BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,174	T→G	8.3%	N120H (AAC→CAC)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,174	0	T	G	8.3%	719.8 / 14.0	302	N120H (AAC→CAC)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (150/117); new base G (32/3); total (182/120)
Fisher's exact test for biased strand distribution p-value = 2.93e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

TCACGCGTGACAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTG  >  NZ_CP034943/240036‑240311

tCACGCGTGACAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGttt                                                                                                                                          <  2:3340095/140‑1 (MQ=255)

tCACGCGTGACAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTGTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGttt                                                                                                                                          >  1:1960385/1‑140 (MQ=255)

             tGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGAtttttttt                                                                                                                             >  1:2477539/1‑140 (MQ=255)

             tGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTtttgtt                                                                                                                             >  1:264393/1‑140 (MQ=255)

              gCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTtttgttt                                                                                                                            <  1:2152773/140‑1 (MQ=255)

               cGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTGTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTtttgtttg                                                                                                                           >  2:243379/1‑140 (MQ=255)

                  cTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGaat                                                                                                                        >  2:2101979/1‑139 (MQ=255)

                     tggtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGttt                                                                                                                     >  2:838645/1‑140 (MQ=255)

                       gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTg                                                                                                                   >  1:905909/1‑140 (MQ=255)

                       gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTg                                                                                                                   >  1:79121/1‑140 (MQ=255)

                        tggAGAAGGTGTAAGAGAGAGAGAAGGCGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTgg                                                                                                                  <  1:3061046/140‑1 (MQ=255)

                        tggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTgg                                                                                                                  <  2:589147/140‑1 (MQ=255)

                        tggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTgg                                                                                                                  <  2:2197394/140‑1 (MQ=255)

                          gagaGGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTTAAGTTTTGGga                                                                                                                >  2:1133376/1‑140 (MQ=255)

                          gagaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTgggc                                                                                                                >  2:3471603/1‑139 (MQ=255)

                          gagaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGga                                                                                                                <  2:2992633/140‑1 (MQ=255)

                            gaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGCTTTTTGTTTGAAGTTTTGGgaga                                                                                                              >  1:3041272/1‑140 (MQ=255)

                            gaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGgaga                                                                                                              >  1:1771645/1‑140 (MQ=255)

                               ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGAttt                                                                                                           >  1:295662/1‑140 (MQ=255)

                               ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAttt                                                                                                           >  2:2607211/1‑140 (MQ=255)

                                 tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGa                                                                                                         >  2:908317/1‑140 (MQ=255)

                                  gttagagagagagAATGAGGATCTCTGATGTTGTTTTTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATAATTCCGAGTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                        <  2:1899288/137‑1 (MQ=255)

                                    aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAtttttatt                                                                                                      >  1:855713/1‑135 (MQ=255)

                                     agagagagagAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAAttg                                                                                                     >  1:105114/1‑138 (MQ=255)

                                         agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTATGTTTTTAGTTTTGGGAGATTTGAATGGCttt                                                                                                 >  1:1988791/1‑140 (MQ=255)

                                          gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCtttt                                                                                                >  2:2754288/1‑140 (MQ=255)

                                          gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATTGAtttt                                                                                                >  1:3756623/1‑140 (MQ=255)

                                           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATGGCttttg                                                                                               >  1:2570262/1‑139 (MQ=255)

                                           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCCGATTTTTGTTTGAAGTTTTGGGAGCTTTGAATGGCTTTTc                                                                                               >  2:2459876/1‑140 (MQ=255)

                                           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                               >  2:649740/1‑140 (MQ=255)

                                           agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTGCGGAATTTTGTTTGACGTTTTGTGAGATTTGAAAGGCATTTc                                                                                               >  2:1317293/1‑140 (MQ=255)

                                               aaGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTATTTTTTGGAGATTTGAATTGCTTTTGCGtt                                                                                           >  1:3499793/1‑140 (MQ=255)

                                                 ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAATTTTTGGGAGATTTGAATGGCTTTTCCGtttt                                                                                         >  2:3836400/1‑140 (MQ=255)

                                                 ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAATGGCTTTTACGtttt                                                                                         >  2:1353463/1‑140 (MQ=255)

                                                 ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTGTTCCGtttt                                                                                         >  2:4001529/1‑140 (MQ=255)

                                                  gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGttttt                                                                                        <  1:1311084/140‑1 (MQ=255)

                                                  gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTGGAATGGCTTTTCCGttttt                                                                                        <  1:1170433/140‑1 (MQ=255)

                                                    gCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTaa                                                                                      <  1:471225/140‑1 (MQ=255)

                                                    gCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTaa                                                                                      <  1:2196506/140‑1 (MQ=255)

                                                     cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTaaa                                                                                     >  2:545379/1‑140 (MQ=255)

                                                       tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAGTTGAATGGCGTTTCAGTTTTTaaaaa                                                                                   >  2:3589313/1‑139 (MQ=255)

                                                        ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAACTTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAACa                                                                                  >  2:1663554/1‑140 (MQ=255)

                                                        ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                  <  1:3728981/140‑1 (MQ=255)

                                                        ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                  <  1:2588868/140‑1 (MQ=255)

                                                          ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAag                                                                                >  2:1559172/1‑140 (MQ=255)

                                                            gaggatGGAATTATTCAGAGATGCGGTTTTTGTATTGTTGTGCTAATACGAAGTCGATCATTCCGACTCTACGGGGGTTTTCGGAGTTTTGTTGGGAGTTGTGGGAGCGTTGAATGGCTGTTGCGGTGTTAAAACCagag                                                                              >  2:3207813/6‑140 (MQ=255)

                                                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTTAAACAAGAGGCAAGc                                                                        >  1:1879424/1‑140 (MQ=255)

                                                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCACGc                                                                        >  2:1088097/1‑140 (MQ=255)

                                                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGGTTTTAAAAAAAGAGGAAAGc                                                                        >  1:708454/1‑140 (MQ=255)

                                                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAACAAGAGGCACGc                                                                        >  2:687019/1‑140 (MQ=255)

                                                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                        >  2:2021449/1‑140 (MQ=255)

                                                                  ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAATGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                        >  1:3541357/1‑140 (MQ=255)

                                                                  gcaaTTATTCAGAGATGCTGATTTTGTATTTTTGATCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGCAGATTTTAATTGCTTTTCCGTGTTTAAAACAAAAGGCAAGc                                                                        >  2:389470/3‑140 (MQ=255)

                                                                   gAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGTGATTTTAATTGCTTTTCCGTTTTTAAAAAAAGATGCAAAcc                                                                       >  1:677392/1‑140 (MQ=255)

                                                                        attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTTAAGTTTTTGGAGATTTGTATTGGTTTTGCGTTTTTTAAAAAAGAGGCAAGCCACATa                                                                  >  1:1019036/1‑140 (MQ=255)

                                                                        attCAGAGATGCTGTTTTTGTATTTTTGTTCTAAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                  <  2:1734655/140‑1 (MQ=255)

                                                                         ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCTATCATTCCGACTATAAGGGCGTTTTCGGAATTTTGTTTGAAGGTTTGGTAGATTTGAATGGCTTTTACGTTTTTAAAACACAATCCAATCCAGATaa                                                                 >  1:342203/1‑140 (MQ=255)

                                                                              agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCg                                                            >  2:1254907/1‑140 (MQ=255)

                                                                                  gCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaa                                                        <  2:3842207/140‑1 (MQ=255)

                                                                                  gCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaa                                                        <  2:3544011/140‑1 (MQ=255)

                                                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTTGAGATTTGAATTGGGTTTAAGTTTTTAAAAAAAAAGTCAAGACAGAAAAAACCGacaca                                                       >  1:1928157/1‑138 (MQ=255)

                                                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                       >  2:2033992/1‑140 (MQ=255)

                                                                                   cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGCTTTTTGTTTGTAGTTTTGGGAGATTTTTATTGCTTTTCCGTTTTTTAAACAATAGGCAAGCCAGATAAACCCCgcaac                                                       >  1:151824/1‑140 (MQ=255)

                                                                                     gTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaa                                                     <  1:3969056/140‑1 (MQ=255)

                                                                                      tttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaac                                                    >  2:1761728/1‑140 (MQ=255)

                                                                                       ttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaaca                                                   <  1:596574/140‑1 (MQ=255)

                                                                                        tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAATTTTGGGAGATTTTAATTGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGAcaacaacaa                                                  >  1:2045777/1‑140 (MQ=255)

                                                                                        tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGAcaacaacaa                                                  >  2:3917358/1‑140 (MQ=255)

                                                                                         ttGTATTTTTGTTCTAATTCGAAGTCGATCAGTCAGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTGGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACaaa                                                 <  1:2863082/140‑1 (MQ=255)

                                                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAATCCAGATAAAACCGACAACAACAAAAaataaa                                          >  2:3150131/1‑139 (MQ=255)

                                                                                                tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGGTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCTACAAAAAACAAAaataat                                          >  2:3766114/1‑140 (MQ=255)

                                                                                                    ttCTAATCCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGCAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                      <  2:2670846/140‑1 (MQ=255)

                                                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                      <  2:1939942/140‑1 (MQ=255)

                                                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                      <  1:1663554/140‑1 (MQ=255)

                                                                                                    ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                      >  2:737181/1‑140 (MQ=255)

                                                                                                      cTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCa                                    >  2:440387/1‑140 (MQ=255)

                                                                                                         ctccGAAGTCGCACCTTCCGACTATACGGGCGTTTTAGGATTTTAGTATGAAGTTTTGGGAGAGTTGACTGGCTTTTCCGTTTTTAAACCAAGAGGCAAGCCAGATAAACCCGACACCAACAAAAAATAATCTAGCAAGt                                 <  1:1732457/137‑1 (MQ=255)

                                                                                                         aTACGAAGTCGATCATTCCGCCTATACGGGCGTTTTCGGATTTTTGTTTGCAGTATTGGGATAGTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                 <  1:3178318/140‑1 (MQ=255)

                                                                                                              aaGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTTTAGTTTTTGGTGTTTTGTATTTGTTTTGCGTTTTTTAAAAAAGATGGAAACCACAAACACACGAGCACAAAAAAAAAAAAAATAGCAAATaaaaa                            >  1:1379136/1‑138 (MQ=255)

                                                                                                                gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                          <  2:3425061/140‑1 (MQ=255)

                                                                                                                gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                          <  2:873611/140‑1 (MQ=255)

                                                                                                                gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                          <  2:1509314/140‑1 (MQ=255)

                                                                                                                gTCGAACAGTCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGAAGTGTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                          <  1:3333052/140‑1 (MQ=255)

                                                                                                                   gATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAAAAATCTAGCAACTAAACTAcatg                       >  2:2344063/1‑140 (MQ=255)

                                                                                                                     tCATTCCGACTATACGGGCGTTTTCTGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                     <  1:2344063/140‑1 (MQ=255)

                                                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                     <  2:2569563/140‑1 (MQ=255)

                                                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                     <  1:3144245/140‑1 (MQ=255)

                                                                                                                     tCATTCCGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGCGAGAGTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                     <  1:190108/140‑1 (MQ=255)

                                                                                                                     tCATTCCGACTAGACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGGGAGATTTGTATGGCTTTTCCGTTTTTAAAACAAGAGCCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                     <  1:3684041/140‑1 (MQ=255)

                                                                                                                           cGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAAATCCAGGCATGGGGt               >  2:3061803/1‑140 (MQ=255)

                                                                                                                             aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCAAGCATGGGGTaa             >  2:127577/1‑140 (MQ=255)

                                                                                                                                  aCGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGGTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAAATCCCATCATGGGGTaaataat        >  1:3859810/1‑135 (MQ=255)

                                                                                                                                   cGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAACCCCACAACAAAAAAAAATAATCTAGCAAGGAAACTCCATGCATGGGGGAattcctt       >  2:3488075/1‑140 (MQ=255)

                                                                                                                                   cGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattcatt       >  2:1003395/1‑140 (MQ=255)

                                                                                                                                      gCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAAACCGACAAAAAAAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAAATCATTCGt    >  2:2227857/1‑140 (MQ=255)

                                                                                                                                      gCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGt    <  1:2550577/140‑1 (MQ=255)

                                                                                                                                      gCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGt    <  1:2715463/140‑1 (MQ=255)

                                                                                                                                      gCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACACGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGt    <  1:973719/140‑1 (MQ=255)

                                                                                                                                       cGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGAATTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGtg   >  2:371667/1‑139 (MQ=255)

                                                                                                                                        gTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACCACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTg  >  2:1801718/1‑140 (MQ=255)

TCACGCGTGACAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTG  >  NZ_CP034943/240036‑240311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`