATAGAATCAAAGAAACAGCTCTTAAAAATAGGAAGAAACTAAAAAAGAAACCAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                               <  2:127326/138‑1 (MQ=255)
                                       ctaaaCCCAAAAAGATTCAACTAAAAAACAGAATCAAAGAAACAGCTCTAAAAAATAGGCACACAATAAAAAATAACCGAAACAAACACAACATAAAAAAGAAGACCGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                               <  1:2148618/138‑1 (MQ=255)
                                       ccaaaTCCAAAAATATCAACCGCTAAACCACAATCAAACCAACCCCTCTCAAAACGAGCAATAAAATAAAAAAAAACCCAAACAAAAACAATAAAAAAAACAAAAACGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                               <  1:2323005/138‑1 (MQ=255)
                                        taaaTACAAAAATATTCAACTATAAAAGAGAATAAAAGCAACAGATCTTAAAAAGAGGAATACACTAAAAAACAAACGAAACAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                              <  2:211577/139‑1 (MQ=255)
                                              ccAAAAGATTCAACGCTAAAAGATAAACAAAGCAACAGCTCTGAAAAAGAGGCAGAAACTAAAAAATAAACGAAAGAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                        <  2:2869128/140‑1 (MQ=255)
                                              ccAAAAGAATCAACCAAAAACCAGAAACAACCCAACCGCTCTCAAAAACAGCCACACACTAAAAAACAAACGAACCAAACACAACAAAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                        <  1:2192651/140‑1 (MQ=255)
                                               cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                       >  2:1558847/1‑140 (MQ=255)
                                               aaaaaGATTAAACGATAAAACACAAACAAAGCAACAGCTCTGAAAAAGAGCCACAAAAAAAAAAACAACCCAAACAAAAACAACATAAAAAACACCACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                       <  1:648418/139‑1 (MQ=255)
                                                  aaGATTCAACGATAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACACAAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                    <  2:1982941/140‑1 (MQ=255)
                                                       taaactatAAAATAGAATAAAAGCAAAAGATATTAAAAAGAGGCAGAAACTAAAAAAGAAACGAAAGAAAAAGAAAATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                               <  2:464308/133‑1 (MQ=255)
                                                       taaaCGATAAAACAGAAAAAAAGAAACAGCTCTTAAAAAGCGCCATAAAATAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                               <  2:169166/138‑1 (MQ=255)
                                                       tCAACGCTAAACGACAAACAAAGCCACCGCTCTGAAAAAGAGGCACACACTAAAAAACAAACCAAAGAAAAAGAACATAAAAAAGAACCAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                               <  2:459958/140‑1 (MQ=255)
                                                       aaaaCTCTAAAACACAATAAAAGCAAAACCAATTAAAAAGAGGCACACACAAAAAAATAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                               <  2:1619986/138‑1 (MQ=255)
                                                          actaaaaaaGAGAACAAAAGCAACAGCTCTGAAAAAGAGGCACACACTAAAAAACAAACCAACCAAAACGAACATAAAAACCAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:1986715/135‑1 (MQ=255)
                                                          accatAAAAGAGAATCAAACCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATACACGAAAGAAAAAGAAGAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:2870923/136‑1 (MQ=255)
                                                          accatAAAACACAATCAAACCAACCGCTCTTAAAAAGAGCCAGACACTAAAAAAGAAACCAAACAAAAACAACAAACAAAACCACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:141544/136‑1 (MQ=255)
                                                          accacaaaaGACAAAAAAACAAACACCTCTCAAAAACACGCAGACAAAAAAAAACAAACCAAACAAAAACAACATAAAAAACCACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:2378174/135‑1 (MQ=255)
                                                          aCGCTAAAATAGAATCAACGCAACAGATATTAAAAAGAGGCATACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  2:1447734/140‑1 (MQ=255)
                                                          aCGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  2:2652516/140‑1 (MQ=255)
                                                          aCGCTAAAAGACAATCAAAGCAACAGATATTAAAAAGAGGCACACACAAAAAAACAAACCAAACAAAAAGAAGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  2:1997083/140‑1 (MQ=255)
                                                          aCGCTAAAACACAATCAAAGAAACAGCTCTGAAAAAGAGCCACACAATAAAAAAGAAACCAAACAAAAAGAACATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:2285150/140‑1 (MQ=255)
                                                          aCGCTAAAACACAAAAAAACCAACACATCTTAAAAAGACGCAGACACTAAAAAACAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  2:2800571/140‑1 (MQ=255)
                                                          aCGCAAAACTAGACTCAACGCAACAGCTCTGAAAAAGAGGCACACAATAAAAAATAAACCAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  2:1082278/140‑1 (MQ=255)
                                                          aCGCAAAAACACAACCAAACCAACAGATATCAAAATCAGCCACACACAAAAAACCAAACCAAACAAAAACACCAAAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:1715931/140‑1 (MQ=255)
                                                          aCGATAAATGAAAATAAAAGCAACACTTCTGAAAAATCGGCAGACAATAAAAAATAAACCAAAGAAAAAGAACATAAAAACGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  1:2871154/140‑1 (MQ=255)
                                                          aCGATAAAATAGAATAAAAGCACCAGCTCTGAAAAAGAGTCAGAAACTAAAAAACAAACCAAACAAAACGAACATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGTTTAAAGCAACTGGCACTGAAGGGTCATTacc                                                                            <  2:443277/140‑3 (MQ=255)
                                                          aCGATAAAAGACAATAAAAGCAACAGCTATTAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAACAAGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                            <  2:2434545/140‑1 (MQ=255)
                                                             cTAAAACACAAACAAACCAACAGCTCTTAAAAAGAGGAACACAATAAAAAACAAACCAAACAAAAAGAACAAAAAAAACACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGAc                                                                         <  1:1292381/140‑1 (MQ=255)
                                                                  cgataaTCAAAGCAAAAGCTCTGAAAAAGAGGCAGACACTAACAAAGAAACGAACGAAAAACAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                    <  2:1406445/136‑1 (MQ=255)
                                                                  agatAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAACAAGATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                    <  2:2220040/140‑1 (MQ=255)
                                                                  agagAAACAACGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                    <  2:1406461/140‑1 (MQ=255)
                                                                    acaaTAAAACCAACCGATATCAAAAAGAGCCACACACAAAAAAACAAACCAAACAAAAACAACATAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                  <  1:728002/138‑1 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGACAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                >  1:1913309/1‑140 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                >  2:680735/1‑140 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                >  1:2789247/1‑140 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                >  2:2425097/1‑140 (MQ=255)
                                                                      aaTCAAACAAACCGAACTCAAAAAGAGCCAAAAAATAAAAAAAAACCAAAACAAAAACAACAAAAAACACCACACCGCGACAGTGATAAACTGGAGATTAACGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                <  1:476724/140‑1 (MQ=255)
                                                                        tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTTGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                              >  2:351587/1‑140 (MQ=255)
                                                                        tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                              >  2:1320569/1‑140 (MQ=255)
                                                                        tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                              >  2:351504/1‑140 (MQ=255)
                                                                         caacccAACAGATCTCAAAACGCGGCAGACACTAAAAACAAAACCAAACAAAAACACCAAAAAACCCACCCCCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACGGAAGGGTCATTAACGACTTATGAAGTGTc                                                             <  1:2448335/135‑1 (MQ=255)
                                                                         aaacccAACAGAACTTAAAACGAGCCACACAAAAAACAACACACCAACCAAAAACAAGAAACAAACCAAGCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                             <  1:1911895/135‑1 (MQ=255)
                                                                            aGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 >  1:2747451/1‑101 (MQ=255)
                                                                            aGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                 <  2:2747451/101‑1 (MQ=255)
                                                                              cAACAGATATTAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAACACGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGgcg                                                        <  2:1904635/140‑1 (MQ=255)
                                                                                    ctctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgaga                                                  >  1:218985/1‑140 (MQ=255)
                                                                                        cacacacagGCAGACACTAAAACACAAACGAAAGAACAAGAACATAAAAACGAACAAAGCGACAGTGAGAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAAtt                                              <  2:1955157/133‑1 (MQ=255)
                                                                                             agagCCAGACACTAAAAAACAAACGAAAGAAACACAAGATAAACAAGAAGAAAGCGACCGTGATAAACGGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                         <  2:2250666/140‑1 (MQ=255)
                                                                                              gagGCATAAACTAAAAAATAAACGAAAGAAAAATAAGATAAAAAAGCAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                        <  2:437786/140‑1 (MQ=255)
                                                                                              gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                        >  2:1251419/1‑140 (MQ=255)
                                                                                              gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                        >  1:2753990/1‑140 (MQ=255)
                                                                                                    gACACTAAAAAATAAACCAAAGAAAAATAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACt                                  <  2:2783652/140‑1 (MQ=255)
                                                                                                      cacTAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  2:2128730/140‑1 (MQ=255)
                                                                                                      caatAAAAAAGAAACGAAATCAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  2:2334449/137‑1 (MQ=255)
                                                                                                      aaatAAAAAACAAACGAAACAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  2:1801682/137‑1 (MQ=255)
                                                                                                      aaatAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAAGTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                <  2:2726948/137‑1 (MQ=255)
                                                                                                        caaaaaaaCAAACGAAAGAAAAATAACATAAAAACGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAg                              <  1:2028480/138‑1 (MQ=255)
                                                                                                        atAAAAAACAAACCAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAg                              <  1:1053289/139‑1 (MQ=255)
                                                                                                         tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg                             >  1:1080233/1‑140 (MQ=255)
                                                                                                           aaaaaGAAACGAAACAAAAATAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                           <  1:1682258/140‑1 (MQ=255)
                                                                                                                gAAACGAAAGAAAAAGAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  2:2585106/140‑1 (MQ=255)
                                                                                                                caaaCCAAACAAAATCAACATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                      <  1:848113/139‑1 (MQ=255)
                                                                                                                   aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAATGCATCAGACAg                   >  2:1491656/1‑140 (MQ=255)
                                                                                                                   aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                   >  2:2221819/1‑140 (MQ=255)
                                                                                                                   aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATGCGAGCTCGAACTGAAGGCATCAGACAg                   >  1:706407/1‑140 (MQ=255)
                                                                                                                           aaatgaaGATAAAAACGACGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGCAGTGTCCGGCTCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa           <  2:2432102/140‑1 (MQ=255)
                                                                                                                                    tAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAc  <  2:49763/140‑1 (MQ=255)
                                                                                                                                    tAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAc  <  2:1167249/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                    
CGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAC  >  NZ_CP034943/1729954‑1730225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑