Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,034,593 C→A 17.4% intergenic (‑259/‑135) EO946_RS10175 ← / → EO946_RS10180 UPF0715 family protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,034,5930CA17.4% 332.9 / 23.3 160intergenic (‑259/‑135)EO946_RS10175/EO946_RS10180UPF0715 family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (107/20);  new base A (2/31);  total (109/51)
Fisher's exact test for biased strand distribution p-value = 2.56e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

TAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATGA  >  NZ_CP034943/2034456‑2034731
                                                                                                                                          |                                                                                                                                           
      tggCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTGTCCCAGCTTTAGTCAGCTTCTTCATATACGACGTGTGCTAATAAAATGTTTCAAAAACGa                                                                                                                                    >  2:3231215/1‑140 (MQ=255)
      tggCTGTTATGTTTACATGCAGATTCCTTCCCTAACCGGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCATCTTCTTCATATACGAAGGGTGCTAATAAAATGTTTAAAAAACGa                                                                                                                                    >  2:869436/1‑140 (MQ=255)
        gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                  >  2:134829/1‑140 (MQ=255)
        gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                  >  1:1297403/1‑140 (MQ=255)
        gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                  >  2:3526600/1‑140 (MQ=255)
        gCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGAcc                                                                                                                                  >  2:1810893/1‑140 (MQ=255)
                 tttACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAg                                                                                                                         >  1:612803/1‑140 (MQ=255)
                 tttACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTGTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAg                                                                                                                         >  1:2298142/1‑140 (MQ=255)
                    aCATGCATTTTCCTCCCCTACCCTGTCTTTATCTCCGATTTTTTTTCAAAGGTTTTGTACCGTTTTTCCCCGCTTTCGTCATCTTCTACATATACGAAGTGTGATAAAAAAATGTTAAAAACACGACCGGGGTAAAGCTg                                                                                                                      <  2:2298142/140‑1 (MQ=255)
                     cATGCATATCCCTGCCCTAACCTGTCCTTATCTCCGTTTTTTTTCAAACCTTTTTTTACTGTGTTTCCCAGGTTTATTAAGATTCTTAATAAACCAAGTGTGCAAATAAATTTTTACAAAAACGACCGGGGTAAAGCTGa                                                                                                                     <  2:2100176/140‑1 (MQ=255)
                     cATGCAGTTTCCGTCCCTACCCGGTCCTTATCTCCGCTTCTTTTTCAACCGTTGGGTACTTTTTTCCCCAGCTTTAGTCAGCTTCTTAATATCCGACGGGTGATAATAAAATGTTACAAAAACGACCGGGGTAAAGCTGa                                                                                                                     <  2:754894/140‑1 (MQ=255)
                     attGCAGATTCCTTCCCTCACCTGTCCTTTTCTCCGTTTCTTTTTAAAACTTTTTGTACATTTTTCCCCAGCTTTCTTATCCTTATTAAAATCCCATGTGTGATAATAAACTTTATAAAAAACGACCGGGGTAAAGCTGa                                                                                                                     <  2:1569524/138‑1 (MQ=255)
                      aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGAc                                                                                                                    >  1:1722462/1‑140 (MQ=255)
                      aTGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGAc                                                                                                                    >  1:2221180/1‑140 (MQ=255)
                          attttccttccCTACCGTGTCCTTTTCTCCGTTTTTTTTTAAACGGTTTTTTACTGTTTTCCCCCGCTTCAGTCAGCTTCTACATATACGATGTTTGATATTAAATTGTTTAAAAAACGACCGGGGTAAAGCTGActctc                                                                                                                <  2:1349284/137‑1 (MQ=255)
                          ataTTCGTTCCCTACCCTGTCCTTTTCTCCGTTTCTTTTTCAAACTTTTTTTAACGTTTTTCCCAGCTTTAGTCAGCTTTTTCATATACTATGTGTGATAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGActctc                                                                                                                <  2:2094556/138‑1 (MQ=255)
                          aGATTCCTTCCCTAACCTGTCCTTATCCCCGATTATTTTTCAAAGGTTTTGTACTGTTTTTCCCAGCTTTAGACAGTTTCTAAATATACCAAGGTTGCTAATAAATTTTTTCAAAAACGACCGGGGTAAAGCTGActctc                                                                                                                <  2:3922340/140‑1 (MQ=255)
                           gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  1:2100176/1‑140 (MQ=255)
                           gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  1:974125/1‑140 (MQ=255)
                           gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  2:2578612/1‑140 (MQ=255)
                           gATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGActctct                                                                                                               >  2:2747285/1‑140 (MQ=255)
                            aTTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCtt                                                                                                              >  2:3192129/1‑140 (MQ=255)
                            aTTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCtt                                                                                                              >  2:1037045/1‑140 (MQ=255)
                              gccttccCAAACCTTACCTTATCTCCGCTTCTTTTAAAAACGTTTTGAACCGTGTTCCCACGCTTTAGTACGCTTCTACATTTCCGACGTGTGCTAATAAAATGTTCAAAACACGACCGGGGTAAAGCTGACTCTCTTGt                                                                                                            <  2:2437684/139‑1 (MQ=255)
                                  tccCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTa                                                                                                        >  2:384071/1‑140 (MQ=255)
                                  tccCTAACCTGTCCTTATCTCCGCTTCTTTTACAACCTTTTTGTACTTTGTTTCCCCGCTTTAGACAGCTTATTCATATACCACGTGTGATAAAAAAATTTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTa                                                                                                        <  2:1902058/140‑1 (MQ=255)
                                             tCCTTCTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCGTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:1764995/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGACagag                                                                                             >  1:3728269/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAcgag                                                                                             >  2:1127519/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  2:696957/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:2716414/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:1966551/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:411716/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAagag                                                                                             >  1:3458014/1‑140 (MQ=255)
                                             tCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACCAAGTGTGCTAATAAAAATTTTCAAAAACCACCGGGGTAAAGCTGACTATCTTGTCTTAATGGGAAatag                                                                                             >  1:350869/1‑140 (MQ=255)
                                                  aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAAc                                                                                        >  2:874108/1‑140 (MQ=255)
                                                  aTCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAAc                                                                                        >  2:126459/1‑140 (MQ=255)
                                                       cGCTTGTTTTTCAAACGGTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGTCTCTCTTGTCTTTATTGGAAAGAGTCAACGaaaa                                                                                   >  1:3347780/1‑140 (MQ=255)
                                                       cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTTTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  1:237880/1‑140 (MQ=255)
                                                       cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  1:4023908/1‑140 (MQ=255)
                                                       cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  2:2146999/1‑140 (MQ=255)
                                                       cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaa                                                                                   >  1:1257119/1‑140 (MQ=255)
                                                       cGCTTCTTTTTCAAACGTTTTGTACAGTGTTTACCCGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCCAAAAAGACAGGGGTAAATCTTACTCTCGTGTCTTCAAGGGAAAGAGTCAAAGaaaa                                                                                   >  1:584093/1‑140 (MQ=255)
                                                         cttcttTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGaaaaaa                                                                                 >  2:524498/1‑140 (MQ=255)
                                                           tatttttCAAACTTTTTGTACAGTTTTTCCCTGCTTTAGTAAGCTTCTACATATACGATGTGTGCTAATAAATTGTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAg                                                                               <  2:2710096/138‑1 (MQ=255)
                                                              ttttCAACCTTTTTTGACAGTGTTTCCAAGCTTTACTAAGCTTCTTACTATACGAAGGGTGATAATAAATTTTTTCAAAAACGACCGGGGTAAAACTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCga                                                                            <  2:411132/140‑1 (MQ=255)
                                                                       gtttttGTACTGTGTTTCCCAGCTTTATTCAGGTTCTTAATATACTCTTTGTGCTAATAAAATTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                    <  2:2341716/138‑1 (MQ=255)
                                                                       gtttttGTAACGTGTTTCACAGATTTAGTCAGCTTCTTAATATACGAGGTGTGATAATAAATTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                    <  2:1726809/138‑1 (MQ=255)
                                                                       gtttttGAACTGTGTTTCCATTATTTAGAAAGGTTATTAATAAACTATGTGTAATAATAAATTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                    <  1:3341522/138‑1 (MQ=255)
                                                                       ggtgttGTACAGGTTTGCCCTGTTTGTGTCTGCTTCTTCATATACGTCTGGTCCTAAAAAATTTTTAAACACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATAc                                                                    <  1:3341542/136‑1 (MQ=255)
                                                                         gtgtGTACCGTGTTACCCATCTTTAGTCATTTTCTTAATATACTATGTTTTATAATAAAATGTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCt                                                                  <  1:2264508/137‑1 (MQ=255)
                                                                                  actgttCCCATCTTTAGTCAGCTTCTTAATAAACGATGTTTGAAAATAAAATTTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacag                                                         <  2:1096094/136‑1 (MQ=255)
                                                                                   tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGGTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCGTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGACTGAATACCTGCCTAacaga                                                        >  1:3922237/1‑140 (MQ=255)
                                                                                   tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        >  1:2604939/1‑140 (MQ=255)
                                                                                   tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        >  1:1763339/1‑140 (MQ=255)
                                                                                   tgtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        >  1:749391/1‑140 (MQ=255)
                                                                                   tgtTGCCCAGTTTTATTCAGCTTCTACATATACTATGTGTGATTATAAAATTTTACAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacaga                                                        <  2:97421/140‑1 (MQ=255)
                                                                                    tttccccAGCTTTCGTCTGCTTTTCAATATCCGACGTGTGATAATAAAATTTTTAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                                                       <  2:1297403/136‑1 (MQ=255)
                                                                                    tttccccAGATTTCGTACGCTATTTAATATACGATTTTTGCTAATAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacagac                                                       <  2:2502375/136‑1 (MQ=255)
                                                                                    gtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAacctgt                                                       >  1:1050247/1‑136 (MQ=255)
                                                                                    gtTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAACCGAATGAATACCTGCCTAacctgt                                                       >  1:2178093/1‑136 (MQ=255)
                                                                                            tcTTTAGTACGCTTCTACATATACGATTTGTGCTAATAAATTTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaag                                               <  1:3192129/139‑1 (MQ=255)
                                                                                            gtttttttCAGCTTCTTCATATACCATTTTTGTTAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaag                                               <  1:321986/133‑1 (MQ=255)
                                                                                            gcttctgTCAGATTCTAAATATACGAAGTTTGATAATAAAATGTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaag                                               <  2:2186056/134‑1 (MQ=255)
                                                                                             cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAACGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              >  1:2532016/1‑140 (MQ=255)
                                                                                             cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              >  1:1531425/1‑140 (MQ=255)
                                                                                             cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              >  1:1530744/1‑140 (MQ=255)
                                                                                             cTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaaga                                              >  1:1359150/1‑140 (MQ=255)
                                                                                               ttACTCACCTTCTAAACATCCGAAGTGTGAAAATAAAATGTAACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATaagaag                                            <  1:3086482/140‑1 (MQ=255)
                                                                                                 aGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAagag                                          >  1:2063364/1‑140 (MQ=255)
                                                                                                       cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACTAACGTTGTAAAGCTGACTATCTTGTATTAAAGGTAAAAAGTCAACGAAAAAAAGCTAATGAATACCTCCATAAAAGACATAATAAGAAGAGTTGata                                    >  2:3947826/1‑140 (MQ=255)
                                                                                                       cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACCGACAGAATAAGAAGAGTTGata                                    >  2:3887930/1‑140 (MQ=255)
                                                                                                       cttcttCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGata                                    >  2:2037428/1‑140 (MQ=255)
                                                                                                         tcttaATATACGAAGTGTGATAATAAAATTTTTAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATAtt                                  <  2:2178971/140‑1 (MQ=255)
                                                                                                         tctccATATACGACTTGTGCTAATAAAATGTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATAtt                                  <  2:3061927/140‑1 (MQ=255)
                                                                                                            taatatACTATGTGTGATAAAAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCaa                               <  1:2152761/138‑1 (MQ=255)
                                                                                                                ataCTATGTGGGATAAAAAAATTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACt                           <  2:1799304/140‑1 (MQ=255)
                                                                                                                ataCGAAGTGTGCTAAGAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACt                           >  1:494260/1‑140 (MQ=255)
                                                                                                                 taCGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACtt                          >  2:157858/1‑140 (MQ=255)
                                                                                                                     aaGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTCACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCg                      >  1:1056682/1‑140 (MQ=255)
                                                                                                                     aaGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCg                      >  2:3812862/1‑140 (MQ=255)
                                                                                                                      aGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGACGAGTTGATATTCAACACTTTTCGt                     >  2:2061816/1‑140 (MQ=255)
                                                                                                                      aGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGt                     >  2:3427657/1‑140 (MQ=255)
                                                                                                                       gtgtGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTa                    >  2:1192602/1‑140 (MQ=255)
                                                                                                                        ttttataataaATTGTTCAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTaa                   <  1:64469/135‑1 (MQ=255)
                                                                                                                        tgtgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTaa                   >  2:3668091/1‑140 (MQ=255)
                                                                                                                        tgtgATAATAAATTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTaa                   <  1:2589696/140‑1 (MQ=255)
                                                                                                                         gttataataaACTTTTAAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTaaa                  <  2:3691590/136‑1 (MQ=255)
                                                                                                                          tgCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAg                 >  2:3345713/1‑140 (MQ=255)
                                                                                                                           tttaataaTTTTTTTAAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgg                <  1:239749/138‑1 (MQ=255)
                                                                                                                            cTAATAAAATGTTTCAAAAACGACCGGGTTAAAGCTGACTCTCTAGTCTTAAAGTGAAAGAGACAACGAAAAAAAGCGAATGAAAACCTGCCTAACATACAGAATAAGAAGAGGTGATATTCAACACTATTCGTAAAtgg               >  1:918684/1‑140 (MQ=255)
                                                                                                                            cTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGACAGAGTCAACGAAAAAAAGCGAATGAATACCTGACTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAggg               >  2:1742533/1‑140 (MQ=255)
                                                                                                                            cTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAggg               >  2:1492590/1‑140 (MQ=255)
                                                                                                                             taataaAATTTTACAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg              <  1:1831923/140‑1 (MQ=255)
                                                                                                                             aaataaATTTTTAAAAACACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAgggg              <  1:2747285/139‑1 (MQ=255)
                                                                                                                                taaAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGa           >  2:559155/1‑140 (MQ=255)
                                                                                                                                    aTGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACAcc       >  1:406532/1‑140 (MQ=255)
                                                                                                                                     ggTTACAACATCGACCGGGGTTAAGCTGAGTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCa      <  1:1277295/139‑1 (MQ=255)
                                                                                                                                        ttCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATg   <  1:1992944/140‑1 (MQ=255)
                                                                                                                                         tCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATGa  >  1:131518/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TAATGGTGGCTGTTATGTTTACATGCAGATTCCTTCCCTAACCTGTCCTTATCTCCGCTTCTTTTTCAAACGTTTTGTACAGTGTTTCCCAGCTTTAGTCAGCTTCTTCATATACGAAGTGTGCTAATAAAATGTTTCAAAAACGACCGGGGTAAAGCTGACTCTCTTGTCTTAATGGGAAAGAGTCAACGAAAAAAAGCGAATGAATACCTGCCTAACAGACAGAATAAGAAGAGTTGATATTCAACACTTTTCGTAAAGGGGTGACACCAATGA  >  NZ_CP034943/2034456‑2034731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: