Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0960GC25.2% 168.3 / 18.4 105E173Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (33/44);  new base C (0/26);  total (33/72)
Fisher's exact test for biased strand distribution p-value = 1.30e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

ATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGATGAAAA  >  NZ_CP034943/1729957‑1730235
                                                                                                                                           |                                                                                                                                           
aTCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaag                                                                                                                                             >  2:1307564/1‑140 (MQ=255)
       aaataaatTACCAAAATTTAAAGAAATTACTAAATACAAAAAGATTAAACGATAAAATATAATAAAAGAAACAGCTATTAAAAAGAGGCAGAAACTAAAAAAGAAACGAAATAAAAAGAAGATAAAAAAGAAGAAAGCGa                                                                                                                                      <  2:2182278/140‑1 (MQ=255)
       aaaTCAATTACGAAAATTTAAAGAAATTACTAAATACAAAATCATTAAACCCTAAAAGATAATAAAAGCAAAAGCTATGAAAAACAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGa                                                                                                                                      <  2:1734206/140‑1 (MQ=255)
          aaaaTCACTCAAAGTTAACGAAAGTAATAAACCCAAAAATAATAACCGATAAAACATAATCAAAGAAACAGATCTGAAAAAGACGAACACAATAAAAAAGAAACGAAAGAAAAAGAACAAACAAAACAACAACGCGACAg                                                                                                                                   <  1:2876739/138‑1 (MQ=255)
                    aatgtcaaaTCAAGTACCAAATCCCAAAACATTAAACGCTAAAATATAATAAAAGCAACATCTCTGAAAAACAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGACGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                                         <  2:1582038/134‑1 (MQ=255)
                    aaatttaaaGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAATACAAACAAACCAAAAGCTCTCAAAAACAGCCACAAACTAAAAAACAAACTAAACAAAAATAACATAAAAAACAAGAACGCGACAGTGATAAACTg                                                                                                                         <  2:1265359/134‑1 (MQ=255)
                    aaaGTTAAATAAACTACCAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGAAACAGCTCTGAAAAACACTCACACACTAAAAAATAAACCAAACAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                                                         <  2:507368/140‑1 (MQ=255)
                      aGTGAAAGCAAGTACGAAATCCCACAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAATAAGAAAGCGACAGTGATAAACTGga                                                                                                                       >  2:168061/1‑140 (MQ=255)
                            aGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTaa                                                                                                                 >  2:1446646/1‑140 (MQ=255)
                                aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                             >  1:1644174/1‑140 (MQ=255)
                                aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                             >  1:2041219/1‑140 (MQ=255)
                                 gtaataaaTCCAAAAACATTCAACGCTAAAACAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACCAAACAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                                            <  2:849749/135‑1 (MQ=255)
                                  tACGAAATCCCAAAATATTAAACTCTAAAAGAGAAACAAAGCAACAGCTCTTAAAAAGAGGAAGACAATAAAAAACAACCCAAAGAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           <  2:1026834/140‑1 (MQ=255)
                                  tACGAAATCCCAAAAGATTCACCGATAAAAGATAATCAAAGCAACAGCTCTGAAAAAGAGGCACACACTAAAAAAGAAACGAAACAAAAATAAGATAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           <  2:2151616/140‑1 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAGAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  2:1911895/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATCCTAAAGAAGAAAGCGACCGTGATAAAGTGGAGATTAAAGCAAc                                                                                                           >  1:2128730/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:458763/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:1513623/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:49763/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:878652/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:880849/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:1167249/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  2:1591005/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:1801682/1‑140 (MQ=255)
                                  tACGAAATCCCAAAAGATTCAACGATAAAAGAGAATAAAAGCAAAATCTCTGAAAAAGAGGCAGACACAAAAAAAGAAACTAAATAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           <  2:710735/140‑1 (MQ=255)
                                  tACGAAATCCCAAAACATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:2578320/1‑140 (MQ=255)
                                  tACGAAATCCAAAAAGATTCAACGCTAAAAGACAAACAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           <  2:1375522/140‑1 (MQ=255)
                                  cactaaaTCCAAAAATATTAAACCATAAAACAGAATCAACCCAACACCTCTTAAAAAGAGGCAGACACTAAAAAACAAACCAAACAAAAACAACAAAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           <  1:2727193/136‑1 (MQ=255)
                                    ctaaaaCCCAAAATATTCAACGCTAAAATATAATCAAAGCAACAGCTCTTAAAAATAGGCATACACTAAAAAACAAACGAAAGAAAAACAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                                         <  2:1110251/138‑1 (MQ=255)
                                    ctaaaTCCAAAAAGATTAAACTCTAAAAGAGAATAAAAGAAACAGATCTTAAAAAGAGGCATAAAATAAAAAATAAACCAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                                         <  2:2497011/138‑1 (MQ=255)
                                    ctaaaTCCAAAAAGATACAACGATAAAATAGAATCAAAGAAACAGCTCTTAAAAATAGGAAGAAACTAAAAAAGAAACCAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                                         <  2:127326/138‑1 (MQ=255)
                                    ctaaaCCCAAAAAGATTCAACTAAAAAACAGAATCAAAGAAACAGCTCTAAAAAATAGGCACACAATAAAAAATAACCGAAACAAACACAACATAAAAAAGAAGACCGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                                         <  1:2148618/138‑1 (MQ=255)
                                    ccaaaTCCCAAAAGATTCAACGCTAAAACAGAATCAAAGCACCAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAACAAGATAAAAAAGACGAAAGCGACAGTGATAAACGGGAGATTAAAGCAACTg                                                                                                         <  2:2286099/138‑1 (MQ=255)
                                    ccaaaTCCAAAAATATCAACCGCTAAACCACAATCAAACCAACCCCTCTCAAAACGAGCAATAAAATAAAAAAAAACCCAAACAAAAACAATAAAAAAAACAAAAACGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                                         <  1:2323005/138‑1 (MQ=255)
                                     taaaTACAAAAATATTCAACTATAAAAGAGAATAAAAGCAACAGATCTTAAAAAGAGGAATACACTAAAAAACAAACGAAACAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                                        <  2:211577/139‑1 (MQ=255)
                                           ccAAAAGAATCAACCAAAAACCAGAAACAACCCAACCGCTCTCAAAAACAGCCACACACTAAAAAACAAACGAACCAAACACAACAAAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                                  <  1:2192651/140‑1 (MQ=255)
                                            cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                                 >  2:1558847/1‑140 (MQ=255)
                                            cAAAAGATTCAACGATAAAAGATAATAAAAGCAACAGATATTAAAAAGAGGCAGACAATAAAAAACAAACGAAACAAAAAGAAGAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                                 <  1:2139897/140‑1 (MQ=255)
                                            aaaaaGATTAAACGATAAAACACAAACAAAGCAACAGCTCTGAAAAAGAGCCACAAAAAAAAAAACAACCCAAACAAAAACAACATAAAAAACACCACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                                 <  1:648418/139‑1 (MQ=255)
                                              aaaGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAgc                                                                                               >  2:2727193/1‑139 (MQ=255)
                                               aaGATTCAACGATAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACACAAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                              <  2:1982941/140‑1 (MQ=255)
                                                    taaactatAAAATAGAATAAAAGCAAAAGATATTAAAAAGAGGCAGAAACTAAAAAAGAAACGAAAGAAAAAGAAAATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                                         <  2:464308/133‑1 (MQ=255)
                                                    taaaCGATAAAACAGAAAAAAAGAAACAGCTCTTAAAAAGCGCCATAAAATAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                                         <  2:169166/138‑1 (MQ=255)
                                                    tCAACGCTAAACGACAAACAAAGCCACCGCTCTGAAAAAGAGGCACACACTAAAAAACAAACCAAAGAAAAAGAACATAAAAAAGAACCAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                                         <  2:459958/140‑1 (MQ=255)
                                                    aaaaCTCTAAAACACAATAAAAGCAAAACCAATTAAAAAGAGGCACACACAAAAAAATAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                                         <  2:1619986/138‑1 (MQ=255)
                                                       accatAAAAGAGAATCAAACCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATACACGAAAGAAAAAGAAGAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  1:2870923/136‑1 (MQ=255)
                                                       accatAAAACACAATCAAACCAACCGCTCTTAAAAAGAGCCAGACACTAAAAAAGAAACCAAACAAAAACAACAAACAAAACCACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  1:141544/136‑1 (MQ=255)
                                                       accacaaaaGACAAAAAAACAAACACCTCTCAAAAACACGCAGACAAAAAAAAACAAACCAAACAAAAACAACATAAAAAACCACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  1:2378174/135‑1 (MQ=255)
                                                       aCGCTAAAATAGAATCAACGCAACAGATATTAAAAAGAGGCATACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  2:1447734/140‑1 (MQ=255)
                                                       aCGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  2:2652516/140‑1 (MQ=255)
                                                       aCGCTAAAAGACAATCAAAGCAACAGATATTAAAAAGAGGCACACACAAAAAAACAAACCAAACAAAAAGAAGATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  2:1997083/140‑1 (MQ=255)
                                                       aCGCTAAAACACAATCAAAGAAACAGCTCTGAAAAAGAGCCACACAATAAAAAAGAAACCAAACAAAAAGAACATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  1:2285150/140‑1 (MQ=255)
                                                       aCGCTAAAACACAAAAAAACCAACACATCTTAAAAAGACGCAGACACTAAAAAACAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  2:2800571/140‑1 (MQ=255)
                                                       aCGCAAAACTAGACTCAACGCAACAGCTCTGAAAAAGAGGCACACAATAAAAAATAAACCAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  2:1082278/140‑1 (MQ=255)
                                                       aCGCAAAAACACAACCAAACCAACAGATATCAAAATCAGCCACACACAAAAAACCAAACCAAACAAAAACACCAAAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  1:1715931/140‑1 (MQ=255)
                                                       aCGATAAATGAAAATAAAAGCAACACTTCTGAAAAATCGGCAGACAATAAAAAATAAACCAAAGAAAAAGAACATAAAAACGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  1:2871154/140‑1 (MQ=255)
                                                       aCGATAAAATAGAATAAAAGCACCAGCTCTGAAAAAGAGTCAGAAACTAAAAAACAAACCAAACAAAACGAACATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGTTTAAAGCAACTGGCACTGAAGGGTCATTacc                                                                                      <  2:443277/140‑3 (MQ=255)
                                                       aCGATAAAAGACAATAAAAGCAACAGCTATTAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAACAAGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  2:2434545/140‑1 (MQ=255)
                                                       aCGATAAAACAGAATCAAACCAACAGCTCTAAAAAAGAGCCACACACAAAAAAATAAACCAAACAAAAACAACATAAAAAACACCCCCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                      <  1:1716165/140‑1 (MQ=255)
                                                          cTAAAACACAAACAAACCAACAGCTCTTAAAAAGAGGAACACAATAAAAAACAAACCAAACAAAAAGAACAAAAAAAACACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGAc                                                                                   <  1:1292381/140‑1 (MQ=255)
                                                               agatAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAACAAGATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                              <  2:2220040/140‑1 (MQ=255)
                                                               agagAAACAACGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                              <  2:1406461/140‑1 (MQ=255)
                                                                 acaaTAAAACCAACCGATATCAAAAAGAGCCACACACAAAAAAACAAACCAAACAAAAACAACATAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                            <  1:728002/138‑1 (MQ=255)
                                                                   aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGACAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                          >  1:1913309/1‑140 (MQ=255)
                                                                   aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                          >  2:680735/1‑140 (MQ=255)
                                                                   aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                          >  1:2789247/1‑140 (MQ=255)
                                                                   aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                          >  2:2425097/1‑140 (MQ=255)
                                                                   aaTCAAACAAACCGAACTCAAAAAGAGCCAAAAAATAAAAAAAAACCAAAACAAAAACAACAAAAAACACCACACCGCGACAGTGATAAACTGGAGATTAACGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                          <  1:476724/140‑1 (MQ=255)
                                                                     tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTTGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                        >  2:351587/1‑140 (MQ=255)
                                                                     tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                        >  2:351504/1‑140 (MQ=255)
                                                                     tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                        >  2:1320569/1‑140 (MQ=255)
                                                                      caacccAACAGATCTCAAAACGCGGCAGACACTAAAAACAAAACCAAACAAAAACACCAAAAAACCCACCCCCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACGGAAGGGTCATTAACGACTTATGAAGTGTc                                                                       <  1:2448335/135‑1 (MQ=255)
                                                                      aaacccAACAGAACTTAAAACGAGCCACACAAAAAACAACACACCAACCAAAAACAAGAAACAAACCAAGCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                       <  1:1911895/135‑1 (MQ=255)
                                                                         aGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           >  1:2747451/1‑101 (MQ=255)
                                                                         aGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                           <  2:2747451/101‑1 (MQ=255)
                                                                                 ctctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgaga                                                            >  1:218985/1‑140 (MQ=255)
                                                                                     cacacacagGCAGACACTAAAACACAAACGAAAGAACAAGAACATAAAAACGAACAAAGCGACAGTGAGAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAAtt                                                        <  2:1955157/133‑1 (MQ=255)
                                                                                           gagGCATAAACTAAAAAATAAACGAAAGAAAAATAAGATAAAAAAGCAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                                  <  2:437786/140‑1 (MQ=255)
                                                                                           gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                                  >  2:1251419/1‑140 (MQ=255)
                                                                                           gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                                  >  1:2753990/1‑140 (MQ=255)
                                                                                                 gACACTAAAAAATAAACCAAAGAAAAATAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACt                                            <  2:2783652/140‑1 (MQ=255)
                                                                                                   cacTAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                          <  2:2128730/140‑1 (MQ=255)
                                                                                                   caatAAAAAAGAAACGAAATCAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                          <  2:2334449/137‑1 (MQ=255)
                                                                                                   aacTAAAAAATAAACTAAACAAAAAGAACATAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                          <  2:458763/139‑1 (MQ=255)
                                                                                                   aaatAAAAAACAAACGAAACAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                          <  2:1801682/137‑1 (MQ=255)
                                                                                                   aaatAAAAAACAAACGAAACAAAAAGAAGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAAGTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                          <  2:2726948/137‑1 (MQ=255)
                                                                                                     caaaaaaaCAAACGAAAGAAAAATAACATAAAAACGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAg                                        <  1:2028480/138‑1 (MQ=255)
                                                                                                     atAAAAAACAAACCAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAg                                        <  1:1053289/139‑1 (MQ=255)
                                                                                                      tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg                                       >  1:1080233/1‑140 (MQ=255)
                                                                                                        aaaaaGAAACGAAACAAAAATAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                                     <  1:1682258/140‑1 (MQ=255)
                                                                                                             gAAACGAAAGAAAAAGAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                                <  2:2585106/140‑1 (MQ=255)
                                                                                                              aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAAGACTTATTAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTCAAGGCATCAGaa                               >  2:200939/1‑139 (MQ=255)
                                                                                                                aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAATGCATCAGACAg                             >  2:1491656/1‑140 (MQ=255)
                                                                                                                aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                             >  2:2221819/1‑140 (MQ=255)
                                                                                                                aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATGCGAGCTCGAACTGAAGGCATCAGACAg                             >  1:706407/1‑140 (MQ=255)
                                                                                                                        aaatgaaGATAAAAACGACGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGCAGTGTCCGGCTCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa                     <  2:2432102/140‑1 (MQ=255)
                                                                                                                                 tAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAc            <  2:49763/140‑1 (MQ=255)
                                                                                                                                 tAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTAc            <  2:1167249/140‑1 (MQ=255)
                                                                                                                                      aacatgaaAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACTACTTATGAAGTGTCCGGCTCTGAGAACTTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGAt       <  2:1540511/135‑1 (MQ=255)
                                                                                                                                           gaaAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGATGaaaa  >  2:2173476/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGATGAAAA  >  NZ_CP034943/1729957‑1730235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑