Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,139,480 A→C 9.3% A119A (GCT→GCG deoD ← purine‑nucleoside phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,139,4800AC 9.3% 771.1 / 11.4 320A119A (GCT→GCGdeoDpurine‑nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (175/101);  new base C (3/41);  total (178/142)
Fisher's exact test for biased strand distribution p-value = 2.88e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

TGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATTCA  >  NZ_CP034943/2139343‑2139619
                                                                                                                                          |                                                                                                                                           
tGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATAGTTCCGAAAGc                                                                                                                                           >  1:3173520/1‑140 (MQ=255)
tGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGc                                                                                                                                           >  1:1035936/1‑140 (MQ=255)
 gCGAATCGTCGTTGTATAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCATTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCg                                                                                                                                          >  1:1032094/1‑140 (MQ=255)
          cgtTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTc                                                                                                                                 >  2:1958717/1‑140 (MQ=255)
           tttttAGAACTTATCAGCTTTAAATACGCTTCCTACCGGCACCGTCACGCCTTTATACTTTGCGGAATCATAGGAATTTTTTAAAAGCTCGAAATCTGCTCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCa                                                                                                                                <  2:4023709/136‑1 (MQ=255)
             tGTAGAACTGATCCGCTGTAAATAGGGTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATAATAGGCATTTTTTAAAAGCTCTAAATCTGCGAAAGGCGCAAAATAAATACTGCCCAAAGCGACTCTGTTCAtt                                                                                                                              <  2:1217932/140‑1 (MQ=255)
                  aaCTGATCAGATGTAAATACGCTACCAACCTTCACCGCCACGCCTTTATCATTTGCTGCATCAAAGGAATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGCAAAATAAATACTTCCGAAAGCGACTCTGTTCATTTGTGa                                                                                                                         <  1:2925358/140‑1 (MQ=255)
                   acggATCAGCTGTAAATACGCTGCCTACCGTCACCGGCACGCCTTTAGCCTTGGCGGCAACATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAAAACTTCCCAAAGCGACTCTGTTCATTTGTGaa                                                                                                                        <  2:1088186/137‑1 (MQ=255)
                   aCTGATCCGCTGTAAATACGCTTCATACCGTCACCGGCACGCCTTTATCCTTGGCGGCATCATCGGCTTTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGaa                                                                                                                        <  2:1888543/140‑1 (MQ=255)
                    cTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                       >  1:3567043/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCACGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAAt                                                                                                                       <  2:1128771/137‑1 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  2:279621/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:2388378/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:2969911/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:1101915/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:3667482/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:3819548/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:1990735/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:198284/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:3859850/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:1145018/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  2:614498/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:1361246/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCAGTTGTGAAGCAg                                                                                                                    >  2:812472/1‑140 (MQ=255)
                       aTCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGATCTAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAg                                                                                                                    >  1:1531721/1‑140 (MQ=255)
                            cTGTAAATACGCTTCCTACCGTCACAGGCAAGCCTTTATCCTTTGCGGCATCATAGGCATTTTTAAAAAGCTCGACATCTGCGCAAGGCGCAAAATCAATACTTCCGAACGCGACTCTTGTCATTTCTGAATCACTTGAg                                                                                                               >  2:2301459/1‑140 (MQ=255)
                             tttAAATACGCGTCCTCCCTCCCCCGGCAGGCCTTTATCCTTGGCGGAAACAAAGGAATTTTTAAAACGCGCGAACGCGGCGCACGGCGCAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                              <  1:2356992/138‑1 (MQ=255)
                             ttaaaaTACGCTTCCGACCGTCACCGGCACGCCTTTCTCCTTTGCGCAATCATAGGAATTTTTTAAAAGCTCGAAATCGGCGCAAGCCGCAAAACAAATACTTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                              <  1:3225773/137‑1 (MQ=255)
                             tGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                              <  2:2826303/140‑1 (MQ=255)
                             tGTAAATACGCTTCCTACCGTCACCGGCACGCCTGTATCCTTTGCGGCATCATAGGCATTTTTTAAACGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                              <  2:156288/140‑1 (MQ=255)
                             tGTAAATACGATTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGAATCATAGGCATTTTTTAAAAGCTCGAAATCGGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                              <  2:3043206/140‑1 (MQ=255)
                                 aaTACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGt                                                                                                          >  2:3281141/1‑140 (MQ=255)
                                    ccGCTTCCTCCCGTCACCGGCACGCCTTTATCCTTTGCGGACTCATAGGCATTTTTTAAAAGCTCGAAATCTGCCCAAGGCCAAAAACCAATACTCCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCAt                                                                                                       <  1:416792/139‑1 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAg                                                                                                                                            >  1:2083384/1‑101 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAg                                                                                                     >  1:930171/1‑140 (MQ=255)
                                      gCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGAATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAgg                                                                                                              <  2:745203/131‑1 (MQ=255)
                                         tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                  >  1:3050888/1‑140 (MQ=255)
                                         tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                  >  1:3477541/1‑140 (MQ=255)
                                         tCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                  >  1:2244664/1‑140 (MQ=255)
                                         tCCTACAGTCAACTGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGATCGAAATATGCGCAAGGCGCAAAATAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCa                                                                                                  >  2:2453965/1‑140 (MQ=255)
                                                  cACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGt                                                                                         >  1:1226217/1‑140 (MQ=255)
                                                    ccGGCCCGCCTTTATCCTTTGCGGCATCAGAGGCATTTTTTAAAACCTCGAAAGCTGCGCACGGCGCAAAATAAATACTTCCCAACGCGACTCGGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTcc                                                                                       <  1:3448248/140‑1 (MQ=255)
                                                    ccGGCACGCTTTTATCCTTTGGGCCTAACTCGGCTTTTTTTAAACGCTCTCATTCGGCCAATGGCGCAAAAACAATCCTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCAGAGCCAAAATGACGTcc                                                                                       <  1:2596120/140‑1 (MQ=255)
                                                      ggaccGCCTTTTTCATTTGCGGCTTATTAGGCATTTTTTAAAAGGTCGAAATCTGCGCACGGCGATAAATAAATACTTGCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCg                                                                                     <  2:3934858/136‑1 (MQ=255)
                                                       gCACGCCTTTATCCTTGGCGGCATCATCGGAATTTTTTAAACGCTCGAAATCTGCGCAAGGCGAAAACTCAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGc                                                                                    <  1:1723631/140‑1 (MQ=255)
                                                          cGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACt                                                                                 >  1:1584179/1‑140 (MQ=255)
                                                           gCCTTTCGCCGTGGCGGCATCATCGGAATTTTTTAAAAGCTCTAAATCGGCGCAAGGCGCAAAATCAATACTTCCGACCGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACtt                                                                                <  1:580213/140‑1 (MQ=255)
                                                           gCCTTTATCCTTGGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCGGCGCACGGCGCAAAATCAATACTGCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACtt                                                                                <  1:437413/140‑1 (MQ=255)
                                                                tATCCTTTGCGGCATCATAGTCATTTTTTAAAACCTCGAAATCTGCGCAAGGCGAAAAATAAATACTTCCTAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACa                                                                           <  2:1737595/140‑1 (MQ=255)
                                                                    cTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGACAAAATGACGTCCCGCACTTTGACATCtt                                                                       >  1:3116866/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGCAAAATCAAAACTTCCGAAAGCGACTCTGTTCATTTGTGAATAAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:3605816/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:2106435/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:1424477/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:175226/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:960083/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:1849917/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:241092/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:2528725/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:62213/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:1509420/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:2963648/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:3551007/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:2277592/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  1:1457823/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGc                                                                    >  2:742853/1‑140 (MQ=255)
                                                                       tGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTGTGACATCTTTGc                                                                    >  2:3149784/1‑140 (MQ=255)
                                                                            catcatAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATa                                                               >  1:3080926/1‑140 (MQ=255)
                                                                             atcatAGGAATTTTTTAAACGCTCGAAATCGGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAg                                                              <  2:3667482/140‑1 (MQ=255)
                                                                               catAGGCATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                            <  2:1382864/140‑1 (MQ=255)
                                                                               catAGGAATTTTTAAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                            <  2:2033435/140‑1 (MQ=255)
                                                                               aatatGCATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                            <  2:3347834/139‑1 (MQ=255)
                                                                                aatacccATTTTTTAAAAGCTCTAATTGTGCCCAACGCTAAAAATAAATACTTCCTAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAgcg                                                            <  1:64831/134‑1 (MQ=255)
                                                                                     tcATTTTTTAAAAGCTCTAAATCTGCGCAAGGCGAAAAATCAATACTTCCGAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                       <  2:3116866/139‑1 (MQ=255)
                                                                                     taaTTTTTTAAAAGATCTAAATCTGCTAAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                       <  1:869795/138‑1 (MQ=255)
                                                                                     gaaTTTTTTAAAATCTCTAAATCTGCGCAAGGCGCAAAATAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                       <  2:2215849/138‑1 (MQ=255)
                                                                                     gCATTTTTAAAAAGCTCTAAATCTGCGCAAGGCGCAAAATAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                       <  2:3891101/140‑1 (MQ=255)
                                                                                     gCATTTTGTAAACGCTCGAACTCTGCGCAAGGCGAAAAATCAATACTTCCGAAAGCGACTCTGGTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCa                                                       <  1:3126542/140‑1 (MQ=255)
                                                                                          ttttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAAc                                                  >  1:3396973/1‑140 (MQ=255)
                                                                                           tttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAAcc                                                 >  1:594465/1‑140 (MQ=255)
                                                                                           tttAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAAcc                                                 >  1:518334/1‑140 (MQ=255)
                                                                                                     cGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                                       <  2:2388378/140‑1 (MQ=255)
                                                                                                     cGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCttatt                                       <  2:1310400/140‑1 (MQ=255)
                                                                                                             gcgcATGGCGAAAAAAAAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTg                               <  1:1261166/140‑1 (MQ=255)
                                                                                                               gcAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                             <  2:3444864/140‑1 (MQ=255)
                                                                                                               gcAAGGCGCAAAAACAATCCTTCCCAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                             <  1:2030187/140‑1 (MQ=255)
                                                                                                               ccAAGGCGAAAAATCAATACTTCCCAACGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGca                             <  2:424100/139‑1 (MQ=255)
                                                                                                                  aGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACAt                          >  1:388865/1‑140 (MQ=255)
                                                                                                                  aGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACAt                          >  1:1786396/1‑140 (MQ=255)
                                                                                                                       cAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAg                     >  1:1818359/1‑140 (MQ=255)
                                                                                                                         aaaTCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCt                   <  2:3497317/140‑1 (MQ=255)
                                                                                                                              aaaaCTTCCCAAAGCGACTCTTTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaa              <  2:1604075/137‑1 (MQ=255)
                                                                                                                              aaTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaa              <  1:2689926/140‑1 (MQ=255)
                                                                                                                               aTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaat             >  1:3178322/1‑140 (MQ=255)
                                                                                                                               aTACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGaat             >  1:251314/1‑140 (MQ=255)
                                                                                                                                     ccGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattc       >  1:3781229/1‑140 (MQ=255)
                                                                                                                                     ccGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattc       >  1:2761508/1‑140 (MQ=255)
                                                                                                                                        aaaGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattcatt    <  1:3308852/140‑1 (MQ=255)
                                                                                                                                        aaaGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAattcatt    <  1:788079/140‑1 (MQ=255)
                                                                                                                                          aGCGACTCTGTTCATTTTTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACACTTATTAGATTTTTCACATAGTAGCTTTGAAATAAttcattca  >  2:1465031/1‑140 (MQ=255)
                                                                                                                                          aGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACCTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAAttcattca  >  2:2673623/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TGCGAATCGTCGTTGTAGAACTGATCAGCTGTAAATACGCTTCCTACCGTCACCGGCACGCCTTTATCCTTTGCGGCATCATAGGCATTTTTTAAAAGCTCGAAATCTGCGCAAGGCGCAAAATCAATACTTCCGAAAGCGACTCTGTTCATTTGTGAATCAGTTGAGGATGTCATAGCCAAAATGACGTCCCGCACTTTGACATCTTTGCGGATAGCGCCGCAAGAACCTACTCTTATTAGATTTTGCACATCGTAGCTTTGAATTAATTCATTCA  >  NZ_CP034943/2139343‑2139619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: