Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 44,881 G→A intergenic (‑62/‑439) abrB ← / → metG transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494344,8810GA99.2% 460.8 / ‑5.2 123intergenic (‑62/‑439)abrB/metGtransition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (57/65);  minor base T (0/1);  total (57/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 123 total aligned reads displayed.

AGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTT  >  NZ_CP034943/44745‑45009
                                                                                                                                        |                                                                                                                                
actACGACGCACTTCGATAGCAATAACTACACGTCCTAGCTCATCAACTTTCCGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACa                                                                                                                               <  1:1588066/138‑1 (MQ=255)
       cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                        <  1:931740/140‑1 (MQ=255)
       cgCAATTCGATAGGAATAACTACACGTCATAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                        <  2:2466768/140‑1 (MQ=255)
        gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCtt                                                                                                                       <  1:1150175/140‑1 (MQ=255)
         cAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATAACAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTa                                                                                                                      <  2:783216/140‑1 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                     <  2:194787/140‑1 (MQ=255)
          aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                     >  2:2478596/1‑140 (MQ=255)
          aaTTCGATAGGAATAACGACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTAc                                                                                                                     <  2:1367691/140‑1 (MQ=255)
                aTAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCAt                                                                                                               <  1:54688/140‑1 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                              >  1:188439/1‑140 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                              <  1:2478596/140‑1 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                              <  1:2751395/140‑1 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                              >  1:1248458/1‑140 (MQ=255)
                 tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATa                                                                                                              >  1:949888/1‑140 (MQ=255)
                    gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCa                                                                                                           <  1:2009903/140‑1 (MQ=255)
                    gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCa                                                                                                           >  2:916016/1‑140 (MQ=255)
                    gAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCa                                                                                                           >  1:1016146/1‑140 (MQ=255)
                      aTAACTACACGTCCTAGCTCATCAACTTTACTTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTATTCGTCATTATTGAACAAAATCCTTACCTTCATAGCATa                                                                                                         >  2:496460/1‑140 (MQ=255)
                           tACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAg                                                                                                    <  2:2149985/140‑1 (MQ=255)
                            acacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAgg                                                                                                   <  2:1248458/140‑1 (MQ=255)
                            acacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAgg                                                                                                   <  2:188439/140‑1 (MQ=255)
                             cacGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                        >  1:2079283/1‑118 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                 >  1:2700045/1‑140 (MQ=255)
                              acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                                 >  1:1645845/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:2066512/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:695295/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:2353074/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:579252/1‑140 (MQ=255)
                               cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                                >  2:808993/1‑140 (MQ=255)
                                  ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                             >  2:2235032/1‑140 (MQ=255)
                                  ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                             >  1:1334181/1‑140 (MQ=255)
                                  ccTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCa                                                                                             >  2:661681/1‑140 (MQ=255)
                                   cTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCaa                                                                                            >  1:918821/1‑140 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                        <  1:1319566/105‑1 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCt                                                                                                                        >  2:1319566/1‑105 (MQ=255)
                                          atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGt                                                                                     <  1:1387600/140‑1 (MQ=255)
                                           tcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAATAAGGTTTCCAATAATCGTc                                                                                    >  2:1226957/1‑140 (MQ=255)
                                           tcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTc                                                                                    >  2:2045223/1‑140 (MQ=255)
                                            caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGAATAACAAGCTTTCCAATAATCGTCa                                                                                   >  2:2518676/1‑140 (MQ=255)
                                            caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCATTACCTTCATAGCATAACAAGTTTTCCAATAATCGTCa                                                                                   >  2:2125126/1‑140 (MQ=255)
                                                  tACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtt                                                                             <  2:463877/140‑1 (MQ=255)
                                                  tACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtt                                                                             >  2:1041855/1‑140 (MQ=255)
                                                    cGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtttt                                                                           <  1:1226957/140‑1 (MQ=255)
                                                      tACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgt                                                                         <  2:918821/140‑1 (MQ=255)
                                                        ccagaCCAGTAGATTTCATCAACTTTGTCCTCCCAAGAGATGATTATTTGTTTAAATAATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATACTCGTCAATCATtttgttt                                                                       <  2:1958524/136‑1 (MQ=255)
                                                          aTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGc                                                                     >  1:2604347/1‑140 (MQ=255)
                                                          aTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGc                                                                     >  2:2718841/1‑140 (MQ=255)
                                                          aTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTAAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGc                                                                     >  2:780978/1‑140 (MQ=255)
                                                            aCCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTa                                                                   <  2:1233764/140‑1 (MQ=255)
                                                              cAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTatt                                                                 >  1:1826760/1‑140 (MQ=255)
                                                                gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                               >  1:266363/1‑140 (MQ=255)
                                                                 tAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgat                                                              >  1:1156463/1‑140 (MQ=255)
                                                                 tAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgat                                                              >  1:1852964/1‑140 (MQ=255)
                                                                  aGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgatt                                                             <  2:1334181/140‑1 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTTTTTGTTTAAATTATATTTTTCTTCGTCATTATTCACCAAAATCCTTACCTTCATAGCAGAACAAGGTTTCCACGAATCGTCAATGATTTTGTTTGCTATTGATTGTa                                                          <  1:1283836/140‑1 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          >  2:2527476/1‑140 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          <  1:2527476/140‑1 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          <  2:630380/140‑1 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          >  2:640258/1‑140 (MQ=255)
                                                                     tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                          <  1:640258/140‑1 (MQ=255)
                                                                      ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                                         >  2:890716/1‑140 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  1:916016/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  2:362056/140‑1 (MQ=255)
                                                                           aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                                    <  1:2066512/140‑1 (MQ=255)
                                                                            aaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTa                                                   <  1:890716/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACCAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                  <  1:1033388/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                  <  1:1517980/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                  <  1:2353074/140‑1 (MQ=255)
                                                                             aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                                  <  1:710634/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:210617/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:949888/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:2717942/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:1156463/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:1179229/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:1826760/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:209517/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:1939847/140‑1 (MQ=255)
                                                                                  ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                             <  2:1702736/140‑1 (MQ=255)
                                                                                   tcctccCACGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                            <  1:1556961/140‑1 (MQ=255)
                                                                                   tcctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                            <  2:519730/140‑1 (MQ=255)
                                                                                    cctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                           <  1:1934328/140‑1 (MQ=255)
                                                                                    cctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtat                                           <  2:221058/140‑1 (MQ=255)
                                                                                     ctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCACCAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                                          <  2:79949/140‑1 (MQ=255)
                                                                                     ctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                                          <  1:448111/140‑1 (MQ=255)
                                                                                     ctccCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                                          <  1:2045223/140‑1 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                        <  2:1039774/140‑1 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                        <  1:2235032/140‑1 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                        <  2:2700045/140‑1 (MQ=255)
                                                                                       cccAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTAATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                                        <  2:2465134/140‑1 (MQ=255)
                                                                                       acatagatATGTTTATTTGTTTATATTATATTTTTCTTGGTCATTTTTATAAATAATCATTTCCTTCATAGCATAACTACGTTTAAAATAATCCTAAATCATTTTGTTTGCTATTGATTGTTAGAATTAGTTATTGTAGt                                        <  1:661681/136‑1 (MQ=255)
                                                                                            gagaTGATTATTTCTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATACCATAACAAGGTTTCCAATACTCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATACTCAGTa                                   <  2:1730078/140‑1 (MQ=255)
                                                                                             agaTGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATGATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTaa                                  >  1:1389983/1‑140 (MQ=255)
                                                                                                  attattTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACACGGTTTCCAATAATCGTCAATCATTTTGGTTGGTATTGATTGTAAGAATTAGTGCTTATAGTCAGTAATGTag                             >  2:2389044/1‑139 (MQ=255)
                                                                                                  attattTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTAt                             >  1:1244181/1‑140 (MQ=255)
                                                                                                  attattTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTAt                             >  2:132209/1‑140 (MQ=255)
                                                                                                    tattTGTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCa                           >  2:534218/1‑140 (MQ=255)
                                                                                                         gTTTAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAac                      >  2:1117429/1‑140 (MQ=255)
                                                                                                            tAAATTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactac                   >  1:1402624/1‑140 (MQ=255)
                                                                                                             aaaTTATATTTTTCTTCGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactact                  <  1:1041855/140‑1 (MQ=255)
                                                                                                                             cGTCATTATTCAACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCttttt  >  1:2166801/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                
AGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGACAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAACAGCTTTTT  >  NZ_CP034943/44745‑45009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: