Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,152,044 G→T 8.1% D53E (GAC→GAA EO946_RS05920 ← GNAT family N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,152,0440GT 8.1% 529.0 / 4.5 214D53E (GAC→GAAEO946_RS05920GNAT family N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (87/100);  new base T (24/2);  total (112/102)
Fisher's exact test for biased strand distribution p-value = 4.77e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

GTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCAAT  >  NZ_CP034943/1151907‑1152181
                                                                                                                                         |                                                                                                                                         
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAAGCCGCTGCCGGAAATAATGtc                                                                                                                                         >  2:1347284/1‑140 (MQ=255)
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAAGCCGATGCAGTAAATAATgac                                                                                                                                         >  2:3673931/1‑138 (MQ=255)
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGAAGACGAGTCCGATATAGGCAAGAAGGCCAAACAAAAAAAAGCCGATGCAGGAAATAATGtc                                                                                                                                         >  2:1633542/1‑140 (MQ=255)
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCGTTGTCTACGATGACGACTCCGAGAGAGGCAATACCGCCAAACAAAAAAAAGCCGATTCCGGAAATAATGtc                                                                                                                                         >  1:1630491/1‑140 (MQ=255)
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGGATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACCAAAACAAGCCGCTGCAGGAAATAATGtg                                                                                                                                         >  2:2490183/1‑139 (MQ=255)
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTTTATTATTTGTCTACGATGACGAGTCCGTTAGAGGGAAGACGGGCCAAAAAAAAAAACCCCATGCAGGAAATAAAGtc                                                                                                                                         >  1:879545/1‑140 (MQ=255)
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTTTTATTTTTGTACCATTACGAGTCCGATAGAGGCAAGACGGCCAAAAAAAAACAAGCCGATGCAGGAAATAATGtc                                                                                                                                         >  1:2612688/1‑140 (MQ=255)
gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATTACGAGTGCGATAGAGGGAAGACCGCCAAACAAAAACAAGCCGATGCCGGAAATAATGtc                                                                                                                                         >  2:2708603/1‑140 (MQ=255)
   cTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTGTTTGTCTACGATGACGAGTGCGATAGAGGCAAGACGGCCAAACAAAAAAAAACCGATGCAGGAAATAAATTCTcc                                                                                                                                      >  1:1820609/1‑140 (MQ=255)
      tCATCCGTTTTGCTGAGACACGCGTTCACCCTCCGGCGTGCGAGACCGAGCCGTTTTTATTCTTTGTCTACGATGAGGAGTGCGATAGAGGCAAGACGTCCAATAAAAAATAAGCCGATGCAGGAAATAATGTCTCcgcg                                                                                                                                   <  1:98388/140‑1 (MQ=255)
      tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcgcg                                                                                                                                   <  2:1066628/140‑1 (MQ=255)
      tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAATCCGATGCAGGAAATAATGtatacccg                                                                                                                                   >  2:566480/1‑133 (MQ=255)
        aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTATTTGTATACCATGACGAGACCCATAGAGGCAATACGGCCAAACAAAAACAAACCCATTCAGGAAATAAATTCTCAGCGGa                                                                                                                                 >  1:3010607/1‑140 (MQ=255)
        aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATGACGAGTCCGCTAGCGGCAAGACGGCCAAACACAAACAAGCCGATGCAGGAAATAATGTCTCCGCggc                                                                                                                                 >  2:663595/1‑139 (MQ=255)
           cGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGGTTTGTTTTTGTACGATGACGAGTCCGCTAGAGGCAACACGGCCAAAAAAAAACAACCCGATGCAGGTAATAATGTGTCCGCGGACAt                                                                                                                              >  1:2435355/1‑140 (MQ=255)
             tttGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTGGTTTTGTAAGAATAAGAGTGCCCTAGAGGCAACACGGCCAAAAAAAAAAAACCCCATGCAGGAAATAAAGTAGCCCCGGAccaat                                                                                                                            >  1:2344327/1‑136 (MQ=255)
             tttGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGGTTTGTTTGTGTAAGATGACGAGTCCGATAGAGGCAACACGGCCAAACAAAAAAAACCCGATGCAGGAAATAATGTCTCCGCGGGCAttt                                                                                                                            >  1:2188513/1‑140 (MQ=255)
                ggCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTGTTGTTTGTGTAAGATGACGCGTGCGATAGAGGGAAGAAGGCCAAACACAAAAAAGCCGATGCCTGAAATAAAGTGTGCGCGGACAATTTGa                                                                                                                         >  1:2270031/1‑140 (MQ=255)
                ggCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTTTCTACGATGACGAGTCCGATATAGGCAAGACGGCCAAACAAAAAAAATCCGATGGAGGAAAAAAATTCTACGCGGACATTTTGa                                                                                                                         >  1:2965664/1‑140 (MQ=255)
                ggCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTTTCTAAGAAGGCCAGTGCGATATAGGCAAAACGGCCCAACAAAAAAAAACCCATGCAGGAAAAAAATTCTCCCCGGACAGTTTGa                                                                                                                         >  1:2838741/1‑140 (MQ=255)
                 gCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGAt                                                                                                                        <  2:2940286/140‑1 (MQ=255)
                 gCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGAt                                                                                                                        <  2:205528/140‑1 (MQ=255)
                 gCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGAt                                                                                                                        <  2:2122366/140‑1 (MQ=255)
                 gCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGAt                                                                                                                        <  2:1275821/140‑1 (MQ=255)
                     aTACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATCCAGGAAATAATGTCTCCGCGGACATTTTGATAAcc                                                                                                                    >  2:2297750/1‑140 (MQ=255)
                     aTACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACCATGACGAGTCCGAAAGAGGCACCACGGCCAAACAAAAAAAAGCCGATGCAGGAAAAAATGTCTCCGCGGACCTTTTGATAAcc                                                                                                                    >  2:2162601/1‑140 (MQ=255)
                     aTACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTAATTGTTTACGAAGACGAGACCGATATAGGCAAGACGGCCAAAACAAAACAAGCCGAATCGGGACATAATGTCTACGCGGTCATTTTGATaaac                                                                                                                    >  2:2761533/1‑138 (MQ=255)
                           gcgTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTGTTTGTCTAAGATTACGAGTCCCATAGATGGAAGACGGCCAAAAAAAAAAAATCCGATGCAAGAAATAATTTCACCGAGCAGATTTTTATAAACGaaaaa                                                                                                              >  2:3613439/1‑140 (MQ=255)
                           gcgTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTTTTCTTTGTCTACGATGACGAGTCCGGTAGAGGCAAGACCGGCCAACAAAAACAAACCCATGCAGGCAATAATGTCGCCCCGGACAGTTTTATAAACGaaaaa                                                                                                              >  1:1088073/1‑140 (MQ=255)
                           gcgTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTAATGTTTTTGTACCAAGACGAGTACCCAAGAGGCAAAAACGCCAAAAAAAAAAAAACCCATGCCGGAAAAAAATACTACCCGGACCATTTGATAAACGaaaaa                                                                                                              >  1:3565062/1‑140 (MQ=255)
                              ttCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACCAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAgcg                                                                                                           <  2:2670573/140‑1 (MQ=255)
                              ttCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAgcg                                                                                                           <  2:3010607/140‑1 (MQ=255)
                               taaCCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACTATGACTAGTCCGATAGAGTCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAgcgc                                                                                                          <  2:753198/138‑1 (MQ=255)
                               tCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAgcgc                                                                                                          <  2:1431709/140‑1 (MQ=255)
                                   cATCCGCCGTCCGAGACCGAGCCGTGGGGTTTGTTTGTGTAAGATGTCGAGTCCGGTAGATGCAAGACGGCCAACCAAAAACAAGCCGATGGGGGAAATAAATTCTCCGCGGCCATTTTGTTAACCCCAAAATCGccccg                                                                                                      >  1:941935/1‑139 (MQ=255)
                                     tCCGCCGTCCGAGACCGAGCCGTGTTTTTTGTTTTTATACGATTAAGATTGCCATATAAGCAAGACGGCCAAAAAAAAAAAAACCCATGCAGGTAATAATTTGTCCGCGGGCCATTTTATAAACCAAAAAGCGCCCCGGt                                                                                                    >  1:1752474/1‑140 (MQ=255)
                                     tCCGCCGTCCGAGACCGAGCCGTGTTTTTTCTTTGTCTACGATGACGAGTGCGATAGAGGCAAGACGGCCAAAAAAAAAAAAACCGATGGAGGAAAAAATTTCTCCCCGGGCAATTTGATAACCGAAAAAACGCCCCGGt                                                                                                    >  1:3741766/1‑140 (MQ=255)
                                     tCCGCCGTCCGAGACCGAGCCGTGTGTATTGTTTGTCGACGATGACGAGTCCGATAGAGGCAAGTCGGGCAAACAAAAACAAGCCGATGCAGGAAATAATGTGTCCGCGGCCATTTTTATAACCGAAAAAGCGCCCCGGt                                                                                                    >  1:1203151/1‑140 (MQ=255)
                                         ccgTCCGAGACCGAGCCGTTTGTATACTTTGTCTACGATGACGCGTCCGATAGAGGCAAGTCGGCCAAACAAAAACAATCCGATGCAGGAAATAATTTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGa                                                                                                <  2:2034081/140‑1 (MQ=255)
                                             ccGAGACCGAGCCGTTTGTATTCTTTTTCTACGATGACGATTCCGATAGAGGCAAGACGGCCAAAAAAAAACAAGCCGATGCAGGAAATAATTTCTACGCGGACATTTTGATAACCGAAAAAACGCCCCTGGTGGACCAg                                                                                            >  2:3900720/1‑140 (MQ=255)
                                             ccGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAATCCGATGCCGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAg                                                                                            >  2:1908434/1‑140 (MQ=255)
                                                agaCCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAAAAATGTCTCCGCGGACATTTTGATAAACGAAAAAGCGCCCCGGTTGGACAACCaa                                                                                         >  2:4025644/1‑140 (MQ=255)
                                                    cGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGcc                                                                                     <  2:3655641/140‑1 (MQ=255)
                                                      aGCCGTGTGTATTCTTTGTAGAACATGACGAGTCCGATAGAGGCAAGACGGCCCAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCCATGCCAg                                                                                   >  2:3877071/1‑140 (MQ=255)
                                                      aGCCGTGTGTATTCGTTGTCTACGATTTCGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAACCGAGGCACGAAATAAATTGGCCGCGGACAATTTGTTAACCGAAAAAGCGCCCCGGGTGGACAAGCCATGCCac                                                                                   >  2:3322678/1‑139 (MQ=255)
                                                            ttGTATTCTTGGTCTACGATGACGTGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTg                                                                             <  2:1993647/140‑1 (MQ=255)
                                                             tGTATTCTTTGTATACGATTACGAGTCCGATAGAGGCAAGAAGGCCAAACAAAAAAAAACCGATTCAGGAAAAAAATTATACGCCGACAATTTGAAAAACGAAAAAACGCCCCTGTTTGACAACCAAATCCAGCTATTGt                                                                            >  2:1509361/1‑140 (MQ=255)
                                                              gTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGtt                                                                           <  2:2458080/140‑1 (MQ=255)
                                                                  tCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGcc                                                                       <  1:3411427/140‑1 (MQ=255)
                                                                  tCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGcc                                                                       <  1:207744/140‑1 (MQ=255)
                                                                  tCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGcc                                                                       <  1:2833137/140‑1 (MQ=255)
                                                                   cTTTGTATACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCa                                                                      <  1:3435705/140‑1 (MQ=255)
                                                                             gATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCa                                                           <  1:2331043/140‑1 (MQ=255)
                                                                              aTGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCa                                                           <  1:3634335/140‑1 (MQ=255)
                                                                                  cGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCg                                                       >  2:3109677/1‑140 (MQ=255)
                                                                                  cGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCg                                                       >  2:3478310/1‑140 (MQ=255)
                                                                                      tCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCtt                                                   >  2:3371015/1‑140 (MQ=255)
                                                                                      tCCGATAGAGGCAAGACGGCCAAACAAAAAAAAGCCGATGCAGTAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCtt                                                   <  1:2038629/140‑1 (MQ=255)
                                                                                           tAGAGGCAAGACGGCCAAACAAAAACAATCCGATGCAGGAAATAAAGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATACTCAGGCCACCCGGCTTGCCTg                                              >  2:3790079/1‑140 (MQ=255)
                                                                                            agagGCAAGACGGCCAAACAAAAACCAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGc                                             <  2:3413900/140‑1 (MQ=255)
                                                                                            agagGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCGCCGCGGACATTTTGATAACCGAAAACGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGc                                             <  2:1088765/140‑1 (MQ=255)
                                                                                              agGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCaa                                           <  2:1158840/140‑1 (MQ=255)
                                                                                                gCAAGACTGCCAAAAAAAAACAAGCCGATTCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGATCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg                                         >  1:1007321/1‑140 (MQ=255)
                                                                                                gCAAGACGGCCAAACAAAAACAAGCCTATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCGCTTGTTCGCCATATTCAGGCCACCCGGCTTGCCTGCAAAg                                         >  1:1007757/1‑140 (MQ=255)
                                                                                                gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg                                         <  1:3381836/140‑1 (MQ=255)
                                                                                                gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg                                         <  2:444242/140‑1 (MQ=255)
                                                                                                gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg                                         >  2:1572324/1‑140 (MQ=255)
                                                                                                gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg                                         >  1:3218905/1‑140 (MQ=255)
                                                                                                 cAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGc                                        <  2:3565062/140‑1 (MQ=255)
                                                                                                 cAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCGCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGc                                        <  1:1123412/140‑1 (MQ=255)
                                                                                                 cAAGACGGCAAAAAAAAAAAAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGc                                        <  2:3783753/140‑1 (MQ=255)
                                                                                                      cGGCCAAACAAAAACAAGCCTATGCATGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  1:497725/140‑1 (MQ=255)
                                                                                                      cGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  2:879545/140‑1 (MQ=255)
                                                                                                      cGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  1:2772595/140‑1 (MQ=255)
                                                                                                      cGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  1:3096447/140‑1 (MQ=255)
                                                                                                      cGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  1:867570/140‑1 (MQ=255)
                                                                                                      cGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  2:2612688/140‑1 (MQ=255)
                                                                                                      cGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGAGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  2:1326284/140‑1 (MQ=255)
                                                                                                      cGGCCAAAAAAAAACAAGCCGATGCAGGAAATTATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  2:287487/140‑1 (MQ=255)
                                                                                                      cGGCAAAACAAAAACAAGCAGATTCAGGAAATAATGTCTCCGCGGACATTTTGATAACCTAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCaa                                   <  1:3787441/140‑1 (MQ=255)
                                                                                                         ccAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCACTAg                                >  1:691952/1‑140 (MQ=255)
                                                                                                           aaacaaaaacaaGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTc                              <  1:1572324/140‑1 (MQ=255)
                                                                                                             acaaaaacaaGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCg                            <  1:936893/140‑1 (MQ=255)
                                                                                                                       gCCGATGCAGGACATAAGGTCTCCGCGGACATGGTGATAACCGACAAAGCGCCCCGGTTGGACAAGGAATGCCAGCTCTTGTTCGCCATAAGCAGGCCACCCGGCTTGCCTGCAAAGCCGCACTCGGCCGGGGGGATGtt                  >  2:1832254/1‑140 (MQ=255)
                                                                                                                        ccGATGCAGGAAATAATGTCTCCGCGTACATTTTGATAACCGATACAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGttc                 <  1:3473985/140‑1 (MQ=255)
                                                                                                                        ccGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGttc                 <  1:123996/140‑1 (MQ=255)
                                                                                                                        ccGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGGTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGttc                 <  1:425402/140‑1 (MQ=255)
                                                                                                                           aTGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCa                                    >  1:3280471/1‑118 (MQ=255)
                                                                                                                                 gAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAg        <  2:1294559/140‑1 (MQ=255)
                                                                                                                                 gAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAg        <  2:764187/140‑1 (MQ=255)
                                                                                                                                 gAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAg        <  2:839717/140‑1 (MQ=255)
                                                                                                                                      aatGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCGTGTTGGACAAGCAATGCCAGCTCTTGTGCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCaa   >  1:2019755/1‑140 (MQ=255)
                                                                                                                                      aatGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCACAGCCGCAATAGTCCGGGGGTCTGGTCTTCATTAAGCTCaa   >  1:2508586/1‑140 (MQ=255)
                                                                                                                                      aatGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCACTAGTCCGGGGGTATGTTCTTCATTAAGCTCaa   >  1:1177150/1‑140 (MQ=255)
                                                                                                                                      aatGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCaa   <  2:1232732/140‑1 (MQ=255)
                                                                                                                                      aatGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCaa   >  2:327406/1‑140 (MQ=255)
                                                                                                                                      aatGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCaa   >  1:1507955/1‑140 (MQ=255)
                                                                                                                                      aatGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCaa   <  2:1735865/140‑1 (MQ=255)
                                                                                                                                       atGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCAAt  <  1:804328/140‑1 (MQ=255)
                                                                                                                                       atGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCAAt  <  2:1599156/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGCAATAGTCCGGGGGTATGTTCTTCATTAAGCTCAAT  >  NZ_CP034943/1151907‑1152181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: