Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,078 C→G 9.4% E565Q (GAA→CAA)  EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0780CG 9.4% 521.2 / 3.4 215E565Q (GAA→CAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (32/144);  new base G (20/2);  total (69/146)
Fisher's exact test for biased strand distribution p-value = 1.53e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

ATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATA  >  NZ_CP034943/633941‑634217
                                                                                                                                         |                                                                                                                                           
aTGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttcttctt                                                                                                                                           <  2:77777/140‑1 (MQ=255)
aTGGTCTTGATAGAGTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttcttctt                                                                                                                                           <  2:3934886/140‑1 (MQ=255)
      ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                                                     <  1:1314572/140‑1 (MQ=255)
      ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                                                     <  2:1816255/140‑1 (MQ=255)
      ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                                                     <  1:1999847/140‑1 (MQ=255)
       tGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGa                                                                                                                                    <  2:503452/140‑1 (MQ=255)
        gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTGGTTGTTTTTCAATTCGTGTTGTTTTTAATATTaa                                                                                                                                   >  1:3135961/1‑140 (MQ=255)
              tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGtttt                                                                                                                             >  2:128594/1‑140 (MQ=255)
              tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCATTCTTTTTATTATTAATATGTACGtttt                                                                                                                             >  2:3492477/1‑140 (MQ=255)
                  cGGATCAGATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                         <  1:2324512/140‑1 (MQ=255)
                  cGGATCACATATCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                         <  2:2252715/140‑1 (MQ=255)
                  cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                         <  1:539254/140‑1 (MQ=255)
                  cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                         <  1:3362582/140‑1 (MQ=255)
                  cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                         <  2:3603806/140‑1 (MQ=255)
                  cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                         <  2:791234/140‑1 (MQ=255)
                  cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGGGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                         <  1:3601018/140‑1 (MQ=255)
                       cacaTAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGc                                                                                                                    <  2:1714303/140‑1 (MQ=255)
                       cacaTAGCAATTCATCGTTATGACTAATGTTTTCTTCAAGGGTGTGAACAGTGGCTTCAATTTCTTCCATCCAACGGAGTCCTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGc                                                                                                                    <  2:2036202/140‑1 (MQ=255)
                                   cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTCTTTTTGCATTGTTTTTCTTATTAATATGAAAGTTTTTAGTCAGGCTTAAACGAAGCa                                                                                                        >  2:3211071/1‑140 (MQ=255)
                                   cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTACATTCTTTTTCTTCTTCATATTAACGTTTTGAGTCAGGCTTATACGAAGCa                                                                                                        >  2:1956047/1‑140 (MQ=255)
                                   cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTACATTGTTTTTATTATTAATAATAACGTTTTTATTACGGCTTAACCGAAGCa                                                                                                        >  2:2556014/1‑140 (MQ=255)
                                    aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTGTTTTTCCATTGTTTTTCTTCTTCATATGAACGTTTTGAGTGAGGCTTAACCGAATCAg                                                                                                       >  2:3656705/1‑140 (MQ=255)
                                    aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTCCATTGTTTTTGTTGTTCATATGAACGTTTTTAGTCAGGCTTAAACGAAGCAg                                                                                                       >  2:2402528/1‑140 (MQ=255)
                                    aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGGTTCGTTTTGCAGTCTTTTTGTGATTGAGATTCACGGTTTTTGTGAGGCTTAAACGAAGccg                                                                                                       >  1:2110180/1‑138 (MQ=255)
                                      cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGGTTTTGGATTGTTTTTTGTGGTGATATGGACGGTTTGTTTGAGGGGTAACCGAAGCAggg                                                                                                     >  1:35307/1‑139 (MQ=255)
                                        ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGGCTTTGTTTTTTCAGTGTTTTTTTTCTTAAAATTAACGTTTTGTGTCAGGCTTTAACGAAGCAGGCgg                                                                                                   >  1:3772888/1‑139 (MQ=255)
                                        ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGGCTTTGTTTTTGCCTTATTTTTATTGTTGATATGTAAGTTTTTAGTGAAGCTTTAACCAAGCAGGCgg                                                                                                   >  1:533753/1‑139 (MQ=255)
                                        ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGTTTTTGCGGTGGTTTTGGTGGTGATATGAACGTGTTTGTTGAGGGGTAAACGAAGCAggggg                                                                                                   >  1:3442522/1‑137 (MQ=255)
                                             aCTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGtttttt                                                                                              <  2:2689524/140‑1 (MQ=255)
                                                 aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                                                          <  1:903446/140‑1 (MQ=255)
                                                  tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTTAGTCACGCTTAACCGAAGCAGGCGTTTTTTAGGag                                                                                         >  2:1685037/1‑139 (MQ=255)
                                                  tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTACATTATTTTTCTTATTAAAATTAAAGTTTTTATTAAGGCTTAAACGAAGCAGGCGTTTTTTAGGAt                                                                                         >  2:1053571/1‑140 (MQ=255)
                                                      ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGGTTTTTTTTGGATTGTTTTTTTTGTTGATATGAAAGTTTTTTGTCAGGGGTTAACGGAGAAGGGGTTTTTTTGGttttgt                                                                                     >  1:3701442/1‑137 (MQ=255)
                                                       tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTGTGTTTTTGGCATATTTTTATTTTTGATATTGACGGTTTGTGTGAGGCTTAAACGAATCACGCGTTTTTTGGGtttgcgg                                                                                    >  1:427601/1‑136 (MQ=255)
                                                       tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGGTGTTTGTTTTTTCCATCTTTTTTTTGTTGATATTAAAGTTTTTGTTGAGGCTTTAAAGAACCAGGCCTTTTTTTGGGttgcgg                                                                                    >  1:310976/1‑136 (MQ=255)
                                                                   tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGGGGTGGTTGGTTTTTCATTGGTTTTTGTCTTGATATGTAAGTTTTTTTTGAGGGGTAAACCGAGAAGGCGGTTTTTTGGAATGCTCTTTCttatttat                                                                        >  1:2087151/1‑140 (MQ=255)
                                                                   tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAt                                                                        <  2:275538/140‑1 (MQ=255)
                                                                    gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                                                       <  1:1053571/140‑1 (MQ=255)
                                                                    gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                                                       <  2:2670459/140‑1 (MQ=255)
                                                                    gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                                                       <  1:2741792/140‑1 (MQ=255)
                                                                        aGTTGCTTCAATTTCTTCAATCCGACGGAGTAGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTTCTGATTCATTTtct                                                                   <  1:1007255/140‑1 (MQ=255)
                                                                        aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                                                   <  1:1553051/140‑1 (MQ=255)
                                                                        aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTCTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                                                   <  1:1417794/140‑1 (MQ=255)
                                                                         gTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtctc                                                                  <  1:2927722/140‑1 (MQ=255)
                                                                          tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTGTTTTTGCCATGTTTTTCTTGTTGATATGAACGGTTTTGGTCAGGGGTTACCGAAGGAGGCGTTTTTTGGGATTGCGCTTGGTGTTTAATTTTGTcc                                                                 >  2:621446/1‑140 (MQ=255)
                                                                          tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTGGTTTCTTTTTGCATTGTTTTTGTTGTTGATATTGACGTTTTTAGTGAGGCTTAACCGAAGCAGGCGTTTTTTGGGCTTACGCTTGCTGATTAAATTTGTcc                                                                 >  1:814443/1‑140 (MQ=255)
                                                                          tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCATTGTTTTTGTTCTTGATATGTACGTTTTTTGTCAGGCTTTAACGAAGCCGGCGTTTTTTTGGATTACTGGTGCTGATTAAttttgtac                                                                 >  2:1399985/1‑136 (MQ=255)
                                                                          tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTATTTTTGCATTCTTTTTGTTGTTCATATGTACGTTTTGAGTCAGGCTTTACCGAAGCAGGCGTTTTTTGGGATTGCTCTTGCTGATTAATTTTCTcc                                                                 >  2:643470/1‑140 (MQ=255)
                                                                          tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTGTTTTTTCAGTGTTTTTGGTCTTGATATGTACCTTTTTTGTCATGCTTAACCCAAACAGGCGTTTTTTGGGGTTGCTGTTTCTGATTGATTTTATcc                                                                 >  2:1496332/1‑140 (MQ=255)
                                                                          tGGCTTCAATTTCTTCAATCCGACGGAGTCGATGGCGGTCGTTCTTTTTGCAATATTTTTAATATTGAAATAAACCTTTTTATTAAGGGTTAAAGCAAGCAGGCGTTTTTTAGCATTAATCATGAAGATTCAttttatac                                                                 >  2:473430/1‑136 (MQ=255)
                                                                                cAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGATTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCa                                                           <  2:240379/140‑1 (MQ=255)
                                                                                  aTTTCTACAAGCCGACGGAGTCGTGGGCGTTCTATCTTTTTCCATTCTTTTTCGTCGTCCTAGGAACGTTTTGAGTCAGGCTTCACCGAACCAGGCGTTTTTTCGGATTCCACGTGCTGATGCATTTTCTCCAATACaac                                                         <  1:1739870/140‑2 (MQ=255)
                                                                                     tcttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctg                                                      <  2:1163106/140‑1 (MQ=255)
                                                                                      cttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgc                                                     <  2:1537943/140‑1 (MQ=255)
                                                                                      cttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgc                                                     <  2:203/140‑1 (MQ=255)
                                                                                       ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                    <  2:55435/140‑1 (MQ=255)
                                                                                       ttcAATCCGACGGAGTCGTTGGCGTTCTGTCTTTTTCCATTCTTGTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                    <  2:3632926/140‑1 (MQ=255)
                                                                                       gtcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCCTCTGAACGTTTTGAGGCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                    <  2:2723440/139‑1 (MQ=255)
                                                                                                 cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                          <  1:2170557/140‑1 (MQ=255)
                                                                                                 cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                          <  1:2360807/140‑1 (MQ=255)
                                                                                                 cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                          <  2:694243/140‑1 (MQ=255)
                                                                                                 cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTGTTCTTCTTCATATGACCGTTTTGCGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                          <  1:3153011/140‑1 (MQ=255)
                                                                                                   gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATCTGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                        <  2:1631583/140‑1 (MQ=255)
                                                                                                   gAGTCGTTGGCGTTCTTTCTTGTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                        <  2:3135961/140‑1 (MQ=255)
                                                                                                      tCGTTGGCTTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                     <  2:1121993/140‑1 (MQ=255)
                                                                                                      tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                     <  2:178058/140‑1 (MQ=255)
                                                                                                           ggCGTTCGTTCTTGTTTCATTCTATTTCTTGTTGAGATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTGGTATTCCTCTTGCTGATTCATTTTCTCCTTTTCAAGCTTCTCTGTTTTTTTCTCCGTataa                                <  2:3692996/140‑1 (MQ=255)
                                                                                                              gTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATAAGAACGTTTTTAGTCACGCTTAAACGAAGCAGGCGTTTTTTCGGATTCCTATTGCAGATTAATTTTCTCCAATTAAAGCAGCTCTGTTTTTTTCTACGTAtaataa                             >  2:3327699/1‑140 (MQ=255)
                                                                                                                tctttcttTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATc                           >  2:1478962/1‑140 (MQ=255)
                                                                                                                tctttcttTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATc                           >  2:3860903/1‑140 (MQ=255)
                                                                                                                 ctttctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCg                          <  1:936331/140‑1 (MQ=255)
                                                                                                                   ttctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTa                        <  1:1458650/140‑1 (MQ=255)
                                                                                                                   ttctttTTCCATTCTTTTTCTTCTTCATATGAACGATTTGAGTCAGGCTGAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTa                        <  2:1923870/140‑1 (MQ=255)
                                                                                                                    tctttTTCCATTCTTTTTCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTATTCATGATTCATTATCTCCAATTAAACCTGCTCTTTTTTTTTCTCCGTATAATAATCTTaa                       >  2:3446477/1‑140 (MQ=255)
                                                                                                                    tctttTTCCATTCTTTTTCGTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCGTGCGGATGCATTTTCTCCAATTCAAGCTGCTCTGTTGTTTTGTCCGTCTACTAATCGtca                       >  2:1074699/1‑138 (MQ=255)
                                                                                                                     ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                      <  1:2522389/140‑1 (MQ=255)
                                                                                                                     ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                      <  2:2933446/140‑1 (MQ=255)
                                                                                                                     ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                      <  2:3044686/140‑1 (MQ=255)
                                                                                                                     ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATACATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                      <  2:214374/140‑1 (MQ=255)
                                                                                                                          tCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTccc                 <  1:2286859/140‑1 (MQ=255)
                                                                                                                             aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa              <  2:3822177/140‑1 (MQ=255)
                                                                                                                              ttCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGAt             <  1:3931077/140‑1 (MQ=255)
                                                                                                                              ttCTTTTTCGTCTTCATCTGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGAt             <  1:3930058/140‑1 (MQ=255)
                                                                                                                               tatttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  2:3251384/138‑1 (MQ=255)
                                                                                                                               tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  2:2228953/140‑1 (MQ=255)
                                                                                                                               tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  2:2511590/140‑1 (MQ=255)
                                                                                                                               tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  1:1496332/140‑1 (MQ=255)
                                                                                                                               tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  1:2465344/140‑1 (MQ=255)
                                                                                                                               tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  1:2772303/140‑1 (MQ=255)
                                                                                                                               tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  1:2628985/140‑1 (MQ=255)
                                                                                                                               tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa            <  2:2217288/140‑1 (MQ=255)
                                                                                                                                 tttttCTTCTTCCTATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct          <  2:2593999/140‑1 (MQ=255)
                                                                                                                                 tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct          <  1:128594/140‑1 (MQ=255)
                                                                                                                                 tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct          <  2:1443787/140‑1 (MQ=255)
                                                                                                                                 tatttCTGCGTCATATGAACGTTTTGAGTCCGGGTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCGTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct          <  1:2394262/138‑1 (MQ=255)
                                                                                                                                   tttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATActct        <  2:2374104/140‑1 (MQ=255)
                                                                                                                                    ttcttcttaTTATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCTTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACtctt       <  2:3537724/140‑1 (MQ=255)
                                                                                                                                        tcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat   <  1:3860903/140‑1 (MQ=255)
                                                                                                                                        tcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat   <  1:594269/140‑1 (MQ=255)
                                                                                                                                         cttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGtata                                 >  2:874772/1‑109 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATA  >  NZ_CP034943/633941‑634217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: