Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,366,028 A→C 9.9% T176P (ACG→CCG)  EO946_RS07135 → PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,366,0280AC 9.9% 416.7 / 3.6 176T176P (ACG→CCG) EO946_RS07135PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (65/84);  new base C (3/23);  total (68/108)
Fisher's exact test for biased strand distribution p-value = 1.86e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GAAGATGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTG  >  NZ_CP034943/1365893‑1366163
                                                                                                                                       |                                                                                                                                        
taagaTGGATAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAcggg                                                                                                                                      <  2:2550649/139‑1 (MQ=255)
gaagaTGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAcggg                                                                                                                                      >  1:488919/1‑140 (MQ=255)
gaagaTGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGAAGTAGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAAAACCGATTACTAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAcggg                                                                                                                                      >  1:3960301/1‑140 (MQ=255)
caagaTGGAGAAACCTACCAAGTGAATATATACATTATTCCAAACTCACTCTTGTCTGCTGATCGAATCAGCGGTTAAGATCAACACAACACACCGATTAGGAAAAAACAAGCCAAAACCAGTGCTGATCGATAATcggg                                                                                                                                      <  2:488324/139‑1 (MQ=255)
       gagaAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAAcggg                                                                                                                                      <  1:709095/133‑1 (MQ=255)
       gagaAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACa                                                                                                                              <  2:615059/140‑1 (MQ=255)
       gagaAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACa                                                                                                                              <  1:1696778/140‑1 (MQ=255)
       gagaAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACa                                                                                                                              <  2:1838548/140‑1 (MQ=255)
               tACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGa                                                                                                                      >  2:70525/1‑140 (MQ=255)
                 cGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAACACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACg                                                                                                                    <  2:1313521/140‑1 (MQ=255)
                    aGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAg                                                                                                                 >  1:3455903/1‑140 (MQ=255)
                      tGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAACACCAGTGCTGAGCGAGAACCGGGCGGGACAGCGGATGACGCAGag                                                                                                               >  1:2961336/1‑139 (MQ=255)
                      tGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGag                                                                                                               >  1:793583/1‑139 (MQ=255)
                        aaGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGAtct                                                                                                             >  2:1168462/1‑140 (MQ=255)
                        aaGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGAtct                                                                                                             >  2:2316066/1‑140 (MQ=255)
                        aaGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGAtat                                                                                                             >  2:2141353/1‑138 (MQ=255)
                               aCATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGaaaaa                                                                                                      >  2:4003614/1‑140 (MQ=255)
                                  tGAATGCAAAAGCAGTCGTGTCTGCTGCTCGAATCAGCGGTGAAGATCAACAAAAAACACCGAGTACGAAAAAAGAAGCCAAATCCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGt                                                                                                   >  1:262966/1‑140 (MQ=255)
                                  tGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGt                                                                                                   >  2:1464905/1‑140 (MQ=255)
                                  tGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGt                                                                                                   <  2:3107945/140‑1 (MQ=255)
                                  tGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGt                                                                                                   >  2:3780872/1‑140 (MQ=255)
                                  tGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGt                                                                                                   <  2:733095/140‑1 (MQ=255)
                                  agAATGCAAAAGCAGTCGTGTATGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGt                                                                                                   <  2:2815155/139‑1 (MQ=255)
                                   gAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTg                                                                                                  <  1:197900/140‑1 (MQ=255)
                                   gAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTg                                                                                                  <  1:1439175/140‑1 (MQ=255)
                                    aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACATCGGATGACGCAGATCTCGAAAAAAGTGa                                                                                                 >  1:1856420/1‑140 (MQ=255)
                                    aaTGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGa                                                                                                 >  2:3809155/1‑140 (MQ=255)
                                       gCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAggg                                                                                              <  2:1271178/140‑1 (MQ=255)
                                       gCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAggg                                                                                              <  2:1419232/140‑1 (MQ=255)
                                             gCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCActct                                                                                        <  1:2075274/140‑1 (MQ=255)
                                             gCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCActct                                                                                        <  1:3876897/140‑1 (MQ=255)
                                              cAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACtctc                                                                                       >  2:3869777/1‑140 (MQ=255)
                                                gTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCAt                                                                                     >  1:3165359/1‑140 (MQ=255)
                                                gTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCAt                                                                                     >  1:170123/1‑140 (MQ=255)
                                                  cGTGTCTGCTGATCGAATCAGCGCTGAAGATCAACAAAAAACACCGATTACGAACAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCt                                                                                   <  2:1937359/140‑1 (MQ=255)
                                                   gtgtCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCtt                                                                                  <  2:3881268/140‑1 (MQ=255)
                                                     gtCTGCTTATCGAATAATCGTTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGTGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTg                                                                                <  2:3960301/140‑1 (MQ=255)
                                                      tCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGa                                                                               >  1:3325271/1‑140 (MQ=255)
                                                      tCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGa                                                                               >  1:886622/1‑140 (MQ=255)
                                                      tCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGa                                                                               >  1:3348042/1‑140 (MQ=255)
                                                       ctgctgATCGAATCAGCGGTGAAGATCAACAAACAACACCGAGTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGaa                                                                              <  2:1921503/140‑1 (MQ=255)
                                                       ctgctgATCGAATCAGCGGTGAAGATCAACAAAAACCACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGaa                                                                              <  1:3682409/140‑1 (MQ=255)
                                                       ctgctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGaa                                                                              <  1:1510139/140‑1 (MQ=255)
                                                       ctgctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGaa                                                                              <  2:1250900/140‑1 (MQ=255)
                                                       ctgctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGaa                                                                              <  2:556003/140‑1 (MQ=255)
                                                         gctgATCGAATCAGCGGTGAAGATCAACAAACAACCCCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  1:2362168/140‑1 (MQ=255)
                                                         gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  2:3998955/140‑1 (MQ=255)
                                                         gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  2:1489572/140‑1 (MQ=255)
                                                         gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  2:2615938/140‑1 (MQ=255)
                                                         gctgATCGAATCAGCGGTGAAGATCAACAAAAAACACAGATTACGAAAAAAGAACCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGt                                                                            <  1:2521698/140‑1 (MQ=255)
                                                           tgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTgg                                                                          >  2:137561/1‑140 (MQ=255)
                                                           tgATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCAGTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATGTTTGAGGTgg                                                                          >  2:1523647/1‑140 (MQ=255)
                                                            gATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGa                                                                         <  2:886622/140‑1 (MQ=255)
                                                            gATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGa                                                                         <  2:3444995/140‑1 (MQ=255)
                                                             aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  2:1082383/1‑140 (MQ=255)
                                                             aTCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGAt                                                                        >  1:1474917/1‑140 (MQ=255)
                                                               cGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCCTTGCTGAGCGAGAAACGGGCGGGACAGCGGAGGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTGGGAAGTGGAGGt                                                                      >  1:3490707/1‑140 (MQ=255)
                                                               cGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCt                                                                                   >  1:1766543/1‑127 (MQ=255)
                                                               cGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCt                                                                                   <  2:1766543/127‑1 (MQ=255)
                                                               cGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCt                                                                                   >  1:3828151/1‑127 (MQ=255)
                                                               cGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGt                                                                      >  1:2664666/1‑140 (MQ=255)
                                                               cGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGt                                                                      >  1:524883/1‑140 (MQ=255)
                                                                gAATCAGCGGTGAAGATCAACAAAAAACACCGCTTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGtt                                                                     <  1:2631612/140‑1 (MQ=255)
                                                                  aTCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGa                                                                   >  2:2645185/1‑140 (MQ=255)
                                                                           gAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  1:1195204/140‑1 (MQ=255)
                                                                           gAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          >  1:197451/1‑140 (MQ=255)
                                                                           gAAGATCAACAAAAAACACCGATTACGAAAAAACAAGCCAAAACCATTGCTGAGCGAGAAACCGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGga                                                          <  2:29285/140‑1 (MQ=255)
                                                                            aaGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGa                                                                                                                         <  1:3545057/76‑1 (MQ=255)
                                                                            aaGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGGTGATCTTCCGgat                                                         >  1:2773854/1‑140 (MQ=255)
                                                                            aaGAGCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATATTCCGgat                                                         >  2:2993466/1‑140 (MQ=255)
                                                                             aGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGgatg                                                        <  1:2867771/140‑1 (MQ=255)
                                                                                         aaCACCGTTTACCAAAAAACACGACAAAACCAGTGCGGAGCGATCCCGGGGGGGGACCGGGGATGACGCAGTTCTCCAAAAATGTGAGGGCACTCTCATCTTTGATGTGTTTTTTGATGTTCCGGATTATAAACACGCGa                                            <  2:524883/140‑1 (MQ=255)
                                                                                                            taacccAAAACCTTTGCTGAGCGACAACGGGGGGGGACAGGGTATGACTCATATCTCTAAAAAAGTGTGGGCACTCTAATTTTTTATGTGTTTTTTGATCTTCCGTATTATAAAGACGCGACTGTGAAAATTAATGCCTa                         <  1:1230430/136‑1 (MQ=255)
                                                                                                                    aaCCTTTGCTGCGCGATAACCGGGCGGGACTGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCTTCTTTGATGTGTTTGTTGATCTTCCGGATGATAAAGACGCGACTGTGAAAATTAATGCCTACACCGGaa                 <  2:2867455/140‑1 (MQ=255)
                                                                                                                    aaCCATTGCTGAGCTATAACGGGGCGTGACAGCGTATAACCAAGATCTCCAAAAAATTTAGGGCACTATCATCTTTGAAGTGTATGTTTATCTTCGGTATCATAACCAAGCGACTGTGAAAATTAATGCCTACACCGGaa                 <  2:2708402/140‑1 (MQ=255)
                                                                                                                    aaCCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGaa                 >  1:2437219/1‑140 (MQ=255)
                                                                                                                        tttGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCTAAAAATGTGAGGGAAATATCATCTTTTAAGTGTATGTTGATCTTCCGGATGATAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGt             <  2:3014400/139‑1 (MQ=255)
                                                                                                                        tttGCTGAGCGACAACGGGGGGGGACAGCGGATGACGCAGATCTCTAAAAAATTGAGGGAACTCTCATGTTTGAAGTTGTTGTTTATCTTCGGGATTATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGt             <  1:76859/139‑1 (MQ=255)
                                                                                                                          tGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTg           >  2:3829692/1‑140 (MQ=255)
                                                                                                                          tGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACAACGGAAAGGTTg           >  2:3748903/1‑140 (MQ=255)
                                                                                                                           gCTGAGCCATAAACGGGGGGGACAGCGTATGACGCAGATCTCGAAAAAATTGAGGGCACTCTCATCTTTTAAGTGGATGTTTATCTTCCGGATTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGc          <  2:907384/140‑1 (MQ=255)
                                                                                                                            ctgctcGACAACCGGGCGGTACCCCGGATGACCCATATCTCCAAAAAAGTGATGCCACTCTCATCTTTGACGTGTATGTTGATCTTCCGTATTATAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  2:2419996/135‑1 (MQ=255)
                                                                                                                            ctctgCGAGACACGGGCGGGACCGGGGTTGCCGCCTCTCTCGAAAAATGTGAGGCCACTATCATGTTTGATGTGTTTGTTGATCTTGCGTATGATACAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  2:3422089/136‑1 (MQ=255)
                                                                                                                            cTGAGCGAGAAAGGGGCGGGACAGCGTATGACGAATATCTCGAAAAAAGTGAGGGAACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  2:3348042/140‑1 (MQ=255)
                                                                                                                            cTGAGCGACACACGGGCGGGACAGCGTATGACCCAGATCTCGAAAAAAGTGCGGCCACTCTAATCTTTGAAGTGGTTGTTGATCTGCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  2:318209/140‑1 (MQ=255)
                                                                                                                               aGCGAGAAACGGGCGGGACAGCGTATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCa         <  2:748838/137‑1 (MQ=255)
                                                                                                                               aGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAAc      >  1:2287589/1‑140 (MQ=255)
                                                                                                                               aGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAAc      >  1:733676/1‑140 (MQ=255)
                                                                                                                                gctataaaCGGGCGGTACCGCGTAGCACGCATTTCTACAAAAAAGTTTGGGCACTTTATTTTTTTATGTGTTTTTTTATATTCGGGATGATAAACAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  2:685901/135‑1 (MQ=255)
                                                                                                                                gctacaaaCGGGCGGCACAGCGTATGACGCATATCGCTAAAAATGTGAGGGCACTATAATCTTTCATGTGTTTTTTTATCTTCCGTTTTATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  1:1169870/135‑1 (MQ=255)
                                                                                                                                gCGAGACAGGGGGGGGACAGGGGATCACGCAGATCTCTAAAAATGTGAGGCCACTCTAATTTTTGATGTGGTTTTTTATCTTCCGTTTCATAACCCCGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  1:2424357/140‑1 (MQ=255)
                                                                                                                                gCGACAACCGGGCGGGACAGCGGTTGACGAACATCTCTAAAAAAGTGAGGCCACTCTAATCTTTGAAGTGGATGTTGATCTTCCGTATCATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  2:3548506/140‑1 (MQ=255)
                                                                                                                                cctagaAACGGCGGCGACAGCGCAGCACCCAGAACGCTAAAAAAGAGAGGCAACTCTCATTTTTCATGTCGATGTTTATGTCCCGTATCAAAAATAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  1:573382/137‑1 (MQ=255)
                                                                                                                                ccGATAAAGGGGCGGCACCGCGGATGCCGCATATCCCGAAAAATTTGAGGGCAATCTCATCTTTGATGTTGTTGTTTATGTTCCGGTGGATAACCCCGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  1:978830/139‑1 (MQ=255)
                                                                                                                                ccGACAACCGGGCGGAACAGGGTATCACGAACAGCTCTAAAAAAGTCAGCCCCACCGAATCTTGCACGGGGAGGTCGAACTCCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  1:1308852/139‑1 (MQ=255)
                                                                                                                                 gcgacaaCGGGCGGGTAAAGGGGTTGACGAAGATCTAAAAAAAAGTGAGCCCACTATAATCTTTGAAGTGGTTTTTCATCTGCCGTATCATAAACACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACa     <  1:2993466/135‑1 (MQ=255)
                                                                                                                                 cGAGAAACGGGCGCCACCGCGGCTGACCCATATCTCTAAAAACGTGAGCGCCCAAAAATCTTTGACGTGGATGTTGAAAAACCGGATCATAACGAAGCGACTGTGAAAATTAATCCCTACACCGGAAAGGTTGCAAACAt    <  2:849983/140‑1 (MQ=255)
                                                                                                                                   cgaACCGGGGGGGACCCCGGATGCGGCACAGCTCTAAAAAAGGGCGCGCACTCACATCTTTTAAGTGGATGTTGATGTCCCGGTTTATAAAGACGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATtg  <  2:3567026/139‑1 (MQ=255)
                                                                                                                                   agaACCGGGGGCCACAGCTGATGACCCAAAACTCCAAAAAAGTTAGGGCACTATAATCTTGGTAGTGGTTTTTTATATTCCGTATTATAAACCCGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATtg  <  1:126450/140‑1 (MQ=255)
                                                                                                                                   agaAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATtg  >  1:685901/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GAAGATGGAGAAACATACGAAGTGAAGATAGACATGAATGCAAAAGCAGTCGTGTCTGCTGATCGAATCAGCGGTGAAGATCAACAAAAAACACCGATTACGAAAAAAGAAGCCAAAACCATTGCTGAGCGAGAAACGGGCGGGACAGCGGATGACGCAGATCTCGAAAAAAGTGAGGGCACTCTCATCTTTGAAGTGGATGTTGATCTTCCGGATGATAAAGAAGCGACTGTGAAAATTAATGCCTACACCGGAAAGGTTGCAAACATTG  >  NZ_CP034943/1365893‑1366163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: