Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,805,602 G→C 6.5% V794V (GTG→GTC EO946_RS09140 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,805,6020GC 6.5% 684.5 / 31.5 212V794V (GTG→GTCEO946_RS09140non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (105/92);  new base C (6/8);  total (111/101)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 215 total aligned reads displayed.

TTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTTATAAATCGGGTGAT  >  NZ_CP034943/1805464‑1805740
                                                                                                                                          |                                                                                                                                          
  aCGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCg                                                                                                                                         <  2:1152252/140‑1 (MQ=255)
        cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                                                                   <  2:1903887/140‑1 (MQ=255)
        cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                                                                   <  1:213223/140‑1 (MQ=255)
        cTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGAAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGAGGACCAGCATTAGAAACATGTGTCGATCGGa                                                                                                                                   >  1:2785867/1‑140 (MQ=255)
        cTACTGAAGCCTCCATTTACGCTGCGTAGTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATCTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGa                                                                                                                                   >  1:2103542/1‑140 (MQ=255)
         aaCTTAAGCCTCCATTTAATATTATTATTTCGGATGATGAAAAGGAGATATTGCAAGTCACCATACGCCGATTTGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaa                                                                                                                                  >  2:2644994/2‑140 (MQ=255)
          acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                                                                 >  1:646326/4‑140 (MQ=255)
          acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                                                                 >  1:2930419/4‑140 (MQ=255)
          acggAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATCGCAAGTCACCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGaaa                                                                                                                                 >  1:3491994/4‑140 (MQ=255)
          aCTGAAGCCTCCATTTACGCTGCTTATTTCGGATGCGGAAAAGGAGATAATGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                                                                 <  2:2487941/140‑1 (MQ=255)
          aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaca                                                                                                                                 <  2:1974024/140‑3 (MQ=255)
          aCTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGaaa                                                                                                                                 >  2:3224222/1‑140 (MQ=255)
                gCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTgg                                                                                                                           >  1:1334070/1‑140 (MQ=255)
                 gcTCCATTTACTCTGCGTATTTCTGTTGCTGAAAAGGATATATTGGAAGTCACAATACGCCGTTTGGAAAGGCTGTCTCCAACATGCATATTTATATTGTGGACCAGCATTTGAAACCTGTGTCTATCGGAAAGGCTGGt                                                                                                                          <  2:3362805/139‑1 (MQ=255)
                 ccTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGt                                                                                                                          <  1:3470749/140‑1 (MQ=255)
                 ccTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCAACATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTTTCGATCGGAAAGCCTGGt                                                                                                                          <  2:3618669/140‑1 (MQ=255)
                  cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTg                                                                                                                         >  2:1362836/1‑140 (MQ=255)
                  cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                                                         >  2:1646900/1‑140 (MQ=255)
                  cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                                                         >  1:800151/1‑140 (MQ=255)
                  cTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTg                                                                                                                         >  1:249629/1‑140 (MQ=255)
                   tCCATTTACGCTGCGTATTTCGGATGCGGAACAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGACAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGa                                                                                                                        >  2:2186944/1‑140 (MQ=255)
                   tCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGa                                                                                                                        >  1:3905327/1‑140 (MQ=255)
                    ccATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAg                                                                                                                       <  2:72615/140‑1 (MQ=255)
                    ccATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAg                                                                                                                       <  1:1646900/140‑1 (MQ=255)
                     cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                                                                      >  1:3195766/1‑140 (MQ=255)
                     cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                                                                      >  1:3334961/1‑140 (MQ=255)
                     cATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGc                                                                                                                      >  1:3632560/1‑140 (MQ=255)
                         tACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTg                                                                                                                  <  1:223658/140‑1 (MQ=255)
                               gCGTATTTCGGATGCGGAAAAGGATATATTGCAAGTCACCATACGCCGATTGGAAAGACTGTCTCCAACATGAAAATTTATATTGTGGACAAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGAATctc                                                                                                            >  2:1056874/1‑138 (MQ=255)
                               gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                                                            >  1:4003344/1‑140 (MQ=255)
                               gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                                                            >  1:243477/1‑140 (MQ=255)
                               gCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGc                                                                                                            >  1:2209097/1‑140 (MQ=255)
                                cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGt                                                                                                                          <  1:1191690/125‑1 (MQ=255)
                                cGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCa                                                                                                           >  1:2640318/1‑140 (MQ=255)
                                    tttCTGATGCGGAAAAGGAGATATTGCAAGTGACCCTCCGCCGCTTGGAAAGCATGTCTCCAAGATCAAAATTTATCTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGACAGCCTGGTGAGCTTTGAATCGCATGCg                                                                                                       <  1:1700981/140‑1 (MQ=255)
                                         gATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGt                                                                                                  <  1:2889900/140‑1 (MQ=255)
                                          aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                                                                                                 >  2:3929665/1‑140 (MQ=255)
                                          aTGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGtt                                                                                                 >  1:442932/1‑140 (MQ=255)
                                               gAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCa                                                                                            >  1:231900/1‑140 (MQ=255)
                                                aaaaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCaa                                                                                           <  1:1122461/140‑1 (MQ=255)
                                                  aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                                         <  2:3891529/140‑1 (MQ=255)
                                                  aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                                         <  1:3790424/140‑1 (MQ=255)
                                                  aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                                         <  2:1025196/140‑1 (MQ=255)
                                                  aaGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                                         <  2:1957052/140‑1 (MQ=255)
                                                  aaGGAGATATTGCAAGGCACCATACGCCGATTGGAAAGCCTGGCTCCAACATGAAAATTTATATGGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAaga                                                                                         <  2:2764855/140‑1 (MQ=255)
                                                     gagagaTCGCAAGTCACCATACACAGATTGGAAAGCCTGTTTCCAACACGAAAATTGATATTGTGGACCAGCATTTGAAGCCGGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAggc                                                                                      <  1:182576/140‑2 (MQ=255)
                                                     gagaTATTGCAATTCACCAGACGCCGAGTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                                      <  2:3413135/140‑1 (MQ=255)
                                                     gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTTCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                                      >  2:1764737/1‑140 (MQ=255)
                                                     gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                                      <  2:379511/140‑1 (MQ=255)
                                                     gagaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                                      >  2:2622602/1‑140 (MQ=255)
                                                       gagcggccAAGTCCCCCTACGCCGATTGGAAAGCCTGTCTCCAACCCGAACATTTATCCGGAGGCCCAGCCTTTCAAACCAGGCTCGATCGGAAACCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                                    <  1:519038/133‑1 (MQ=255)
                                                       gagatTGCAACTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                                    <  1:2719380/137‑1 (MQ=255)
                                                       gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                                    <  1:3876199/140‑1 (MQ=255)
                                                       gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                                    <  2:3983107/140‑1 (MQ=255)
                                                       gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                                    <  1:1928916/140‑1 (MQ=255)
                                                       gaTATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATa                                                                                    <  1:2013188/140‑1 (MQ=255)
                                                          atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                                      >  1:1530543/1‑135 (MQ=255)
                                                          atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                                                                 >  2:1413585/1‑140 (MQ=255)
                                                          atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                                                                 >  2:1675813/1‑140 (MQ=255)
                                                          atTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACta                                                                                 >  1:2935082/1‑140 (MQ=255)
                                                              cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATCCTataat                                                                             >  2:450576/1‑140 (MQ=255)
                                                              cAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATAGTGTGGACCAGCATTTGAAACCTGTGTCGATCGGCAAGCCGGGTGAGGTTTGCATCGCAGGCGACGGTTTGGCACGAGGAGACTctaat                                                                             >  2:128353/1‑140 (MQ=255)
                                                                aGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCCTTTGAAACCTGTGTCGATCGGAACGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTAtaataa                                                                           >  2:3580036/1‑140 (MQ=255)
                                                                  tCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGc                                                                         <  1:691795/140‑1 (MQ=255)
                                                                    aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                                                       <  2:646010/140‑1 (MQ=255)
                                                                    aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                                                       <  2:3491994/140‑1 (MQ=255)
                                                                    aCCATACACCGATTGGAAAGCCTGTTTCCAACACGAAAATTTATATTGTGGACCAGCATTTGAAGCCTGTCCCGATCGGAAAGCCAGGTGAGCTTTGCATCGCTGGAGCCGGCTTAGCAAGAGGCTACTTTAAAAAGCCg                                                                       <  2:2930419/140‑1 (MQ=255)
                                                                      cATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAAACTGTGTAGATCGGAAAGCATGGTTAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCggg                                                                     >  2:1376754/1‑140 (MQ=255)
                                                                               aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                                                            >  2:3222199/1‑140 (MQ=255)
                                                                               aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                                                            >  1:1020413/1‑140 (MQ=255)
                                                                               aTTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGc                                                            >  2:3611793/1‑140 (MQ=255)
                                                                                ttGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCt                                                           >  1:3555294/1‑140 (MQ=255)
                                                                                ttGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCt                                                           >  2:1408512/1‑140 (MQ=255)
                                                                                  ggAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGa                                                         <  2:1739237/140‑1 (MQ=255)
                                                                                   gacaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaa                                                        <  2:3654797/137‑1 (MQ=255)
                                                                                    aaaGCCTGTCTCCAACATGACAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCGTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTCTAATAAGCCGGGTCTTACGGCTGaaa                                                       <  2:3717814/140‑1 (MQ=255)
                                                                                    aaaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaa                                                       <  2:1157980/140‑1 (MQ=255)
                                                                                     aaGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGaaaa                                                      >  1:3025166/1‑140 (MQ=255)
                                                                                          tGTCTCCAACATGACAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCGTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAACTTta                                                 >  2:3153153/1‑140 (MQ=255)
                                                                                           gTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtat                                                >  1:935337/1‑140 (MQ=255)
                                                                                           gTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtat                                                >  1:2926832/1‑140 (MQ=255)
                                                                                            tctcCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTtata                                               <  1:1413585/140‑1 (MQ=255)
                                                                                                  aCATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAAc                                         >  2:1819616/1‑140 (MQ=255)
                                                                                                       aaaaTTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCAtt                                    <  2:533567/140‑1 (MQ=255)
                                                                                                             tctatTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                              <  2:2430459/138‑1 (MQ=255)
                                                                                                             tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                              <  1:3693913/140‑1 (MQ=255)
                                                                                                             tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                              <  1:1791608/140‑1 (MQ=255)
                                                                                                             tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                              <  1:3044605/140‑1 (MQ=255)
                                                                                                             tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                              <  2:2854470/140‑1 (MQ=255)
                                                                                                             tataTTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAAcc                              <  1:3929665/140‑1 (MQ=255)
                                                                                                                    tgGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTGTGGCAAGAGGATACTAGAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACa                       >  1:880733/1‑140 (MQ=255)
                                                                                                                       aCCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAg                    <  2:249629/140‑1 (MQ=255)
                                                                                                                       aCCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAg                    <  2:800151/140‑1 (MQ=255)
                                                                                                                          aGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCtt                 <  2:2246017/140‑1 (MQ=255)
                                                                                                                           gCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGAt                                                                                     <  1:607002/71‑1 (MQ=255)
                                                                                                                           gCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGAt                                                                                     >  2:607002/1‑71 (MQ=255)
                                                                                                                            cATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                                      >  1:3383660/1‑69 (MQ=255)
                                                                                                                            cATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGa                                                                                      <  2:3383660/69‑1 (MQ=255)
                                                                                                                            cATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTta               <  2:1538900/140‑1 (MQ=255)
                                                                                                                              tttGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTtata             >  2:8496/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TTACGGACCTACTGAAGCCTCCATTTACGCTGCGTATTTCGGATGCGGAAAAGGAGATATTGCAAGTCACCATACGCCGATTGGAAAGCCTGTCTCCAACATGAAAATTTATATTGTGGACCAGCATTTGAAACCTGTGTCGATCGGAAAGCCTGGTGAGCTTTGCATCGCAGGCGACGGTTTGGCAAGAGGATACTATAATAAGCCGGGTCTTACGGCTGAAAAATTTATAGACAACCCATTTGAACCAGGGACAAAGCTTTATAAATCGGGTGAT  >  NZ_CP034943/1805464‑1805740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: